HUE035508T2 - Eljárások növelt proteintermelésre - Google Patents

Eljárások növelt proteintermelésre Download PDF

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Publication number
HUE035508T2
HUE035508T2 HUE10782133A HUE10782133A HUE035508T2 HU E035508 T2 HUE035508 T2 HU E035508T2 HU E10782133 A HUE10782133 A HU E10782133A HU E10782133 A HUE10782133 A HU E10782133A HU E035508 T2 HUE035508 T2 HU E035508T2
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HU
Hungary
Prior art keywords
cells
cell
cell culture
továbbá
továbbá továbbá
Prior art date
Application number
HUE10782133A
Other languages
English (en)
Inventor
Alahari Arunakumari
Xiao-Ping Dai
Javier Garcia
Richard Martel
Original Assignee
Squibb & Sons Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43480207&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=HUE035508(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Squibb & Sons Llc filed Critical Squibb & Sons Llc
Publication of HUE035508T2 publication Critical patent/HUE035508T2/hu

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Claims (2)

  1. PíPŐaáabpí ρΡίΡΆ §. s j;ásás;pmtsfn növekvő termeissére ssj-tényöszstbets, amely tsrtaimaz: a) áejsék ssővésztésés, ásáejyek iérrnslík a pfétefen pezőizáás séjöenyész.eibes'í legalább So · $ íj" sejt/mi feledi se j s sä rá ség y; é s hy » sejtek ;st\S·V ;<:{ séd -besd· sejSieisyészeiôé, égy hesgy is sejtek ig-'ssttsess lázispss Srperk: ab<> 1 s :s«pek állats sejtek. X V \ X >1 -.ül S 'S ö Xi <1 V pep ! v V. t,lk( ,! X 3 x fj, >V,;. \ , ; sáráséesa kötölbelöf SOxlO°sejt/n)i és i >ö* Ü)” sep/tni közöst. X Az I vagy· 2, igény pont szerhsti eljárás, sássá a sejtek a períázbss sej-testyes.zesbe-s vannak aővs;szsve köridheljsí I öő l(y sejsbpl tekéi ssíjssöréségíg.
  2. 4, Ág előző i .ekeypsyttok bárspelyiks szefirsit eljárás, ííisrsj a proseás spstiíKS: :>, Az előző tgestypöpíök bétsáéiylke SZárisál elsásás, sshol ;s pröiesp ki válaszAa « következőkből álló csoportból; eazinsék, reeepsesktk. pizlős pteseistek. eástkásek, szabélyezásj ikktóröig iserrPOnök, spsjgéssköső stgessstek, terápiás proíeinők.. és á ssy.ee erük ex pszíteírsek, b, Az előző Igénypöistsjk késesekike Szerinti eijársky ehet a períázlsA sejttenyészeí sd iesl-lsdsb sejttenyészet egye; lm bieszsikSPsbast visít növesztve. X Az 1-5. igénypontok bármelyike szerinti eljsáás, shrsl &amp; perfúziós sejslessyészet és lecl-lsasei: sejltessypszet kőibe bioreaktorokban vsspsssk stőveszíve, &amp;. Az AS. és ?, tgéssypggsök bársgiáyjke vzerínts eljárás, éhei e fed-bísísts síásentés/e! több őistressk-sss'őssís vsa assvszztvr·. ék Az 7, vagy s. igÿpy pots! sze; ti sgi eljárás, eátály továbbá setts j t ssíszzá á pressas <g,;öltését a féd-batch séjttenyészeiböl és néns ss perfözfós sejstenyészeibök 1Ő. A Ö„ igénypont szerbit! eljárás. áirseiy továbbá k-rpésesz/s is ptötésb gyűjtését niind a perfözíéa t-'ptenytn/esKn x's tíse't t seb-Xeeh vdtsenyx; ·όΑρ
HUE10782133A 2009-11-17 2010-11-17 Eljárások növelt proteintermelésre HUE035508T2 (hu)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US26188609P 2009-11-17 2009-11-17

Publications (1)

Publication Number Publication Date
HUE035508T2 true HUE035508T2 (hu) 2018-05-02

Family

ID=43480207

Family Applications (1)

Application Number Title Priority Date Filing Date
HUE10782133A HUE035508T2 (hu) 2009-11-17 2010-11-17 Eljárások növelt proteintermelésre

Country Status (14)

Country Link
US (2) US8765415B2 (hu)
EP (3) EP2501822B1 (hu)
CY (1) CY1119923T1 (hu)
DK (1) DK2501822T3 (hu)
ES (1) ES2642629T3 (hu)
HR (1) HRP20171701T8 (hu)
HU (1) HUE035508T2 (hu)
LT (1) LT2501822T (hu)
NO (1) NO2501822T3 (hu)
PL (1) PL2501822T3 (hu)
PT (1) PT2501822T (hu)
RS (1) RS56484B1 (hu)
SI (1) SI2501822T1 (hu)
WO (1) WO2011062926A2 (hu)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2188371B1 (en) * 2007-08-09 2017-12-20 Wyeth LLC Use of perfusion to enhance production of fed-batch cell culture in bioreactors
US8691145B2 (en) 2009-11-16 2014-04-08 Flodesign Sonics, Inc. Ultrasound and acoustophoresis for water purification
DK2501822T3 (da) * 2009-11-17 2017-11-27 Squibb & Sons Llc Fremgangsmåder til forbedret proteinproduktion
US8506797B2 (en) * 2011-04-10 2013-08-13 Therapeutic Proteins International, LLC Downstream bioprocessing device
CN103827292B (zh) 2011-07-01 2018-01-26 安姆根有限公司 哺乳动物细胞培养
US8852435B2 (en) * 2011-11-29 2014-10-07 Therapeutics Proteins International, LLC Purification and separation treatment assembly (PASTA) for biological products
US10370635B2 (en) 2012-03-15 2019-08-06 Flodesign Sonics, Inc. Acoustic separation of T cells
US9752113B2 (en) 2012-03-15 2017-09-05 Flodesign Sonics, Inc. Acoustic perfusion devices
US10953436B2 (en) 2012-03-15 2021-03-23 Flodesign Sonics, Inc. Acoustophoretic device with piezoelectric transducer array
US9272234B2 (en) 2012-03-15 2016-03-01 Flodesign Sonics, Inc. Separation of multi-component fluid through ultrasonic acoustophoresis
US9752114B2 (en) 2012-03-15 2017-09-05 Flodesign Sonics, Inc Bioreactor using acoustic standing waves
US10322949B2 (en) 2012-03-15 2019-06-18 Flodesign Sonics, Inc. Transducer and reflector configurations for an acoustophoretic device
US10689609B2 (en) 2012-03-15 2020-06-23 Flodesign Sonics, Inc. Acoustic bioreactor processes
US9950282B2 (en) 2012-03-15 2018-04-24 Flodesign Sonics, Inc. Electronic configuration and control for acoustic standing wave generation
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
US9796956B2 (en) 2013-11-06 2017-10-24 Flodesign Sonics, Inc. Multi-stage acoustophoresis device
US9783775B2 (en) 2012-03-15 2017-10-10 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US9567559B2 (en) 2012-03-15 2017-02-14 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US9745548B2 (en) 2012-03-15 2017-08-29 Flodesign Sonics, Inc. Acoustic perfusion devices
US10967298B2 (en) 2012-03-15 2021-04-06 Flodesign Sonics, Inc. Driver and control for variable impedence load
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US10737953B2 (en) 2012-04-20 2020-08-11 Flodesign Sonics, Inc. Acoustophoretic method for use in bioreactors
TWI637057B (zh) * 2012-11-09 2018-10-01 拜爾沙納有限公司 具交替生物反應器用途之不連續進料批次製程
US9745569B2 (en) 2013-09-13 2017-08-29 Flodesign Sonics, Inc. System for generating high concentration factors for low cell density suspensions
JP6683604B2 (ja) * 2013-09-30 2020-04-22 ヤンセン ファッシンズ アンド プリベンション ベーフェーＪａｎｓｓｅｎ Ｖａｃｃｉｎｅｓ ＆ Ｐｒｅｖｅｎｔｉｏｎ Ｂ．Ｖ． 高密度粗製細胞培養回収物の清澄化のための方法
WO2015105955A1 (en) 2014-01-08 2015-07-16 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
CA2939872A1 (en) * 2014-02-17 2015-08-20 Advantech Bioscience Farmaceutica Ltda Enhancement of recombinant protein expression using a membrane-based cell retention system
EP3119871A4 (en) * 2014-03-17 2018-01-03 Regeneron Pharmaceuticals, Inc. Clarification of mammalian cell culture
WO2015143512A2 (en) * 2014-03-23 2015-10-01 Advantech Bioscience Farmacêutica Ltda. Enhancement of recombinant protein expression with copper
US9855319B2 (en) 2014-04-01 2018-01-02 Advantech Bioscience Farmacêutica Ltda Stabilization of factor VIII without calcium as an excipient
WO2015149143A2 (en) 2014-04-01 2015-10-08 Advantech Bioscience Farmacêutica Ltda. Stable factor viii formulations with low sugar-glycine
US9744483B2 (en) 2014-07-02 2017-08-29 Flodesign Sonics, Inc. Large scale acoustic separation device
CN105296433B (zh) 2014-08-01 2018-02-09 中山康方生物医药有限公司 一种ctla4抗体、其药物组合物及其用途
CA2971861C (en) * 2014-12-22 2024-02-13 Genzyme Corporation Methods of culturing a mammalian cell
US10106770B2 (en) 2015-03-24 2018-10-23 Flodesign Sonics, Inc. Methods and apparatus for particle aggregation using acoustic standing waves
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11021699B2 (en) 2015-04-29 2021-06-01 FioDesign Sonics, Inc. Separation using angled acoustic waves
CN108138128A (zh) * 2015-05-29 2018-06-08 葛莱高托普有限公司 用于悬浮细胞的小规模养殖方法
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
US10710006B2 (en) 2016-04-25 2020-07-14 Flodesign Sonics, Inc. Piezoelectric transducer for generation of an acoustic standing wave
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
CN114891635A (zh) 2016-05-03 2022-08-12 弗洛设计声能学公司 利用声泳的治疗细胞洗涤、浓缩和分离
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
WO2018035710A1 (en) 2016-08-23 2018-03-01 Akeso Biopharma, Inc. Anti-ctla4 antibodies
CN110494543A (zh) 2016-10-19 2019-11-22 弗洛设计声能学公司 通过声学的亲和细胞提取
KR20200068697A (ko) 2017-10-06 2020-06-15 론자 리미티드 라만 분광법을 사용하는 세포 배양의 자동 제어
CN118580926A (zh) 2017-10-16 2024-09-03 里珍纳龙药品有限公司 灌注生物反应器及相关使用方法
WO2019118921A1 (en) 2017-12-14 2019-06-20 Flodesign Sonics, Inc. Acoustic transducer drive and controller
WO2020168315A1 (en) * 2019-02-15 2020-08-20 Just-Evotec Biologics, Inc. Automated biomanufacturing systems, facilities, and processes

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US5225539A (en) 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
US5476996A (en) 1988-06-14 1995-12-19 Lidak Pharmaceuticals Human immune system in non-human animal
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
GB8823869D0 (en) 1988-10-12 1988-11-16 Medical Res Council Production of antibodies
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US6673986B1 (en) 1990-01-12 2004-01-06 Abgenix, Inc. Generation of xenogeneic antibodies
US6150584A (en) 1990-01-12 2000-11-21 Abgenix, Inc. Human antibodies derived from immunized xenomice
US6075181A (en) 1990-01-12 2000-06-13 Abgenix, Inc. Human antibodies derived from immunized xenomice
DE69133566T2 (de) 1990-01-12 2007-12-06 Amgen Fremont Inc. Bildung von xenogenen Antikörpern
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
US6172197B1 (en) 1991-07-10 2001-01-09 Medical Research Council Methods for producing members of specific binding pairs
EP0814159B1 (en) 1990-08-29 2005-07-27 GenPharm International, Inc. Transgenic mice capable of producing heterologous antibodies
US5625126A (en) 1990-08-29 1997-04-29 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
US5770429A (en) 1990-08-29 1998-06-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US6255458B1 (en) 1990-08-29 2001-07-03 Genpharm International High affinity human antibodies and human antibodies against digoxin
US5874299A (en) 1990-08-29 1999-02-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5661016A (en) 1990-08-29 1997-08-26 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
US6300129B1 (en) 1990-08-29 2001-10-09 Genpharm International Transgenic non-human animals for producing heterologous antibodies
WO1994025585A1 (en) 1993-04-26 1994-11-10 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5789650A (en) 1990-08-29 1998-08-04 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
US5814318A (en) 1990-08-29 1998-09-29 Genpharm International Inc. Transgenic non-human animals for producing heterologous antibodies
US5633425A (en) 1990-08-29 1997-05-27 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5877397A (en) 1990-08-29 1999-03-02 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
ES2227513T5 (es) 1991-10-25 2009-04-01 Immunex Corporation Citoquina novedosa.
ATE408012T1 (de) 1991-12-02 2008-09-15 Medical Res Council Herstellung von autoantikörpern auf phagenoberflächen ausgehend von antikörpersegmentbibliotheken
CA2124967C (en) 1991-12-17 2008-04-08 Nils Lonberg Transgenic non-human animals capable of producing heterologous antibodies
US5660731A (en) * 1994-11-08 1997-08-26 Pall Corporation Filter for separating photoactive agent
US5705364A (en) 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
US5721121A (en) 1995-06-06 1998-02-24 Genentech, Inc. Mammalian cell culture process for producing a tumor necrosis factor receptor immunoglobulin chimeric protein
WO1996040918A2 (en) 1995-06-07 1996-12-19 Immunex Corporation Novel cd40l mutein
KR100942863B1 (ko) 1999-08-24 2010-02-17 메다렉스, 인코포레이티드 인간 씨티엘에이-4 항체 및 그의 용도
JP3523245B1 (ja) 2000-11-30 2004-04-26 メダレックス，インコーポレーテッド ヒト抗体作製用トランスジェニック染色体導入齧歯動物
WO2002101019A2 (en) 2001-06-13 2002-12-19 Genentech, Inc. Methods of culturing animal cells and polypeptide production in animal cells
JP2007525984A (ja) 2004-03-05 2007-09-13 ディーエスエム アイピー アセッツ ビー．ブイ． 連続的灌流および交互接線流による細胞培養の方法
FR2884255B1 (fr) 2005-04-11 2010-11-05 Vivalis Utilisation de lignees de cellules souches aviaires ebx pour la production de vaccin contre la grippe
WO2008024769A2 (en) 2006-08-21 2008-02-28 Raven Biotechnologies, Inc. Intensified perfusion production method
AU2007351548B2 (en) * 2006-11-01 2012-08-16 Biogen Ma Inc. Method of isolating biomacromolecules using low pH and divalent cations
US9012180B2 (en) * 2007-03-02 2015-04-21 Wyeth Llc Use of copper and glutamate in cell culture for production of polypeptides
EP2188371B1 (en) * 2007-08-09 2017-12-20 Wyeth LLC Use of perfusion to enhance production of fed-batch cell culture in bioreactors
KR20110028272A (ko) * 2008-06-09 2011-03-17 다니스코 유에스 인크. 배양 브로쓰로부터의 불용성 효소의 회수 및 불용성 효소의 제형화
JP5746025B2 (ja) * 2008-07-16 2015-07-08 ケーセップ・システムズ，リミテッド・ライアビリティ・カンパニー 流動床を使用して粒子を操作するための方法及びシステム
US20110184154A1 (en) * 2008-10-17 2011-07-28 Percivia Llc Cell broth clarification and host cell protein removal
TW201041899A (en) * 2009-04-13 2010-12-01 Bristol Myers Squibb Co Protein purification by citrate precipitation
DK2501822T3 (da) * 2009-11-17 2017-11-27 Squibb & Sons Llc Fremgangsmåder til forbedret proteinproduktion
WO2014133741A1 (en) * 2013-02-26 2014-09-04 Emd Millipore Corporation Selective removal of a protein from a mixture of proteins using activated carbon by adjusting solution conditions

Also Published As

Publication number Publication date
WO2011062926A3 (en) 2011-07-21
DK2501822T3 (da) 2017-11-27
HRP20171701T8 (hr) 2018-02-23
EP3431608A2 (en) 2019-01-23
PL2501822T3 (pl) 2017-12-29
NO2501822T3 (hu) 2018-01-13
US20130017577A1 (en) 2013-01-17
SI2501822T1 (sl) 2017-10-30
EP3431608A3 (en) 2019-02-20
EP3255153A1 (en) 2017-12-13
ES2642629T3 (es) 2017-11-17
PT2501822T (pt) 2017-10-18
LT2501822T (lt) 2017-10-25
HRP20171701T1 (hr) 2017-12-15
CY1119923T1 (el) 2018-12-12
RS56484B1 (sr) 2018-01-31
US20140302581A1 (en) 2014-10-09
US8765415B2 (en) 2014-07-01
EP2501822A2 (en) 2012-09-26
WO2011062926A2 (en) 2011-05-26
EP2501822B1 (en) 2017-08-16

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