GB862685A - New antibiotic designated pa 155a - Google Patents
New antibiotic designated pa 155aInfo
- Publication number
- GB862685A GB862685A GB33074/57A GB3307457A GB862685A GB 862685 A GB862685 A GB 862685A GB 33074/57 A GB33074/57 A GB 33074/57A GB 3307457 A GB3307457 A GB 3307457A GB 862685 A GB862685 A GB 862685A
- Authority
- GB
- United Kingdom
- Prior art keywords
- antibiotic
- ethyl acetate
- methanol
- broth
- solvent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/28—Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Pest Control & Pesticides (AREA)
- Biomedical Technology (AREA)
- Dentistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
<PICT:0862685/IV (b)/1> An antibiotic designated PA 155A is produced by cultivating a PA155A - producing strain of Streptomyces Albus e.g. S. Albus ATCC 126 48 in an aqueous nutrient medium containing a source of carbon, organic nitrogen and inorganic salts under aerobic and especially submerged aerobic conditions. The temperature of cultivation is 23 to 32 DEG C. and preferably 26 to 30 DEG C., the pH is 6 to 7 and the duration of cultivation 1 to 3 days. Specified carbon sources are sugar, starch and glycerol; organic nitrogen sources are soybean meal, cottonseed meal, peanut meal and corn steep liquor; mineral salts are sodium chloride, sodium nitrate, magnesium sulphate and potassium phosphate. A buffer such as calcium carbonate or potassium dihydrogen phosphate may also be present. The antibiotic PA155A is isolated from the clarified broth by (a) solvent extraction (b) absorption on cation exchange resins (c) column chromatography on acid washed alumina. In one method, the filtered broth is extracted with ethyl acetate, the extract concentrated and a mixture of PA 155A and another antibiotic PA 155B precipitated by the addition of a non-solvent such as petroleum ether. A third antibiotic PA 155X remains in the residual clarified broth. Further purification of the mixed PA 155A and B is obtained by column chromatography on acid washed alumina. Alternatively, the residue obtained by removing the solvent from the ethyl acetate extract is subjected to countercurrent distribution between benzene, methanol and water (2:1:1), the PA 115A being recovered from the aqueous layer and the PA 115B from the organic layer. PA 155X is recovered from the filtered broth by treatment with a strongly acid cation exchange resin in the hydrogen form e.g. "Amberlite" 1R-120 (Registered Trade Mark) to adsorb the PA 155A and B and then neutralising the acidic effluent by passing it through a weakly basic anion exchange resin in the hydroxyl form e.g. Amberlite 1R-4B, the final effluent being concentrated and freeze dried. PA 155A is active against gram positive and gram negative microorganisms; it is basic forming salts with both inorganic and organic acids such as hydrochloric, phosphoric, nitric, sulphuric, tartaric, glycolic, citric, glucomic, malic, maleic, succinic, glutaric, acetic, benzoic and picric acids; melting point 209-210 DEG C.; optical rotation [a ]D25= -214 DEG (C=2% methanol); contains carbon 65,7%, hydrogen 6,0%, nitrogen 16,2%, oxygen 12,1% (by difference) C-CH3 5,44%; ultraviolet absorption spectrum in methanol shows maxima at 218, 273, 281 and 288 mu with E 1% cm of 1960, 200, 233 and 186; infra red absorption spectrum shows maxima at 3.20, 3.36, 3.46, 5,82, 6.15 to 6.35, 6.67, 6.84 to 6.93, 7.08, 7.30, 7.46, 7.75, 7.86, 7.94, 8.20, 8.67, 8.91, 9.01, 9.16, 9.29, 9.53, 9.85, 10.09, 10.46, 10.78, 11.36, 11.81, 12.22, 12.81, 12.93, 13.04, 13.38, 13.42 and 13.47 to 13.56 microns. It is sparingly soluble in water, chloroform, ether, ethyl acetate and benzene, more soluble in acetone and alcohols. Specification 692,066 is referred to.ALSO:A pharmaceutical composition comprises the antibiotic PA 155A (see Group IV (b)) together with a pharmaceutical carrier. Other drugs such as antibiotics and steroids may also be present. The preparations may be in the form of tablets or liquid preparations. Specification 692,066 is referred to.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US862685XA | 1956-11-02 | 1956-11-02 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB862685A true GB862685A (en) | 1961-03-15 |
Family
ID=22197418
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB33074/57A Expired GB862685A (en) | 1956-11-02 | 1957-10-23 | New antibiotic designated pa 155a |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB862685A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997049703A2 (en) * | 1996-06-25 | 1997-12-31 | Takeda Chemical Industries, Ltd. | Oxazolone derivatives and their use as anti-helicobacter pylori agents |
US6169102B1 (en) | 1996-06-25 | 2001-01-02 | Takeda Chemical Industries, Ltd. | Oxazolone derivatives and their use as anti-Helicobacter pylori agent |
-
1957
- 1957-10-23 GB GB33074/57A patent/GB862685A/en not_active Expired
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997049703A2 (en) * | 1996-06-25 | 1997-12-31 | Takeda Chemical Industries, Ltd. | Oxazolone derivatives and their use as anti-helicobacter pylori agents |
WO1997049703A3 (en) * | 1996-06-25 | 1998-02-26 | Takeda Chemical Industries Ltd | Oxazolone derivatives and their use as anti-helicobacter pylori agents |
US6169102B1 (en) | 1996-06-25 | 2001-01-02 | Takeda Chemical Industries, Ltd. | Oxazolone derivatives and their use as anti-Helicobacter pylori agent |
US6414003B1 (en) | 1996-06-25 | 2002-07-02 | Takeda Chemical Industries, Ltd. | Oxazolone derivatives and their use as anti-helicobacter pylori agents |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
GB1395907A (en) | Antibiotics | |
GB715362A (en) | Improvements in or relating to antimicrobial agent | |
GB1058686A (en) | The antibiotic rubradirin | |
GB862685A (en) | New antibiotic designated pa 155a | |
MXPA97008774A (en) | Procedure for the manufacture of acidoclavulan salt | |
GB1349633A (en) | Antifungal agents | |
US2990329A (en) | Preparation of ristocetin a salts | |
CN108658827A (en) | A kind of preparation method of proline crude product | |
GB1038242A (en) | Bleomycin and processes for preparation thereof | |
GB1349179A (en) | Antibiotic | |
US3118812A (en) | Antibiotics lankavamycin and lankavacidin and process of production | |
SE309955B (en) | ||
US3131126A (en) | Antibiotic and process for its manufacture | |
GB901830A (en) | A new antibiotic danubomycin and a process for its manufacture | |
US3734828A (en) | Process for the separation and purification of riboflavinyl glycosides | |
US2866784A (en) | Process for obtaining voacanga alkaloids | |
CN108640865A (en) | A kind of preparation method of medicinal proline | |
GB817385A (en) | Process for the production of an antibiotic designated kanamycin | |
GB1292010A (en) | A new antibiotic, its production and its use | |
US2630401A (en) | Nitrous acid treatment for separating impurities from antipernicious anaemia active material | |
GB1356080A (en) | Antibiotic a-103-a and process for the production thereof | |
GB823115A (en) | A new antibiotic actinobolin and salts thereof | |
US3461113A (en) | Process for recovering flavin-adenine dinucleotide | |
GB1010380A (en) | Antibiotic showdomycin and a process for its production | |
GB708686A (en) | Antibiotic d-13 and its production |