HK1253945A1 - 麥芽糖依賴性降解決定子、麥芽糖響應型啟動子、穩定化構建體及其在生成非分解代謝化合物中的用途 - Google Patents

麥芽糖依賴性降解決定子、麥芽糖響應型啟動子、穩定化構建體及其在生成非分解代謝化合物中的用途

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Publication number
HK1253945A1
HK1253945A1 HK18113095.7A HK18113095A HK1253945A1 HK 1253945 A1 HK1253945 A1 HK 1253945A1 HK 18113095 A HK18113095 A HK 18113095A HK 1253945 A1 HK1253945 A1 HK 1253945A1
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Hong Kong
Prior art keywords
maltose
production
responsive promoters
catabolic compounds
dependent degrons
Prior art date
Application number
HK18113095.7A
Other languages
English (en)
Inventor
Penelope R Chua
Hanxiao Jiang
Adam Leon Meadows
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Amyris Inc
Total Marketing Services
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Publication date
Application filed by Amyris Inc, Total Marketing Services filed Critical Amyris Inc
Publication of HK1253945A1 publication Critical patent/HK1253945A1/zh

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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12P19/44Preparation of O-glycosides, e.g. glucosides
    • C12P19/60Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
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HK18113095.7A 2015-06-25 2018-10-12 麥芽糖依賴性降解決定子、麥芽糖響應型啟動子、穩定化構建體及其在生成非分解代謝化合物中的用途 HK1253945A1 (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562184793P 2015-06-25 2015-06-25
US201562266436P 2015-12-11 2015-12-11
PCT/US2016/039386 WO2016210343A1 (en) 2015-06-25 2016-06-24 Maltose dependent degrons, maltose-responsive promoters, stabilization constructs, and their use in production of non-catabolic compounds

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HK1253945A1 true HK1253945A1 (zh) 2019-07-05

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HK18113095.7A HK1253945A1 (zh) 2015-06-25 2018-10-12 麥芽糖依賴性降解決定子、麥芽糖響應型啟動子、穩定化構建體及其在生成非分解代謝化合物中的用途
HK18113115.3A HK1254052A1 (zh) 2015-06-25 2018-10-12 麥芽糖依賴性降解決定子、麥芽糖響應型啟動子、穩定化構建體及其在生成非分解代謝化合物中的用途

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US (4) US10808015B2 (zh)
EP (2) EP3313996A1 (zh)
CN (2) CN108474008A (zh)
AU (2) AU2016284696B2 (zh)
BR (2) BR112017027869A2 (zh)
HK (2) HK1253945A1 (zh)
WO (2) WO2016210343A1 (zh)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3663392A1 (en) * 2013-08-07 2020-06-10 Amyris, Inc. Methods for stabilizing production of acetyl-coenzyme a derived compounds
CN108474008A (zh) 2015-06-25 2018-08-31 阿迈瑞斯公司 麦芽糖依赖性降解决定子、麦芽糖响应型启动子、稳定化构建体及其在生成非分解代谢化合物中的用途
US11446398B2 (en) 2016-04-11 2022-09-20 Obsidian Therapeutics, Inc. Regulated biocircuit systems
PT3574105T (pt) 2017-01-25 2021-05-31 Amyris Bio Products Portugal Unipessoal Lda Coprodução de um sesquiterpeno e um carotenoide
US11629340B2 (en) 2017-03-03 2023-04-18 Obsidian Therapeutics, Inc. DHFR tunable protein regulation
EP3381932A1 (en) 2017-03-28 2018-10-03 Technische Universität Berlin Modified mussel proteins, uses thereof and related compounds
EP3720944A1 (en) 2017-12-07 2020-10-14 Zymergen Inc. Engineered biosynthetic pathways for production of (6e)-8-hydroxygeraniol by fermentation
CN111868047A (zh) 2017-12-21 2020-10-30 齐默尔根公司 荆芥内半缩醛氧化还原酶、荆芥内半缩醛合酶和能够产生荆芥内酯的微生物
WO2019241315A1 (en) 2018-06-12 2019-12-19 Obsidian Therapeutics, Inc. Pde5 derived regulatory constructs and methods of use in immunotherapy
US20210386788A1 (en) 2018-10-24 2021-12-16 Obsidian Therapeutics, Inc. Er tunable protein regulation
US10519475B1 (en) 2018-11-21 2019-12-31 Amyris, Inc. Biosynthesis of compounds in yeast
CN110592131B (zh) * 2018-12-14 2021-10-22 中国科学院天津工业生物技术研究所 麦芽糖转录激活因子MalR的突变体文库构建筛选及其应用
CN110592080B (zh) * 2018-12-17 2021-11-23 中国科学院天津工业生物技术研究所 优化的麦芽糖启动子突变体及其应用
WO2020185632A1 (en) 2019-03-08 2020-09-17 Obsidian Therapeutics, Inc. Human carbonic anhydrase 2 compositions and methods for tunable regulation
US20220127620A1 (en) 2019-03-15 2022-04-28 Andrew P. Klein Microbial production of compounds
EP3983538A1 (en) 2019-06-12 2022-04-20 Obsidian Therapeutics, Inc. Ca2 compositions and methods for tunable regulation
WO2020252404A1 (en) 2019-06-12 2020-12-17 Obsidian Therapeutics, Inc. Ca2 compositions and methods for tunable regulation
CN114302958A (zh) * 2019-06-24 2022-04-08 丹尼斯科美国公司 用于增加发酵联产物中赖氨酸含量的经修饰的酵母和方法
US20220282226A1 (en) 2019-08-01 2022-09-08 Amyris, Inc. Modified host cells for high efficiency production of vanillin
US20220348937A1 (en) 2019-09-06 2022-11-03 Obsidian Therapeutics, Inc. Compositions and methods for dhfr tunable protein regulation
CN110878288B (zh) * 2019-12-09 2020-12-29 安徽农业大学 多肽、核酸及其在合成橙花叔醇糖苷上的应用
BR112023003204A2 (pt) 2020-09-15 2023-03-28 Amyris Inc Composições de cultura e métodos de seu uso para produção de alto rendimento de vanilina
EP4308713A1 (en) 2021-03-19 2024-01-24 Amyris, Inc. Modified host cells for high efficiency production of vanillin
WO2023230411A1 (en) 2022-05-26 2023-11-30 Amyris, Inc. Improved oligosaccharide production in yeast
WO2024064888A2 (en) * 2022-09-23 2024-03-28 North Carolina State University Engineered microorganisms with enhanced protein expression and secretion
CN115813869A (zh) * 2022-12-15 2023-03-21 深圳市奥极因科技有限公司 一种感知进食状态智能给药的重组菌含片

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3477936D1 (en) 1983-03-08 1989-06-01 Merck & Co Inc Recombinant dna cloning vector pve1, deletion and hybrid mutants, and recombinant derivatives thereof, products and processes
US4703009A (en) 1983-03-08 1987-10-27 Merck & Co., Inc. RDNA cloning vector pVE1, deletion and hybrid mutants and recombinant derivatives thereof products and processes
US5272065A (en) 1983-10-20 1993-12-21 Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
US5672497A (en) 1986-03-21 1997-09-30 Eli Lilly And Company Method for increasing the antibiotic-producing ability of antibiotic-producing microorganisms
CA1340599C (en) 1986-03-21 1999-06-22 Karen Leigh Cox Antibiotic-producing microorganisms
US5098837A (en) 1988-06-07 1992-03-24 Eli Lilly And Company Macrolide biosynthetic genes for use in streptomyces and other organisms
US5149638A (en) 1988-09-29 1992-09-22 Eli Lilly And Company Tylosin biosynthetic genes tylA, tylB and tylI
US5252474A (en) 1989-03-31 1993-10-12 Merck & Co., Inc. Cloning genes from Streptomyces avermitilis for avermectin biosynthesis and the methods for their use
WO1993013663A1 (en) 1992-01-17 1993-07-22 Abbott Laboratories Method of directing biosynthesis of specific polyketides
US5824513A (en) 1991-01-17 1998-10-20 Abbott Laboratories Recombinant DNA method for producing erythromycin analogs
US6060234A (en) 1991-01-17 2000-05-09 Abbott Laboratories Polyketide derivatives and recombinant methods for making same
EP0726940A4 (en) 1993-11-02 2001-05-23 Merck & Co Inc DNA ENCODING A TRIOL-POLYCETIDE-SYNTHASE
US5716849A (en) 1994-06-08 1998-02-10 Novartis Finance Corporation Genes for the biosynthesis of soraphen
US5824545A (en) 1995-11-01 1998-10-20 Millennium Pharaceuticals, Inc. Identification of eukaryotic growth-related genes and promoter isolation vector and method of use
CA2197160C (en) 1996-02-22 2007-05-01 Stanley Gene Burgett Platenolide synthase gene
CA2197524A1 (en) 1996-02-22 1997-08-22 Bradley Stuart Dehoff Polyketide synthase genes
WO1998007868A1 (en) 1996-08-20 1998-02-26 Novartis Ag Rifamycin biosynthesis gene cluster
WO1999005283A2 (fr) 1997-07-25 1999-02-04 Hoechst Marion Roussel Genes de biosynthese et de transfert des 6-desoxyhexoses chez saccharopolyspora erythraea et chez streptomyces antibioticus et leur utilisation
US6090601A (en) 1998-01-23 2000-07-18 Kosan Bioscience Sorangium polyketide synthase
US6143526A (en) 1998-03-09 2000-11-07 Baltz; Richard H. Biosynthetic genes for spinosyn insecticide production
CA2328427A1 (en) 1998-05-28 1999-12-02 Kosan Biosciences, Inc. Recombinant narbonolide polyketide synthase
NZ508326A (en) 1998-06-18 2003-10-31 Novartis Ag A polyketide synthase and non ribosomal peptide synthase genes, isolated from a myxobacterium, necessary for synthesis of epothiones A and B
US6265202B1 (en) 1998-06-26 2001-07-24 Regents Of The University Of Minnesota DNA encoding methymycin and pikromycin
IL142165A0 (en) 1998-10-02 2002-03-10 Kosan Biosciences Inc Polyketide synthase enzymes and recombinant dna constructs therefor
JP2003512013A (ja) 1998-10-29 2003-04-02 コーサン バイオサイエンシーズ, インコーポレイテッド 組換えオレアンドライドポリケチドシンターゼ
AU768220B2 (en) 1998-11-20 2003-12-04 Kosan Biosciences, Inc. Recombinant methods and materials for producing epothilone and epothilone derivatives
AU7332301A (en) * 2000-07-19 2002-02-05 Advanced Res & Tech Inst Novel fibroblast growth factor (FGF23) and methods for use
EP1379549A2 (fr) * 2001-02-07 2004-01-14 Institut Pasteur Sequence du genome de photorhabdus luminescens souche tto1 et utilisations
US7192751B2 (en) 2001-12-06 2007-03-20 The Regents Of The University Of California Biosynthesis of amorpha-4,11-diene
GB0402660D0 (en) * 2004-02-06 2004-03-10 Medical Res Council Heterologous protein expression
US20060141570A1 (en) * 2004-11-16 2006-06-29 Wood David W Intein-mediated protein purification using in vivo expression of an aggregator protein
CN101443354A (zh) * 2006-04-14 2009-05-27 新英格兰生物实验室公司 与突变麦芽糖结合蛋白融合的靶蛋白的溶解和纯化
US8623615B2 (en) * 2009-04-01 2014-01-07 New England Biolabs, Inc. Solubilization and purification of a target protein fused to a mutant maltose-binding protein
JP6158170B2 (ja) 2011-04-27 2017-07-12 アミリス, インコーポレイテッド ゲノム修飾のための方法
CA2879178C (en) * 2012-08-07 2020-11-24 Amyris, Inc. Methods for stabilizing production of acetyl-coenzyme a derived compounds
EP3663392A1 (en) 2013-08-07 2020-06-10 Amyris, Inc. Methods for stabilizing production of acetyl-coenzyme a derived compounds
CN108474008A (zh) * 2015-06-25 2018-08-31 阿迈瑞斯公司 麦芽糖依赖性降解决定子、麦芽糖响应型启动子、稳定化构建体及其在生成非分解代谢化合物中的用途

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AU2016284689A1 (en) 2018-01-25
US20210230234A1 (en) 2021-07-29
AU2016284696A1 (en) 2018-01-25
EP3313995B1 (en) 2023-06-07
US20180171341A1 (en) 2018-06-21
AU2016284689B9 (en) 2022-01-20
AU2016284696B2 (en) 2021-10-28
US10808015B2 (en) 2020-10-20
US20200399326A1 (en) 2020-12-24
US20180186841A1 (en) 2018-07-05
US10988513B2 (en) 2021-04-27
HK1254052A1 (zh) 2019-07-12
BR112017027970A2 (pt) 2018-08-28
EP3313995A1 (en) 2018-05-02
EP3313996A1 (en) 2018-05-02
EP3313995C0 (en) 2023-06-07
CN108474009A (zh) 2018-08-31
WO2016210343A1 (en) 2016-12-29
BR112017027869A2 (pt) 2018-09-11
CN108474008A (zh) 2018-08-31
CN108474009B (zh) 2022-10-04
WO2016210350A1 (en) 2016-12-29
AU2016284689B2 (en) 2021-10-07

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