HU191376B - Process for producing bioaktive material of yield increasing activity from the nitrogenfixing roots of leguminosae - Google Patents

Abstract

It is proposed that the nodules are removed from the plant roots, cleaned, crushed and mixed with water (4 parts by weight of water to one part of nodule material). The mixt. is homogenised until a thick, milky material is obtained, which is then strained several times to remove foreign material and the fibrous cell-wall material of the nodules. - The suspension is mixed with a further 5 parts of water. The contents of the diluted suspension, mainly proteins, are then precipitated and separated by means of settling and centrifuging.

Description

FIELD OF THE INVENTION The present invention relates to a process for the production of a bioactive material extracted from a nitrogen-collecting glomerium of a butterfly plant, which provides a versatile beneficial effect without artificial substances.

It is generally known that plant organs, inorganic and organic materials, and combinations thereof, can be used to produce materials that will advantageously influence the quantity and quality of the crop in some of the cultivated plants. These substances promote the development, growth, development of fruiting organs, improve plant metabolism, etc.

Various materials have already been prepared for increasing the yield of plants, but it is a mistake of the known materials that they generally have a more or less high yield-increasing effect when applied to only one or two plant species and only in a certain state of development of the plant species.

A further disadvantage of known crop-enhancing substances is that, when mixed with other substances, their beneficial effect is largely lost and therefore cannot be applied to the plant together. Their application generally requires a separate operation, which makes the use of known crop enhancers considerably more expensive.

A disadvantage of known materials is that relatively large amounts of these have to be applied per hectare and are therefore environmentally harmful.

British Patent 2,105,699 discloses a process for using extracts of natural plant materials as foliar fertilizers. In this process, a dilute aqueous emulsion of the rice bran wax fraction is applied to the soil, especially under unfavorable growing conditions for rice, wheat, corn and sunflower. The process is based on the knowledge that non-saponifiable substances made mainly from vegetable waxes, such as rice bran wax, can be used as plant nutrient materials.

The disadvantage of this process is that the preparation of the rice bran fraction is very complicated and takes a long time. Many expensive chemicals are needed and expensive physical and chemical processes are required. Inevitably, large-scale production requires high-investment equipment. All of these increase the price of the product significantly and make it uneconomical to use the British process, especially under more favorable growing conditions.

U.S. Patent No. 922,104 describes a process for producing a butterfly seed graft. Essentially agricultural fertilizers are supplemented with bacterial cultures. The purpose of the procedure differs from that of the previous similar procedures in that it is intended to increase the graft titre, to reduce the amount of foreign microflora and to increase the graft storage time.

In the process, peat, earth or a mixture of the two (kills, the material contained in the bags are moistened to 35-40%, the bags are sealed and their contents are sterilized at a radiation dose of 2.5-4.0 Mrad). they are provided with energy sources for bacteria (glucose, sucrose sth.j and protective agents (glycerol, dextrin, etc.)) cl.

Liquid cultures of soybean bacteria are prepared by sub-sub-cultivation in flasks and then treated in 100 L fermentation media in a specially formulated liquid medium. This culture set is injected with a hollow needle into prepared bags as described. The puncture site is sealed, the contents of the bags are mixed with a special machine and the resulting soybean graft composition is stored at 10-14 ° C.

The disadvantage of this process is that the resulting product cannot be used as foliar fertilizer. The resulting product is a high moisture, earthy or peaty graft which cannot be distributed as evenly as liquid or lyophilized materials. The resulting graft should be stored at low temperatures, which can only be achieved through large building and machine investment.

U.S. Patent No. 787,398 describes a process for producing organic mineral fertilizers for enhancing crop yields. The essence of the process is that the known lignin hydrolysis is carried out in the presence of unicellular green algae, unlike in the prior art. The organic mineral fertilizers produced contain 98% organic mineral mass, 0.9% copper trace element and biologically active substances selected by algae during their life activity, and 1.1% moisture. The resulting mineral fertilizer is used to increase the yield of cotton.

The disadvantage of this process is that the resulting product is a very expensive, solid organic mineral fertilizer in which the bioactive material presumed by the algae is present only as an adjuvant. Not suitable for foliar fertilization.

SUMMARY OF THE INVENTION It is an object of the present invention to provide a method of producing a crop-growing bioactive material from a nitrogen-collecting tuber of butterflies, which can be used to increase or improve the yield and quality of various crops such as wheat, corn, and soybeans. can be combined with herbicides, fungicides and without the need for special work, and which has a versatile effect, which increases germination vigor, providing fast and even germination, stimulates the growth of the crop early , shade the soil and therefore reduce the drying of the soil surface and inhibit weeds, favorably affect the development of crop-forming organs it provides the basis for higher yields, enhances photosynthetic activity and thus mobilizes nutrient uptake, which affects eyelids, pods, etc. and thus generates a significant increase of 1000 seeds, which is an important crop component, eliminates the need to use a substantial amount of fertilizer, which is both economically and environmentally significant, and can be produced with little investment, in relatively short working hours and cheaply.

SUMMARY OF THE INVENTION The present invention is directed to a process for the production of bioactive materials derived from the nitrogen-collecting guineas of butterfly plants, characterized in that the roots of the butterfly plants are separated from the roots, the leaves, the roots and the roots, first cleaned dry, followed by continuous washing, crushed from the cleaned tubers, add 1 part by weight of tuber to 4 parts by weight of water and mix, for example, until homogeneous until a finely divided, dense cream is obtained. foreign matter and

The fibrous cell wall of -2191,376 tubers was removed, and the resulting suspension was diluted with an additional 5 parts by weight of water, and the predominantly protein-containing compounds in the diluted suspension were precipitated by superficial centrifugation.

A further feature of the process of the present invention is the germination of a yellowish clear liquid remaining after removal of the predominantly proteinaceous compounds which forms a solution of the bioactive yield enhancing agent.

The production of the bioactive substance according to the invention has led to the discovery that the nitrogen-harvesting extracts of the butterfly flowering plants produced by the process of the present invention have a yield-increasing effect when applied during the early stages of plant development or during flowering, vaginal, spray on the plant stock. Experiments with the bioactive substance produced by the process have also shown that the yield-enhancing effect is also present in important arable crops such as wheat and maize, although they do not enter into symbiosis with nitrogen-binding Rhizobium.

In the process, the bulbs are first detached from the roots of the butterfly plants and cleaned of all contaminants and soil in conventional cleaning and washing machines. The pure tubers are crushed and crushed. The crushing is done on a machine of known type.

Thereafter, 4 parts by weight of water are added to the crumbs of each part by weight and they are moved in a mixer until a finely divided, dense and homogeneous cream is obtained. Once this has been achieved, the material is passed through appropriate filters several times to filter out any foreign matter and remnants of the fibrous cell wall of the mime. An additional 5 parts by weight of water is added to the resulting slurry, homogenized by stirring or the like, and the resulting diluted suspension is predominantly proteinaceous and is removed by centrifugation. The remaining yellowish clear liquid is germinated before use.

As described above, the bioactive substance of the present invention can be applied to a variety of plants and at various stages of plant growth, but the magnitude and intensity of the effect will naturally depend on the particular plant and its phenophase sprayed to the plant. Experiments were carried out to examine the intensity of Writing, the results of which were tabulated.

Table 1 shows the evolution of emergence percentages for different soybean varieties. In this experimental cultivation, according to the method of the present invention, the soybean biocompatible material was applied to the soil immediately after sowing and it was found that the germination power increased and, as a result, the number of viable plants emerged. As can be seen from Table 1, on the 12th day after sowing, with the same number of experimental and control sowing, the average of the breeding stock was 68%, that of the treated and treated stock was much higher 18 %. In the post-emergence period, the treated plants developed faster, earlier shading the soil and thus reducing the evaporation of the soil surface and suppressing the weeds.

The bioactive material produced by the process of the present invention can also be successfully used in wheat and corn cultivation, since it mainly promotes the formation of fruiting organs such as corn and tube and thus provides the basis for higher yields.

Λ Bioactive material can be successfully sprayed on the plant even during seed dressing, seeding, and legging because it enhances the intensity of photosynthesis, accelerates the biological maturation process and increases the 1000 seed mass, which is a significant yield increasing component.

Λ The versatile action of a bioactive substance allows the treatment to be carried out in the optimum manner in one or more stages of development according to the specific genetic and physiological characteristics of the particular plant. For example, in wheat, during our experiments and field trials, we found two developmental states where the treatment proved to be most effective. The ego is a state of abrasion, the other is the time of flowering-milking. The results of these experiments are shown in Figures 2-5. Tables show.

According to our experiments, spraying the bioactive substance on the crop at the beginning of the tassel digestion yields the highest yield. The results obtained with the treatment of maize are shown in Table 6

At his word, at the beginning of vaginal filling, treatment with the peak of the cluster is probably the most effective. The results of experiments on soy are shown in Table 7.

The process of the present invention provides 2000 ml of bioacrylic stock solution from 200 g of crude tuber which develops and is separated from soybean roots. The bioactive substance can be sprayed on the plants by itself, dissolved in 300-400 liters of water per hectare, but can also be sprayed with herbicides and fungicides. Spraying can be done using standard tank techniques.

The most important advantageous properties of the process according to the invention are as follows:

The process can be done inexpensively with standard machines and implements. The biocompatible material increases the germination ability, stimulates growth and thus early shadows and inhibits weeds. It stimulates the development of fruiting organs, enhances the activity of photosynthesis and thus increases the seed weight of 1000. It can be combined with and sprayed with other plant growth regulators, herbicides, fungicides. It reduces the use of fertilizers and thus the pollution of the environment.

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Table 1

Effect of Soya Güi Female Extract on Soybean Keii Strength (% Rise 6 and 12 Days After Sowing)

Soybean kind Rising% On the 6th day after sowing On the 12th day after sowing control handled control handled Ewans 46 86 60 88 ISP. 16 70 80 85 87 S 1346 35 54 60 70 Average: 50 70 68 81

Table 2

Results of a wheat leaf spray experiment with soybean extract (Regszemcse I., MV 4 wheat varieties)

Control Treated 1000 seed weights

Treatment time Yield, kg / ha Arányszúrn Yield, kg / ha golden Eye Control, g Treated, g Shrubbing IV. 6th '5100 100 6550 128.4 44,00 48.50 Origin of the IV. 20th 5250 100 5800 110.4 42.25 47,50 Flowering VI. First 5050 100 6000 118.8 42.50 50.00 Φ 112.2 42.31 48.66

Table 3

Yields of wheat Icing spray experiment on wheat with soybean extract

Time of treatment control Handled 1000 seed weights Yield, kg / ha Proportion Yield, kg / ha ratio Control, g Treated, g Origin of the IV. 20th 5000 100 5220 104.4 44.0 50.0 Flowering VI. First Φ 5050 100 6330 125.3 114.8 42.0 43.5 50.2 50.1

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Table 4

Results of a wheat leaf spray experiment with soybean extract

Time of treatment control Handled 1000 seeds weight Yield, kg / ha ratio Yield, kg / ha ratio Control, g Treatment, g tillering ARC. 5th 5200 100 6220 119.6 34.4 36.4 run-up IV: 16. 5500 100 5880 106.9 35.8 38.4 ear protection V. 27. 5650 100 6000 106.1 35.2 38.6 Flowering VI. 9th 5530 100 5000 106.6 36.8 37.8 Φ 109.8 35.5 37.8

Table 5

Results of a wheat leaf spraying experiment with soya glyme extract (Bicsárd, II., GK Szeged variety)

Control Treated 1000 seed weights

Time of treatment Yield, kg / ha ratio Yield, kg / ha ratio Control, g Treatment, g Shrubbing IV. 5th 5070 100 6120 120.7 37.6 38.8 Origin of the IV. 16th 5550 100 5870 105.6 34.8 36.0 Piracy V. 27. 5600 100 6370 113.7 35.8 37.6 Flowering VI. 9th 5300 100 6350 119.8 36.2 37.8 Φ 114.0 36.1 37.5

Table 6

Results of a corn leaf extraction experiment with Soya glyme extract (Regszemcse-Bicsér)

Treatment Regszemcse Bicsérd Yield, t / ha ratio Yield, t / ha ratio control 7.29 100.0 10.72 100.0 At the beginning of coat of arms 8.25 1 13.2 12.03 112.2

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ISP. 16 soy varieties

Ewans soy variety

Table 7

Yield results of a soybean leaf sprayer experiment with soy glyme extract

Treatment Untreated Sprayed Untreated Sprayed D i t p ο n tj a_____________

crop kg / ha Gold- song crop kg / ha Gold- song crop kg / ha Gold song crop kg / ha Gold- song V. 13. 2930 100.0 3280 111.9 3000 100.0 3300 110.0 VI. 15th 2900 100.0 2950 101.7 3050 100.0 3200 104.9 VI.28. 2950 100.0 3250 110.2 2700 100.0 3300 122.2

Claims

Claims (2)

CLAIMS 1. A process for the production of a bioactive substance extracted from nitrogen-collecting tubers of butterfly plants, comprising removing the tuber from the roots of the butterfly plants, drying the tuber off the stems, leaves, roots and then dry purify the tubers by crushing, adding 1 part by weight of gluten meal to 4 parts by weight of water, and homogenizing the resulting mixture until a fine, dense cream is obtained, filtering off any foreign matter from the resulting material and removing the fibrous cell wall of the tubers. after dilution with additional 5 parts by weight of water, the predominantly protein-containing compounds in the diluted suspension are precipitated by centrifugation to settle. 2. A process as claimed in claim 1, wherein the yellow liquid θθ, which remains after the removal of predominantly proteinaceous compounds, is germinated as a solution of a bioactive yield enhancing agent.