HK1041285B - 運用一個加工酵素的多核甘酸合成法 - Google Patents

運用一個加工酵素的多核甘酸合成法

Info

Publication number
HK1041285B
HK1041285B HK02101110.1A HK02101110A HK1041285B HK 1041285 B HK1041285 B HK 1041285B HK 02101110 A HK02101110 A HK 02101110A HK 1041285 B HK1041285 B HK 1041285B
Authority
HK
Hong Kong
Prior art keywords
polynucleotide synthesis
enzyme
processing enzyme
polynucleotide
nucleotide
Prior art date
Application number
HK02101110.1A
Other languages
English (en)
Other versions
HK1041285A1 (en
Inventor
Daniel Henry Densham
Original Assignee
Medical Biosystems Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Biosystems Ltd filed Critical Medical Biosystems Ltd
Publication of HK1041285A1 publication Critical patent/HK1041285A1/xx
Publication of HK1041285B publication Critical patent/HK1041285B/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/215—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Halobacteriaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Low-Molecular Organic Synthesis Reactions Using Catalysts (AREA)
  • Saccharide Compounds (AREA)
HK02101110.1A 1999-04-06 2002-02-15 運用一個加工酵素的多核甘酸合成法 HK1041285B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9907813.1A GB9907813D0 (en) 1999-04-06 1999-04-06 Synthesis
PCT/GB2000/001289 WO2000060072A1 (en) 1999-04-06 2000-04-06 Polynucleotide synthesis using a processing enzyme

Publications (2)

Publication Number Publication Date
HK1041285A1 HK1041285A1 (en) 2002-07-05
HK1041285B true HK1041285B (zh) 2009-04-09

Family

ID=10851008

Family Applications (1)

Application Number Title Priority Date Filing Date
HK02101110.1A HK1041285B (zh) 1999-04-06 2002-02-15 運用一個加工酵素的多核甘酸合成法

Country Status (17)

Country Link
US (1) US6623929B1 (zh)
EP (1) EP1165786B1 (zh)
JP (1) JP2002540788A (zh)
KR (1) KR100671070B1 (zh)
CN (1) CN1161466C (zh)
AT (1) ATE400655T1 (zh)
AU (1) AU774308B2 (zh)
BR (1) BR0009530A (zh)
CA (1) CA2367269A1 (zh)
DE (1) DE60039421D1 (zh)
GB (1) GB9907813D0 (zh)
HK (1) HK1041285B (zh)
IL (1) IL145578A0 (zh)
IS (1) IS6088A (zh)
MX (1) MXPA01009998A (zh)
NZ (1) NZ514346A (zh)
WO (1) WO2000060072A1 (zh)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1009802B1 (en) 1997-02-12 2004-08-11 Eugene Y. Chan Methods for analyzimg polymers
RU2198221C2 (ru) * 1997-07-28 2003-02-10 Медикал Биосистемз Лтд. Способ секвенирования полинуклеотида и устройство для его осуществления
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6927065B2 (en) 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US6902921B2 (en) 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US6956114B2 (en) 2001-10-30 2005-10-18 '454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
WO2004091491A2 (en) * 2003-04-10 2004-10-28 Transform Pharmaceuticals, Inc. Profiling conformational variants, antibody compositions and methods of using the same
JP2005161480A (ja) * 2003-12-03 2005-06-23 Canon Inc 物体処理装置、物体の移動方法及びその移動方法を用いて処理された物体
US7595160B2 (en) 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
EP1704256A4 (en) 2004-01-13 2008-01-16 Us Genomics Inc DETECTION AND QUANTIFICATION OF ANALYTES IN SOLUTION USING POLYMERS
EP1774034A2 (en) * 2004-06-07 2007-04-18 The California Institute of Technology Detection of dna mismatches and oxidative lesions
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
CN104862383B (zh) 2008-03-28 2019-05-28 加利福尼亚太平洋生物科学股份有限公司 用于核酸测序的组合物和方法
FR3020071B1 (fr) 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
JP6951334B2 (ja) * 2015-12-22 2021-10-20 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 熱に不安定な色素を含む組成物
EP3665274A1 (en) 2017-08-07 2020-06-17 DNA Script Variants of family a dna polymerase and uses thereof
US10752887B2 (en) 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
WO2019135007A1 (en) 2018-01-08 2019-07-11 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
WO2020120442A2 (en) 2018-12-13 2020-06-18 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
AU2020223456A1 (en) 2019-02-12 2021-08-12 Dna Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE462454B (sv) * 1988-11-10 1990-06-25 Pharmacia Ab Maetyta foer anvaendning i biosensorer
DE4406524A1 (de) 1994-02-28 1995-08-31 Boehringer Mannheim Gmbh 3'-RNA-Markierung mit Terminaler Transferase
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
DK0655506T3 (da) 1994-10-17 1997-03-10 Harvard College DNA-polymerase med modificeret nukleotidbindingssted for DNA-sekvensanalyse
JP2000502882A (ja) 1995-09-08 2000-03-14 ライフ・テクノロジーズ・インコーポレイテッド サーモトガ由来のクローン化ｄｎａポリメラーゼ類およびそれらの変異体
DE19601385A1 (de) 1996-01-16 1997-07-17 Mueller Manfred W Verfahren zur Amplifikation von Nukleinsäure
EP0983364B1 (en) * 1997-03-12 2002-06-12 PE Corporation (NY) Dna polymerases having improved labeled nucleotide incorporation properties
RU2198221C2 (ru) * 1997-07-28 2003-02-10 Медикал Биосистемз Лтд. Способ секвенирования полинуклеотида и устройство для его осуществления
AU9123598A (en) 1997-08-29 1999-03-16 Life Technologies, Inc. High fidelity polymerases and uses thereof

Also Published As

Publication number Publication date
HK1041285A1 (en) 2002-07-05
DE60039421D1 (de) 2008-08-21
AU3978000A (en) 2000-10-23
CA2367269A1 (en) 2000-10-12
EP1165786B1 (en) 2008-07-09
CN1161466C (zh) 2004-08-11
AU774308B2 (en) 2004-06-24
GB9907813D0 (en) 1999-06-02
MXPA01009998A (es) 2002-08-20
IS6088A (is) 2001-09-26
NZ514346A (en) 2003-03-28
KR100671070B1 (ko) 2007-01-17
CN1348494A (zh) 2002-05-08
EP1165786A1 (en) 2002-01-02
IL145578A0 (en) 2002-06-30
WO2000060072A1 (en) 2000-10-12
JP2002540788A (ja) 2002-12-03
ATE400655T1 (de) 2008-07-15
BR0009530A (pt) 2002-02-05
KR20010111579A (ko) 2001-12-19
US6623929B1 (en) 2003-09-23

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Legal Events

Date Code Title Description
PC Patent ceased (i.e. patent has lapsed due to the failure to pay the renewal fee)

Effective date: 20130406