HRP20210513T1 - Postupak fermentacije s poboljšanom pretvorbom glicerola i octene kiseline - Google Patents
Postupak fermentacije s poboljšanom pretvorbom glicerola i octene kiseline Download PDFInfo
- Publication number
- HRP20210513T1 HRP20210513T1 HRP20210513TT HRP20210513T HRP20210513T1 HR P20210513 T1 HRP20210513 T1 HR P20210513T1 HR P20210513T T HRP20210513T T HR P20210513TT HR P20210513 T HRP20210513 T HR P20210513T HR P20210513 T1 HRP20210513 T1 HR P20210513T1
- Authority
- HR
- Croatia
- Prior art keywords
- glycerol
- reactor
- yeast cell
- nucleotide sequences
- sequences encoding
- Prior art date
Links
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 title claims 24
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 title claims 12
- 238000000855 fermentation Methods 0.000 title claims 6
- 230000004151 fermentation Effects 0.000 title claims 6
- 238000006243 chemical reaction Methods 0.000 title 1
- 238000000034 method Methods 0.000 claims 17
- 210000005253 yeast cell Anatomy 0.000 claims 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 6
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 5
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims 2
- 238000002955 isolation Methods 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 102000008146 Acetate-CoA ligase Human genes 0.000 claims 1
- 108010049926 Acetate-CoA ligase Proteins 0.000 claims 1
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
- 108010041921 Glycerolphosphate Dehydrogenase Proteins 0.000 claims 1
- 108010015895 Glycerone kinase Proteins 0.000 claims 1
- BAWFJGJZGIEFAR-NNYOXOHSSA-O NAD(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-O 0.000 claims 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims 1
- 102000003673 Symporters Human genes 0.000 claims 1
- 108090000088 Symporters Proteins 0.000 claims 1
- 230000021736 acetylation Effects 0.000 claims 1
- 238000006640 acetylation reaction Methods 0.000 claims 1
- 108010081577 aldehyde dehydrogenase (NAD(P)+) Proteins 0.000 claims 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims 1
- 238000012217 deletion Methods 0.000 claims 1
- 230000037430 deletion Effects 0.000 claims 1
- 230000001419 dependent effect Effects 0.000 claims 1
- 238000007865 diluting Methods 0.000 claims 1
- 239000008103 glucose Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 150000007524 organic acids Chemical class 0.000 claims 1
- 239000002994 raw material Substances 0.000 claims 1
- AWUCVROLDVIAJX-GSVOUGTGSA-N sn-glycerol 3-phosphate Chemical compound OC[C@@H](O)COP(O)(O)=O AWUCVROLDVIAJX-GSVOUGTGSA-N 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C31/00—Saturated compounds having hydroxy or O-metal groups bound to acyclic carbon atoms
- C07C31/02—Monohydroxylic acyclic alcohols
- C07C31/08—Ethanol
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01006—Glycerol dehydrogenase (1.1.1.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
- C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
- C12Y207/01028—Triokinase (2.7.1.28)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
- C12Y207/01029—Glycerone kinase (2.7.1.29), i.e. dihydroxyacetone kinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
- C12Y602/01—Acid-Thiol Ligases (6.2.1)
- C12Y602/01001—Acetate-CoA ligase (6.2.1.1)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Claims (14)
1. Postupak za proizvodnju etanola koji obuhvaća fermentaciju lignoceluloznog hidrolizata u šaržno napajanom reaktoru u šaržnom načinu rada sa stanicom kvasca, koja se sposobna za pretvorbu šećera, glicerola i octene kiseline, koji obuhvaća sljedeće korake:
a) inokuliranje razrijeđenog lignoceluloznog hidrolizata sa stanicom kvasca;
c) dodavanje lignoceluloznog hidrolizata koji sadrži glicerol u reaktor;
d) nakon dovoljnog vremena fermentacije, izoliranje etanola iz reaktora,
e) po izboru zadržavanje preostale frakcije nakon izolacije iz koraka d) kao istrošene juhe; i
f) po izboru korištenje istrošene juhe u koraku a) za razrjeđivanje lignoceluloznog hidrolizata, pri čemu se pH smjese u šaržno napajanom reaktoru u šaržnom načinu rada održava konstantnim dodavanjem dovoljne količine lignoceluloznog hidrolizata.
2. Postupak u skladu s patentnim zahtjevom 1, naznačen time što korak c) obuhvaća postupno dodavanje u reaktor lignoceluloznog hidrolizata dopunjenog glicerolom.
3. Postupak u skladu s patentnim zahtjevom 1 ili zahtjevom 2, naznačen time što je dodana količina glicerola takva da je molarna koncentracija glicerola u reaktoru dvostruko veća od molarne koncentracije ili 1,8 do 2,2 puta veća od molarne koncentracije octene kiseline u reaktoru.
4. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 3, naznačen time što dodavanje glicerola započinje kada je koncentracija glukoze u reaktoru 2 g/l ili niža.
5. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 4, naznačen time što je stanica kvasca sposobna trošiti ksilozu u lignoceluloznom hidrolizatu.
6. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 5, naznačen time što je stanica kvasca genetski modificirana, koja obuhvaća:
a) jednu ili više nukleotidnih sekvenci koje kodiraju heterolognu NAD+- ovisnu acetilacijsku acetaldehid dehidrogenazu (E.C. 1.2.1.10);
b) jednu ili više nukleotidnih sekvenci koje kodiraju homolognu ili heterolognu acetil-CoA sintetazu (E.C. 6.2.1.1);
c) jednu ili više nukleotidnih sekvenci koje kodiraju heterolognu glicerol dehidrogenazu (E.C. 1.1.1.6); i
d) jednu ili više nukleotidnih sekvenci koje kodiraju homolognu ili heterolognu dihidroksiaceton kinazu (E.C. 2.7.1.28 ili E.C. 2.7.1.29).
7. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 6, naznačen time što stanica kvasca ima deleciju ili poremećaj jedne ili više endogenih nukleotidnih sekvenci koje kodiraju glicerol 3-fosfat fosfohidrolazu i/ili kodiraju gen glicerol 3-fosfat dehidrogenaze.
8. Postupak u skladu s patentnim zahtjevom 6 ili 7, naznačen time što stanica kvasca eksprimira egzogeni simporter glicerola.
9. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 8, naznačen time što se tijekom postupka u smjesu u reaktoru ne dodaje baza.
10. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 9, naznačen time što je stanica kvasca sposobna metabolizirati organsku kiselinu.
11. Postupak u skladu s patentnim zahtjevom 10, naznačen time što je stanica kvasca sposobna metabolizirati octenu kiselinu.
12. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 11, naznačen time što je postupak anaerobni ili anoksični šaržni postupak fermentacije u kojem je pH fermentacijske juhe u reaktoru viši od pH dovedene sirovine.
13. Postupak u skladu s bilo kojim od patentnih zahtjeva 7 to 12, naznačen time što stanica kvasca može anaerobno fermentirati najmanje jedan C6 šećer i najmanje jedan C5 šećer.
14. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 13, naznačen time što se postupku fermentacije dodaje postupak razmnožavanja.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14199434 | 2014-12-19 | ||
| EP15817235.3A EP3234161B1 (en) | 2014-12-19 | 2015-12-17 | Fermentation process with improved glycerol and acetic acid conversion |
| PCT/EP2015/080330 WO2016097202A1 (en) | 2014-12-19 | 2015-12-17 | Fermentation process with improved glycerol and acetic acid conversion |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20210513T1 true HRP20210513T1 (hr) | 2021-05-14 |
Family
ID=52354690
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20210513TT HRP20210513T1 (hr) | 2014-12-19 | 2021-03-30 | Postupak fermentacije s poboljšanom pretvorbom glicerola i octene kiseline |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US10883122B2 (hr) |
| EP (2) | EP3831950A1 (hr) |
| CN (1) | CN107109441A (hr) |
| BR (1) | BR112017012624A2 (hr) |
| CA (1) | CA2969162C (hr) |
| DK (1) | DK3234161T3 (hr) |
| ES (1) | ES2864715T3 (hr) |
| HR (1) | HRP20210513T1 (hr) |
| HU (1) | HUE053810T2 (hr) |
| MY (1) | MY188314A (hr) |
| PL (1) | PL3234161T3 (hr) |
| WO (1) | WO2016097202A1 (hr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL3234161T3 (pl) * | 2014-12-19 | 2021-10-25 | Dsm Ip Assets B.V. | Proces fermentacji z ulepszoną konwersją glicerolu i kwasu octowego |
| EP3559220A1 (en) * | 2016-12-23 | 2019-10-30 | DSM IP Assets B.V. | Improved glycerol free ethanol production |
| CA3074001A1 (en) * | 2017-09-26 | 2019-04-04 | Dsm Ip Assets B.V. | Acetic acid consuming strain |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2601052T3 (es) * | 2006-10-02 | 2017-02-14 | Dsm Ip Assets B.V. | Ingeniería metabólica de células de levadura que fermentan arabinosa |
| WO2011149353A1 (en) * | 2010-05-27 | 2011-12-01 | C5 Yeast Company B.V. | Yeast strains engineered to produce ethanol from acetic acid and glycerol |
| CA2834053C (en) | 2010-11-18 | 2019-03-26 | Dsm Ip Assets B.V. | Yeast strains engineered to produce ethanol from glycerol |
| AU2012346662B2 (en) * | 2011-11-30 | 2016-07-28 | Danisco Us Inc. | Yeast strains engineered to produce ethanol from acetic acid and glycerol |
| WO2014072232A1 (en) | 2012-11-07 | 2014-05-15 | Dsm Ip Assets B.V. | pH CONTROLLED YEAST PROPAGATION |
| WO2014180820A2 (en) * | 2013-05-08 | 2014-11-13 | Dsm Ip Assets B.V. | Gpd- yeast strains with improved osmotolerance |
| AR097479A1 (es) * | 2013-08-29 | 2016-03-16 | Dsm Ip Assets Bv | Células convertidoras de glicerol y ácido acético con un transporte de glicerol mejorado |
| AR097480A1 (es) | 2013-08-29 | 2016-03-16 | Dsm Ip Assets Bv | Células de levadura convertidoras de glicerol y ácido acético con una conversión de ácido acético mejorada |
| PL3234161T3 (pl) * | 2014-12-19 | 2021-10-25 | Dsm Ip Assets B.V. | Proces fermentacji z ulepszoną konwersją glicerolu i kwasu octowego |
-
2015
- 2015-12-17 PL PL15817235T patent/PL3234161T3/pl unknown
- 2015-12-17 EP EP20207094.2A patent/EP3831950A1/en active Pending
- 2015-12-17 BR BR112017012624A patent/BR112017012624A2/pt not_active Application Discontinuation
- 2015-12-17 WO PCT/EP2015/080330 patent/WO2016097202A1/en active Application Filing
- 2015-12-17 HU HUE15817235A patent/HUE053810T2/hu unknown
- 2015-12-17 MY MYPI2017701719A patent/MY188314A/en unknown
- 2015-12-17 EP EP15817235.3A patent/EP3234161B1/en active Active
- 2015-12-17 DK DK15817235.3T patent/DK3234161T3/da active
- 2015-12-17 CA CA2969162A patent/CA2969162C/en active Active
- 2015-12-17 CN CN201580069126.7A patent/CN107109441A/zh active Pending
- 2015-12-17 ES ES15817235T patent/ES2864715T3/es active Active
- 2015-12-17 US US15/535,961 patent/US10883122B2/en active Active
-
2020
- 2020-11-12 US US17/096,751 patent/US11667935B2/en active Active
-
2021
- 2021-03-30 HR HRP20210513TT patent/HRP20210513T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP3831950A1 (en) | 2021-06-09 |
| MY188314A (en) | 2021-11-28 |
| EP3234161A1 (en) | 2017-10-25 |
| CA2969162A1 (en) | 2016-06-23 |
| ES2864715T3 (es) | 2021-10-14 |
| CN107109441A (zh) | 2017-08-29 |
| CA2969162C (en) | 2023-09-26 |
| US10883122B2 (en) | 2021-01-05 |
| HUE053810T2 (hu) | 2021-07-28 |
| DK3234161T3 (da) | 2021-05-31 |
| EP3234161B1 (en) | 2021-03-17 |
| PL3234161T3 (pl) | 2021-10-25 |
| US20170335350A1 (en) | 2017-11-23 |
| US20210071205A1 (en) | 2021-03-11 |
| BR112017012624A2 (pt) | 2018-04-10 |
| US11667935B2 (en) | 2023-06-06 |
| WO2016097202A1 (en) | 2016-06-23 |
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