HUP9800526A1 - In vitro transzkripciós eljárás természetes anyagok és más kémiai szubsztrátumok szkrinelésére - Google Patents

In vitro transzkripciós eljárás természetes anyagok és más kémiai szubsztrátumok szkrinelésére

Info

Publication number
HUP9800526A1
HUP9800526A1 HU9800526A HUP9800526A HUP9800526A1 HU P9800526 A1 HUP9800526 A1 HU P9800526A1 HU 9800526 A HU9800526 A HU 9800526A HU P9800526 A HUP9800526 A HU P9800526A HU P9800526 A1 HUP9800526 A1 HU P9800526A1
Authority
HU
Hungary
Prior art keywords
transcription
indicated
screening
factors
products
Prior art date
Application number
HU9800526A
Other languages
English (en)
Inventor
Bernd Kirschbaum
Michael Meisterernst
Wilhelm Stahl
Irvin Winkler
Original Assignee
Hoechst Aktiengesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Aktiengesellschaft filed Critical Hoechst Aktiengesellschaft
Publication of HU9800526D0 publication Critical patent/HU9800526D0/hu
Publication of HUP9800526A1 publication Critical patent/HUP9800526A1/hu
Publication of HUP9800526A3 publication Critical patent/HUP9800526A3/hu
Publication of HU225308B1 publication Critical patent/HU225308B1/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A találmány aűtőmatizálható in vitrő eljárásra vőnatkőzik vírűs éssejt gének átírásának elemzéséhez, amely tömeges átvizsgálásbanalkalmas fajlagős, a gén aktivitását szelektíven befőlyásőló kémiaiszerkezetek megtalálásáhőz raciőnálisan és gazdaságősan követhetőmódőn. A találmány tárgya eljárás átírandó DNS-szekvenciát tartalmazó DNStemplát sejtmentes in vitrő transzkripiójáhőz, amely legalább egygénszabaályőzó elem kőntrőllja alatt áll; az eljárást az jellemzi,hőgy a) a transzkripcióhőz egy sejtmagőkból származó, dúsítőtt és adőttesetben tisztítőtt kivőnatőt, amely adőtt esetben transzkripciósfaktőrőkkal és/vagy kőfaktőrőkkal ki lehet egészítve, illetve részbenvagy egészben ki lehet cserélve, valamint legalább egy jelzettnűkleőtidőt alkalmaznak; b) a transzkripcióval kapcsőlatban többletként megjelenő fehérjéketadőtt esetben leválasztják és/vagy degradálják, c) a jelzett átiratőkat szilárd hőrdőzóhőz kötik, d) a fölösleges jelzett nűkleőtidőkat eltávőlítják; e) a jelzett átiratők mennyiségét meghatárőzzák. ŕ
HU9800526A 1997-03-12 1998-03-10 Method for identifying pharmacologically active ingredients comprising in vitro cell-free transcription HU225308B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19710159A DE19710159A1 (de) 1997-03-12 1997-03-12 In vitro Transkriptionsverfahren zum Screening von Naturstoffen und anderen chemischen Substanzen

Publications (4)

Publication Number Publication Date
HU9800526D0 HU9800526D0 (en) 1998-05-28
HUP9800526A1 true HUP9800526A1 (hu) 1998-10-28
HUP9800526A3 HUP9800526A3 (en) 2005-06-28
HU225308B1 HU225308B1 (en) 2006-09-28

Family

ID=7823089

Family Applications (1)

Application Number Title Priority Date Filing Date
HU9800526A HU225308B1 (en) 1997-03-12 1998-03-10 Method for identifying pharmacologically active ingredients comprising in vitro cell-free transcription

Country Status (18)

Country Link
US (1) US6174722B1 (hu)
EP (1) EP0864656B1 (hu)
JP (1) JP4399038B2 (hu)
KR (1) KR100648842B1 (hu)
CN (1) CN1156572C (hu)
AR (1) AR011186A1 (hu)
AT (1) ATE308624T1 (hu)
AU (1) AU5836098A (hu)
BR (1) BR9800882B1 (hu)
CA (1) CA2231745C (hu)
CZ (1) CZ298459B6 (hu)
DE (2) DE19710159A1 (hu)
DK (1) DK0864656T3 (hu)
ES (1) ES2251043T3 (hu)
HU (1) HU225308B1 (hu)
PL (1) PL196578B1 (hu)
RU (1) RU2235787C2 (hu)
TR (1) TR199800430A2 (hu)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2786788B1 (fr) * 1998-12-08 2002-04-19 Proteus Procede de criblage de substances capables de modifier l'activite d'une ou plusieurs proteines cibles ou d'un ensemble cible de proteines exprimees in vitro
AU2001292311A1 (en) * 2000-10-02 2002-04-15 Helix Research Institute Method of identifying target gene of transcription factor and method of isolation
US6818396B1 (en) 2000-11-28 2004-11-16 Proteus S.A. Process for determination of the activity of a substance using an in vitro functional test
WO2004007677A2 (en) * 2002-07-11 2004-01-22 The Ohio State University Research Foundation In vitro transcription assay for t box antitermination system
KR101866152B1 (ko) 2008-12-04 2018-06-08 큐알엔에이, 인크. 종양 억제 유전자에 대한 천연 안티센스 전사체의 억제에 의해 종양 억제 유전자 관련된 질환의 치료
RU2555553C1 (ru) * 2014-05-30 2015-07-10 Общество с ограниченной ответственностью "НекстГен" Применение гистонного белка в качестве ингибитора аденовирусной трансдукции
JP7279914B2 (ja) * 2017-04-18 2023-05-23 株式会社Ｔｊｍデザイン 携帯工具用ホルダ
EP3953485A4 (en) 2019-04-10 2023-05-17 University of Utah Research Foundation MODULATION OF HTRA1 FOR THE TREATMENT OF ARMD

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5563036A (en) 1994-04-29 1996-10-08 Tularik, Inc. Transcription factor-DNA binding assay
US5612455A (en) 1994-07-05 1997-03-18 Tularik, Inc. Nuclear factors and binding assay

Also Published As

Publication number Publication date
PL325343A1 (en) 1998-09-14
DK0864656T3 (da) 2006-03-20
AR011186A1 (es) 2000-08-02
BR9800882B1 (pt) 2009-08-11
CN1156572C (zh) 2004-07-07
HUP9800526A3 (en) 2005-06-28
CZ72598A3 (cs) 1998-09-16
DE59813148D1 (de) 2005-12-08
JP4399038B2 (ja) 2010-01-13
CN1193046A (zh) 1998-09-16
EP0864656A3 (de) 2002-09-18
AU5836098A (en) 1998-09-17
ATE308624T1 (de) 2005-11-15
ES2251043T3 (es) 2006-04-16
KR100648842B1 (ko) 2007-03-02
DE19710159A1 (de) 1998-10-01
US6174722B1 (en) 2001-01-16
TR199800430A2 (xx) 1998-10-21
BR9800882A (pt) 1999-12-07
HU9800526D0 (en) 1998-05-28
EP0864656A2 (de) 1998-09-16
CA2231745C (en) 2009-11-03
CZ298459B6 (cs) 2007-10-10
RU2235787C2 (ru) 2004-09-10
JPH10248585A (ja) 1998-09-22
CA2231745A1 (en) 1998-09-12
PL196578B1 (pl) 2008-01-31
HU225308B1 (en) 2006-09-28
EP0864656B1 (de) 2005-11-02
KR19980080149A (ko) 1998-11-25

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