GB651544A - Improvements in or relating to the preparation of enzymes - Google Patents
Improvements in or relating to the preparation of enzymesInfo
- Publication number
- GB651544A GB651544A GB13700/48A GB1370048A GB651544A GB 651544 A GB651544 A GB 651544A GB 13700/48 A GB13700/48 A GB 13700/48A GB 1370048 A GB1370048 A GB 1370048A GB 651544 A GB651544 A GB 651544A
- Authority
- GB
- United Kingdom
- Prior art keywords
- liquid
- adjusting
- solid material
- separating
- dry ice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Hyaluronidase is prepared from finely-divided mammalian testicular tissue by adding water thereto, adjusting the pH of the aqueous mixture to 4.5-4.7 (preferably 4.6), separating the solid material after prolonged stirring (preferably for at least 12 hours), adjusting the pH of the liquid to 7.5-9.2 (preferably 8.0-8.2), separating the resulting precipitate of inactive solid material, adjusting the pH of the liquid to 5.9-6.3, again separating precipitated inactive solid material, adjusting the pH of the liquid to 4.6-7.0 (preferably 4.6-4.8), adding alcohol in such amount that the volume percentage thereof in the liquid is 20-30 (preferably 27-30), separating the further precipitate of inactive solid material, and adding more ethyl alcohol to the liquid to increase the volume percentage thereof to 45-55, thereby producing a precipitate containing hyaluronidase, which is separated from the liquid and may be redissolved in water and the solution diolysed against running water, filtered and evaporated to dryness, the whole process being conducted at a temperature below 5 DEG C. An example describes the application of the process to bull testes, which are prepared for treatment by freezing in dry ice, removing the outer membrane, grinding to powder in the presence of dry ice, allowing the dry ice to evaporate and further grinding the material in a Waring blender.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US651544XA | 1947-06-03 | 1947-06-03 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB651544A true GB651544A (en) | 1951-04-04 |
Family
ID=22060694
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB13700/48A Expired GB651544A (en) | 1947-06-03 | 1948-05-20 | Improvements in or relating to the preparation of enzymes |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB651544A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103667210A (en) * | 2013-11-27 | 2014-03-26 | 青岛康原药业有限公司 | Low temperature ethanol method for extracting hyaluronidase crude product |
-
1948
- 1948-05-20 GB GB13700/48A patent/GB651544A/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103667210A (en) * | 2013-11-27 | 2014-03-26 | 青岛康原药业有限公司 | Low temperature ethanol method for extracting hyaluronidase crude product |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Roelofsen | Yeast mannan, a cell wall constituent of baker's yeast | |
US2488565A (en) | Preparation of hyaluronidase | |
SU472491A3 (en) | The method of obtaining fat and protein from plant material | |
GB651544A (en) | Improvements in or relating to the preparation of enzymes | |
GB651545A (en) | Improvements in or relating to the preparation of enzymes | |
US1892247A (en) | Process of preparing proteinases free from peptidases | |
US2542984A (en) | Method of solubilizing keratin | |
US2489173A (en) | Preparation of whipping composition and the resulting product | |
US3168448A (en) | Process of preparing a lipolytic enzyme composition | |
GB721312A (en) | Improvements in or relating to the formation of fibrous protein from solutions of collagen | |
US1541006A (en) | Process of making vegetable milk | |
US3660237A (en) | Process for obtaining kallekrein from pancreas or submandibularis glands of pigs | |
US3262854A (en) | Method for the recovery of heparin | |
FI65083C (en) | FOERFARANDE FOER SEPARERANDE AV VITALT VETEGLUTEN OCH SOLUBILISERAD VETESTAERKELSE FRAON VETEMJOEL | |
US2585546A (en) | Production of high viscosity hyaluronic acid | |
Satomura et al. | Purification and some properties of the lipase and phospholipase of Sclerotinia fungus | |
FR2072694A5 (en) | Plant extract non-dissociable by fermenta- - tion for dilution with water or alcohol | |
GB356638A (en) | Improvements in or relating to the manufacture of proteinases free from peptidases | |
GB801553A (en) | The production of edible protein products from fish solubles | |
SU66100A1 (en) | The method of obtaining dubitel | |
Bish | The determination of small quantities of starch in vegetable tissue | |
US2614063A (en) | Preparation of catalase from red blood cells and whole blood | |
US1016115A (en) | Process for rendering vegetable proteins soluble. | |
GB690685A (en) | Improvements in or relating to a process for the production of laminarin and glucose | |
GB220395A (en) | Improvements relating to the production of manures |