GB2595320A - Microscopic instrument and method for repeated location and measurement using a series of digital images - Google Patents
Microscopic instrument and method for repeated location and measurement using a series of digital images Download PDFInfo
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- GB2595320A GB2595320A GB2017918.0A GB202017918A GB2595320A GB 2595320 A GB2595320 A GB 2595320A GB 202017918 A GB202017918 A GB 202017918A GB 2595320 A GB2595320 A GB 2595320A
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/0016—Technical microscopes, e.g. for inspection or measuring in industrial production processes
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/32—Micromanipulators structurally combined with microscopes
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
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- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Engineering & Computer Science (AREA)
- Multimedia (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Microscoopes, Condenser (AREA)
Abstract
A microscopic instrument 200 includes a group 110 of imaging objective lenses 111,112 of different focal lengths in series. At least one optical splitter 130 is placed between the lenses splitting the light beam into optical channels. Multiple photoelectric imaging devices 141,142, are arranged with each one in the focal plane of an optical channel corresponding to the photoelectric imaging device. The component parts may be fixed by connection to a first mechanical structure forming a first integral structure. Microscopic observation relocates the microscopic instrument and observes an object repeatedly by using a series of previously acquired digital images. This is accomplished by placing an object to be measured on a workstation, adjusting a direction of an incident ray to one optical lens of a group of imaging objective lenses toward the object and generating an observation image using an electrical signal converted by each of the photoelectric imaging devices. A driving mechanism is controlled to perform multi-dimensional movement according to a series of digital images, generated previously, until the observation image and the series of digital images generated previously are matched with each other, so as to relocate the microscopic instrument and observe the object repeatedly.
Description
MICROSCOPIC INSTRUMENT AND METHOD FOR REPEATED LOCATION
AND MEASUREMENT USING A SERIES OF DIGITAL IMAGES
CROSS-REFERENCE TO RELATED APPLICATION(S)
This application claims priority to the Chinese Patent Application No. 202010438742.8, filed on May 21, 2020.
TECHNICAL FIELD
The present disclosure relates to the technical field of electronics, in particular to a microscopic instrument and a method for repeated location and measurement using a series of digital images.
BACKGROUND
In general, human eyes cannot directly observe structural details of objects smaller than 0.1mm. In order to observe smaller physical structures, people usually need to use a microscopic magnification instrument.
A microscope is an instrument that produces magnified images of objects by means of physical methods. With the development of industry and technology, improvement of the instrument can increase a magnification and an ability to distinguish fine structures, which enables the microscope to be widely used in various industrial and scientific research activities such as biology, chemistry, physics, metallurgy and brewing, and makes great and outstanding contribution to development of human beings.
SUMMARY
The present disclosure provides a microscopic instrument and a method for repeated location and measurement using a series of digital images.
An aspect of the present disclosure provides a microscopic instrument, comprising: a group of imaging objective lens, including a plurality of optical lenses with different focal lengths arranged in series along a direction of their optical axis; at least one optical splitter, arranged between the plurality of optical lenses with different focal lengths, and configured to split a light beam received by the group of imaging objective lens into a plurality of optical channels; and a plurality of photoelectric imaging devices, configured to convert an optical signal output from the at least one optical splitter to an electrical signal, wherein each of the plurality of photoelectric imaging devices is arranged in a focal plane of each optical channel corresponding to the photoelectric imaging device.
According to an embodiment of the present disclosure, the group of imaging objective lens comprises a first optical lens with a first focal length and a second optical lens with a second focal length; wherein the at least one optical splitter is arranged between the first optical lens and the second optical lens; and wherein the plurality of photoelectric imaging devices comprises a first photoelectric imaging device and a second photoelectric imaging device, wherein the first photoelectric imaging device is arranged in a focal plane of a first optical channel, and the second photoelectric imaging device is arranged in a focal plane of a second optical channel.
According to an embodiment of the present disclosure, the plurality of photoelectric imaging devices, the group of imaging objective lens and the at least one optical splitter are fixedly connected by a first mechanical structure to form a first integral structure.
According to an embodiment of the present disclosure, the microscopic instrument further comprises a driving mechanism, having a multi-dimensional adjustment function, configured to drive the first integral structure, wherein the driving mechanism and the first integral structure are connected by a second mechanical structure.
According to an embodiment of the present disclosure, the microscopic instrument further comprises an electronic control device, configured to generate a series of digital image files according to the electrical signal converted by each of the plurality of photoelectric imaging devices, wherein the series of digital image files comprises positional information in relation with the series of digital image files.
According to an embodiment of the present disclosure, the electronic control device is further configured to control the driving mechanism to perform multi-dimensional movement according to a series of digital image files generated previously, so as to locate the microscopic instrument and observe an object repeatedly.
According to an embodiment of the present disclosure, the microscopic instrument further comprises a storage medium, configured to store the series of digital image files.
According to an embodiment of the present disclosure, the at least one optical splitter comprises a first optical splitter and a second optical splitter; wherein the plurality of photoelectric imaging devices further comprise a third photoelectric imaging device, and the third photoelectric imaging device is arranged in a focal plane of a third optical channel; wherein a third optical lens is further arranged between the third photoelectric imaging device and the second optical splitter.
According to an embodiment of the present disclosure, the plurality of photoelectric imaging devices and the optical channels are arranged one by one.
Another aspect of the present disclosure provides a method for microscopic observation, used to relocate a microscopic instrument and observe an object repeatedly by using a series of digital images acquired by the microscopic instrument according to the present disclosure, comprising: placing an object to be measured on a workstation; adjusting a direction of incident ray to one optical lens of a group of imaging objective lens toward the object to be measured; generating an observation image using an electrical signal converted by each of a plurality of photoelectric imaging devices; and controlling, by an electronic control device of the microscopic instrument, a driving mechanism to perform multi-dimensional movement according to a series of digital images generated previously, until the observation image and the series of digital images generated previously are matched with each other, so as to relocate the microscopic instrument and observe the object repeatedly.
BRIEF DESCRIPTION OF THE ACCOMPANYING DRAWINGS
The above and other objects, features and advantages of the present disclosure will be clearer through the following description of embodiments of the present disclosure with reference to the accompanying drawings, in which: Fig. 1 illustrates a schematic diagram of composition of an imaging optical path of a microscopic instrument according to an embodiment of the present disclosure; Fig. 2 illustrates a schematic diagram of a microscopic instrument comprising a driving mechanism according to an embodiment of the present disclosure; Fig. 3 illustrates a schematic diagram of a microscopic instrument comprising a driving mechanism and an electronic control device according to an embodiment of the present disclosure; Fig. 4 illustrates a schematic diagram of the electronic control device controlling the driving mechanism in a wireless mode according to an embodiment of the present disclosure; and Fig. 5 illustrates a schematic diagram of a microscopic instrument according to another embodiment of the present disclosure.
DETAILED DESCRIPTION
Hereinafter, embodiments of the present disclosure will be described with reference to the accompanying drawings. However, it should be understood that these descriptions are only exemplary and are not intended to limit the scope of the present disclosure. In the following detailed description, for ease of explanation, many specific details are set forth to provide a comprehensive understanding of the embodiments of the present disclosure. However, it is obvious that one or more embodiments may also be implemented without these specific details. In addition, in the following description, descriptions of well-known structures and techniques are omitted to avoid unnecessarily obscuring the concepts of the present disclosure.
The terms used herein are for describing specific embodiments only, and are not intended to limit the present disclosure. The terms "comprising", "including", and the like used herein indicate the presence of the described features, steps, operations, and/or components, but do not exclude the presence or addition of one or more other features, steps, operations, or components.
All terms (including technical and scientific terms) used herein have the meaning commonly understood by those skilled in the art unless otherwise defined. It should be noted that the terms used herein should be interpreted as having a meaning consistent with the context of this specification, and should not be interpreted in an idealized or overly stereotypical manner.
With rapid development of digital imaging technology, computers are used to process microscopic images, which makes the way for observing and analyzing microscopic images by people one step forward.
In the field of industrial microscopic measurement, an object distance of a conventional optical microscope is relatively short, and the number of objective lenses is generally single. It is necessary to make an optical front end of the microscope close to a target object, and observation and measurement for local fine structure of the target object are performed by magnifying an image of the target. As a result, an application of microscopes will be restricted, especially in certain environments where close observation is not possible. Furthermore, because the microscope is an image magnification and observation instrument, affected by a magnification effect, in the process of the measurement and location using a magnified image of the target, especially when it is necessary to relocate at local details of the target object for reproduction of microscopic details and multiple repeated location and measurement, higher requirements are placed on location and adjustment accuracy of the microscope and its adjustment efficiency during repeated observation. However, it is difficult for existing conventional microscope apparatus to meet the requirements of adjustment accuracy and adjustment efficiency for relocation and repeated observation directly.
In order to at least partially solve the technical problem that the conventional microscope needs to be close to the object to be observed due to the short working object distance, which results in limited application environment, or the technical problem existing in the conventional microscope that it is difficult to realize high-precision relocation of the microscope and reproduction of the microscopic details and rapid repeated measurement, an embodiment of the present disclosure provides a novel microscopic instrument.
The microscopic instrument provided by the embodiment of the present disclosure comprises: a group of imaging objective lens, including a plurality of optical lenses with different focal lengths arranged in series along a direction of their optical axis; at least one optical splitter, arranged between the plurality of optical lenses with different focal lengths, and configured to split a light beam received by the group of imaging objective lens into a plurality of optical channels; and a plurality of photoelectric imaging devices, configured to convert an optical signal output from the at least one optical splitter to an electrical signal, and each of the plurality of the photoelectric imaging devices is arranged in a focal plane of the optical channel corresponding to the photoelectric imaging device.
Fig. 1 illustrates a schematic diagram of composition of an imaging optical path of a microscopic instrument according to an embodiment of the present disclosure.
As shown in Fig. 1, a microscopic instrument 100 comprises a group of imaging objective lens 100, including a plurality of optical lenses with different focal lengths arranged in series along a direction of their optical axis. For example, the group of imaging objective lens 100 comprises a first optical lens 111 with a first focal length and a second optical lens 112 with a second focal length.
According to the embodiment of the present disclosure, the first focal length of the first optical lens 111 may be greater than the second focal length of the second optical lens 112, so that when an object 120 is observed, there is no need to place an optical front end of the microscopic instrument 100 too close to the object 120 compared with the prior art. The first focal length of the first optical lens 111 may be, for example, 100 mm, and the second focal length of the second optical lens 112 may be, for example, 10 mm. Of course, the focal length of the optical lens in the embodiment of the present disclosure is not limited to this. Design parameters of the first optical lens 111 and the second optical lens 112 may be determined according to the microscopic observation needs of the object to be measured.
According to the embodiment of the present disclosure, the focal lengths of the plurality of optical lenses may be continuously reduced along the direction of the optical axis, and the direction of the optical axis may be based on the optical axis of the first optical lens 111 itself.
According to the embodiment of the present disclosure, the microscopic instrument 100 further comprises an optical splitter 130, configured to split a light beam received by the group of imaging objective lens into a plurality of optical channels, and arranged between the plurality of optical lenses. For example, the optical splitter 130 may be arranged between the first optical lens 111 and the second optical lens 112 to split the light beam received by the first optical lens 111 close to the object to be measured into a plurality of optical channels. Among them, an optical signal reflected by the optical splitter 130 may be directed to a first photoelectric imaging device 141, and then collected by the first photoelectric imaging device 141. Herein, the channel formed when the optical signal reflected by the optical splitter 130 is directed to the first photoelectric imaging device 141 may act as an optical channel. The optical signal transmitted through the optical splitter 130 may be directed to the second optical lens 112, then pass through the second optical lens 112 and be collected by a second photoelectric imaging device 142. Herein, the channel formed when the optical signal transmitted through the optical splitter 130 is directed to the second imaging objective lens 112, passes through the second optical lens 112 and is directed to the second photoelectric imaging device 142 may act as another optical channel.
According to the embodiment of the present disclosure, the optical splitter 130 may adopt an optical beam splitting prism or an optical beam splitter, which is determined by application requirements of a specific working environment. Through forming a plurality of optical channels capable of imaging synchronously by the optical splitter, no less than two optical images will be synchronously formed for the same target object, and each of various optical images has different optical magnifications.
According to the embodiment of the present disclosure, the microscopic instrument 100 further comprises a plurality of photoelectric imaging devices 140, configured to convert an optical signal output from the optical splitter 130 to an electrical signal, and each of the plurality of the photoelectric imaging devices 140 is arranged in a focal plane of the optical channels corresponding to the photoelectric imaging device. According to the embodiment of the present disclosure, the photoelectric imaging devices and the optical channels are arranged one by one.
For example, the plurality of photoelectric imaging devices 140 comprise a first photoelectric imaging device 141 and a second photoelectric imaging device 142. The first photoelectric imaging device 141 is arranged in the focal plane of the first optical channel, and the second photoelectric imaging device 142 is arranged in the focal plane of the second optical channel.
Since the first optical lens 111 and the second optical lens 112 have different focal lengths, synchronous observation of the local fine structure of the object 120 with different magnifications and synchronous recording of the series of corresponding digital images are realized by the optical splitter 130, the first photoelectric imaging device 141 and the second photoelectric imaging device 142.
According to the embodiment of the present disclosure, each of the photoelectric imaging devices may comprise a photoelectric imaging device and a related imaging circuit. Each of the optical channels would be provided with a photoelectronic imaging device in the focal plane of the optical channel, so as to synchronously acquire optical images with different magnifications for each of the optical channels. For example, the optical image under the first focal length of the first optical lens 111 may be acquired by the photoelectric imaging device 141, and the second focal length of the second optical lens 112 may be acquired by the photoelectric imaging device 142.
According to the embodiment of the present disclosure, each of the optical channels is formed from transmission of the optical signals after arrangements of the imaging objective lens and the optical splitter are determined.
According to an embodiment of the present disclosure, there is further provided a method for repeated location and measurement using a series of digital images, comprising: placing an object to be measured on a workstation; adjusting a direction of incident light toward the first optical lens of the group of imaging objective lens to make sure that the object to be measured; and generating a series of observational digital images using the electrical signal converted by each of the photoelectric imaging devices. According to the embodiment of the present disclosure, the series of digital images may be displayed on a screen. The series of digital images generated by the electrical signals converted by each of the photoelectric imaging devices may be displayed separately or totally In the microscopic instrument according to the embodiment of the present disclosure, the microscopic instrument comprises a group of imaging objective lens including a plurality of optical lenses with different focal lengths arranged in series along a direction of their optical axis. By arranging the optical splitter between the plurality of optical lenses with different focal lengths, the optical splitter will split incident light received by the group of imaging objective lens into a plurality of optical channels. In the case of a plurality of optical lenses with different focal lengths, an optical signal is converted into an electrical signal by a corresponding photoelectric imaging device, which can realize synchronous observation of multi-channel micro images with different magnifications of the local fine structure of the object to be observed and the synchronous recording of corresponding sequence digital images. In addition, the instrument has a long working object distance, which can meet the needs of relatively long-distance observation when close observation is not possible. Therefore, it at least partially overcomes the technical problem that the conventional microscope needs to be close to the object to be observed due to a short working object distance, which results in limited application environment. Therefore, the microscopic instrument has a wider application environment, and achieves synchronous observation of multi-channel micro images with different magnifications of the local fine structure of the object to be observed and synchronous recording of the corresponding sequence digital images.
According to the embodiment of the present disclosure, the first photoelectric imaging device 141 and the first optical lens 111, the optical splitter130, and the second photoelectric imaging device 142 and the second optical lens 112 may be fixedly connected by a first mechanical structure to form a first integral structure.
According to the embodiment of the present disclosure, the fixed connection by the first mechanical structure may be, for example, a fixation by a structure connection fitting. According to the embodiment of the present disclosure, the microscopic instrument may further comprise a driving mechanism 150. According to the embodiment of the present disclosure, a first integral structure may be placed on the driving mechanism 150 with a multi-dimensional adjustment function, and the driving mechanism may translate and rotate in three orthogonal directions.
Fig. 2 illustrates a schematic diagram of a microscopic instrument comprising a driving mechanism according to an embodiment of the present disclosure.
As shown in Fig. 2, a microscopic instrument 100' according to the embodiment of the present application further comprises a driving mechanism 150 in addition to the group of imaging objective lenses 110, the optical splitter 130 and the plurality of photoelectric imaging devices 140 shown in Fig. 1. According to the embodiment of the present disclosure, the driving mechanism 150 may be arranged on a base of the microscopic instrument 100'. The driving mechanism 150 may translate and rotate in three orthogonal directions to adjust the first integral structure, so as to facilitate observation of the object 120 to be measured. According to the embodiment of the present disclosure, it is possible to drive the overall movement of the optical multi-channel microscopic imaging lenses and the photoelectric imaging devices. Through the driving of the driving mechanism 150, adjustment of the microscopic observation position of the object to be measured and adjustment of the clarity of the microscopic image can be achieved.
According to the embodiment of the present disclosure, the driving mechanism 150 may be implemented by manual control or electric control. An adjustment control way, a driving step and a driving range of the geometric adjustment, and a driving accuracy and a driving speed of the driving mechanism may be determined by application requirements of a specific working environment and correlation parameters of the series of digital images, which are captured synchronously by the group of imaging objective lens 110.
Fig. 3 illustrates a schematic diagram of a microscopic instrument comprising a driving mechanism and an electronic control device according to an embodiment of the present disclosure.
As shown in Fig. 3, in addition to the group of imaging objective lenses 110, the optical splitter 130, the plurality of photoelectric imaging devices 140, and the driving mechanism 150 shown in Fig. 2, a microscopic instrument 200 may further comprise an electronic control device 160, configured to generate a series of digital images according to the electrical signal converted by each of the photoelectric imaging devices, wherein the series of digital images comprise positional information in relation with the digital images.
According to the embodiment of the present disclosure, the electronic control device 160 may display, collect and record a series of digital images outputted by the photoelectric imaging devices 140 in real time, and store the series of digital images and their correlation parameters which would be the positional information in relation with the series of digital images. The electronic control device 160 also can read and display in parallel the series of digital images saved earlier. The series of digital images saved previously may be used as a reference for the adjustment of the microscopic observation to control a multi-dimensional movement of the driving mechanism 150, so as to relocate the microscopic instrument and observe the local fine structure of the object repeatedly.
According to the embodiment of the present disclosure, the electric control device 160 may be used for displaying the digital images. The electric control device 160 may comprise an input/output port and a display module, a power supply and a driving circuit module, and related management and operation software According to the embodiment of the present disclosure, the microscopic instrument 200 may further comprise a storage medium for storing the series of digital images. The electronic control device 160 may access the storage medium for storing the series of digital images.
According to the embodiment of the present disclosure, the input/output port and the display module can synchronously collect and display optical images acquired with different magnifications of each of the optical channels in real time, and may convert real-time microscopic images into a series of storable digital images according to control instructions. Further, the series of digital image files may be stored in the storage medium.
In addition, the electronic control device 160 may also read the series of digital images from the microscopic instrument itself or other electronic mobile storage medium, and display the digital images through the display module.
According to the embodiment of the present disclosure, the power supply and the driving circuit module, and the related management and operation software, can supply power to the microscopic instrument 200 and control operation of the microscopic instrument 200, so as to acquire the series of digital images, the precise multi-dimensional adjustment of the optical multi-channel microscopic imaging objective lens, and achieve other functions. In particular, according to the series of digital images that have been read and displayed by the display module, it is possible to determine the positional information in relation to the series of digital images recorded in the series of digital image files by using manual visual measurement mode or computer automatic image recognition mode, and adjust the photoelectric imaging devices and the optical lenses by controlling the driving mechanism 150 to perform multi-dimensional movement, so as to quickly locate at some specific area repeatedly to reproduce and measure the microscopic details of the object 200 multiple times.
According to the embodiment of the present disclosure, the electric control device 160 would be used to control the driving mechanism 150 to perform multi-dimensional movement according to a series of digital images generated previously, so as to relocate the microscopic instrument and observe the object repeatedly.
According to the embodiment of the present disclosure, the electric control device 160 may perform a remote control operation in a wired or a wireless mode, so as to control the driving mechanism 150 to perform multi-dimensional movement.
In the embodiment shown in Fig. 3, the electric control device 160 performs the remote control operation in a wired mode, so as to control the driving mechanism 150 to perform multi-dimensional movement.
In the embodiment shown in Fig. 3, the object 120 is imaged by the optical lens 111, and the optical imaging light path is split into two optical channels by the optical splitter 130. Herein, the imaging light of one optical channel is received by the photoelectric imaging device 141, and the imaging light of the other optical channel is further imaged by the optical lens 112 and then received by the photoelectric imaging device 142. The photoelectric imaging device 141 and the photoelectric imaging device 142 may convert the optical signal into an electrical signal, then convert the electrical signal into a series of digital images, which are displayed on the electric control device 160 in real time. Moreover, the various components included by the microscopic instrument 200 constitute an integral structure, which is installed on the multi-dimensional driving structure 150 for precise adjustment.
Fig. 4 illustrates a schematic diagram of the electronic control device controlling the driving mechanism in a wireless mode according to an embodiment of the present disclosure. According to the embodiment of the present disclosure, the driving mechanism 150 may be a high-precision multi-dimensional adjustment mechanism. After the object is observed by a microscopic instrument 200' for the first time and then separated from the microscopic instrument, and when a further microscopic observation of the object 120 is needed again, a series of sequence images collected and stored by the microscopic instrument 200' in the first observation would be used as a reference for adjustment (that is, the driving mechanism may be controlled to perform multi-dimensional movement according to the series of digital images generated previously), so that the microscopic instrument 200' can be quickly located at an observation position in the first time and reproduce a repeated microscopic observation. A microscopic local portion of the object 120 in the first observation may be relocated accurately, and the microscopic details acquired in the first observation may be reproduced by the instrument. In this way, it is possible to solve the technical problem that the conventional microscope is difficult to be relocated accurately to quickly reproduce the microscopic details.
Fig. 5 illustrates a schematic diagram of a microscopic instrument according to another embodiment of the present disclosure. As shown in Fig. 5, a microscopic instrument 300 comprises a group of imaging objective lenses 310, a group of optical splitters 330, a plurality of photoelectric imaging devices 340, a driving mechanism 350, and an electronic control device 360.
According to the embodiment of the present disclosure, in addition to a first optical lens 311 and a second optical lens 312 arranged in series along a direction of their optical axis, the group of imaging objective lens 310 may further comprise a third optical lens 313.
According to the embodiment of the present disclosure, the group of optical splitters 330 comprises a first optical splitter 331 and a second optical splitter 332.
According to the embodiment of the present disclosure, in addition to a first photoelectric imaging device 341 and a second photoelectric imaging device 342, the plurality of photoelectric imaging devices 340 further comprise a third photoelectric imaging device 343. The third photoelectric imaging device 343 is arranged in a focal plane of a third optical channel. The third imaging objective lens 313 is arranged between the third photoelectric imaging device 343 and the second optical splitter 332.
According to the embodiment of the present disclosure, the first optical lens 311 and the second optical lens 312 may be arranged in series along a direction of their optical axis, and the third optical lens 313 may be arranged along a direction of the optical axis of the reflected ray of the second optical splitter 332.
According to the embodiment of the present disclosure, an installation position of the first optical lens 311 matches with a position of the first optical splitter 331, and an installation position of the third optical lens 313 matches with a position of the second optical splitter 332. A reflected ray which reflects from the first optical splitter 331 may be imaged with the first optical lens 311, and a transmitted ray which transmits through the first optical splitter 331 is split by the second optical splitter 332. A reflected ray which reflects from the second optical splitter 332 may be imaged with the third optical lens 313, and a transmitted ray which transmits through the second optical splitter 332 may be imaged with the second optical lens 312.
According to the embodiment of the present disclosure, after imaging light from an object 320 to be observed passes through the first optical lens 311, the imaging light is split by the first optical splitter 331 into two optical channels of reflection and transmission, wherein the reflected ray reflected by the first optical splitter 331 is directly imaged, and the transmitted ray passing through the first optical splitter 331 is split by the second optical splitter 332 into two optical channels of reflection and transmission, the reflected ray and the transmitted ray of these two channels pass through the second optical lens 313 and the third optical lens 312 respectively and then imaged separately. The images of these three channels are received and converted into digital images by respective photoelectric imaging devices, and then displayed on the electronic control device 360 in real time.
For example, an imaging optical path of the first optical lens 311 is split by the first optical splitter 331, and the reflected ray is received and converted into a digital image by the first photoelectric imaging device 341. The transmitted ray passing through the first optical splitter 331 is split by the second optical splitter 332, and the reflected ray is imaged by the third optical lens 313 and then received and converted into a digital image by the third photoelectric imaging device 343. The transmitted ray passing through the second optical splitter 332 is imaged by the second optical lens 312 and then received and converted into a digital image by the second photoelectric imaging device 342.
Moreover, the various components included in the microscopic instrument 300 form an integral structure, which is installed on the multi-dimensional driving mechanism 350 for precise location adjustment.
According to the embodiment of the present disclosure, the electronic control device 360 may perform a remote control operation in a wired mode or a wireless mode, so as to control the driving mechanism 350 to perform multi-dimensional movement.
Specifically, for example, Fig. 5 illustrates a schematic diagram of the electronic control device controlling the driving mechanism in a wireless mode according to the embodiment of the present disclosure.
The present disclosure further provides a storage medium, which may be included in the microscopic instrument described in the above embodiments, or which may exist alone without being assembled into the microscopic instrument. The storage medium carries one or more programs that, when executed, perform the method according to the embodiments of the present disclosure.
According to the embodiment of the present disclosure, the storage medium may be a non-volatile computer-readable storage medium. Examples include, but are not limited to: portable computer disks, hard disks, random access memory (RAM), read only memory (ROM), erasable programmable read only memory (EPROM or flash memory), portable compact disk read only memory (CD-ROM), optical storage device, magnetic storage device, or any suitable combination thereof. In the present disclosure, the storage medium may be any tangible medium containing or storing a program that may be used by or in combination with an instruction execution system, instrument, or device.
For example, according to the embodiment of the present disclosure, the storage medium may include ROM and/or RAM and/or one or more memories other than ROM and RAM.
Those skilled in the art may understand that the features recited in the various embodiments and/or claims of the present disclosure may be combined in various ways, even if such combinations are not explicitly described in the present disclosure. In particular, the features recited in the various embodiments and/or claims of the present disclosure may be combined in various ways without departing from the spirit and teachings of the present disclosure. All of these combinations fall within the scope of the present disclosure.
The embodiments of the present disclosure have been described above. However, these embodiments are for illustrative purposes only, and are not intended to limit the scope of the present disclosure. Although the embodiments have been described separately above, this does not mean that the measures in the various embodiments cannot be advantageously used in combination. The scope of the present disclosure is defined by the appended claims and their equivalents. Without departing from the scope of the present disclosure, those skilled in the art may make various substitutions and modifications, and these substitutions and modifications shall fall within the scope of the present disclosure.
Claims (10)
- Claims INVe claim: 1. A microscopic instrument, comprising: a group of imaging objective lens, including a plurality of optical lenses with different focal lengths arranged in series along a direction of their optical axis; at least one optical splitter, arranged between the plurality of optical lenses with different focal lengths, and configured to split a light beam received by the group of imaging objective lens into a plurality of optical channels; and a plurality of photoelectric imaging devices, configured to convert an optical signal output from the at least one optical splitter to an electrical signal, wherein each of the plurality of photoelectric imaging devices is arranged in a focal plane of each optical channel corresponding to the photoelectric imaging device.
- 2. The microscopic instrument according to claim 1, wherein, the group of imaging objective lens comprises a first optical lens with a first focal length and a second optical lens with a second focal length; wherein the at least one optical splitter is arranged between the first optical lens and the second optical lens; and wherein the plurality of photoelectric imaging devices comprises a first photoelectric imaging device and a second photoelectric imaging device, wherein the first photoelectric imaging device is arranged in a focal plane of a first optical channel, and the second photoelectric imaging device is arranged in a focal plane of a second optical channel.
- 3. The microscopic instrument according to claim 1, wherein, the plurality of photoelectric imaging devices, the group of imaging objective lens and the at least one optical splitter are fixedly connected by a first mechanical structure to form a first integral structure.IX
- 4. The microscopic instrument according to claim 3, further comprising: a driving mechanism, having a multi-dimensional adjustment function, configured to drive the first integral structure, wherein the driving mechanism and the first integral structure are connected by a second mechanical structure.
- 5. The microscopic instrument according to claim 2 or 4, further comprising: an electronic control device, configured to generate a series of digital image files according to the electrical signal converted by each of the plurality of photoelectric imaging devices, wherein the series of digital image files comprises positional information in relation with the series of digital image files.
- 6. The microscopic instrument according to claim 5, wherein, the electronic control device is further configured to control the driving mechanism to perform multi-dimensional movement according to a series of digital image files generated previously, so as to locate the microscopic instrument and observe an object repeatedly.
- 7. The microscopic instrument according to claim 5, further comprising: a storage medium, configured to store the series of digital image files.
- 8. The microscopic instrument according to claim 2, wherein, the at least one optical splitter comprises a first optical splitter and a second optical splitter; wherein the plurality of photoelectric imaging devices further comprise a third photoelectric imaging device, and the third photoelectric imaging device is arranged in a focal plane of a third optical channel; wherein a third optical lens is further arranged between the third photoelectric imaging device and the second optical splitter.
- 9. The microscopic instrument according to claim 1, wherein, the plurality of photoelectric imaging devices and the optical channels are arranged one by one.
- 10. A method for microscopic observation, used to relocate a microscopic instrument and observe an object repeatedly by using a series of digital images acquired by the microscopic instrument according to any one of claims 1 to 9, comprising: placing an object to be measured on a workstation; adjusting a direction of incident ray to one optical lens of a group of imaging objective lens toward the object to be measured; generating an observation image using an electrical signal converted by each of a plurality of photoelectric imaging devices; and controlling, by an electronic control device of the microscopic instrument, a driving mechanism to perform multi-dimensional movement according to a series of digital images generated previously, until the observation image and the series of digital images generated previously are matched with each other, so as to relocate the microscopic instrument and observe the object repeatedly.
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| CN202010438742.8A CN111505819B (en) | 2020-05-21 | 2020-05-21 | Microscopy instrument and method for repeated positioning and measurement by using digital sequence image |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020044346A1 (en) * | 2000-01-21 | 2002-04-18 | Nguyen Lam K. | Multiparallel three dimensional optical microscopy system |
| US6433876B1 (en) * | 1999-06-01 | 2002-08-13 | Optical Perspectives Group, L.L.C. | Multiple wavelength or multiple shear distance quantitative differential interference contrast microscopy |
| US6640014B1 (en) * | 1999-01-22 | 2003-10-28 | Jeffrey H. Price | Automatic on-the-fly focusing for continuous image acquisition in high-resolution microscopy |
| US20090103792A1 (en) * | 2007-10-22 | 2009-04-23 | Visiongate, Inc. | Depth of Field Extension for Optical Tomography |
| WO2014009859A2 (en) * | 2012-07-10 | 2014-01-16 | Aïmago S.A. | Perfusion assessment multi-modality optical medical device |
| KR20160090552A (en) * | 2015-01-22 | 2016-08-01 | (주)프로옵틱스 | Three-dimensional measuring apparatus has a high-precision measurement function |
| US20180196246A1 (en) * | 2016-10-19 | 2018-07-12 | Cornell University | Hyperspectral multiphoton microscope for biomedical applications |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003131142A (en) * | 2001-10-25 | 2003-05-08 | Olympus Optical Co Ltd | Microscope |
| JP4825171B2 (en) * | 2007-06-08 | 2011-11-30 | 株式会社新川 | Imaging apparatus for bonding apparatus and imaging method |
| US8054558B2 (en) * | 2009-02-11 | 2011-11-08 | Omniprobe, Inc. | Multiple magnification optical system with single objective lens |
| CN202145246U (en) * | 2011-05-17 | 2012-02-15 | 桂林市迈特光学仪器有限公司 | Double multiplying power double working distance video microscope |
| CN103955050B (en) * | 2014-05-13 | 2016-08-24 | 杨征 | A kind of multi-pass microscopic system |
| CN204331133U (en) * | 2014-12-25 | 2015-05-13 | 中国工程物理研究院机械制造工艺研究所 | High low power confocal compound microscope illuminance of image plane coalignment |
| CN204694914U (en) * | 2015-05-30 | 2015-10-07 | 东莞市兆丰精密仪器有限公司 | A kind of double-view field combined optical imaging system |
| DE102017214189A1 (en) * | 2017-08-15 | 2019-02-21 | Carl Zeiss Microscopy Gmbh | Method for operating a microscope assembly and a microscope assembly with a first microscope and at least one further microscope |
| CN108414086A (en) * | 2018-05-11 | 2018-08-17 | 中国科学院西安光学精密机械研究所 | Snapshot type microscopic hyperspectral imaging system and method |
| CN108845406A (en) * | 2018-06-06 | 2018-11-20 | 迪瑞医疗科技股份有限公司 | The full-automatic micro imaging method of more multiplying powers and device |
| CN110455799B (en) * | 2019-07-23 | 2022-06-17 | 长春理工大学 | High-resolution holographic microscope and method for live cell imaging |
-
2020
- 2020-05-21 CN CN202010438742.8A patent/CN111505819B/en active Active
- 2020-11-13 GB GB2017918.0A patent/GB2595320B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6640014B1 (en) * | 1999-01-22 | 2003-10-28 | Jeffrey H. Price | Automatic on-the-fly focusing for continuous image acquisition in high-resolution microscopy |
| US6433876B1 (en) * | 1999-06-01 | 2002-08-13 | Optical Perspectives Group, L.L.C. | Multiple wavelength or multiple shear distance quantitative differential interference contrast microscopy |
| US20020044346A1 (en) * | 2000-01-21 | 2002-04-18 | Nguyen Lam K. | Multiparallel three dimensional optical microscopy system |
| US20090103792A1 (en) * | 2007-10-22 | 2009-04-23 | Visiongate, Inc. | Depth of Field Extension for Optical Tomography |
| WO2014009859A2 (en) * | 2012-07-10 | 2014-01-16 | Aïmago S.A. | Perfusion assessment multi-modality optical medical device |
| KR20160090552A (en) * | 2015-01-22 | 2016-08-01 | (주)프로옵틱스 | Three-dimensional measuring apparatus has a high-precision measurement function |
| US20180196246A1 (en) * | 2016-10-19 | 2018-07-12 | Cornell University | Hyperspectral multiphoton microscope for biomedical applications |
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| CN111505819A (en) | 2020-08-07 |
| GB202017918D0 (en) | 2020-12-30 |
| GB2595320B (en) | 2022-08-24 |
| CN111505819B (en) | 2022-04-05 |
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