HK1195259B - Cosmetic composition containing green tea component - Google Patents

Abstract

The present invention provides a cosmetic composition containing one or more selected from a group consisting of green tea saponin and green tea polyphenol as active ingredients. The cosmetic composition of the invention is very safe to the skin and can greatly enhance biological mechanisms in the skin, and thus can serve as an anti-aging cosmetic composition.

Description

Cosmetic composition containing green tea component

Technical Field

The present invention relates to a cosmetic composition containing a green tea ingredient.

Background

Recently, with the vigorous development of plant cell culture techniques utilizing totipotency of plants, attention to functional secondary metabolites derived from plants has been increased greatly. It is known that functional secondary metabolites have various physiological activities, and thus substances extracted from plant stem cells are attracting attention as new raw materials for highly functional cosmetics. Since this technology enables the production of economical highly functional active substances, which are naturally occurring but difficult to obtain by chemical synthesis, it is expected that new components, which have never been used, can be developed and applied. In addition, material development of bioprocesses such as using the active transformation technology (the active transformation technology) by applying enzyme treatment is also performed in a compact drum. Although the demand for functional cosmetics has recently been increasing, the development of new functional materials or related technologies has not yet been able to satisfy the demand. In this article, cosmetics prepared using plant stem cell technology or biological processes such as enzyme treatment, which are capable of preparing many new functional ingredients, are attracting attention for their various skin effects, particularly their anti-aging effects.

Disclosure of Invention

Technical problem

The present inventors have studied to develop a cosmetic containing natural plant components that do not cause adverse reactions to the skin and are capable of providing excellent anti-aging and anti-wrinkle effects, and have found that green tea components can provide such effects.

The present invention relates to providing a cosmetic composition capable of exhibiting excellent skin stability and excellent skin anti-aging and anti-wrinkle effects by using plant-derived ingredients.

Technical scheme

In general, the present invention provides a cosmetic composition containing one or more substances selected from the group consisting of green tea saponin and green tea polyphenol as an active ingredient.

In an exemplary embodiment of the present invention, the composition may further comprise a green tea stem cell culture product.

In an exemplary embodiment of the present invention, the green tea saponin may be a substance extracted from green tea seed peel and from which sugar residues are removed.

In an exemplary embodiment of the invention, the green tea polyphenol may be Epigallocatechin gallate (EGCG) extracted from green tea leaves.

In an exemplary embodiment of the invention, the amount of green tea saponin or green tea polyphenol may be 0.001-1 wt% of the total weight of the composition, respectively.

In an exemplary embodiment of the invention, the stem cells may be callus (callus) -derived stem cells.

In an exemplary embodiment of the present invention, the stem cell culture product may be one or more substances selected from the group consisting of stem cell strains, lysates thereof, extracts thereof, and culture solutions thereof.

In an exemplary embodiment of the present invention, the stem cell culture product may be present in an amount of 0.01 to 10 wt% based on the total weight of the composition.

In one exemplary embodiment of the present invention, the composition may be an anti-aging composition.

In one exemplary embodiment of the present invention, the composition may be a composition for moisturizing skin or enhancing skin barrier function.

In an exemplary embodiment of the invention, the composition may activate the filaggrin gene (filaggrin gene).

In one exemplary embodiment of the present invention, the composition may be a composition for whitening skin or inhibiting skin pigmentation.

In an exemplary embodiment of the invention, the composition can activate the interleukin 6(IL-6) gene.

In one exemplary embodiment of the present invention, the composition may be a composition for enhancing skin elasticity or improving skin wrinkles.

In an exemplary embodiment of the invention, the composition can activate a catalase gene.

Advantageous effects

The cosmetic composition of the present invention can be used as an anti-aging cosmetic composition because the composition can exhibit excellent skin stability and provide excellent skin improvement biological mechanisms, such as activating genes. In addition, since the green tea ingredient of the present invention can promote the gene activity decreased by aging again, increasing the biosynthesis of collagen in a concentration manner, the green tea ingredient can fundamentally improve aged skin, and also provide collagen to revitalize skin having lost elasticity and luster, which has not only the effect of preventing skin aging but also the effect of improving skin elasticity.

Disclosure of Invention

Hereinafter, the present invention will be described in detail.

Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art.

The present invention provides a cosmetic composition containing one or more substances selected from the group consisting of green tea saponin and green tea polyphenol as an active ingredient. The composition may further comprise a culture product of stem cells of green tea (camellia sinensis).

The green tea saponin of the present invention can be a substance obtained by extracting a high-molecular-weight crude saponin from green tea seed husk and removing a sugar residue with an enzyme, and thus can be easily absorbed by the skin and exhibit high efficiency.

The green tea polyphenol of the present invention may be epigallocatechin gallate (EGCG), which is enriched in green tea leaves, is a powder obtained by extracting green tea leaves with warm water and solidifying the product.

The green tea saponin or green tea polyphenol can be 0.001-1 wt%, preferably 0.01-0.5 wt% of the total weight of the composition. If the content of each component is less than 0.001 wt%, skin cell regeneration and antioxidant effects cannot be expected. Further, if the content exceeds 1.0 wt%, the effectiveness of the ingredients may be reduced because the effect is not further improved. In addition, various solvents are combined in order to stably contain poorly soluble green tea saponin and polyphenol in a dosage form, thereby reducing the value as a cosmetic composition.

The green tea stem cells can be obtained by using a recently valued plant stem cell culture technique. A stem cell line is established by culturing totipotent callus derived from seeds, leaves, stems, roots, etc. of green tea by solid cell culture, and active ingredients are produced in large quantities therefrom by suspension cell culture, followed by extraction.

The culture product may be selected from the group consisting of the induced stem cell strain itself, a lysate thereof, an extract thereof, and a culture solution thereof. The extract is not particularly limited by the extraction method, and can be obtained by extraction by, for example, a method of culturing a cell line derived from a tissue mass of a plant. In preparation example 1 of the present invention, a stem cell culture solution obtained from leaf-derived callus and a lysate of the stem cell line can be used. However, the same results as in the present invention can also be obtained by a stem cell line, lysate, extract or culture solution thereof obtained by culturing callus derived from embryo, cambium or procambium of green tea.

The culture product may contain, for example, an amino acid isolated/purified from green tea as an active ingredient. The extract of the present invention can fundamentally combat skin aging by increasing again the activity of major genes, which decrease with skin aging. Further, there is provided an effect of increasing collagen synthesis in the skin to increase elastic fibers in the dermis to improve or reduce wrinkles. In addition, it can eliminate active oxygen clusters which are the main cause of skin aging. Therefore, an excellent anti-aging effect is exhibited by solving the decrease in gene activity, which is the root cause of skin aging, and isolating oxidative stress.

The amount of the stem cell culture product is 0.01-10 wt%, preferably 0.1-5 wt% based on the total weight of the composition. If the content is less than 0.01 wt%, an anti-aging effect by gene reactivation cannot be expected. Further, if the content exceeds 10 wt%, the effectiveness of the ingredients will be reduced because the effect is not further improved.

The cosmetic composition of the present invention may be an anti-aging composition.

Further, the cosmetic composition of the present invention may be a composition for moisturizing skin, enhancing skin barrier function, whitening skin, inhibiting skin pigmentation, enhancing skin elasticity, resisting oxidation, or improving skin wrinkles.

Human skin contains three types of cells. They are keratinocytes, which make up most of the epidermis, melanocytes, which produce melanin, and fibroblasts, which make up most of the dermis. Keratinocytes are associated with moisturizing by preventing water loss and barrier function to protect the skin from harmful factors. Melanocytes can determine skin color and tone, and are also the cause of freckles and blemishes. Fibroblasts are capable of producing elastic fibers, such as collagen, and are associated with skin elasticity and skin wrinkles. According to studies on skin genetics, the decrease in activity in the three cells is most evident with increasing annual population: the filaggrin gene of keratinocytes, the interleukin 6(IL-6) gene of melanocytes, and the catalase gene of fibroblasts. Namely, these three genes are closely related to skin aging.

Filaggrin is a differentiation marker for keratinocytes and is known as a precursor of natural moisturizing factors. Interleukin 6 is a low melanogenesis factor and is known to inhibit melanogenesis in melanocytes. Catalase is an enzyme that catalyzes the decomposition of peroxide produced in cells.

The present invention is expected to bring a rejuvenation effect to aged skin by activating these three genes.

The composition of the invention is capable of activating the filaggrin gene. If the filaggrin gene is activated, the moisturizing effect of inhibiting the evaporation of water from the skin can be enhanced, and the skin barrier effect for protecting the skin from external harmful factors can be enhanced.

The compositions of the invention are capable of activating the interleukin 6(IL-6) gene. If the IL-6 gene is activated, the skin whitening effect is improved due to the lightening of skin color and tone, and the skin pigmentation can be suppressed due to the reduction of freckles and blemishes.

The composition of the present invention is capable of activating a catalase gene. If the catalase gene is activated, collagen synthesis can be increased, however, skin elasticity and skin wrinkles can be improved.

The composition of the present invention is not particularly limited by the dosage form. For example, it may be a base cosmetic, a makeup cosmetic, a hair care cosmetic, a body care cosmetic, etc., and may be appropriately selected according to the purpose.

The cosmetic composition may be formulated, for example, as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleanser, an oil, a powder foundation, an emulsion foundation, a wax foundation, a spray, etc., but is not limited thereto. More preferably, it can be made into basic cosmetics such as skin softening lotion, nourishing lotion, emulsion, body lotion, nourishing cream, massage cream, moisturizing cream, hand cream, essence, eye cream, cleansing foam, cleansing lotion, pack, gel, patch, oil-in-water (O/W) emulsion, water-in-oil (W/O) emulsion, etc., color cosmetics such as lipstick, pre-makeup emulsion, foundation, etc., cleansing agent such as hair lotion, hair conditioner, bath lotion, toothpaste, mouth wash, etc., or hair care cosmetics such as hair tonic, hair setting agent such as gel or mousse, hair growth promoter, hair dye, etc.

The cosmetic composition may comprise a cosmetically acceptable medium or base, and may provide any form of topical administration, such as a solution, gel, anhydrous solid or paste, oil-in-water emulsion, suspension, microemulsion, microcapsule, microparticle or ionic (liposome) type and/or nonionic vesicle dispersion, cream, lotion, emulsion, powder, ointment, spray or concealer stick. These compositions can be prepared according to methods commonly used in the art.

When the dosage form of the present invention is a solution or emulsion, a solvent, a dissolving agent or an emulsifier may be used as a carrier. For example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylene glycol, glycerol fatty acid esters, polyethylene glycol, or fatty acid esters of sorbitan may be used.

When the dosage form of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohols, polyoxyethylene sorbitol esters and polyoxyethylene sorbitol esters, microcrystalline cellulose, meta-aluminate, bentonite, agar or tragacanth may be used as carriers.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, or the like can be used as a carrier.

When the formulation of the present invention is a powder or a spray, lactose, talc, silicon dioxide, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier. In particular, when the dosage form is a spray, it may further comprise a propellant, such as chlorofluorocarbon (chlorofluorohydrocarbon), propane/butane or dimethyl ether.

When the formulation of the present invention is surfactant-containing, fatty alcohol sulfate, fatty alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazoline derivative, methyl taurine, sarcosinate, fatty acid amide ether sulfate, alkylamide betaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, ethoxylated glycerin fatty acid ester, or the like can be used as a carrier.

The cosmetic composition of the present invention may further comprise a thickener. The thickener contained in the cosmetic composition of the present invention may be methylcellulose, carboxymethylcellulose, carboxymethyl-hydroxy-guanine, hydroxymethyl cellulose, hydroxyethyl cellulose, carbopol, polyquaternary ammonium salt, cetostearyl alcohol, stearic acid, carrageenan (carrageenan), or the like. Preferably, one or more thickeners selected from the group consisting of carboxymethyl cellulose, carbopol, and polyquaternary ammonium salts may be used. More preferably, carbopol may be used.

In an exemplary embodiment of the present invention, the cosmetic composition may include various bases and additives as appropriate, and the kind and content thereof may be easily determined by those skilled in the art. The cosmetic composition may contain acceptable additives such as preservatives, colorants, additives and the like commonly used in the art. Preferably, phenoxyethanol, 1, 2-hexanediol, etc. can be used as the preservative, and a synthetic perfume can be used.

Further, the cosmetic composition of the present invention may comprise a substance selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polypeptides, polysaccharides, sphingolipids and seaweed extracts. In addition, it may further comprise oils, fats, moisturizers, emollients, surfactants, organic or inorganic pigments, organic powders, ultraviolet absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, colorants, fragrances, blood circulation stimulants, coolants, antiperspirants, purified water, and the like.

However, the ingredients contained in the cosmetic composition may not be limited thereto. Moreover, the content of the components may be determined within a range not affecting the object and effect of the present invention.

Hereinafter, the present invention will be described in detail by examples. However, it will be apparent to those of ordinary skill in the art that the following examples are for illustrative purposes only and do not limit the scope of the present invention.

[ PREPARATION EXAMPLE 1 ] extraction of active ingredient

Callus was induced by shearing green tea leaves and culturing in callus induction medium (table 1). Callus was induced starting after 15 days and callus layers were isolated starting after 30 days. After the callus layer was separated, white soft parts exhibiting good growth rate were re-cultured every 21 days with a new medium identical to the induction medium. The callus induction medium composition is shown in table 1. Auxin as a growth regulator is added into the culture medium at the concentration of 1-3 mg/L. The culture was carried out in a dark room thermostated at 25. + -. 1 ℃.

The callus was cultured on a solid medium (Guchefa), and then a growth-stable stem cell line was selected therefrom. The solid medium used in the present invention is MS medium, which has high contents of NO3-N, NH4-N and K compared to other media. The composition of the solid medium is shown in Table 2.

Selected stem cell lines were cultured in suspension medium (table 3) containing sugars and growth hormone. The composition of the suspension medium used to culture the stem cell lines is shown in Table 3.

Obtaining a mixture of the active ingredients from the cells cultured in the suspension medium. In addition, the cell wall of the cultured cells is disrupted to further obtain the components released therefrom.

Green tea saponin is obtained by extracting the seed coat of green tea, and then treating with enzyme. Repeating the process of extracting green tea leaves with warm water to obtain green tea polyphenols, and concentrating. Finally, high purity EGCG was obtained.

[ test example 1 ] Effect of promoting collagen Synthesis

Human fibroblasts were cultured in 24-well plates and then replaced with culture media containing the extraction mixture extracted from the green tea stem cells at a concentration of 10 ppm (see example 1), 5 ppm (see example 2), 1 ppm (see example 3) or 0 ppm (comparative example 1), respectively. By the third day, 0.5 mL of DMEM medium (Dulbecco's modified Eagle's medium) containing 10% fetal bovine serum was added to each well, and then 10. mu. Ci of l- [2,3,4,5-³H]-proline. After 24 hours, the medium and cells were collected from each well and washed with a 5% trichloroacetic acid (TCA) solution. The test tubes of comparative example 1 were stored at 4 ℃ while the reference examples and examples containing phenylpropanoids (phenylpropanoids) at different concentrations were incubated at 37 ℃ for 90 minutes after adding collagenase type 1 unit/. mu. L I. Subsequently, after 0.05 mL of 50% trichloroacetic acid was added to all the test tubes, the tubes were allowed to stand at 4 ℃ for 20 minutes and then centrifuged at 12,000 rpm for 10 minutes. Using Liquid Scintillation Counters (LSC)) Counts Per Minute (CPM) were determined for each supernatant and pellet, and then Relative Collagen Biosynthesis (RCB) values of reference example and comparative example 1 were calculated according to equation 1. The results are shown in Table 4.

[ EQUATION 1 ]

RCB (%) = (collagen CPM)/[ (total collagen CPM-collagen CPM) x 5.4 + collagen CPM ] x 100

As can be seen from table 4, the extract mixture extracted from green tea stem cells increased collagen biosynthesis by fibroblasts in a concentration-dependent manner.

Table 5 shows the results of determining the Relative Collagen Biosynthesis (RCB) of green tea saponin (example 1), green tea polyphenol (example 2), a mixture of green tea stem cells and green tea saponin (example 3), a mixture of green tea stem cells and green tea polyphenol (example 4), a mixture of green tea saponin and green tea polyphenol (example 5), and a mixture of green tea stem cells, green tea saponin, and green tea polyphenol (example 6).

[ test example 2 ] Gene-activating Effect

To analyze the effects of green tea stem cells, green tea saponin, and green tea polyphenol in skin cells, Normal Human Keratinocytes (NHK) and Normal Human Fibroblasts (NHF) can be used. The fibroblast may be a fibroblast taken from a replicative senescence model in which cell aging is induced by repeated culturing (sub-culturing).

For gene detection, keratinocytes may be selected for filaggrin and interleukin 6, while fibroblasts may be selected for catalase.

After cytotoxicity testing for each well, green tea stem cells, green tea saponin and green tea polyphenol were detected at the following concentrations capable of exhibiting cell viability of 80% or more. Treatment with green tea stem cells, green tea saponin and green tea polyphenol, the cells were collected for 24 hours and washed twice with 10 mL Phosphate Buffered Saline (PBS). Then, whole RNA was isolated from the cells using TRIzol reagent (Invitrogen, Carlsbad, Calif., USA). The isolated RNA was purified again using Qiagen RNeasy kit (Qiagen, Valencia, Calif.) and cDNA was synthesized therefrom using standard Reverse Transcriptase (RT) II kit (Invitrogen, Carlsbad, Calif.). Then, the expression changes of the filaggrin, interleukin 6 and catalase genes were quantitatively analyzed by real-time reverse transcription-polymerase chain reaction (Q-RT-PCR). Evaluating the change of the gene expression pattern by using a TaqMan gene expression detection kit. The primers used were: the poly keratin microfilament protein is Hs00856927_ g 1; interleukin 6 is Hs00174360_ m 1; and catalase is Hs 0015608 _ m 1. Table 6 shows the expression amounts of filaggrin, interleukin 6, and catalase of the cells analyzed by real-time PCR.

As shown in table 6, the green tea stem cell culture product (example 4), green tea saponin (example 5) and green tea EGCG (example 6) increased the expression level of the genes. In particular, the green tea stem cells increase the expression level of the interleukin 6 gene, the green tea saponin increases the expression level of the catalase gene, and the green tea EGCG is effective in increasing the expression level of the filaggrin gene. When these ingredients are used in combination (examples 7 to 10), that is, when two or more ingredients are combined, a synergistic effect can be obtained, and when these three ingredients are combined at the same time, an optimal anti-aging effect can be obtained at a gene level (example 10). The expression levels described in table 6 are shown graphically in figure 1.

[ test example 3 ] skin stability test

In order to evaluate the anti-aging cosmetic composition of the present invention, the degree of skin irritation of dosage form example 2 was measured.

The test was done at dermatology hospital, the university of north loyalty. Dosage form the cosmetic composition of example 2 was applied to the backs of 30 healthy adults, average age 33.2 years, for 48 hours, and skin reactions were monitored for 24 hours. The degree of skin irritation was assessed according to the CTFA guidelines (1981) and the Frosch & Kligman evaluation criteria.

No skin irritation was observed in 30 adults to which the cosmetic composition of the present invention (formulation example 2) was applied. It is thus understood that the cosmetic composition of the present invention has very good skin stability.

The present invention will be explained in detail by way of dosage form examples below. However, it will be apparent to those of ordinary skill in the art that the following dosage form examples are for illustrative purposes only and do not limit the scope of the present invention.

Formulation example 1 skin softening lotion (skin lotion)

Skin softening lotions were prepared according to the usual method with the compositions described in table 7.

Formulation example 2 nourishing cosmetic Water (emulsion)

According to a general method, a nourishing lotion was prepared with the composition described in table 8.

[ dosage form example 3 ] nourishing cream

A nourishing cream was prepared according to the usual method with the composition described in table 9.

[ dosage form example 4 ] massage cream

Massage creams were prepared according to the usual method with the composition described in table 10.

Dosage form example 5 facial mask

Facial masks were prepared according to the usual method with the composition described in table 11.

While exemplary embodiments have been shown and described, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

In addition, many modifications may be made to adapt a particular situation or material to the teachings of the invention without departing from the essential scope thereof. Therefore, it is intended that the invention not be limited to the particular exemplary embodiment disclosed as the best mode contemplated for carrying out this invention, but that the invention will include all embodiments falling within the scope of the appended claims.

Claims (11)

1. A cosmetic composition comprises a mixture of green tea saponin as an active ingredient, epigallocatechin gallate (EGCG) extracted from green tea leaves, and a culture product of green tea stem cells,

wherein the green tea saponin and the epigallocatechin gallate (EGCG) are respectively in an amount of 0.001-1 wt% based on the total weight of the composition,

wherein the amount of the stem cell culture product is 0.01-10 wt% of the total weight of the composition.

2. The cosmetic composition according to claim 1, wherein the green tea saponin is a substance obtained by extracting and removing a sugar residue from green tea seed husk.

3. The cosmetic composition according to claim 1, wherein the stem cells are callus-derived stem cells.

4. The cosmetic composition according to claim 1, wherein the stem cell culture product is one or more selected from the group consisting of a stem cell strain, a lysate thereof, an extract thereof, and a culture solution thereof.

5. Use of a mixture of green tea saponin, epigallocatechin gallate (EGCG) extracted from green tea and culture product of stem cells of green tea for the preparation of a cosmetic composition for combating skin aging,

wherein the green tea saponin and the epigallocatechin gallate (EGCG) are respectively in an amount of 0.001-1 wt% based on the total weight of the composition,

wherein the amount of the stem cell culture product is 0.01-10 wt% of the total weight of the composition.

6. Use of a mixture of green tea saponin, epigallocatechin gallate (EGCG) extracted from green tea and culture product of stem cells of green tea for the preparation of a cosmetic composition for moisturizing skin or enhancing skin barrier function,

wherein the green tea saponin and the epigallocatechin gallate (EGCG) are respectively in an amount of 0.001-1 wt% based on the total weight of the composition,

wherein the amount of the stem cell culture product is 0.01-10 wt% of the total weight of the composition.

7. Use according to claim 6, characterized in that said composition is capable of activating the filaggrin gene.

8. Use of a mixture of green tea saponin, epigallocatechin gallate (EGCG) extracted from green tea and culture product of stem cells of green tea for the preparation of a cosmetic composition for whitening skin or inhibiting skin pigmentation,

wherein the green tea saponin and the epigallocatechin gallate (EGCG) are respectively in an amount of 0.001-1 wt% based on the total weight of the composition,

wherein the amount of the stem cell culture product is 0.01-10 wt% of the total weight of the composition.

9. Use according to claim 8, characterized in that said composition is capable of activating the interleukin 6 gene.

10. Use of a mixture of green tea saponin, epigallocatechin gallate (EGCG) extracted from green tea and culture product of stem cells of green tea for the preparation of a cosmetic composition for enhancing skin elasticity or improving skin wrinkles,

wherein the green tea saponin and the epigallocatechin gallate (EGCG) are respectively in an amount of 0.001-1 wt% based on the total weight of the composition,

wherein the amount of the stem cell culture product is 0.01-10 wt% of the total weight of the composition.

11. Use according to claim 10, characterized in that said composition is capable of activating a catalase gene.