HK1166820A1 - Improved detection of bacterial (mollicutes) contamination () - Google Patents

Improved detection of bacterial (mollicutes) contamination ()

Info

Publication number
HK1166820A1
HK1166820A1 HK12107368.5A HK12107368A HK1166820A1 HK 1166820 A1 HK1166820 A1 HK 1166820A1 HK 12107368 A HK12107368 A HK 12107368A HK 1166820 A1 HK1166820 A1 HK 1166820A1
Authority
HK
Hong Kong
Prior art keywords
mollicutes
bacterial
contamination
improved detection
detection
Prior art date
Application number
HK12107368.5A
Other languages
English (en)
Chinese (zh)
Inventor
Christian Birkner
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of HK1166820A1 publication Critical patent/HK1166820A1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
    • C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
    • C12Q2549/125—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals using sterilising/blocking agents, e.g. albumin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK12107368.5A 2009-08-01 2012-07-26 Improved detection of bacterial (mollicutes) contamination () HK1166820A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP09009965 2009-08-01
PCT/EP2010/004655 WO2011015312A1 (en) 2009-08-01 2010-07-29 Improved detection of bacterial (mollicutes) contamination

Publications (1)

Publication Number Publication Date
HK1166820A1 true HK1166820A1 (en) 2012-11-09

Family

ID=43085928

Family Applications (1)

Application Number Title Priority Date Filing Date
HK12107368.5A HK1166820A1 (en) 2009-08-01 2012-07-26 Improved detection of bacterial (mollicutes) contamination ()

Country Status (9)

Country Link
US (4) US20120129181A1 (xx)
EP (1) EP2459745B1 (xx)
JP (1) JP5520376B2 (xx)
CN (1) CN102471804B (xx)
CA (1) CA2769043C (xx)
ES (1) ES2527684T3 (xx)
HK (1) HK1166820A1 (xx)
SG (1) SG176706A1 (xx)
WO (1) WO2011015312A1 (xx)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2868391A1 (en) 2012-04-02 2013-10-10 Stephane Bancel Polynucleotides comprising n1-methyl-pseudouridine and methods for preparing the same
CN103627781A (zh) * 2012-08-24 2014-03-12 华北制药集团新药研究开发有限责任公司 一种检测cho培养细胞中支原体污染的试剂盒及其检测方法
CA2923029A1 (en) 2013-09-03 2015-03-12 Moderna Therapeutics, Inc. Chimeric polynucleotides
SG11202000127YA (en) * 2017-12-08 2020-06-29 Terumo Corp Method for testing presence or absence of mycoplasma

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007053491A2 (en) * 2005-10-28 2007-05-10 The Trustees Of Columbia University In The City Of New York Method and kit for evaluating rna quality
CN100999763A (zh) * 2006-01-12 2007-07-18 张曼 一种应用改良引物检测肿瘤中细胞分裂周期蛋白基因6的方法
US20090217399A1 (en) * 2006-03-17 2009-08-27 Massachusetts Institute Of Technology Lentiviral Vectors That Provide Improved Expression And Reduced Variegation After Transgenesis
EP2098599A1 (en) * 2008-03-05 2009-09-09 Deutsches Krebsforschungszentrum, Stiftung des öffentlichen Rechts Composition comprising an oligonucleotide mixture for the detection of contaminations in cell cultures
CN101492741A (zh) * 2009-01-22 2009-07-29 中国农业科学院北京畜牧兽医研究所 一种定量检测猪肺炎支原体的方法

Also Published As

Publication number Publication date
CA2769043C (en) 2017-09-12
US20150086990A1 (en) 2015-03-26
CN102471804A (zh) 2012-05-23
US20120129181A1 (en) 2012-05-24
US11078545B2 (en) 2021-08-03
CA2769043A1 (en) 2011-02-10
WO2011015312A1 (en) 2011-02-10
JP5520376B2 (ja) 2014-06-11
ES2527684T3 (es) 2015-01-28
US20210324453A1 (en) 2021-10-21
EP2459745A1 (en) 2012-06-06
CN102471804B (zh) 2015-03-11
US20170292148A1 (en) 2017-10-12
SG176706A1 (en) 2012-01-30
JP2013500702A (ja) 2013-01-10
EP2459745B1 (en) 2014-11-12

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