NO992107L - Method for detecting compounds that modulate the effect of the fat (OB) protein - Google Patents

Method for detecting compounds that modulate the effect of the fat (OB) protein

Info

Publication number
NO992107L
NO992107L NO992107A NO992107A NO992107L NO 992107 L NO992107 L NO 992107L NO 992107 A NO992107 A NO 992107A NO 992107 A NO992107 A NO 992107A NO 992107 L NO992107 L NO 992107L
Authority
NO
Norway
Prior art keywords
cell line
protein
derived cell
compound
response element
Prior art date
Application number
NO992107A
Other languages
Norwegian (no)
Other versions
NO992107D0 (en
Inventor
Lee James Beeley
Original Assignee
Smithkline Beecham Plc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9622870.5A external-priority patent/GB9622870D0/en
Priority claimed from GBGB9622866.3A external-priority patent/GB9622866D0/en
Priority claimed from GBGB9622868.9A external-priority patent/GB9622868D0/en
Priority claimed from GBGB9622850.7A external-priority patent/GB9622850D0/en
Priority claimed from GBGB9622869.7A external-priority patent/GB9622869D0/en
Priority claimed from GBGB9622847.3A external-priority patent/GB9622847D0/en
Priority claimed from GBGB9622865.5A external-priority patent/GB9622865D0/en
Priority claimed from GBGB9622849.9A external-priority patent/GB9622849D0/en
Priority claimed from GBGB9622867.1A external-priority patent/GB9622867D0/en
Priority claimed from GBGB9622851.5A external-priority patent/GB9622851D0/en
Priority claimed from GBGB9622848.1A external-priority patent/GB9622848D0/en
Application filed by Smithkline Beecham Plc filed Critical Smithkline Beecham Plc
Publication of NO992107D0 publication Critical patent/NO992107D0/en
Publication of NO992107L publication Critical patent/NO992107L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/5759—Products of obesity genes, e.g. leptin, obese (OB), tub, fat
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Endocrinology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Child & Adolescent Psychology (AREA)
  • Obesity (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Det beskrives en fremgangsmåte for påvisning av en forbindelse som etterligner, potensierer eller inhiberer den fysiologiske effekt av ob-proteinet, og som omfatter: (a) for en forbindelse som etterligner den fysiologiske effekt av ob- proteinet, bestemmelse av effekten av forbindelsen på et ob-proteinaktivert STAT- DNA responselement koblet til et rapportørgen (STAT = signaltransducer og aktivator av transkripsjon; eller (b) for en forbindelse som potensierer eller inhiberer den fysiologiske effekt av ob-proteinet, bestemmelse av effekten av forbindelsen på den respons som gis av ob-protein på et ob-proteinaktivert STAT DNA- responselement koblet til et rapportørgen; hvor responselementet og rapportøren uttrykkes i en ob-proteinresponderende cellelinje, hvor cellelinjen er valgt fra følgende liste: en hypothalamus-avledet cellelinje; en feokromocytom-avledet cellelinje; en hematopoetisk avledet cellelinje; en pankreatisk avledet cellelinje; en lever-avledet cellelinje; en preadipocytt-avledet cellelinje; en skjelettmuskel-avledet cellelinje; og en ovarie-avledet cellelinje; eller responselementet og rapportøren uttrykkes i en cellelinje, hvor cellelinjen (den manipulerte cellelinje) også transfekteres med et polypeptid som er i stand til å mediere stimuleringen av ob-protein av et ob- proteinaktivert STAT DNA-responselement og inneholder de passende STAT- proteinene.A method is disclosed for detecting a compound that mimics, potentiates or inhibits the physiological effect of the ob protein, and comprising: (a) for a compound that mimics the physiological effect of the ob protein, determining the effect of the compound on a ob-protein-activated STAT DNA response element coupled to a reporter gene (STAT = signal transducer and transcription activator; or (b) for a compound that potentiates or inhibits the physiological effect of the ob-protein, determining the effect of the compound on the response given by ob-protein on an ob-protein-activated STAT DNA response element linked to a reporter gene, wherein the response element and reporter are expressed in an ob-protein-responsive cell line, where the cell line is selected from the following list: a hypothalamus-derived cell line; a pheochromocytoma-derived cell line; hematopoietically derived cell line; a pancreatically derived cell line; a liver-derived cell line; a pre adipocyte-derived cell line; a skeletal muscle-derived cell line; and an ovarian-derived cell line; or the response element and reporter are expressed in a cell line, wherein the cell line (the engineered cell line) is also transfected with a polypeptide capable of mediating the stimulation of ob protein by an ob protein activated STAT DNA response element and containing the appropriate STAT proteins.

NO992107A 1996-11-01 1999-04-30 Method for detecting compounds that modulate the effect of the fat (OB) protein NO992107L (en)

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
GBGB9622850.7A GB9622850D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622869.7A GB9622869D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622847.3A GB9622847D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622870.5A GB9622870D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622848.1A GB9622848D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622866.3A GB9622866D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622851.5A GB9622851D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622867.1A GB9622867D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622849.9A GB9622849D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622865.5A GB9622865D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622868.9A GB9622868D0 (en) 1996-11-01 1996-11-01 Novel method
PCT/GB1997/002988 WO1998020158A1 (en) 1996-11-01 1997-10-30 Method for the detection of compounds that modulate the effects of the obese (ob) protein

Publications (2)

Publication Number Publication Date
NO992107D0 NO992107D0 (en) 1999-04-30
NO992107L true NO992107L (en) 1999-06-30

Family

ID=27581686

Family Applications (1)

Application Number Title Priority Date Filing Date
NO992107A NO992107L (en) 1996-11-01 1999-04-30 Method for detecting compounds that modulate the effect of the fat (OB) protein

Country Status (13)

Country Link
EP (1) EP0951564A1 (en)
JP (1) JP2001503982A (en)
KR (1) KR20000053007A (en)
AR (1) AR010262A1 (en)
AU (1) AU4789297A (en)
CA (1) CA2270431A1 (en)
CZ (1) CZ149999A3 (en)
IL (1) IL129685A0 (en)
NO (1) NO992107L (en)
NZ (1) NZ335489A (en)
PL (1) PL333187A1 (en)
TR (1) TR199900963T2 (en)
WO (1) WO1998020158A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1293533B1 (en) * 1997-07-14 1999-03-01 Angeletti P Ist Richerche Bio METHOD FOR THE SELECTION OF MOLECULES ABLE TO MIMIC, INHIBIT OR ENHANCE THE EFFECTS OF THE INTERACTION BETWEEN LEPTIN AND CELLS THAT
GB9814199D0 (en) * 1998-06-30 1998-08-26 Univ Buckingham Novel method
EP1101112B1 (en) * 1998-07-28 2004-10-06 Vlaams Interuniversitair Instituut voor Biotechnologie vzw. Leptin-mediated gene-induction
GB9828087D0 (en) * 1998-12-18 1999-02-17 Smithkline Beecham Plc Novel method
EP1612221A3 (en) 2000-05-22 2008-07-23 Vlaams Interuniversitair Instituut voor Biotechnologie vzw. Receptor-based interaction trap
GB0019327D0 (en) * 2000-08-08 2000-09-27 Buchanan Baillie Hamilton Paul Slimming system
EP1812038A1 (en) 2004-11-18 2007-08-01 VIB vzw Fibronectin iii domain as leptin receptor antagonists

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5643748A (en) * 1994-09-14 1997-07-01 Progenitor, Inc. HU-B1.219, a novel human hematopoietin receptor
WO1996029405A2 (en) * 1995-03-20 1996-09-26 Ligand Pharmaceuticals Incorporated MODULATORS OF ob GENE AND SCREENING METHODS THEREFOR
US5712094A (en) * 1995-03-27 1998-01-27 Ligand Pharmaceuticals, Inc. Methods for detecting modulators of cytokine action
CA2222409A1 (en) * 1995-05-30 1996-12-05 Smithkline Beecham P.L.C. Method for the detection of compounds that modulate the effects of the obese protein
CA2252443A1 (en) * 1996-04-22 1997-10-30 Merck & Co., Inc. Leptin assay

Also Published As

Publication number Publication date
IL129685A0 (en) 2000-02-29
NZ335489A (en) 2001-01-26
TR199900963T2 (en) 1999-08-23
KR20000053007A (en) 2000-08-25
CZ149999A3 (en) 1999-10-13
WO1998020158A1 (en) 1998-05-14
AU4789297A (en) 1998-05-29
JP2001503982A (en) 2001-03-27
PL333187A1 (en) 1999-11-22
EP0951564A1 (en) 1999-10-27
CA2270431A1 (en) 1998-05-14
AR010262A1 (en) 2000-06-07
NO992107D0 (en) 1999-04-30

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