HK1048497A1 - 穩定腈水解酶活性及保存微生物細胞的方法 - Google Patents

穩定腈水解酶活性及保存微生物細胞的方法

Info

Publication number
HK1048497A1
HK1048497A1 HK03100618.9A HK03100618A HK1048497A1 HK 1048497 A1 HK1048497 A1 HK 1048497A1 HK 03100618 A HK03100618 A HK 03100618A HK 1048497 A1 HK1048497 A1 HK 1048497A1
Authority
HK
Hong Kong
Prior art keywords
stabilizing
microbial cells
nitrilase activity
preserving microbial
preserving
Prior art date
Application number
HK03100618.9A
Other languages
English (en)
Inventor
Dicosimo Robert
Ben-Bassat Arie
D Fallon Robert
Original Assignee
納幕爾杜邦公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 納幕爾杜邦公司 filed Critical 納幕爾杜邦公司
Publication of HK1048497A1 publication Critical patent/HK1048497A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
HK03100618.9A 1999-07-12 2003-01-23 穩定腈水解酶活性及保存微生物細胞的方法 HK1048497A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/352,015 US6251646B1 (en) 1999-07-12 1999-07-12 Method for stabilizing nitrilase activity and preserving microbial cells
PCT/US2000/018908 WO2001004278A1 (en) 1999-07-12 2000-07-12 Method for stabilizing nitrilase activity and preserving microbial cells

Publications (1)

Publication Number Publication Date
HK1048497A1 true HK1048497A1 (zh) 2003-04-04

Family

ID=23383443

Family Applications (2)

Application Number Title Priority Date Filing Date
HK02104298.9A HK1042322A1 (zh) 1999-07-12 2002-06-06 穩定腈水解酶活性及保存微生物細胞的方法
HK03100618.9A HK1048497A1 (zh) 1999-07-12 2003-01-23 穩定腈水解酶活性及保存微生物細胞的方法

Family Applications Before (1)

Application Number Title Priority Date Filing Date
HK02104298.9A HK1042322A1 (zh) 1999-07-12 2002-06-06 穩定腈水解酶活性及保存微生物細胞的方法

Country Status (9)

Country Link
US (1) US6251646B1 (zh)
EP (1) EP1194533A1 (zh)
JP (1) JP2003504049A (zh)
KR (1) KR20020059332A (zh)
CN (1) CN1160456C (zh)
CA (1) CA2377101A1 (zh)
HK (2) HK1042322A1 (zh)
MX (1) MXPA02000422A (zh)
WO (1) WO2001004278A1 (zh)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6551804B2 (en) * 1999-07-12 2003-04-22 E. I. Du Pont De Nemours And Company Process for preparing 4-cyanopentanoic acid
US6677149B2 (en) * 1999-07-12 2004-01-13 E. I. Du Pont De Nemours And Company Method for stabilizing nitrilase activity and preserving microbial cells with carbamate salts
EP1280892B1 (en) * 2000-03-31 2011-01-12 E.I. Du Pont De Nemours And Company ISOLATION AND EXPRESSION OF A GENE FOR A NITRILASE FROM i ACIDOVORAX FACILIS 72W
US8815569B2 (en) * 2003-01-08 2014-08-26 Basf Se Methods for preserving and/or storing cells having a nitrilase or nitrile hydratase activity
DE602004024329D1 (de) * 2003-12-02 2010-01-07 Ciba Spec Chem Water Treat Ltd Herstellung von amiden
US20100267100A1 (en) * 2007-03-21 2010-10-21 Ling Hua Enzymatic synthesis of optically pure beta-hydroxy carboxylic acids and their derivatives
US20090004720A1 (en) * 2007-06-26 2009-01-01 Ling Hua Smart Biocatalysts For Organic Synthesis
US7741088B2 (en) * 2007-10-31 2010-06-22 E.I. Dupont De Nemours And Company Immobilized microbial nitrilase for production of glycolic acid
US7695945B2 (en) * 2007-10-31 2010-04-13 E. I. Du Pont De Nemours And Company Immobilized microbial nitrilase for production of glycolic acid
CN104911174A (zh) * 2015-06-05 2015-09-16 浙江工业大学 一种含腈水解酶细胞的固定化方法
CN104911225A (zh) * 2015-06-05 2015-09-16 浙江工业大学 一种化学-酶法生产加巴喷丁的方法
CN107779481A (zh) * 2016-08-29 2018-03-09 中国石油化工股份有限公司 丙烯酰胺水溶液的制备方法
CN111172140B (zh) * 2020-01-21 2022-04-19 浙江工业大学 一种腈水解酶突变体及其在制备抗癫痫药物中间体中的应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3037009A1 (de) 1979-10-04 1981-04-23 Nitto Chemical Industry Co., Ltd., Tokyo Verfahren zur herstellung von acrylamid aus acrylnitril unter verwendung von mikroorganismen
JPS62257386A (ja) 1986-05-02 1987-11-09 Nitto Chem Ind Co Ltd ニトリル水和活性の保持方法
JPS62259586A (ja) 1986-05-02 1987-11-11 Nitto Chem Ind Co Ltd ニトリル水和活性の保持方法
US5629190A (en) 1992-08-10 1997-05-13 Rhone-Poulenc Chimie Polypeptides possessing a nitrilase activity and method of converting nitriles to carboxylates by means of said polypeptides
JP3163224B2 (ja) 1994-10-14 2001-05-08 三菱レイヨン株式会社 菌体または固定化菌体の懸濁液の保存方法
US5858736A (en) * 1996-05-17 1999-01-12 E. I. Du Pont De Nemours And Company Preparation of lactams from aliphatic α,ω-dinitriles
JPH1042885A (ja) 1996-08-02 1998-02-17 Mitsui Petrochem Ind Ltd 微生物によるアミド類及び、または有機酸類の製造法

Also Published As

Publication number Publication date
JP2003504049A (ja) 2003-02-04
CA2377101A1 (en) 2001-01-18
CN1361820A (zh) 2002-07-31
EP1194533A1 (en) 2002-04-10
WO2001004278A1 (en) 2001-01-18
US6251646B1 (en) 2001-06-26
HK1042322A1 (zh) 2002-08-09
KR20020059332A (ko) 2002-07-12
MXPA02000422A (es) 2002-07-30
CN1160456C (zh) 2004-08-04

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