GB651545A - Improvements in or relating to the preparation of enzymes - Google Patents

Improvements in or relating to the preparation of enzymes

Info

Publication number
GB651545A
GB651545A GB13866/48A GB1386648A GB651545A GB 651545 A GB651545 A GB 651545A GB 13866/48 A GB13866/48 A GB 13866/48A GB 1386648 A GB1386648 A GB 1386648A GB 651545 A GB651545 A GB 651545A
Authority
GB
United Kingdom
Prior art keywords
liquid
separating
adjusting
solid material
hyaluronidase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB13866/48A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Pharmaceutical Corp
Original Assignee
Ortho Pharmaceutical Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ortho Pharmaceutical Corp filed Critical Ortho Pharmaceutical Corp
Publication of GB651545A publication Critical patent/GB651545A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

Hyaluronidase is prepared from finely-divided mammalian testicular tissue by adding water thereto, adjusting the pH of the aqueous mixture to 4.5-4.7 (preferably 4.6), separating the solid material after prolonged stirring (preferably for at least 12 hours), adjusting the pH of the liquid to 7.5-9.2 (preferably 8.0-8.2), separating the resulting precipitate of inactive solid material, adjusting the pH of the liquid to 4.6-7.1 (preferably 4.6-5.2), adding ammonium sulphate (preferably by dialysis) in such amount that the liquid is 25-30 per cent saturated therewith, separating the further precipitate of inactive solid material, readjusting the pH of the liquid to 4.6-7.1 (preferably by dialysis) until the liquid is 60-80 per cent saturated therewith, thereby precipitating hyaluronidase, which is separated from the liquid and may be redissolved in water and the solution dialysed against running water, filtered and evaporated to dryness, the whole process being conducted at a temperature of 1-5 DEG C. The product may be further purified by alcohol fractionation or a second fractionation with ammonium sulphate. An example describes the application of the process to bull testes, which are prepared for treatment by freezing, removing the outer membrane, grinding to powder in the presence of dry ice, allowing to come to room temperature and further grinding in a Waring blender. The Specification as open to inspection under Sect. 91 comprises the use of a pH range up to 5.7 in the first step of the process. This subject-matter does not appear in the Specification as accepted.
GB13866/48A 1947-06-04 1948-05-21 Improvements in or relating to the preparation of enzymes Expired GB651545A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US651545XA 1947-06-04 1947-06-04

Publications (1)

Publication Number Publication Date
GB651545A true GB651545A (en) 1951-04-04

Family

ID=22060696

Family Applications (1)

Application Number Title Priority Date Filing Date
GB13866/48A Expired GB651545A (en) 1947-06-04 1948-05-21 Improvements in or relating to the preparation of enzymes

Country Status (1)

Country Link
GB (1) GB651545A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2795529A (en) * 1954-06-17 1957-06-11 American Home Prod Stabilized hyaluronidase solution containing calcium chloride
US2806815A (en) * 1955-04-12 1957-09-17 Ortho Pharma Corp Stabilized hyaluronidase
FR2442270A1 (en) * 1978-11-22 1980-06-20 Vnii Myasnoi Promy PROCESS FOR THE MANUFACTURE OF A DIASTASTIC PREPARATION, PARTICULARLY FOR THE MATURATION OF BUTCHERY PRODUCTS AND PRODUCTS OBTAINED BY SAID PROCESS
WO1988002261A1 (en) * 1986-09-30 1988-04-07 Biochemie Gesellschaft M.B.H. Use of hyaluronidase

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2795529A (en) * 1954-06-17 1957-06-11 American Home Prod Stabilized hyaluronidase solution containing calcium chloride
US2806815A (en) * 1955-04-12 1957-09-17 Ortho Pharma Corp Stabilized hyaluronidase
FR2442270A1 (en) * 1978-11-22 1980-06-20 Vnii Myasnoi Promy PROCESS FOR THE MANUFACTURE OF A DIASTASTIC PREPARATION, PARTICULARLY FOR THE MATURATION OF BUTCHERY PRODUCTS AND PRODUCTS OBTAINED BY SAID PROCESS
WO1988002261A1 (en) * 1986-09-30 1988-04-07 Biochemie Gesellschaft M.B.H. Use of hyaluronidase
EP0263417A1 (en) * 1986-09-30 1988-04-13 BIOCHEMIE Gesellschaft m.b.H. Use of hyaluronidase

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