HRP20201442T1 - Poboljšano rekombinantno izražavanje proteina korištenjem hibridnog promotora chef1 - Google Patents
Poboljšano rekombinantno izražavanje proteina korištenjem hibridnog promotora chef1 Download PDFInfo
- Publication number
- HRP20201442T1 HRP20201442T1 HRP20201442TT HRP20201442T HRP20201442T1 HR P20201442 T1 HRP20201442 T1 HR P20201442T1 HR P20201442T T HRP20201442T T HR P20201442TT HR P20201442 T HRP20201442 T HR P20201442T HR P20201442 T1 HRP20201442 T1 HR P20201442T1
- Authority
- HR
- Croatia
- Prior art keywords
- expression vector
- host cell
- cell
- promoter
- hybrid
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title 1
- 210000004027 cell Anatomy 0.000 claims 9
- 239000013604 expression vector Substances 0.000 claims 7
- 241000701022 Cytomegalovirus Species 0.000 claims 4
- 241000699802 Cricetulus griseus Species 0.000 claims 2
- 230000001105 regulatory effect Effects 0.000 claims 2
- 241000699800 Cricetinae Species 0.000 claims 1
- 102000010292 Peptide Elongation Factor 1 Human genes 0.000 claims 1
- 108010077524 Peptide Elongation Factor 1 Proteins 0.000 claims 1
- 210000003527 eukaryotic cell Anatomy 0.000 claims 1
- 210000005260 human cell Anatomy 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 210000001672 ovary Anatomy 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000002103 transcriptional effect Effects 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361777603P | 2013-03-12 | 2013-03-12 | |
| EP14727377.5A EP2971005B1 (en) | 2013-03-12 | 2014-03-11 | Improved recombinant protein expression using a hybrid chef1 promoter |
| PCT/US2014/023661 WO2014164869A1 (en) | 2013-03-12 | 2014-03-11 | Improved recombinant protein expression using a hybrid chef1 promoter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20201442T1 true HRP20201442T1 (hr) | 2020-12-11 |
Family
ID=50846904
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20201442TT HRP20201442T1 (hr) | 2013-03-12 | 2020-09-10 | Poboljšano rekombinantno izražavanje proteina korištenjem hibridnog promotora chef1 |
Country Status (18)
| Country | Link |
|---|---|
| US (4) | US9297024B2 (zh) |
| EP (2) | EP2971005B1 (zh) |
| KR (1) | KR102365484B1 (zh) |
| CN (2) | CN105209625B (zh) |
| AU (1) | AU2014248936B2 (zh) |
| CA (1) | CA2904125C (zh) |
| CY (1) | CY1123336T1 (zh) |
| DK (1) | DK2971005T3 (zh) |
| ES (1) | ES2811648T3 (zh) |
| HK (1) | HK1220491A1 (zh) |
| HR (1) | HRP20201442T1 (zh) |
| HU (1) | HUE050362T2 (zh) |
| LT (1) | LT2971005T (zh) |
| NZ (1) | NZ711654A (zh) |
| PL (1) | PL2971005T3 (zh) |
| PT (1) | PT2971005T (zh) |
| SI (1) | SI2971005T1 (zh) |
| WO (1) | WO2014164869A1 (zh) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2904125C (en) * | 2013-03-12 | 2022-06-07 | Cmc Icos Biologics, Inc. | Improved recombinant protein expression using a hybrid chef1 promoter |
| AR095196A1 (es) | 2013-03-15 | 2015-09-30 | Regeneron Pharma | Medio de cultivo celular libre de suero |
| EP3356543A4 (en) | 2015-09-28 | 2019-05-29 | The Trustees of Columbia University in the City of New York | EXPRESSION SYSTEM FOR MODIFIED BACTERIAL PROTEINS |
| GB201603577D0 (en) | 2016-03-01 | 2016-04-13 | Oxford Genetics Ltd | Promoter |
| CN107043773B (zh) * | 2017-02-08 | 2018-09-18 | 四川丰讯科技发展有限公司 | 一种提高哺乳动物细胞中外源基因表达水平的基因片段、重组载体及其用途 |
| WO2019028273A1 (en) * | 2017-08-02 | 2019-02-07 | Dna2.0, Inc. Dba Atum | DNA VECTORS AND ELEMENTS FOR SUSTAINED GENE EXPRESSION IN EUKARYOTIC CELLS |
| AU2019206443A1 (en) * | 2018-01-10 | 2020-07-23 | Cmc Icos Biologics, Inc. | Bidirectional CHEFl vectors |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5888809A (en) | 1997-05-01 | 1999-03-30 | Icos Corporation | Hamster EF-1α transcriptional regulatory DNA |
| EP1492874B1 (en) * | 2002-03-29 | 2011-01-19 | XOMA Technology Ltd. | Multigenic vector plasmids and methods for increasing expression of recombinant polypeptides |
| KR100533794B1 (ko) * | 2003-10-02 | 2005-12-07 | 주식회사 프로젠 | 인간 난포자극 호르몬을 대량으로 생산하는 방법 |
| CA2590284A1 (en) * | 2004-12-08 | 2006-06-15 | Icos Corporation | Recombinant method for making multimeric proteins |
| PL1969127T5 (pl) * | 2005-12-21 | 2018-02-28 | Cnj Holdings, Inc | Metoda wytwarzania biologicznie czynnych białek zależnych od witaminy K metodami rekombinacji |
| WO2007081336A1 (en) * | 2006-01-13 | 2007-07-19 | Five Prime Therapeutics, Inc. | Mammalian vectors for high-level expression of recombinant proteins |
| EP2035572A4 (en) * | 2006-06-30 | 2010-01-06 | Univ Michigan | PROCESS FOR THE PREPARATION OF FACTOR VIII PROTEINS BY RECOMBINATION PROCEDURES |
| NZ598275A (en) * | 2009-08-06 | 2014-11-28 | Cmc Icos Biolog Inc | Methods for improving recombinant protein expression |
| WO2012052878A1 (en) * | 2010-10-19 | 2012-04-26 | Vascular Biogenics Ltd. | Isolated polynucleotides and nucleic acid constructs for directing expression of a gene-of-interest in cells |
| CA2904125C (en) * | 2013-03-12 | 2022-06-07 | Cmc Icos Biologics, Inc. | Improved recombinant protein expression using a hybrid chef1 promoter |
-
2014
- 2014-03-11 CA CA2904125A patent/CA2904125C/en active Active
- 2014-03-11 KR KR1020157027697A patent/KR102365484B1/ko active IP Right Grant
- 2014-03-11 AU AU2014248936A patent/AU2014248936B2/en active Active
- 2014-03-11 WO PCT/US2014/023661 patent/WO2014164869A1/en active Application Filing
- 2014-03-11 PL PL14727377T patent/PL2971005T3/pl unknown
- 2014-03-11 LT LTEP14727377.5T patent/LT2971005T/lt unknown
- 2014-03-11 US US14/204,879 patent/US9297024B2/en active Active
- 2014-03-11 HU HUE14727377A patent/HUE050362T2/hu unknown
- 2014-03-11 PT PT147273775T patent/PT2971005T/pt unknown
- 2014-03-11 DK DK14727377.5T patent/DK2971005T3/da active
- 2014-03-11 ES ES14727377T patent/ES2811648T3/es active Active
- 2014-03-11 EP EP14727377.5A patent/EP2971005B1/en active Active
- 2014-03-11 EP EP20180248.5A patent/EP3770263A1/en not_active Withdrawn
- 2014-03-11 SI SI201431654T patent/SI2971005T1/sl unknown
- 2014-03-11 CN CN201480014593.5A patent/CN105209625B/zh active Active
- 2014-03-11 NZ NZ711654A patent/NZ711654A/en unknown
- 2014-03-11 CN CN201910162032.4A patent/CN110042122A/zh active Pending
-
2016
- 2016-03-07 US US15/062,982 patent/US9957525B2/en active Active
- 2016-07-19 HK HK16108568.7A patent/HK1220491A1/zh unknown
-
2018
- 2018-03-29 US US15/940,282 patent/US20190071691A1/en not_active Abandoned
-
2020
- 2020-09-08 CY CY20201100847T patent/CY1123336T1/el unknown
- 2020-09-10 HR HRP20201442TT patent/HRP20201442T1/hr unknown
-
2021
- 2021-01-06 US US17/142,971 patent/US20210340560A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20210340560A1 (en) | 2021-11-04 |
| US20160177335A1 (en) | 2016-06-23 |
| KR20150126399A (ko) | 2015-11-11 |
| PT2971005T (pt) | 2020-09-01 |
| US9957525B2 (en) | 2018-05-01 |
| SI2971005T1 (sl) | 2020-10-30 |
| US9297024B2 (en) | 2016-03-29 |
| ES2811648T3 (es) | 2021-03-12 |
| AU2014248936A1 (en) | 2015-09-24 |
| PL2971005T3 (pl) | 2020-12-14 |
| EP3770263A1 (en) | 2021-01-27 |
| DK2971005T3 (da) | 2020-08-24 |
| LT2971005T (lt) | 2020-10-12 |
| NZ711654A (en) | 2021-07-30 |
| HUE050362T2 (hu) | 2020-11-30 |
| US20190071691A1 (en) | 2019-03-07 |
| CN110042122A (zh) | 2019-07-23 |
| CN105209625A (zh) | 2015-12-30 |
| CA2904125A1 (en) | 2014-10-09 |
| CY1123336T1 (el) | 2021-12-31 |
| EP2971005B1 (en) | 2020-06-17 |
| US20140273208A1 (en) | 2014-09-18 |
| KR102365484B1 (ko) | 2022-02-18 |
| AU2014248936B2 (en) | 2020-07-09 |
| EP2971005A1 (en) | 2016-01-20 |
| CA2904125C (en) | 2022-06-07 |
| CN105209625B (zh) | 2019-03-29 |
| WO2014164869A1 (en) | 2014-10-09 |
| HK1220491A1 (zh) | 2017-05-05 |
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