HRP20231266T1 - Novi biopesticidi za suzbijanje biljnih štetnika - Google Patents
Novi biopesticidi za suzbijanje biljnih štetnika Download PDFInfo
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- HRP20231266T1 HRP20231266T1 HRP20231266TT HRP20231266T HRP20231266T1 HR P20231266 T1 HRP20231266 T1 HR P20231266T1 HR P20231266T T HRP20231266T T HR P20231266TT HR P20231266 T HRP20231266 T HR P20231266T HR P20231266 T1 HRP20231266 T1 HR P20231266T1
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- Prior art keywords
- molecules
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- pleurotolysin
- seq
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Links
- 241000607479 Yersinia pestis Species 0.000 title claims 6
- 230000000853 biopesticidal effect Effects 0.000 title 1
- 241000196324 Embryophyta Species 0.000 claims 13
- 125000003275 alpha amino acid group Chemical group 0.000 claims 12
- 229920001184 polypeptide Polymers 0.000 claims 12
- 102000004196 processed proteins & peptides Human genes 0.000 claims 12
- 108090000765 processed proteins & peptides Proteins 0.000 claims 12
- 238000000034 method Methods 0.000 claims 7
- 108010043683 ostreolysin Proteins 0.000 claims 6
- 108090000623 proteins and genes Proteins 0.000 claims 6
- 102000004169 proteins and genes Human genes 0.000 claims 6
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 4
- 241000238631 Hexapoda Species 0.000 claims 4
- 230000009261 transgenic effect Effects 0.000 claims 4
- 241000258916 Leptinotarsa decemlineata Species 0.000 claims 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
- 241000254173 Coleoptera Species 0.000 claims 1
- 241000489975 Diabrotica Species 0.000 claims 1
- 241000489947 Diabrotica virgifera virgifera Species 0.000 claims 1
- 241001057636 Dracaena deremensis Species 0.000 claims 1
- 241000258915 Leptinotarsa Species 0.000 claims 1
- 244000061456 Solanum tuberosum Species 0.000 claims 1
- 235000002595 Solanum tuberosum Nutrition 0.000 claims 1
- 240000008042 Zea mays Species 0.000 claims 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims 1
- 108010089166 aegerolysin Proteins 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 235000005822 corn Nutrition 0.000 claims 1
- 244000038559 crop plants Species 0.000 claims 1
- 244000038280 herbivores Species 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 239000011814 protection agent Substances 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/375—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Basidiomycetes
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Insects & Arthropods (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
- Catching Or Destruction (AREA)
Claims (15)
1. Upotreba dvokomponentnog proteinskog kompleksa koji se sastoji od mnoštva molekula člana obitelji egerolizina odabranih iz skupine koju čine ostreolizin i pleurotolizin A, i mnoštva molekula člana super obitelji MACPF koji je pleurotolizin B (PlyB) za suzbijanje biljnih nametnika;
pri čemu je ostreolizin polipeptid koji sadrži aminokiselinsku sekvencu koja ima najmanje 90% identičnosti sekvence sa SEQ ID NO: 1;
pri čemu je pleurotolizin A polipeptid koji sadrži aminokiselinsku sekvencu koja ima najmanje 90% identičnosti sekvence sa SEQ ID NO: 2; i
pri čemu je pleurotolizin B polipeptid koji sadrži aminokiselinsku sekvencu koja ima najmanje 90% identičnosti sekvence sa SEQ ID NO: 4.
2. Upotreba prema zahtjevu 1, naznačena time što se dvokomponentni proteinski kompleks sastoji od 20 do 30 molekula članova obitelji aegerolizina i 10 do 15 molekula članova super obitelji MACPF.
3. Upotreba prema zahtjevu 1 ili 2, naznačena time što omjer molekula članova obitelji egerolizina prema molekulama članovima super obitelji MACPF iznosi 2:1.
4. Upotreba prema bilo kojem od zahtjeva 1 do 3, pri naznačena time što se dvokomponentni proteinski kompleks sastoji od 20 molekula članova obitelji egerolizina i 10 molekula članova super obitelji MACPF, ili sastoji se od 22 molekula članova obitelji egerolizina i 11 molekula članova super obitelji MACPF, ili sastoji se od 24 molekula članova obitelji egerolizina i 12 molekula članova super obitelji MACPF, ili sastoji se od 26 molekula članova obitelji egerolizina i 13 molekula članova super obitelji MACPF, ili sastoji se od 28 molekula članova obitelji egerolizina i 14 molekula članova super obitelji MACPF, ili sastoji se od 30 molekula članova obitelji egerolizina i 15 molekula članova super obitelji MACPF.
5. Upotreba prema bilo kojem od zahtjeva 1 do 4, pri čemu je ostreolizin polipeptid koji sadrži aminokiselinsku sekvencu koja ima najmanje 90%, kao što je najmanje 95%, najmanje 97% ili najmanje 99% identičnosti sekvence sa SEQ ID NO: 1; pri čemu je pleurotolizin A polipeptid koji sadrži aminokiselinsku sekvencu koja ima najmanje 90%, kao što je najmanje 95%, najmanje 97% ili najmanje 99% identičnosti sekvence sa SEQ ID NO: 2; i pri čemu pleurotolizin B je polipeptid koji sadrži aminokiselinsku sekvencu koja ima najmanje 90%, kao što je najmanje 95%, najmanje 97% ili najmanje 99% identičnosti sekvence sa SEQ ID NO: 4.
6. Postupak za zaštitu biljke od biljnih nametnika ili za suzbijanje biljnih nametnika, koji se sastoji od koraka: primjene na biljku kojoj je to potrebno pripravka koji sadrži mnoštvo molekula članova obitelji egerolizina odabranih iz skupine koju čine ostreolizin i pleurotolizin A, mnoštvo molekula člana super obitelji MACPF koji je pleurotolizin B (PlyB) i prikladnog nosača, kao što je puferska otopina;
pri čemu je ostreolizin polipeptid koji sadrži aminokiselinsku sekvencu koja ima najmanje 90% identičnosti sekvence sa SEQ ID NO: 1;
pri čemu je pleurotolizin A polipeptid koji sadrži aminokiselinsku sekvencu koja ima najmanje 90% identičnosti sekvence sa SEQ ID NO: 2; i
pri čemu pleurotolizin B je polipeptid koji sadrži aminokiselinsku sekvencu koja ima najmanje 90% identičnosti sekvence sa SEQ ID NO: 4.
7. Postupak prema zahtjevu 6, naznačen time što je ostreolizin polipeptid koji sadrži aminokiselinsku sekvencu koja ima najmanje 90%, kao što je najmanje 95%, najmanje 97% ili najmanje 99% identičnosti sekvence sa SEQ ID NO: 1; pri čemu je pleurotolizin A polipeptid koji sadrži aminokiselinsku sekvencu koja ima najmanje 90%, kao što je najmanje 95%, najmanje 97% ili najmanje 99% identičnosti sekvence sa SEQ ID NO: 2; i pri čemu je pleurotolizin B polipeptid koji sadrži aminokiselinsku sekvencu koja ima najmanje 90%, kao što je najmanje 95%, najmanje 97% ili najmanje 99% identičnosti sekvence sa SEQ ID NO: 4.
8. Postupak prema zahtjevu 6 ili 7, naznačena time što je molarni omjer između članova obitelji egerolizina i članova superobitelji MACPF u rasponu od oko 3:1 do oko 1000:1, kao što je oko 5:1, oko 10:1, oko 20:1, oko 25:1, oko 30:1, oko 40:1, oko 50:1, oko 60:1, oko 70:1, oko 80:1, oko 90:1, oko 100:1, oko 200:1, oko 300:1, oko 400:1, oko 500:1, oko 600:1, oko 700:1, oko 800:1, ili oko 900:1, ili oko 1000:1.
9. Upotreba prema bilo kojem od zahtjeva 1 do 5 ili postupak prema bilo kojem od zahtjeva 6 do 8, naznačena time što je biljni štetnik kukac.
10. Upotreba prema bilo kojem od zahtjeva 1 do 5 ili postupak prema bilo kojem od zahtjeva 6 do 8, naznačena time što je biljni štetnik insekt biljožder.
11. Upotreba ili postupak prema zahtjevu 9 ili 10, naznačena time što je kukac iz roda Leptinotarsa ili Diabrotica.
12. Upotreba ili postupak prema zahtjevu 9 ili 10, naznačena time što je kukac odabran iz skupine koju čine Leptinotarsa decemlineata (koloradska krumpirova zlatica) i Diabrotica virgifera virgifera (kukuruzne zlatice).
13. Upotreba dvokomponentnog proteinskog kompleksa kako je definiran u bilo kojem od zahtjeva 1 do 5 ili pripravka kako je definiran u bilo kojem od zahtjeva 6 do 8, naznačena time što se koristi za pripravu sredstva za zaštitu bilja.
14. Transgena biljka ili njezino potomstvo naznačena time što eksprimira ili je sposobna eksprimirati dvokomponentni proteinski kompleks kako je definirano u bilo kojem od zahtjeva 1 do 5; navedena transgena biljka ili njezino potomstvo sadrži jednu ili više rekombinantnih molekula nukleinske kiseline koje sadrže nukleotidne sekvence koje kodiraju navedeni dvokomponentni proteinski kompleks, a navedene nukleotidne sekvence su operativno povezane s najmanje jednim promotorom koji je funkcionalan u navedenoj biljnoj stanici tako da uzrokuje proizvodnju mRNA molekula.
15. Transgena biljka ili njezino potomstvo prema zahtjevu 14, naznačena time da je navedena transgena biljka ili njezino potomstvo biljka usjeva, kao što je biljka krumpira ili kukuruza.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2017/074877 WO2019063101A1 (en) | 2017-09-29 | 2017-09-29 | NEW BIOPESTICIDES TO CONTROL PLANT PESTS |
| EP17784590.6A EP3662069B1 (en) | 2017-09-29 | 2017-09-29 | New bio-pesticides for controlling plant pests |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20231266T1 true HRP20231266T1 (hr) | 2024-02-02 |
Family
ID=60117646
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20231266TT HRP20231266T1 (hr) | 2017-09-29 | 2017-09-29 | Novi biopesticidi za suzbijanje biljnih štetnika |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20200245628A1 (hr) |
| EP (1) | EP3662069B1 (hr) |
| CN (1) | CN111164212A (hr) |
| BR (1) | BR112020005331A2 (hr) |
| CA (1) | CA3077263A1 (hr) |
| ES (1) | ES2963455T3 (hr) |
| HR (1) | HRP20231266T1 (hr) |
| PL (1) | PL3662069T3 (hr) |
| WO (1) | WO2019063101A1 (hr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HRP20231266T1 (hr) * | 2017-09-29 | 2024-02-02 | Kmetijski Institut Slovenije | Novi biopesticidi za suzbijanje biljnih štetnika |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1794308B1 (en) * | 2004-09-29 | 2013-08-28 | Pioneer-Hi-Bred International, Inc. | Corn event das-59122-7 and methods for detection thereof |
| HRP20231266T1 (hr) * | 2017-09-29 | 2024-02-02 | Kmetijski Institut Slovenije | Novi biopesticidi za suzbijanje biljnih štetnika |
-
2017
- 2017-09-29 HR HRP20231266TT patent/HRP20231266T1/hr unknown
- 2017-09-29 CN CN201780095414.9A patent/CN111164212A/zh active Pending
- 2017-09-29 PL PL17784590.6T patent/PL3662069T3/pl unknown
- 2017-09-29 WO PCT/EP2017/074877 patent/WO2019063101A1/en unknown
- 2017-09-29 ES ES17784590T patent/ES2963455T3/es active Active
- 2017-09-29 US US16/649,385 patent/US20200245628A1/en active Pending
- 2017-09-29 CA CA3077263A patent/CA3077263A1/en active Pending
- 2017-09-29 EP EP17784590.6A patent/EP3662069B1/en active Active
- 2017-09-29 BR BR112020005331-7A patent/BR112020005331A2/pt active Search and Examination
Also Published As
| Publication number | Publication date |
|---|---|
| PL3662069T3 (pl) | 2024-03-11 |
| ES2963455T3 (es) | 2024-03-27 |
| US20200245628A1 (en) | 2020-08-06 |
| BR112020005331A2 (pt) | 2020-09-24 |
| CN111164212A (zh) | 2020-05-15 |
| EP3662069A1 (en) | 2020-06-10 |
| EP3662069C0 (en) | 2023-08-30 |
| CA3077263A1 (en) | 2019-04-04 |
| WO2019063101A1 (en) | 2019-04-04 |
| EP3662069B1 (en) | 2023-08-30 |
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