KR20100083888A - A cosmetic composition comprising tissue cultured panax ginseng adventitious roots extract and an preparing method thereof - Google Patents

Abstract

PURPOSE: A cosmetic composition containing wild ginseng adventitious root extract is provided to enhance skin moisturization effect without skin irritation. CONSTITUTION: A cosmetic composition for skin moisturization and skin protection contains 0.1-10 weight% of wild ginseng adventitious root extract as an active ingredient. The extract is isolated using purified water, low alcohol of C1-C5, polyvalent alcohol or mixture thereof as a solvent. A method for manufacturing the composition comprises: a step of tissue cultured-wild ginseng adventitious root with purified water, low alcohol of C1-C5, polyvalent alcohol, or mixture thereof; a step of maturing at 10-120°C to obtain extract; and a step of manufacturing cosmetic composition containing the extract and cosmetic ingredients.

Description

A cosmetic composition comprising tissue cultured Panax ginseng adventitious roots extract and an preparing method etc.

The present invention relates to a cosmetic composition comprising a wild ginseng root extract of tissue culture having a skin protection and moisturizing effect without causing skin irritation and a method for producing the same.

The stratum corneum of the epidermis is also located on the outermost side of the skin and suppresses the loss of moisture and electrolytes outside the skin, thereby preventing the skin from drying and providing an environment for normal biochemical metabolism. In addition, it plays an important role in protecting the human body from external physical damage and chemicals or preventing the invasion of bacteria and viruses into the skin. Water from normal skin is 70% in the body and 20% in the stratum corneum to support the elasticity and flexibility of the skin. In addition, Natural Moisturizing Factor (NMF) and sebum are present in the stratum corneum and help maintain the water balance of the stratum corneum. If the amount of NMF and sebum is well balanced, the skin stays moist and soft.

However, despite this function of the skin, the skin is unable to retain moisture due to the harsh external environment, the polluted atmosphere, and drying due to aging, and thus the skin tends to be dried, thus increasing the need for a skin moisturizer.

A number of substances for moisturizing have been known, and as a skin moisturizer known until now, it is common to use a substance capable of increasing water retention in the stratum corneum, such as lipid components such as ceramide and essential fatty acids and lipid complexes. In addition, in order to make a formulation containing these substances, 20 to 50 kinds of various raw materials are generally applied to the product such as sodium lauryl sulfate, dimethyl sulfoxide, croton oil, sodium hydroxide, and the like. As a result, irritation such as inflammation, itching, and allergies could be caused to the skin.

When the skin is exposed to irritating substances, the stratum corneum is damaged, thereby increasing transepidermal water loss, which is an indicator of damage to the stratum corneum, which may be the primary cause of inflammatory reactions. Inflammatory reactions that can be caused by the use of cosmetics include irritation such as itching, stinging, burning, and erythema and edema. Contact dermatitis, sensory irritation, phototoxic dermatitis, etc. can be roughly distinguished, but it is difficult to clearly distinguish between these symptoms and often occurs in combination with each other. Therefore, researches have been conducted to obtain useful ingredients from natural herbal medicines that have a protective effect on skin moisturizing and skin irritation and have no skin irritation and ensure safety for the human body.

Generally, botanical classification of wild ginseng belongs to Ogapiaceae, flowering plants and pizza plants. Since wild ginseng is very demanding and cautious, it spends many years buried in the ground where environmental conditions are not appropriate. Natural wild ginseng is classified into 18 species, including cheonjong and jijong, depending on the condition of native soil. Cheonjong (natural wild ginseng), whose seeds have fallen off the mountain, naturally, wild animals or birds eat wild ginseng seeds, excreted in the mountains, and grew up, races where people have sown seeds, mountain goats that have already grown ginseng, artificially There are many kinds of wild ginseng, such as camphor ginseng (Ginseng), and old ginseng that looks like a child because of its age, but among them, natural ginseng has a completely different appearance and medicinal effect from its appearance and medicinal effect. I have it. In addition, it contains a large amount of physiologically active substances such as saponin, thus increasing immune function, inhibiting cancer cell growth, antidiabetic effect, strengthening heart and blood pressure, strengthening liver function, enhancing gastrointestinal function, tonic effect, enhancing brain function, aging inhibition, Efficacy in improving weakness and constitution, and has excellent pharmacological effects and has long been known as a mysterious medicine of the East. In Dongbogam, this was recorded as 'Bird', and it is a rare plant that has been treated as a mysterious medicine since ancient times, and has been used as a treatment agent for various diseases and aphrodisiac. Recently, in order to make up for the shortcomings of the rare and high value of wild ginseng, the root tissue of wild ginseng, which is mainly used as a medicine, is multiplied with the roots of the ginseng using general plant tissue culture technology, so that the wild ginseng roots of uniform and excellent quality can be grown within a short period of time. It is cultivated and produced at low cost and used for various foods and raw materials.

In connection with this, techniques related to the cosmetic composition containing the wild ginseng root and its manufacturing method have been developed. However, the above technique has the disadvantage of requiring the ripening period of wild ginseng root after the completion of manufacture in order to leach out useful components of wild ginseng root by itself for a certain period of time by using the wild ginseng root of the ginseng rather than the extract. In addition, the technology has a disadvantage in that it can not be used with other raw materials that have good efficacy but long-lasting stability in the production of cosmetics or products that want fast use for a short period of time due to the disadvantage of requiring a maturation period.

Thus, the present inventors can maintain a constant quality by maintaining a constant culture conditions, and also by using a plant tissue culture technology that has the advantage that can be cultured in a short period of time compared to the harvest of wild species, wild ginseng root muscle extract is cultured Unlike the case of using the root muscle itself, it does not require a aging process after completion of preparation, and has a skin protection effect against external stimuli, and the cosmetic composition including the extract does not induce skin irritation and has a skin moisturizing and skin protection effect. The present invention was completed by confirming that the product was obtained.

Accordingly, an object of the present invention is to provide a cosmetic composition comprising a tissue cultured wild ginseng root extract having an effective effect on skin moisturizing and skin irritation while overcoming the disadvantages of the cosmetics including wild ginseng root muscle itself. .

Another object of the present invention is to provide a method for producing the cosmetic composition.

In order to achieve the above object, the present invention provides a cosmetic composition comprising a wild ginseng root extract of tissue culture as an active ingredient having a skin moisturizing and skin protection effect.

The cosmetic composition of the present invention may contain 0.1 to 10% by weight of the ginseng root muscle extract cultured tissue.

Tissue cultured wild ginseng root of ginseng extract, the tissue cultured wild ginseng root may be filtrate extracted using one or more solvents selected from the group consisting of purified water, C1 to C5 lower alcohols, polyhydric alcohols and mixtures thereof.

The solvent may be a mixed solvent containing 60 to 95% by weight of C1 to C5 lower alcohol and 5 to 40% by weight of polyhydric alcohol based on the total weight of the solvent.

The polyhydric alcohol is at least one selected from the group consisting of propylene glycol, butylene glycol and pentylene glycol.

The ginseng root muscle extract cultured in the tissue, may be prepared by adding 1 to 20% by weight of wild ginseng root muscle to 80 to 99% by weight of the solvent.

In addition, the present invention

i) mixing tissue cultured wild ginseng root muscle with purified water, C1 to C5 lower alcohol, polyhydric alcohol, or a mixed solvent thereof;

Iii) aging the mixture at 10 ° C. to 120 ° C. followed by filtration to obtain the filtrate, and

Iii) providing a cosmetic composition for skin moisturizing and skin protection, comprising the step of preparing a cosmetic composition comprising the filtrate and the cosmetic component.

By using the cosmetic composition to which the tissue-cultivated wild ginseng root extract of the present invention is added, it provides a moisturizing effect that protects the skin from skin drying without causing skin irritation and a cell protection effect against stimulation, thereby protecting skin and improving skin. Can contribute to

Hereinafter, the present invention will be described in detail.

The composition containing the wild ginseng root muscle extract of the tissue culture of the present invention,

i) mixing the cultured wild ginseng root muscle with an extraction solvent;

Ii) aging the mixture and filtering to obtain the filtrate, and

Iii) preparing a cosmetic composition comprising the filtrate and the cosmetic component

It is prepared by the method for producing a skin moisturizing and skin protection cosmetic composition comprising a.

Tissue cultured wild ginseng root of the present invention is a plant tissue culture method known to those skilled in the art, the wild ginseng root is characterized in that obtained by the method described in Korean Patent No. 0353636.

Tissue cultured extract of wild ginseng root of the present invention can be obtained by the following manufacturing process. That is, the ginseng root muscle of the tissue culture is at least one extraction solvent selected from the group consisting of purified water, C1 to C5 lower alcohols, polyhydric alcohols, and mixtures thereof, about 10 ° C to 120 ° C, preferably 10 ° C to 40 ° C. It can be obtained as a filtrate after aging at about 1 hour to 50 days, preferably 24 hours to 35 days. In addition, the extract of the present invention may be obtained further comprising the step of removing the solvent from the extract.

Preferably, a lower solvent of C1 to C5 or a mixed solvent of C1 to C5 lower alcohol and a polyhydric alcohol may be used as the extraction solvent.

As the lower alcohol of C1 to C5, ethanol may be preferably used. When preparing wild ginseng root extract using only ethanol alone, 1 to 20% by weight of dried cultured wild ginseng root root in tissues is ethanol 80 to 99 weight. It is preferable to add to%. At this time, the concentration of the ethanol is preferably about 60 to 90%, preferably about 65 to 80%.

In addition, when using a mixed solvent of C1 to C5 lower alcohol and polyhydric alcohol, it is preferable to add 1 to 20% by weight of the dried wild ginseng root muscle tissue cultured to 80 to 99% by weight of the mixed solvent. At this time, the mixed solvent preferably contains 60 to 95% by weight of C1 to C5 lower alcohol, 5 to 40% by weight of polyhydric alcohol with respect to the total weight of the solvent, and the concentration of the lower alcohol, for example, ethanol is about 60 90%, preferably about 65-80%, more preferably about 70%. When the content of the lower alcohol and the polyhydric alcohol of C1 to C5 in the mixed solvent is within the above range, it is easy to obtain an extract containing the desired components from wild ginseng root muscle due to the appropriate polarity. In addition, propylene glycol, butylene glycol or pentylene glycol may be used as the polyhydric alcohol, but is not limited thereto. Preferably, as the mixed solvent, a mixed solvent of 70% ethanol and propylene glycol, or a mixed solvent of 70% ethanol and butylene glycol may be used.

The aging method may be a method of using a reflux device in a water bath of immersion or water at 25 to 100 ° C., but is not limited thereto. You can get it.

In addition, in the method of obtaining the filtrate, after filtration, it is possible to further perform the process of decolorization and deodorization.

The present invention provides a cosmetic composition containing the extract obtained by the above manufacturing process as an active ingredient. In the cosmetic composition of the present invention, one or more wild ginseng extracts extracted by the above method may be mixed and used.

In the composition of the present invention, the extract is preferably contained in 0.1 to 10% by weight based on the total weight of the composition. If the extract is less than 0.1% by weight, the moisturizing and skin protection effects are weak due to lack of useful ingredients, and when the extract exceeds 10% by weight, there is a possibility of causing instability of product properties and irritation to the skin of the user.

In addition, the present inventors proved that the tissue-cultivated wild ginseng root muscle extract obtained by the manufacturing process had a skin protective effect by confirming the cellular protective effect against external stimulants and the enzyme inhibition causing inflammation, and also containing the extract. The excellent moisturizing effect of one cosmetic composition was confirmed.

The composition of the present invention can be used in a variety of cosmetics, face wash and shampoo for skin moisturizing and skin protection. The composition of the present invention is not limited to the formulation, and may take a formulation known in the art. For example, the formulation of the cosmetic composition according to the present invention, for example, various skin lotions, skin softeners, skin toners, astringents, lotions, milk lotions, nutrition lotions, massage creams, nutrition creams, hand creams, foundations, essences , Nutrition essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser, and the like, but is not limited thereto. In addition, the cosmetic composition of the present invention, in addition to the extract, the usual cosmetic components, such as solvents, extender pigments, inorganic pigments, organic pigments, inorganic powders, emulsions, surfactants that can be blended in cosmetics, silicone-containing surfactants, Thickeners, stabilizers, preservatives, antioxidants and fragrances can be blended within the range not impairing the object and effect of the present invention.

Hereinafter, the present invention will be described in detail with reference to the following examples and experimental examples. However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Reference Example 1. Preparation of wild ginseng root muscle in tissue culture

The wild ginseng root used in the present invention was used at Chungbuk National University Advanced Horticultural Technology Development Research Center. The wild ginseng root muscle is cultured using Murashige & Skoog medium (MS medium, Duchefa, Netherland) containing indolebutyl acid (IBA), a growth regulator, and sugar (sucrose) and vitamins, which are carbon sources. It was cultured and mass-proliferated by the method described in (Refer to Korean Patent No. 353636) and dried before use.

Reference Example 2 Human Fibroblast Cell Culture

10 cm cell containing 10 ml of Iscove's modified Dulbecco's medium (IMDM, GIBCO, USA) supplemented with 10% fetal calf serum (FBS, Invitrogen, USA) and 1% penicillin-streptomycin (Invitrogen, USA) The culture dish was inoculated with CCD-1064sk human fibroblasts (ATCC number: CRL-2076) purchased from ATCC. The cells were incubated in a 37 ° C., 5% CO ₂ atmosphere incubator until the cells reached 70-80% confluency. The medium was changed twice a week, and the cells that reached the confluence were maintained after trypsinization using trypsin-EDTA (Invitrogen, USA).

Examples 1-4. Tissue cultured wild ginseng root Preparation of Extract

As shown in Table 1 below, 3 g of dried wild ginseng root root prepared in Reference Example 1 was mixed with 97 g of a mixed solvent of 70% ethanol and 1,3-butylene glycol or propylene glycol. The mixture was immersed for 5 weeks and aged, and then filtered through a filter paper (5C, 185 mm, Toyo roshi Kaisha, Ltd., Japan) to obtain a wild ginseng root extract (Examples 1 to 4) in the filtrate state.

Formulation Examples 1-3. Preparation of supple cosmetics (skin) comprising wild ginseng root extract of Example 1

To the composition ratio shown in Table 2 below, the softening water (skin) containing wild ginseng root extract of Example 1 was prepared.

Formulation Examples 4-6. Preparation of nutritional milk (milk lotion) comprising the extract of Example 1

To the composition ratio shown in Table 3, was prepared nutritional milk (milk lotion) containing wild ginseng extract of Example 1.

Formulation Examples 7-9. Preparation of a cream comprising the extract of Example 1

To the composition ratio shown in Table 4 below, a cream containing wild ginseng extract of Example 1 was prepared.

Formulation Examples 10-12. Preparation of a powder comprising the extract of Example 1

To the composition ratio shown in Table 5, to prepare a powder containing wild ginseng root extract of Example 1.

Formulation Examples 13-15: Preparation of a Foundation Containing the Extract of Example 1

To the composition ratio shown in Table 6 below, a foundation containing a wild ginseng root extract of Example 1 was prepared.

Formulation Examples 16-18: Preparation of a makeup base comprising the extract of Example 1

To the composition ratio shown in Table 7 below, a makeup base containing wild ginseng extract of Example 1 was prepared.

As described below, the cytoprotective effect on the external stimulation of the extract of wild ginseng root extract prepared in the above Example was measured, and the moisturizing effect and skin irritation relief effect of the cosmetic formulation example containing the extract was measured.

Comparative Examples 1 and 2: Preparation of Cosmetics (Flexible Longevity) That Does Not Contain Both Wild Ginseng Verbena Extract and Wild Ginseng Abdominal Muscle itself

In the composition of Formulation Example 1, the cosmetics (Comparative Example 1) that does not include both wild ginseng root extract and wild ginseng root muscle (Comparative Example 2), including wild ginseng root muscle itself, are shown in Table 8 below. Except for both the root extract and wild ginseng root muscle or was prepared according to the composition containing the wild ginseng root muscle instead of the wild ginseng root muscle extract. At this time, the content of wild ginseng root muscle of Table 8 is the content ratio (weight ratio) of wild ginseng root muscle extract obtained from wild ginseng root muscle when using a mixed solvent of 70% ethanol and 1,3-butylene glycol or propylene glycol as a solvent. Was determined to be approximately 1: 5.

Test Example 1. Measurement of Cell Protective Effect of Cosmetics Containing Wild Ginseng Extract

The protective effect of Formulation Example 1 cosmetics against external stimuli was compared with the protective effect of Cosmetics of Comparative Examples 1 and 2.

1-1. Determination of the protective effect against SDS (Sodium dodecyl sulfate) stimulation

Human fibroblasts cultured according to Reference Example 2 were dispensed at 1 × 10 ⁴ cells / well in 96-multiwell plates (SPL, Korea) respectively and incubated for 24 hours. Thereafter, the medium was replaced with IMDM added with 2% FBS, and each measurement formulation was diluted 10-fold in culture medium and centrifuged at 5,000 g for 20 minutes to obtain a supernatant therefrom. 1 hour after diluting in the medium, and then treated with SDS 0.1 mM in parallel and incubated for 24 hours. In cultured cells, MTT stock solution (5 mg / ml) dissolved in PBS (phosphate buffered saline) was diluted 10-fold in fresh medium, and 100 µl of each solution was added to each well of the cell, and then 4 hours at 37 ° C. Reacted. After the reaction, the living cells were dehydrated by the dehydrogenase present in the mitochondria, and the yellow water-soluble substrate MTT [3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium-bromide] was insoluble in MTT formazan. is reduced to formazan crystals, and the amount of this can be used to determine cell viability. The culture supernatant was removed, and 200 μl of DMSO was added to each well to dissolve MTT formazan crystals produced in the cells, and the absorbance was measured as follows by an absorption spectrophotometer (UVmax, Molecular Device, USA). The difference between the OD values at the wavelength of 540 nm, the test filter for which the absorbance of formazan is maximized, and the OD value at the wavelength of 630 nm, the reference filter, is obtained, and the difference value is calculated by Equation 1 below. The cell protection rate according to concentration was calculated and shown in Table 9 (see The EMBO Journal (2002) 21, pp 2407-2417, etc.). The measurement test was carried out three times in three batches, and the standard deviation was calculated by calculating the average absorbance based on the raw data.

As shown in Table 9, the formulation containing the wild ginseng root extract of the present invention (Formulation of Formulation Example 1) increased the protective effect against SDS stimulation compared to the formulation of Comparative Examples 1 and 2. From this, it was confirmed that the formulation containing the wild ginseng root muscle extract of the present invention has an increased protective effect against external stimulation than the formulation including the wild ginseng root muscle itself.

Test Example 2 Measurement of Inhibition of Lipoxygenase Activity

The activity inhibition test of lipoxygenase, a representative enzyme of inflammatory factors against external stimuli, was performed. The lipoxygenase activity pathway and cycloxygenase activity pathway are representative of the pathways of enzymes that generate inflammation by stimulation. Of these, lipoxygenase catalyzes the hydroperoxidation of unsaturated fatty acids to produce fatty hydroperoxides, which in turn produce degradation products such as epoxides, hydroxyls, ketones, aldehydes and the like. Lipoxygenase produces a variety of chemical mediators involved in inflammatory reactions or allergies in vivo from arachidonic acid biosynthesized from linolenic acid. Therefore, a substance that inhibits lipoxygenase has a result of inhibiting the production of chemical mediators involved in various inflammatory reactions or allergies. 0.1 ml of the sample to be measured and 1 ml of lipoxygenase (Sigma-aldrich, USA) were mixed in 1 ml of 1 mM linolenic acid (Sigma-aldrich, USA) and reacted for 10 minutes at 25 ° C. after voltexing. Then, 0.5 ml of 20% trichloroacetic acid and 1 ml of 0.6% thiobarbituric acid were added and reacted with boiling water for 10 minutes, and then cooled in ice water for 2-3 minutes, butanol After 2 ml of vortexing for 20 seconds or more, centrifuged at 3500 rpm for 5 minutes, and measuring the OD value at 513 nm of UV spectrophotometer to determine the inhibition rate of lipoxygenase activity according to the following equation (2). The calculation is shown in Table 10 below. The measurement test was carried out three times in three batches, and the standard deviation was calculated by calculating the average absorbance based on the raw data.

As shown in Table 10, the formulation containing the tissue cultured wild ginseng root extract of Formulation Example 1 inhibited lipoxygenase in a concentration-dependent manner compared to the untreated control, and also included the wild ginseng root muscle itself (compared to It was confirmed that the formulation of Example 2 has an effect of inhibiting the activity of the enzyme causing inflammation by external stimulation.

Test Example 2 Measurement of Skin Moisturizing Effect and Safety of Formulation Example of Tissue Cultured Wild Ginseng Extract

The cream containing the tissue cultured wild ginseng root muscle extract prepared above was tested for safety through a skin moisturizing test and detection of skin irritation.

2-1. Skin moisturizing effect measurement

In order to investigate the moisturizing effect of the cosmetic according to the present invention, the moisture content of the skin was measured. After preparing the cream formulation containing the wild ginseng root muscle extract of Example 1 (or Example 3) and the formulation containing no extract as shown in Table 11, 20 women aged 20 to 50 years The moisture content of the skin before and after application was measured as follows.

In order to measure the skin moisturizing effect, skin moisture content and skin moisture loss (TEWL: Trans Epidermal Water Loss) are respectively measured by a skin moisture content meter (Corneometer CM 825, Courage + Khazaka electronic company, Germany) and a skin moisture loss meter (Tewameter TM 210). , Courage + Khazaka electronic company, Germany). Each sample is uniformly applied at a concentration of 2.0 mg / cm 2 to the upper and lower portions of the applicant waiting in the room at constant temperature and humidity conditions (20-22 ° C, relative humidity 40-60%) one hour before the start of the test, and the sample is applied. The other upper arm, which was not, was used as a control. The skin moisture content and skin moisture loss amount immediately before application were measured, and the skin moisture content and skin moisture loss amount after 1 hour, 3 hours, and 5 hours were measured and are shown in Tables 12 and 13, respectively.

As shown in Table 12 and Table 13, wild ginseng root muscle itself (Comparative Example 3) or wild ginseng root muscle extract (Formulation Example 7) compared to the site to which the formulation of the present invention to which the extract of the present invention was not added (Formulation of Comparative Example 4) was applied. The area to which the cream containing was applied had a high skin moisture content and a small amount of skin moisture loss. In addition, the cream formulation of Formulation Example 7 containing wild ginseng root muscle extract had a higher skin moisture content than the formulation of Comparative Example 3 in which wild ginseng root muscle was added, and the skin moisture loss amount was less effective. According to the above, it was confirmed that the formulation containing the wild ginseng root extract of the present invention has an excellent moisture content maintaining effect and inhibiting skin water loss compared to the formulation containing no additive or wild ginseng root.

2-2. Safety measurement for skin irritation by human skin irritation test

Human patch tests to assess skin primary irritation were conducted with minor modifications to the methods presented in the American Cosmetics Association (CTFA) guidelines. After preparing each cream formulation (Formulation Example 7, Comparative Example 3, Comparative Example 4) shown in Table 11 of Test Example 2-1, the safety by the application of each formulation was measured as follows.

Twenty women aged 20 to 50 years were wiped and dried with 70% ethanol, and then the Hayes' chamber (Haye's chamber, Haye's Service, Netherland) to which 20 mg of each formulation was applied was attached to the test site. . After 24 hours, the haze chamber was removed and the first reading was made. After 30 minutes, 24 hours, and 48 hours, the presence of irritation was evaluated. Evaluation criteria were in accordance with the evaluation criteria (see Table 14) of the International Contact dermatitis Research Group (ICDRG), the evaluation of skin irritation probability was performed by the average value calculated according to the following equation (3).

The degree of primary stimulation is average 0 to 0.99, no irritation, 1.0 to 1.99 slight irritation, 2.0 to 2.99 moderate irritation, 3.0 to 4.99 strong irritation), five or more were evaluated as severe irritation, and the results are shown in Table 15 below.

As shown in Table 15, as a result of the evaluation of the human skin patch test and subjective irritation of the formulation containing the wild ginseng root extract, the formulation containing the wild ginseng root extract (formulation of Formulation Example 7) is the additive-free formulation (Comparative Example 4 Compared to the formulation including the wild ginseng muscle itself (formulation of Comparative Example 3), the average value of the primary stimulus showed a lower value, which did not cause skin irritation, thereby preventing the skin irritation of the extract of the present invention. It could be confirmed that excellent.

Claims (7)

Skin moisturizing and skin protection cosmetic composition comprising a cultured wild ginseng root extract as an active ingredient. The cosmetic composition for skin moisturizing and skin protection according to claim 1, wherein the extract is contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic composition. The method of claim 1, wherein the extract is moisturized skin, characterized in that the wild ginseng extract extracted with at least one solvent selected from the group consisting of purified water, C1 to C5 lower alcohol, polyhydric alcohol and mixtures thereof Cosmetic composition for skin protection. The method of claim 3, wherein the solvent is a mixed solvent containing 60 to 95% by weight of C1 to C5 lower alcohol and 5 to 40% by weight of a polyhydric alcohol with respect to the total weight of the solvent, skin moisturizing and skin protection cosmetics Composition. The method of claim 4, wherein the polyhydric alcohol is propylene glycol, butylene glycol and pentylene glycol, characterized in that at least one selected from the group consisting of, moisturizing skin and cosmetic composition for skin protection. The cosmetic composition for skin moisturizing and skin protection according to claim 4, wherein the extract is prepared by adding 1 to 20% by weight of the wild ginseng root muscle to 80 to 99% by weight of the solvent. i) mixing the cultured wild ginseng root muscle with purified water, C1 to C5 lower alcohol, polyhydric alcohol, or a mixed solvent thereof; Ii) aging the mixture at 10 ° C. to 120 ° C. and filtering to obtain a filtrate, and Iii) preparing a cosmetic composition comprising the filtrate and the cosmetic component The manufacturing method of the cosmetic composition for skin moisturizing and skin protection as described in any one of Claims 1-6 containing them.