GB561333A - Extraction of an enzyme substance - Google Patents
Extraction of an enzyme substanceInfo
- Publication number
- GB561333A GB561333A GB8993/42A GB899342A GB561333A GB 561333 A GB561333 A GB 561333A GB 8993/42 A GB8993/42 A GB 8993/42A GB 899342 A GB899342 A GB 899342A GB 561333 A GB561333 A GB 561333A
- Authority
- GB
- United Kingdom
- Prior art keywords
- enzyme
- concentration
- per cent
- solvent
- acetone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/37—Digestive system
- A61K35/38—Stomach; Intestine; Goblet cells; Oral mucosa; Saliva
Abstract
An enzymic material, known as erepsin, is extracted from glandular, intestinal and like animal matter, by first treating the animal matter with a water-miscible solvent boiling not above 100 DEG C. in such quantity that its concentration in the mixture is not less than 60 per cent whereby the cells are disrupted but the enzyme is not dissolved, and then treating with the same kind of solvent at a concentration of 12-20 per cent to dissolve the enzyme, for instance by incompletely removing the strong solvent and adding the requisite quantity of water. The enzyme is separated from the extract by adding enough strong solvent to bring the concentration to above 60 per cent, and the precipitated enzyme is then dried. The drying may be effected partially below 20 DEG C. and completed above that temperature. Suitable solvents are acetone and ethyl and methyl alcohols, methyl ethyl ketone and azeotropic mixtures of acetone with any of these other solvents. The initial material may be intestinal material, preferably mucosa. Specifications 561,204, [Group VI], and 561,334 are referred to.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB8993/42A GB561333A (en) | 1942-06-30 | 1942-06-30 | Extraction of an enzyme substance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB8993/42A GB561333A (en) | 1942-06-30 | 1942-06-30 | Extraction of an enzyme substance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB561333A true GB561333A (en) | 1944-05-16 |
Family
ID=9863293
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB8993/42A Expired GB561333A (en) | 1942-06-30 | 1942-06-30 | Extraction of an enzyme substance |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB561333A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4048303A (en) | 1974-11-29 | 1977-09-13 | L'opochimie | Method of preparing enzyme cholic acids complex |
-
1942
- 1942-06-30 GB GB8993/42A patent/GB561333A/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4048303A (en) | 1974-11-29 | 1977-09-13 | L'opochimie | Method of preparing enzyme cholic acids complex |
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