AU2019274939B2 - Polynucleotide synthesis method, system and kit - Google Patents

Polynucleotide synthesis method, system and kit Download PDF

Info

Publication number
AU2019274939B2
AU2019274939B2 AU2019274939A AU2019274939A AU2019274939B2 AU 2019274939 B2 AU2019274939 B2 AU 2019274939B2 AU 2019274939 A AU2019274939 A AU 2019274939A AU 2019274939 A AU2019274939 A AU 2019274939A AU 2019274939 B2 AU2019274939 B2 AU 2019274939B2
Authority
AU
Australia
Prior art keywords
strand
nucleotide
ligation
polynucleotide
synthesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2019274939A
Other languages
English (en)
Other versions
AU2019274939A1 (en
Inventor
John Milton
Sobia NAYYAR
Ryosuke OGAKI
Jan RIEDL
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford Nanopore Technologies PLC
Original Assignee
Oxford Nanopore Technologies PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Nanopore Technologies PLC filed Critical Oxford Nanopore Technologies PLC
Publication of AU2019274939A1 publication Critical patent/AU2019274939A1/en
Assigned to OXFORD NANOPORE TECHNOLOGIES PLC reassignment OXFORD NANOPORE TECHNOLOGIES PLC Amend patent request/document other than specification (104) Assignors: OXFORD NANOPORE TECHNOLOGIES LIMITED
Application granted granted Critical
Publication of AU2019274939B2 publication Critical patent/AU2019274939B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/319—Exonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/531—Glycosylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2019274939A 2018-05-23 2019-05-23 Polynucleotide synthesis method, system and kit Active AU2019274939B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1808474.9 2018-05-23
GB1808474.9A GB2574197B (en) 2018-05-23 2018-05-23 Double stranded polynucleotide synthesis method and system.
PCT/GB2019/051423 WO2019224544A1 (en) 2018-05-23 2019-05-23 Polynucleotide synthesis method, system and kit

Publications (2)

Publication Number Publication Date
AU2019274939A1 AU2019274939A1 (en) 2020-11-12
AU2019274939B2 true AU2019274939B2 (en) 2025-05-22

Family

ID=62812598

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2019274939A Active AU2019274939B2 (en) 2018-05-23 2019-05-23 Polynucleotide synthesis method, system and kit

Country Status (8)

Country Link
US (1) US12448635B2 (https=)
EP (1) EP3797170B1 (https=)
JP (1) JP7393351B2 (https=)
CN (1) CN112292456B (https=)
AU (1) AU2019274939B2 (https=)
CA (1) CA3099103A1 (https=)
GB (1) GB2574197B (https=)
WO (1) WO2019224544A1 (https=)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2563105B (en) 2016-11-16 2022-10-19 Catalog Tech Inc Nucleic acid-based data storage
GB2559117B (en) 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
AU2019236289B2 (en) 2018-03-16 2024-12-05 Catalog Technologies, Inc. Chemical methods for nucleic acid-based data storage
AU2019270160B2 (en) 2018-05-16 2024-09-19 Catalog Technologies, Inc. Printer-finisher system for data storage in DNA
GB2574197B (en) 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB201811813D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811810D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
AU2019315604B2 (en) 2018-08-03 2025-04-17 Catolog Technologies, Inc Systems and methods for storing and reading nucleic acid-based data with error protection
KR20220104057A (ko) * 2019-12-30 2022-07-25 위안디안 바이오랩스 컴퍼니 리미티드 효소를 이용한 핵산 서열 제조 장치 및 방법
US20220356500A1 (en) * 2019-12-30 2022-11-10 Yuandian Biolabs Co., Ltd. Method for preparing nucleic acid sequences using enzyme
US20230117083A1 (en) * 2020-01-22 2023-04-20 Nuclera Nucleics Ltd. Methods of nucleic acid synthesis
GB202013102D0 (en) * 2020-08-21 2020-10-07 Nuclera Nucleics Ltd Methods of nucleic acid synthesis
US11739359B2 (en) * 2020-05-01 2023-08-29 Microsoft Technology Licensing, Llc Universal template strands for enzymatic polynucleotide synthesis
CA3178255A1 (en) * 2020-05-15 2021-11-18 Krishna KANNAN On demand synthesis of polynucleotide sequences
CA3183217A1 (en) * 2020-06-18 2021-12-23 Jonathan Cheng Compositions and methods for in situ single cell analysis using enzymatic nucleic acid extension
EP4363608B1 (en) * 2021-07-01 2025-04-02 Miltenyi Biotec B.V. & Co. KG Unit-dna composition for spatial barcoding and sequencing
EP4479551A1 (en) * 2022-02-14 2024-12-25 Miltenyi Biotec B.V. & Co. KG Direct synthesis of oligonucleotides on microtomed tissue slices
AU2023365342A1 (en) * 2022-10-20 2025-05-08 Catalog Technologies, Inc. Noise reduction for data storage in dna
GB202309649D0 (en) * 2023-06-27 2023-08-09 Oxford Nanopore Tech Plc Method
EP4530360A1 (en) * 2023-09-29 2025-04-02 Miltenyi Biotec B.V. & Co. KG Method for spatial barcoding
WO2025189419A1 (zh) * 2024-03-14 2025-09-18 深圳华大智造科技股份有限公司 全长转录本mRNA序列的获取方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011150168A1 (en) * 2010-05-28 2011-12-01 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5438131A (en) 1992-09-16 1995-08-01 Bergstrom; Donald E. 3-nitropyrrole nucleoside
US8137906B2 (en) 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
DE19925862A1 (de) 1999-06-07 2000-12-14 Diavir Gmbh Verfahren zur Synthese von DNA-Fragmenten
US6479262B1 (en) 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
EP1314783B1 (de) 2001-11-22 2008-11-19 Sloning BioTechnology GmbH Nukleinsäure-Linker und deren Verwendung in der Gensynthese
JP4663988B2 (ja) 2002-04-12 2011-04-06 ニュー・イングランド・バイオラブズ・インコーポレイティッド Ｄｎａ操作のための方法および組成物
DE10223057A1 (de) * 2002-05-24 2003-12-11 Basf Ag Verfahren zur Erzeugung von Polynukleotidmolekülen
DK1670914T3 (da) 2003-10-02 2008-05-13 Basf Se Fremgangsmåde til sekvensmætnings-mutangenese (SeSaM)
US20090006002A1 (en) 2007-04-13 2009-01-01 Sequenom, Inc. Comparative sequence analysis processes and systems
US8716190B2 (en) 2007-09-14 2014-05-06 Affymetrix, Inc. Amplification and analysis of selected targets on solid supports
WO2010025310A2 (en) 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Methods and devices for high fidelity polynucleotide synthesis
US8653832B2 (en) 2010-07-06 2014-02-18 Sharp Kabushiki Kaisha Array element circuit and active matrix device
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
US8828336B2 (en) 2011-02-02 2014-09-09 Sharp Kabushiki Kaisha Active matrix device
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
EP2807292B1 (en) 2012-01-26 2019-05-22 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
JP6375301B2 (ja) 2012-10-26 2018-08-15 オックスフォード ナノポール テクノロジーズ リミテッド 液滴界面
US9492824B2 (en) 2013-01-16 2016-11-15 Sharp Kabushiki Kaisha Efficient dilution method, including washing method for immunoassay
US9169573B2 (en) 2013-01-23 2015-10-27 Sharp Kabushiki Kaisha AM-EWOD device and method of driving with variable voltage AC driving
US9279149B2 (en) 2013-04-02 2016-03-08 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US10683536B2 (en) 2013-04-02 2020-06-16 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US9771613B2 (en) 2013-04-02 2017-09-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acid
US8808989B1 (en) * 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
CN117947149A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
CN111534504B (zh) 2014-01-22 2024-06-21 牛津纳米孔科技公开有限公司 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法
FR3020071B1 (fr) 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
US20170218416A1 (en) 2014-05-16 2017-08-03 The Regents Of The University Of California Compositions and methods for single-molecule construction of dna
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
EP3699283A1 (en) 2014-10-20 2020-08-26 Molecular Assemblies Inc. Modified template-independent enzymes for polydeoxynucleotide systhesis
GB201502152D0 (en) 2015-02-10 2015-03-25 Nuclera Nucleics Ltd Novel use
GB201503534D0 (en) 2015-03-03 2015-04-15 Nuclera Nucleics Ltd Novel method
GB201512372D0 (en) 2015-07-15 2015-08-19 Nuclera Nucleics Ltd Novel method
GB2559117B (en) 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
EP3737755B1 (en) 2018-01-12 2024-08-28 Camena Bioscience Limited Method for template-free geometric enzymatic nucleic acid synthesis
GB201801768D0 (en) 2018-02-02 2018-03-21 Oxford Nanopore Tech Ltd Synthesis method
GB2574197B (en) 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB201811810D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811813D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201913039D0 (en) 2019-09-10 2019-10-23 Oxford Nanopore Tech Ltd Polynicleotide synthesis method kit and system

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011150168A1 (en) * 2010-05-28 2011-12-01 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis

Also Published As

Publication number Publication date
EP3797170A1 (en) 2021-03-31
WO2019224544A1 (en) 2019-11-28
US12448635B2 (en) 2025-10-21
US20210198710A1 (en) 2021-07-01
AU2019274939A1 (en) 2020-11-12
GB2574197B (en) 2022-01-05
EP3797170B1 (en) 2023-07-19
JP7393351B2 (ja) 2023-12-06
GB201808474D0 (en) 2018-07-11
CN112292456A (zh) 2021-01-29
CN112292456B (zh) 2025-06-13
CA3099103A1 (en) 2019-11-28
GB2574197A (en) 2019-12-04
JP2021524246A (ja) 2021-09-13

Similar Documents

Publication Publication Date Title
AU2019274939B2 (en) Polynucleotide synthesis method, system and kit
AU2018209365B2 (en) Methods and reagents for synthesising polynucleotide molecules
AU2019307836B2 (en) Polynucleotide synthesis method, kit and system
US20250263767A1 (en) Polynucleotide synthesis method, kit and system
EP3824100B1 (en) Polynucleotide synthesis method
WO2021048545A1 (en) Polynucleotide synthesis method, kit and system

Legal Events

Date Code Title Description
HB Alteration of name in register

Owner name: OXFORD NANOPORE TECHNOLOGIES PLC

Free format text: FORMER NAME(S): OXFORD NANOPORE TECHNOLOGIES LIMITED

FGA Letters patent sealed or granted (standard patent)