GB2574197B - Double stranded polynucleotide synthesis method and system. - Google Patents

Double stranded polynucleotide synthesis method and system. Download PDF

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Publication number
GB2574197B
GB2574197B GB1808474.9A GB201808474A GB2574197B GB 2574197 B GB2574197 B GB 2574197B GB 201808474 A GB201808474 A GB 201808474A GB 2574197 B GB2574197 B GB 2574197B
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Prior art keywords
double stranded
synthesis method
stranded polynucleotide
polynucleotide synthesis
double
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GB201808474D0 (en
GB2574197A (en
Inventor
Milton John
Nayyar Sobia
Riedl Jan
Ogaki Ryosuke
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Oxford Nanopore Technologies PLC
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Oxford Nanopore Technologies PLC
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Priority to GB1808474.9A priority Critical patent/GB2574197B/en
Publication of GB201808474D0 publication Critical patent/GB201808474D0/en
Priority to CN201980038640.2A priority patent/CN112292456B/zh
Priority to AU2019274939A priority patent/AU2019274939B2/en
Priority to US17/057,140 priority patent/US12448635B2/en
Priority to PCT/GB2019/051423 priority patent/WO2019224544A1/en
Priority to JP2020564936A priority patent/JP7393351B2/ja
Priority to CA3099103A priority patent/CA3099103A1/en
Priority to EP19730428.0A priority patent/EP3797170B1/en
Publication of GB2574197A publication Critical patent/GB2574197A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/319—Exonuclease
    • C—CHEMISTRY; METALLURGY
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/531—Glycosylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components

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  • General Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
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  • Biomedical Technology (AREA)
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  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
GB1808474.9A 2018-05-23 2018-05-23 Double stranded polynucleotide synthesis method and system. Active GB2574197B (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
GB1808474.9A GB2574197B (en) 2018-05-23 2018-05-23 Double stranded polynucleotide synthesis method and system.
PCT/GB2019/051423 WO2019224544A1 (en) 2018-05-23 2019-05-23 Polynucleotide synthesis method, system and kit
AU2019274939A AU2019274939B2 (en) 2018-05-23 2019-05-23 Polynucleotide synthesis method, system and kit
US17/057,140 US12448635B2 (en) 2018-05-23 2019-05-23 Polynucleotide synthesis method, system and kit
CN201980038640.2A CN112292456B (zh) 2018-05-23 2019-05-23 多核苷酸合成方法、系统和试剂盒
JP2020564936A JP7393351B2 (ja) 2018-05-23 2019-05-23 ポリヌクレオチドの合成法、システム、およびキット
CA3099103A CA3099103A1 (en) 2018-05-23 2019-05-23 Polynucleotide synthesis method, system and kit
EP19730428.0A EP3797170B1 (en) 2018-05-23 2019-05-23 Polynucleotide synthesis method, system and kit

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GB1808474.9A GB2574197B (en) 2018-05-23 2018-05-23 Double stranded polynucleotide synthesis method and system.

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GB201808474D0 GB201808474D0 (en) 2018-07-11
GB2574197A GB2574197A (en) 2019-12-04
GB2574197B true GB2574197B (en) 2022-01-05

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EP (1) EP3797170B1 (https=)
JP (1) JP7393351B2 (https=)
CN (1) CN112292456B (https=)
AU (1) AU2019274939B2 (https=)
CA (1) CA3099103A1 (https=)
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EP3797170A1 (en) 2021-03-31
WO2019224544A1 (en) 2019-11-28
US12448635B2 (en) 2025-10-21
AU2019274939B2 (en) 2025-05-22
US20210198710A1 (en) 2021-07-01
AU2019274939A1 (en) 2020-11-12
EP3797170B1 (en) 2023-07-19
JP7393351B2 (ja) 2023-12-06
GB201808474D0 (en) 2018-07-11
CN112292456A (zh) 2021-01-29
CN112292456B (zh) 2025-06-13
CA3099103A1 (en) 2019-11-28
GB2574197A (en) 2019-12-04
JP2021524246A (ja) 2021-09-13

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