ATE84313T1 - Substratunterstuetzte katalyse. - Google Patents

Substratunterstuetzte katalyse.

Info

Publication number
ATE84313T1
ATE84313T1 AT88903692T AT88903692T ATE84313T1 AT E84313 T1 ATE84313 T1 AT E84313T1 AT 88903692 T AT88903692 T AT 88903692T AT 88903692 T AT88903692 T AT 88903692T AT E84313 T1 ATE84313 T1 AT E84313T1
Authority
AT
Austria
Prior art keywords
enzyme
catalytic
substrate
catalysis
mutant
Prior art date
Application number
AT88903692T
Other languages
English (en)
Inventor
James Allen Wells
Paul John Carter
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ATE84313T1 publication Critical patent/ATE84313T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
AT88903692T 1987-04-02 1988-03-30 Substratunterstuetzte katalyse. ATE84313T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3408587A 1987-04-02 1987-04-02
EP88903692A EP0308499B1 (de) 1987-04-02 1988-03-30 Substratunterstützte katalyse
PCT/US1988/001078 WO1988007578A1 (en) 1987-04-02 1988-03-30 Substrate assisted catalysis

Publications (1)

Publication Number Publication Date
ATE84313T1 true ATE84313T1 (de) 1993-01-15

Family

ID=21874212

Family Applications (1)

Application Number Title Priority Date Filing Date
AT88903692T ATE84313T1 (de) 1987-04-02 1988-03-30 Substratunterstuetzte katalyse.

Country Status (4)

Country Link
EP (1) EP0308499B1 (de)
AT (1) ATE84313T1 (de)
DE (1) DE3877300T2 (de)
WO (1) WO1988007578A1 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990004788A1 (en) * 1988-10-28 1990-05-03 Genentech, Inc. Method for identifying active domains and amino acid residues in polypeptides and hormone variants
US5688666A (en) * 1988-10-28 1997-11-18 Genentech, Inc. Growth hormone variants with altered binding properties
US5534617A (en) * 1988-10-28 1996-07-09 Genentech, Inc. Human growth hormone variants having greater affinity for human growth hormone receptor at site 1
US6780613B1 (en) 1988-10-28 2004-08-24 Genentech, Inc. Growth hormone variants
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
AU654563B2 (en) * 1991-07-24 1994-11-10 Imperial Chemical Industries Plc Proteins
DE69635026T2 (de) * 1995-09-21 2006-05-24 Genentech Inc., San Francisco Varianten des menschlichen wachstumshormons
CA2238296C (en) 1995-12-12 2008-07-22 Takara Shuzo Co., Ltd. Ultrathermostable protease genes
CN1136311C (zh) 1996-11-04 2004-01-28 诺沃奇梅兹有限公司 枯草杆菌酶变异体和组合物
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
JP2003505067A (ja) 1999-07-22 2003-02-12 ザ、プロクター、エンド、ギャンブル、カンパニー 特定のエピトープ領域にアミノ酸欠失及び置換を有するサブチリシンプロテアーゼの変異体
BR0012660A (pt) 1999-07-22 2002-04-09 Procter & Gamble Variante de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal
KR20020021395A (ko) 1999-07-22 2002-03-20 데이비드 엠 모이어 입체적으로 보호된 ｃｌｉｐ 부위를 갖는 프로테아제콘쥬게이트
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
CN108060170B (zh) * 2017-12-29 2020-05-15 江南大学 一种新型金属离子耐受性角蛋白酶及其应用

Also Published As

Publication number Publication date
EP0308499A1 (de) 1989-03-29
EP0308499B1 (de) 1993-01-07
DE3877300D1 (de) 1993-02-18
WO1988007578A1 (en) 1988-10-06
DE3877300T2 (de) 1993-06-03

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Legal Events

Date Code Title Description
EEIH Change in the person of patent owner
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee