CN108414086A - A kind of snapshot-type high microspectrum imaging system and imaging method - Google Patents
A kind of snapshot-type high microspectrum imaging system and imaging method Download PDFInfo
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- CN108414086A CN108414086A CN201810450575.1A CN201810450575A CN108414086A CN 108414086 A CN108414086 A CN 108414086A CN 201810450575 A CN201810450575 A CN 201810450575A CN 108414086 A CN108414086 A CN 108414086A
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- 238000003384 imaging method Methods 0.000 title claims abstract description 77
- 238000002381 microspectrum Methods 0.000 title claims abstract description 24
- 239000006185 dispersion Substances 0.000 claims abstract description 17
- 238000001228 spectrum Methods 0.000 claims description 9
- 230000005540 biological transmission Effects 0.000 claims description 5
- 238000003491 array Methods 0.000 claims description 4
- 239000011159 matrix material Substances 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 2
- 238000005286 illumination Methods 0.000 abstract description 5
- 230000003595 spectral effect Effects 0.000 description 14
- 238000000034 method Methods 0.000 description 10
- 230000003287 optical effect Effects 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 3
- 230000003321 amplification Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000004973 liquid crystal related substance Substances 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 101100117236 Drosophila melanogaster speck gene Proteins 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000000701 chemical imaging Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000013500 data storage Methods 0.000 description 1
- 238000013135 deep learning Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012634 optical imaging Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/2823—Imaging spectrometer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/255—Details, e.g. use of specially adapted sources, lighting or optical systems
Abstract
The present invention relates to a kind of snapshot-type high microspectrum imaging system and imaging methods, solve existing microspectrum imaging technique and need time dimension scanning or space dimension scanning, it is difficult to realize the quick obtaining and imaging system complex structure and other problems of target three-dimensional information.The imaging system includes lighting system, objective table, microcobjective, beam splitter, computer, acquisition system and compensation system；Lighting system is used to carry out Uniform Illumination to the target to be seen on objective table；Beam splitter is divided the light beam by microcobjective by certain splitting ratio；Acquisition system includes the first imaging lens being successively set on beam splitter transmitted light path, microlens array, collimating mirror, dispersion element, the second imaging lens and the first detector；Compensation system includes the third imaging lens and the second detector being successively set on beam splitter reflected light path；First detector and the second detector are connect with computer respectively.
Description
Technical field
The present invention relates to optical image technology fields, and in particular to a kind of snapshot-type high microspectrum imaging system and imaging
Method.
Background technology
Microscope is 20th century of the mankind one of greatest contrivance, is frequently used to observe small object, generally quilt
Applied to biology, histology, bacteriology, pharmaceutical chemistry, medical research and clinical examination.Due to traditional micro-imaging technique
The Morphometrology for being observed object can only be provided, the constituent information of substance, therefore this imaging side cannot be further provided
Method has been unable to meet the application development requirement of micro-imaging field, and how to provide determining for the chemistry or biochemical composition for being observed sample
Amount distributed image and its change information become the hot spot of current microtechnic research.Microspectrum imaging technique is existing aobvious
On the basis of micro- imaging technique, in conjunction with a kind of novel optical imaging technique that the spectral imaging technology that quickly grows at present generates,
It is the combination of spectral analysis technique and micro-imaging technique, can provide two-dimensional space information and one-dimensional spectrum letter simultaneously
Breath, coordinates certain Data Analysis Services algorithm, can realize qualitative, quantitative, positioning the comprehensive analysis of material composition composition.
Existing microspectrum imaging technique can be divided into two major classes from imaging mode：
One kind is the microspectrum imaging technique based on time dimension sweep type, and the main light splitting means of such technology include
Three kinds of acousto-optic tunable filter (AOTF), liquid crystal tunable optical filter (LCTF) and rotating filtering piece.Publication No.
A kind of microspectrum imaging device based on AOTF of the disclosure of the invention of CN102226756A；Publication No. CN107064019A,
A kind of microspectrum imaging device based on liquid crystal tunable optical filter of the disclosure of the invention of CN103323410.Based on AOTF points
The method of light has many advantages, such as that simple, the tunable wide waveband of light splitting, tuned speed are fast, without motion mechanism, but the device is in band
There are aerial image shifting when broad tuning, image blur is caused, this is very harmful for micro imaging system.Based on LCTF
The advantages of method of light splitting equally has light splitting simple, movement-less part, but the device wavelength band is generally relatively narrow, it is serious to limit
Made the detectable spectral coverage of system, and haveed the shortcomings that spectral transmittance is low, so seriously affected the signal-to-noise ratio of system with
The clarity of imaging.A kind of tunable band-pass optical filter device of the disclosure of the invention of Publication No. CN102589694 it is multispectral aobvious
Micro- image acquiring device, band-limited workable for the light-splitting method, can only in the optical filter quantity installed on optical filter wheel
The transformation for realizing limited several wave bands can not achieve the spectral coverage transformation within the scope of broadband continuous wavelength, therefore can only be directed to spy
Fixed several tissues are diagnosed, and cannot be diagnosed to a variety of pathological tissues.Microspectrum based on time dimension sweep type at
Scanning as technology due to needing time dimension, could obtain the spectral information of target different spectral coverage, therefore, it is difficult to realize videl stage
The quick obtaining of target three-dimensional information (the one-dimensional spectrum of two-dimensional space information).
In addition one kind is the microspectrum imaging technique of space dimension sweep type：The main light splitting means of such imaging technique have
Prism, grating or prism and grating combination are divided.The invention of existing Publication No. CN1563947A and CN103411934A is equal
Light splitting (product price is relatively high) is realized using foreign standardization business light splitting product I mSpector, and by existing
Increase precise electric control on microscope and pushes away the bidimensional swept and finally obtain observed target to realize the acquisition of other one-dimensional space information
Spatial information and one-dimensional spectral information.The advantages that this method has spectral region wide, and spectral resolution is high, and system transmitance is high,
But need to increase on existing microscope high-precision sweep mechanism to realize the acquisition of other one-dimensional space information, to existing aobvious
Micro-system change is larger, and integrated level is not high, and system structure is more complicated.Microspectrum imaging technique based on space dimension sweep type
Due to needing the scanning of one space dimension of progress, the other one-dimensional space information that target could be obtained (otherwise to can only obtain one-dimensional sky
Between information and one-dimensional optical information), therefore be also difficult to realize videl stage target three-dimensional information (the one-dimensional spectrum of two-dimensional space information)
Quick obtaining.
Invention content
It takes time present invention aim to address existing microspectrum imaging method and ties up scanning or space dimension scanning, lead to difficulty
To fast implement acquisition and the imaging system complex structure and other problems of target three-dimensional information, a kind of micro- bloom of snapshot-type is provided
Spectrum imaging system and imaging method, which does not need time dimension when obtaining target three-dimensional information or space dimension is swept
It retouches, once photo taking can be obtained the two-dimensional space information of target and one-dimensional spectral information, imaging time high resolution, can be used for pair
Quickly variation or moving target three-dimensional information real-time acquisition, and by standard interface can directly with existing standardization
Microscope is docked, very easy to use, and moving component is not present in system, and reliability is high.
The technical scheme is that：
A kind of snapshot-type high microspectrum imaging system, including lighting system, objective table, microcobjective, beam splitter, meter
Calculation machine, acquisition system and compensation system；The lighting system is for illuminating the target to be seen on objective table；Described point
Optical element by the light beam of microcobjective by splitting ratio to being divided；The acquisition system includes being successively set on beam splitter
The first imaging lens, microlens array, collimating mirror, dispersion element, the second imaging lens on transmitted light path and the first detector；It is described
Compensation system includes the third imaging lens and the second detector being successively set on beam splitter reflected light path；First detection
Device and the second detector are connect with computer respectively.
Further, the microlens array is located at the image planes position of the first imaging lens.
Further, the image planes of the microlens array are located at the object space position of focal plane of collimating mirror.
Further, the beam splitter is beam-splitter or Amici prism.
Meanwhile the present invention also provides a kind of micro- high spectrum imaging method of snapshot-type, including the following steps：
1) object observing is treated to be illuminated；
2) microcobjective is treated object observing and is amplified, object observing detailed information；
3) it will be divided by splitting ratio by the light beam of microcobjective；
4) wherein light converges at microlens array after being imaged all the way, and several subgraph arrays are divided by microlens array,
The dispersion pattern matrix for being divided subgraph is obtained after the collimated mirror of each subgraph, dispersion element on the detector, is observed
The one-dimensional hyperspectral information and bidimensional low spatial resolution image information of target；
5) in addition reflected light direct imaging all the way, obtains the high spatial resolution images information of target to be observed；
6) the bidimensional low spatial resolution figure for obtaining the high spatial resolution images information that step 5) obtains to step 4)
As carrying out Space Reconstruction, bidimensional high-resolution image information is obtained, the bidimensional high spatial resolution figure letter of target is finally obtained
Breath and one-dimensional hyperspectral information.
Further, the dispersion element is grating, prism or grating and prism arrangement.
Further, grating is reflecting grating or transmission grating.
Further, prism is monolithic prism or combined prism.
Compared with prior art, the present invention having the following technical effects：
1. motion is not present in the invention, system structure is simple, and reliability is high, and need not be to existing microscope
Any change design is done, can be realized by standard interface and docked with existing micro-imaging, realization treats object observing three-dimensional
The quick obtaining of information.
2. the invention obtains, target three-dimensional information does not need time dimension or space dimension scanning, once photo taking can be obtained target
Two-dimensional space information and one-dimensional spectral information, imaging time high resolution is, it can be achieved that the real-time of variation targets three-dimensional information
Quick obtaining.
Description of the drawings
Fig. 1 is snapshot-type high microspectrum imaging system structure chart of the present invention.
Reference numeral：1- lighting systems；2- objective tables；3- microcobjectives；4- beam splitters；The first imaging lens of 5-；6- is micro-
Lens array；7- microlens array image planes position；8- collimating mirrors；9- dispersion elements；The second imaging lens of 10-；11- first is detected
Device；12- third imaging lens；The second detectors of 13-；14- computers.
Specific implementation mode
Snapshot-type high microspectrum imaging system as shown in Figure 1, including lighting system 1, objective table 2, microcobjective 3,
Beam splitter 4, computer 14, acquisition system and compensation system；Lighting system 1 be used for the target to be seen on objective table 2 into
Row Uniform Illumination；Beam splitter 4 is divided the light beam by microcobjective 3 by certain splitting ratio；Acquisition system include according to
Secondary the first imaging lens 5 being arranged on 4 transmitted light path of beam splitter, microlens array 6, collimating mirror 8, dispersion element 9, the second one-tenth
As mirror 10 and the first detector 11, microlens array 6 is located at the image planes position of the first imaging lens 5, microlens array image planes position 7
On the object space focal plane of collimating mirror 8；Compensation system includes the third imaging lens being successively set on 4 reflected light path of beam splitter
12 and second detector 13；First detector 11 and the second detector 13 are connect with computer 14 respectively, and computer 14 is to obtaining
Information handled.
Beam splitter 4 can be beam-splitter or Amici prism, and dispersion element 9 can be that grating can also be prism or the two
Combination, grating can be that reflecting grating can also be transmission grating, and prism can be that monolithic prism can also be several pieces of prisms
Combination.Lighting system 1 includes light source and lamp optical system, realizes the Uniform Illumination to being observed target.
Meanwhile the present invention also provides a kind of micro- high spectrum imaging method of snapshot-type, including the following steps：
1) it treats object observing and carries out Uniform Illumination；
2) microcobjective is treated object observing and is amplified, with the detailed information of object observing；
3) by by the light beam of microcobjective by certain splitting ratio (such as：Reflection ratio is 0.3, and transmission proportion is
0.7) it is divided；
4) transmitted light converges at microlens array by the first imaging lens, and several subgraph battle arrays are divided by microlens array
Row, each subgraph is collimated, dispersion, is imaged on the first detector different location, obtains the dispersion image battle array for being divided subgraph
Row obtain the one-dimensional hyperspectral information and bidimensional low-resolution spatial information of target to be observed；
5) in addition reflected light direct imaging all the way, obtains the high spatial resolution images information of target to be observed；
6) the bidimensional low spatial resolution that the high spatial resolution images information for utilizing step 5) to obtain obtains step 4)
Image carries out Space Reconstruction and finally obtains the bidimensional high spatial resolution figure of target to obtain bidimensional high-resolution image information
Shape information and one-dimensional hyperspectral information.
Step 4 of the present invention can obtain target to be seen different spectral coverage multiple image, by the figure of these different spectral coverages
As carrying out degradation analysis first, the full resolution pricture then obtained using in step 5 leads to as the benchmark of reconstruct high-definition picture
Cross corresponding super-resolution image reconstruction method.For example, utilizing sparse coding method, deep learning method, bayesian algorithm etc.
Reconstruct the two dimensional image information of a panel height spatial resolution.
Present system operation principle：By lighting system 1, target to be seen on objective table 2 is subjected to Uniform Illumination, is shown
Speck mirror 3 forms amplification system with the first imaging lens 5 or third imaging lens 12 and is convenient for object observing thin target to be seen amplification
Information is saved, beam splitter 4 for will be divided by certain splitting ratio by the light beam of microcobjective 3 (also may be used by beam splitter 4
To be the Amici prism or beam-splitter of energy light splitting effect), it is divided into two-way.
Microlens array 6 wherein is converged at by the first imaging lens 5 all the way, microlens array 6 is located at the first imaging lens 5
On imaging surface, the part that each lenslet treats object observing is imaged, and target to be seen is divided by microlens array 6
Several subgraph arrays, these subgraphs realize the collimation of light beam by collimating mirror 8 again, the after each subgraph dispersion element 9
It obtains being divided the dispersion pattern matrix of subgraph and the two-dimensional space information of subgraph on one detector 11.
In addition the second detector 13, the height for obtaining target to be observed are direct imaging at by third imaging lens 12 all the way
Spatial resolution image information, the bidimensional low spatial resolution image information for compensating the first detector 11.Second detector
The high spatial resolution images information obtained on 13 carries out image co-registration with the image information obtained on the first detector 11, with
The two-dimensional space information and high spectral resolution spectral information of high spatial resolution are obtained, control computer 14 is for controlling two
Data acquisition, data storage and the Image data reconstruction of detector (being planar array detector).
Claims (10)
1. a kind of snapshot-type high microspectrum imaging system, it is characterised in that：Including lighting system (1), objective table (2), micro-
Object lens (3), beam splitter (4), computer (14), acquisition system and compensation system；
The lighting system (1) is for illuminating the target to be seen on objective table (2)；
The beam splitter (4) is divided the light beam by microcobjective (3) by splitting ratio；
The acquisition system includes the first imaging lens (5), the microlens array being successively set on beam splitter (4) transmitted light path
(6), collimating mirror (8), dispersion element (9), the second imaging lens (10) and the first detector (11)；
The compensation system includes the third imaging lens (12) being successively set on beam splitter (4) reflected light path and the second detection
Device (13)；
First detector (11) and the second detector (13) are connect with computer (14) respectively.
2. snapshot-type high microspectrum imaging system according to claim 1, it is characterised in that：The microlens array
(6) it is located at the image planes position of the first imaging lens (5).
3. snapshot-type high microspectrum imaging system according to claim 2, it is characterised in that：The microlens array
(6) image planes are located at the object space position of focal plane of collimating mirror (8).
4. snapshot-type high microspectrum imaging system according to claim 1 or 2 or 3, it is characterised in that：The light splitting member
Part (4) is beam-splitter or Amici prism.
5. snapshot-type high microspectrum imaging system according to claim 4, it is characterised in that：The dispersion element (9)
For the combination of grating, prism or grating and prism.
6. snapshot-type high microspectrum imaging system according to claim 5, it is characterised in that：Grating be reflecting grating or
Transmission grating.
7. snapshot-type high microspectrum imaging system according to claim 5, it is characterised in that：Prism be monolithic prism or
Combined prism.
8. a kind of micro- high spectrum imaging method of snapshot-type, which is characterized in that include the following steps：
1) object observing is treated to be illuminated；
2) microcobjective is treated object observing and is amplified, object observing detailed information；
3) it will be divided by splitting ratio by the light beam of microcobjective；
4) wherein light converges at microlens array after being imaged all the way, and several subgraph arrays, each son are divided by microlens array
The dispersion pattern matrix for being divided subgraph is obtained after the collimated mirror of image, dispersion element on the detector, obtains observed object
One-dimensional hyperspectral information and bidimensional low spatial resolution image information；
5) in addition reflected light direct imaging all the way, obtains the high spatial resolution images information of target to be observed；
6) the bidimensional low spatial resolution image for obtaining the high spatial resolution images information that step 5) obtains to step 4) into
Row Space Reconstruction obtains bidimensional high-resolution image information, finally obtain target bidimensional high spatial resolution graphical information and
One-dimensional hyperspectral information.
9. the micro- high spectrum imaging method of snapshot-type according to claim 8, it is characterised in that：Dispersion member in step 4)
Part is the combination of grating, prism or grating and prism.
10. the micro- high spectrum imaging method of snapshot-type according to claim 9, it is characterised in that：Light described in step 4)
Grid are reflecting grating or transmission grating, and the prism is monolithic prism or combined prism.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109270678A (en) * | 2018-10-31 | 2019-01-25 | 许之敏 | A kind of novel high-resolution light field microscope structure |
| CN109342328A (en) * | 2018-10-11 | 2019-02-15 | 中国科学院西安光学精密机械研究所 | A kind of built-in scan type high microspectrum imaging system and imaging method |
| CN109490223A (en) * | 2018-11-20 | 2019-03-19 | 东北大学 | A kind of target acquisition identifying system and method based on programmable high light spectrum image-forming |
| CN111505819A (en) * | 2020-05-21 | 2020-08-07 | 杨星瑶 | Microscopy instrument and method for repeated positioning and measurement by using digital sequence image |
| CN112097905A (en) * | 2020-08-17 | 2020-12-18 | 杭州电子科技大学 | Spectral microscopic imaging device |
| CN112098337A (en) * | 2020-08-31 | 2020-12-18 | 清华大学深圳国际研究生院 | High-resolution spectral image rapid acquisition device and method |
| CN112197863A (en) * | 2020-12-04 | 2021-01-08 | 武汉精测电子集团股份有限公司 | Lattice spectrum measuring device, area array chromaticity measuring device and method |
| CN113514944A (en) * | 2021-03-31 | 2021-10-19 | 杭州电子科技大学 | Quick spectral microscopic imaging device |
| CN113790798A (en) * | 2021-09-03 | 2021-12-14 | 中国科学院西安光学精密机械研究所 | Seamless spectral imaging device, system and method for dynamic point target tracking measurement |
| CN114112948A (en) * | 2021-10-22 | 2022-03-01 | 光子集成(温州)创新研究院 | Multispectral microscopic imaging system and control method thereof |
| CN115950837A (en) * | 2023-03-10 | 2023-04-11 | 湖南师范大学 | Snapshot type spectral imaging method, system and medium based on plug-and-play prior |
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| CN109342328A (en) * | 2018-10-11 | 2019-02-15 | 中国科学院西安光学精密机械研究所 | A kind of built-in scan type high microspectrum imaging system and imaging method |
| CN109270678A (en) * | 2018-10-31 | 2019-01-25 | 许之敏 | A kind of novel high-resolution light field microscope structure |
| CN109490223A (en) * | 2018-11-20 | 2019-03-19 | 东北大学 | A kind of target acquisition identifying system and method based on programmable high light spectrum image-forming |
| CN111505819A (en) * | 2020-05-21 | 2020-08-07 | 杨星瑶 | Microscopy instrument and method for repeated positioning and measurement by using digital sequence image |
| CN112097905A (en) * | 2020-08-17 | 2020-12-18 | 杭州电子科技大学 | Spectral microscopic imaging device |
| CN112097905B (en) * | 2020-08-17 | 2022-12-20 | 杭州电子科技大学 | Spectral microscopic imaging device |
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| WO2022116412A1 (en) * | 2020-12-04 | 2022-06-09 | 武汉精测电子集团股份有限公司 | Dot matrix spectrum measurement apparatus and method, and area array chrominance measurement apparatus and method |
| CN112197863A (en) * | 2020-12-04 | 2021-01-08 | 武汉精测电子集团股份有限公司 | Lattice spectrum measuring device, area array chromaticity measuring device and method |
| CN113514944A (en) * | 2021-03-31 | 2021-10-19 | 杭州电子科技大学 | Quick spectral microscopic imaging device |
| CN113514944B (en) * | 2021-03-31 | 2023-02-17 | 杭州电子科技大学 | Quick spectral microscopic imaging device |
| CN113790798A (en) * | 2021-09-03 | 2021-12-14 | 中国科学院西安光学精密机械研究所 | Seamless spectral imaging device, system and method for dynamic point target tracking measurement |
| CN113790798B (en) * | 2021-09-03 | 2022-07-19 | 中国科学院西安光学精密机械研究所 | Seamless spectral imaging device, system and method for dynamic point target tracking measurement |
| CN114112948A (en) * | 2021-10-22 | 2022-03-01 | 光子集成(温州)创新研究院 | Multispectral microscopic imaging system and control method thereof |
| CN115950837A (en) * | 2023-03-10 | 2023-04-11 | 湖南师范大学 | Snapshot type spectral imaging method, system and medium based on plug-and-play prior |
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