GB651544A - Improvements in or relating to the preparation of enzymes - Google Patents

Improvements in or relating to the preparation of enzymes

Info

Publication number
GB651544A
GB651544A GB13700/48A GB1370048A GB651544A GB 651544 A GB651544 A GB 651544A GB 13700/48 A GB13700/48 A GB 13700/48A GB 1370048 A GB1370048 A GB 1370048A GB 651544 A GB651544 A GB 651544A
Authority
GB
United Kingdom
Prior art keywords
liquid
adjusting
solid material
separating
dry ice
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB13700/48A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Pharmaceutical Corp
Original Assignee
Ortho Pharmaceutical Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ortho Pharmaceutical Corp filed Critical Ortho Pharmaceutical Corp
Publication of GB651544A publication Critical patent/GB651544A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Hyaluronidase is prepared from finely-divided mammalian testicular tissue by adding water thereto, adjusting the pH of the aqueous mixture to 4.5-4.7 (preferably 4.6), separating the solid material after prolonged stirring (preferably for at least 12 hours), adjusting the pH of the liquid to 7.5-9.2 (preferably 8.0-8.2), separating the resulting precipitate of inactive solid material, adjusting the pH of the liquid to 5.9-6.3, again separating precipitated inactive solid material, adjusting the pH of the liquid to 4.6-7.0 (preferably 4.6-4.8), adding alcohol in such amount that the volume percentage thereof in the liquid is 20-30 (preferably 27-30), separating the further precipitate of inactive solid material, and adding more ethyl alcohol to the liquid to increase the volume percentage thereof to 45-55, thereby producing a precipitate containing hyaluronidase, which is separated from the liquid and may be redissolved in water and the solution diolysed against running water, filtered and evaporated to dryness, the whole process being conducted at a temperature below 5 DEG C. An example describes the application of the process to bull testes, which are prepared for treatment by freezing in dry ice, removing the outer membrane, grinding to powder in the presence of dry ice, allowing the dry ice to evaporate and further grinding the material in a Waring blender.
GB13700/48A 1947-06-03 1948-05-20 Improvements in or relating to the preparation of enzymes Expired GB651544A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US651544XA 1947-06-03 1947-06-03

Publications (1)

Publication Number Publication Date
GB651544A true GB651544A (en) 1951-04-04

Family

ID=22060694

Family Applications (1)

Application Number Title Priority Date Filing Date
GB13700/48A Expired GB651544A (en) 1947-06-03 1948-05-20 Improvements in or relating to the preparation of enzymes

Country Status (1)

Country Link
GB (1) GB651544A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103667210A (en) * 2013-11-27 2014-03-26 青岛康原药业有限公司 Low temperature ethanol method for extracting hyaluronidase crude product

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103667210A (en) * 2013-11-27 2014-03-26 青岛康原药业有限公司 Low temperature ethanol method for extracting hyaluronidase crude product

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