CN205228640U - Multi -functional micro - spectral imaging system of scanning formula - Google Patents
Multi -functional micro - spectral imaging system of scanning formula Download PDFInfo
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- CN205228640U CN205228640U CN201520999237.5U CN201520999237U CN205228640U CN 205228640 U CN205228640 U CN 205228640U CN 201520999237 U CN201520999237 U CN 201520999237U CN 205228640 U CN205228640 U CN 205228640U
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- beam splitter
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Abstract
The utility model provides a multi -functional micro - spectral imaging system of scanning formula, includes pulsed laser light source or continuous spectrum light source, computer, three -dimensional electronic accurate translation objective table, photoelectric detector, heat mirror - beam splitter auto -change over device, light scanning device, objective, spectrum appearance, beam splitter, mechanical optical switch, speculum, data acquisition system. The utility model discloses a micro - spectral imaging system passes through computer control light scanning device or three -dimensional electronic accurate translation objective table point by point scanning, in the spectrum appearance camera synchronous sampling sample dorsad or preceding to fluorescence (Raman light), the spectral information of scattered light, data acquisition system can take notes the spectral information of spectrum appearance at every scanning element, on accomplishing the sample plane behind regional data acquisition that awaits measuring, the microscopy images of the fluorescence (Raman light) of sample micro - or scattered light is generated by the computer, and generate the microscopy images and the three -dimensional micro - spectral image of the different spectrum wave bands of sample simultaneously.
Description
Technical field
The utility model relates to micro-imaging field, is specifically related to a kind of scan-type Multifunctional display low-light spectrum imaging system.
Background technology
Micro-imaging technique is long-standing, and it is the most often applied to the sample that observation naked eyes cannot be observed, and application comprises the fields such as biomedicine, chemistry, physics, metallurgy, measurement, has unique position in the development in a lot of field.Meanwhile, micro-imaging technique is also in constantly development is improved, and the field formula microscope from, to confocal microscope afterwards, it has had important breakthrough in enlargement factor, noise resistance etc.But, no matter be traditional field formula microscope, or confocal microscope, be all utilize the light intensity of sample scattering light to imaging of samples, traditional micro-imaging can only obtain the structural images of sample.Even if fluorescence imaging also just obtains the micro-image of a certain wave band fluorescent intensity of sample, the complete spectrum characteristic information of sample can not be obtained.And traditional micro imaging system can not realize multiple imaging function simultaneously.
Utility model content
For the deficiency of traditional microscopic system, in order to obtain the more characteristic information of sample, the utility model proposes a kind of scan-type Multifunctional display low-light spectrum imaging system combined with microtechnic by spectral imaging technology, by recording fluorescence (Raman light) spectrum that excites in the sample to which or scattered light spectrum, realize that fluorescence (Raman light) to sample is micro-, fluorescence (Raman light) spectrum is micro-or scattered light is micro-and the imaging of scattered light microspectrum.
The technical solution of the utility model is as follows:
The utility model provides a kind of scan-type Multifunctional display low-light spectrum imaging system, includes computing machine, three-D electric accurate translation stage, the pulsed laser light source of collimation, photodetector, the continuous spectrum light source of collimation, heat mirror-beam splitter switching device shifter, light scanning apparatus, object lens, spectrometer, beam splitter, mechanical optical switch, catoptron, data acquisition system (DAS).
Computing machine connects three-D electric accurate translation stage, pulsed laser light source, photodetector, light scanning apparatus and data acquisition system (DAS) simultaneously, and data acquisition system (DAS) connects the camera of spectrometer.The pulse laser that pulsed laser light source sends is divided into two bundles after beam splitter, a branch ofly by photoelectric detector, electric signal passed to computing machine; Another bundle is divided into two bundles again by beam splitter, a branch of sample on mechanical optical switch, heat mirror-beam splitter switching device shifter, light scanning apparatus, the accurate translation stage of object lens arrival three-D electric; The sample of another bundle on mechanical optical switch, catoptron, the accurate translation stage of object lens arrival three-D electric.The light that continuous spectrum light source sends reflects through catoptron, is divided into two bundles by through beam splitter, a branch of sample on mechanical optical switch, heat mirror-beam splitter switching device shifter, light scanning apparatus, the accurate translation stage of object lens arrival three-D electric.The sample of another bundle on mechanical optical switch, catoptron, the accurate translation stage of object lens arrival three-D electric.
Advantageous Effects of the present utility model is:
The utility model is when unbalanced pulse LASER Light Source, trigger pip is provided to data acquisition system (DAS) by photodetector, open in two mechanical optical switches, by the accurate translation stage scanning of light scanning apparatus or three-D electric, sample can be obtained dorsad or the fluorescence of forward direction (Raman light) is micro-and fluorescence (Raman light) microspectrum image.
The utility model is when opening continuous spectrum light source, by opening in two mechanical optical switches, by the accurate translation stage scanning of light scanning apparatus or three-D electric, sample can be obtained dorsad or the spectrum picture that the scattered light of forward direction is micro-and scattered light is micro-.
Accompanying drawing explanation
Fig. 1 is system construction drawing of the present utility model.
Fig. 2 be gather dorsad fluorescence (Raman light) time, the structural drawing of system.
Fig. 3 is when gathering forward direction fluorescence (Raman light), the structural drawing of system.
Fig. 4 is when gathering backscattering light, the structural drawing of system.
Fig. 5 is when gathering forward scattering light, the structural drawing of system.
[reference numeral explanation] 1. computing machine; 2. the accurate translation stage of three-D electric; 3. pulsed laser light source; 4. photodetector; 5. spectrometer; 6. the first beam splitter; 7. the second beam splitter; 8. heat mirror-beam splitter switching device shifter; 9. light scanning apparatus; 10. the first object lens; 11. first mechanical optical switches; 12. first catoptrons; 13. second catoptrons; 14. second object lens; 15. continuous spectrum light sources; 16. the 3rd catoptrons; 17. second mechanical optical switches; 18. data acquisition system (DAS)s.
Embodiment
Below in conjunction with accompanying drawing, the utility model will be further described.
As shown in Figure 1, scan-type Multifunctional display low-light spectrum imaging system of the present utility model comprises computing machine 1, three-D electric accurate translation stage 2, pulsed laser light source 3, photodetector 4, spectrometer 5, first beam splitter 6, second beam splitter 7, heat mirror-beam splitter switching device shifter 8, light scanning apparatus 9, first object lens 10, second object lens 14, first mechanical optical switch 11, second mechanical optical switch 17, first catoptron 12, second catoptron 13, the 3rd catoptron 16, continuous spectrum light source 15, data acquisition system (DAS) 18.
As shown in Figure 1, computing machine 1 connects three-D electric accurate translation stage 2, pulsed light laser light source 3, photodetector 4, light scanning apparatus 9 and data acquisition system (DAS) 18 simultaneously, and data acquisition system (DAS) 18 connects the camera of spectrometer 5.The pulse laser that pulsed laser light source 3 sends is divided into two bundles after the first beam splitter 6, is a branch ofly received by photodetector 4 and electric signal is passed to computing machine 1; Another bundle is divided into two bundles again by point second beam splitter 7, a branch of sample arrived through the second mechanical optical switch 17, heat mirror-beam splitter switching device shifter 8, light scanning apparatus 9, first object lens 10 on the accurate translation stage 2 of three-D electric; Another bundle arrives the sample on the accurate translation stage 2 of three-D electric through the first mechanical optical switch 11, first catoptron 12, second catoptron 13, second object lens 14.The light that continuous spectrum light source 15 sends reflects through the 3rd catoptron 16, two bundles are divided into, a branch of sample arrived through the second mechanical optical switch 17, heat mirror-beam splitter switching device shifter 8, light scanning apparatus 9, first object lens 10 on the accurate translation stage 2 of three-D electric via point second beam splitter 7.Another bundle arrives the sample on the accurate translation stage 2 of three-D electric through the first mechanical optical switch 11, first catoptron 12, second catoptron 13, second object lens 14.
Scan-type Multifunctional display low-light spectrum imaging system of the present utility model can realize two kinds of functions, and often kind of function has two kinds of mode of operations respectively.
1, micro-, fluorescence (Raman light) the microspectrum imaging function of fluorescence (Raman light)
In the system construction drawing shown in Fig. 1, open pulsed laser light source 3, open photodetector 4, close the first mechanical optical switch 11, open the second mechanical optical switch 17, heat mirror-beam splitter switching device shifter 8 is arranged on heat mirror position.To be scanned by light scanning apparatus or the scanning of electric precision translation stage realizes that fluorescence (Raman light) is micro-, fluorescence (Raman light) microspectrum imaging function, system is reduced to as shown in Figure 2, now, the fluorescence of fluorescence (Raman light) (Raman light) is micro-, fluorescence (Raman light) microspectrum imaging pattern gathering dorsad for system works.
In the system construction drawing shown in Fig. 1, open pulsed laser light source 3, open photodetector 4, open the first mechanical optical switch 11, close machine second mechanical optical switch 17, heat mirror-beam splitter switching device shifter 8 is arranged on heat mirror position.Realize that fluorescence (Raman light) is micro-, fluorescence (Raman light) microspectrum imaging function by electric precision translation stage 2 scanning, system simplifies as shown in Figure 3, now, micro-, fluorescence (Raman light) the microspectrum imaging pattern of the system works fluorescence (Raman light) that gathers fluorescence (Raman light) at forward direction.
2, scattered light micro-imaging, scattered light microspectrum imaging function
In the system construction drawing shown in Fig. 1, open continuous spectrum light source 15, close the first mechanical optical switch 11, open the second mechanical optical switch 17, heat mirror-beam splitter switching device shifter 8 is arranged on beam splitter position.Scanned by light scanning apparatus or electric precision translation stage scanning realize scattered light micro-imaging, scattered light microspectrum imaging function, system is reduced to as shown in Figure 3, now, system works, scattered light microspectrum imaging pattern micro-at the scattered light of collection of scattered light dorsad.
In the system construction drawing shown in Fig. 1, open continuous spectrum light source 15, open the first mechanical optical switch 11, close the second mechanical optical switch 17, heat mirror-beam splitter switching device shifter 8 is arranged on beam splitter position.Realize that scattered light is micro-, scattered light microspectrum imaging function by electric precision translation stage 2 scanning, system simplifies as shown in Figure 4, and now, system works is micro-at the scattered light of forward direction collection of scattered light, scattered light microspectrum imaging pattern.
Above-described is only preferred implementation of the present utility model, and the utility model is not limited to above embodiment.Be appreciated that the oher improvements and changes that those skilled in the art directly derive or associate under the prerequisite not departing from basic conception of the present utility model, all should think and be included within protection domain of the present utility model.
Claims (1)
1. a scan-type Multifunctional display low-light spectrum imaging system, it is characterized in that: comprise computing machine (1), the accurate translation stage (2) of three-D electric, pulsed laser light source (3), photodetector (4), continuous spectrum light source (15), heat mirror-beam splitter switching device shifter (8), light scanning apparatus (9), first object lens (10), second object lens (14), spectrometer (5), first beam splitter (6), second beam splitter (7), first mechanical optical switch (11), second mechanical optical switch (17), first catoptron (12), second catoptron (13), 3rd catoptron (16), data acquisition system (DAS) (18), computing machine (1) connects the accurate translation stage (2) of three-D electric simultaneously, pulsed laser light source (3), photodetector (4), light scanning apparatus (9) and data acquisition system (DAS) (18), data acquisition system (DAS) (18) connects the camera of spectrometer (5), the pulse laser that pulsed laser light source (3) sends is divided into two bundles after the first beam splitter (6), a branch of by photodetector (4) receive and electric signal is passed to computing machine (1), another bundle is divided into two bundles again by the second beam splitter (7), a branch of through the second mechanical optical switch (17), heat mirror-beam splitter switching device shifter (8), light scanning apparatus (9), first object lens (10) arrive the sample on the accurate translation stage (2) of three-D electric, the sample of another bundle on the first mechanical optical switch (11), the first catoptron (12), the second catoptron (13), the accurate translation stage (2) of the second object lens (14) arrival three-D electric, the light that continuous spectrum light source (15) sends reflects through the 3rd catoptron (16), two bundles are divided into via the second beam splitter (7), a branch of through the second mechanical optical switch (17), heat mirror-beam splitter switching device shifter (8), light scanning apparatus (9), first object lens (10) arrive the sample on the accurate translation stage (2) of three-D electric, another bundle is through the first mechanical optical switch (11), first catoptron (12), second catoptron (13), second object lens (14) arrive the sample on the accurate translation stage (2) of three-D electric.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520999237.5U CN205228640U (en) | 2015-12-04 | 2015-12-04 | Multi -functional micro - spectral imaging system of scanning formula |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520999237.5U CN205228640U (en) | 2015-12-04 | 2015-12-04 | Multi -functional micro - spectral imaging system of scanning formula |
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| CN205228640U true CN205228640U (en) | 2016-05-11 |
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| CN201520999237.5U Withdrawn - After Issue CN205228640U (en) | 2015-12-04 | 2015-12-04 | Multi -functional micro - spectral imaging system of scanning formula |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424189A (en) * | 2015-12-04 | 2016-03-23 | 江南大学 | Scanning type multifunctional microscopic-spectral imaging method |
| CN111220615A (en) * | 2019-10-29 | 2020-06-02 | 怀光智能科技（武汉）有限公司 | Inclined three-dimensional scanning microscopic imaging system and method |
-
2015
- 2015-12-04 CN CN201520999237.5U patent/CN205228640U/en not_active Withdrawn - After Issue
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424189A (en) * | 2015-12-04 | 2016-03-23 | 江南大学 | Scanning type multifunctional microscopic-spectral imaging method |
| CN105424189B (en) * | 2015-12-04 | 2017-11-07 | 江南大学 | A kind of multi-functional microspectrum imaging system of scan-type |
| CN111220615A (en) * | 2019-10-29 | 2020-06-02 | 怀光智能科技（武汉）有限公司 | Inclined three-dimensional scanning microscopic imaging system and method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20160511 Effective date of abandoning: 20171107 |
|
| AV01 | Patent right actively abandoned |