GB2569965A - Improvements in or relating to amplification of nucleic acids - Google Patents

Improvements in or relating to amplification of nucleic acids Download PDF

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Publication number
GB2569965A
GB2569965A GB1800109.9A GB201800109A GB2569965A GB 2569965 A GB2569965 A GB 2569965A GB 201800109 A GB201800109 A GB 201800109A GB 2569965 A GB2569965 A GB 2569965A
Authority
GB
United Kingdom
Prior art keywords
temperature
amplification
polymerase
target
nicking
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB1800109.9A
Other languages
English (en)
Other versions
GB201800109D0 (en
Inventor
Shen Daiwei
Kraynack Bryan
Perez Victor
Provins Jarrod
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LumiraDx UK Ltd
Original Assignee
LumiraDx UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by LumiraDx UK Ltd filed Critical LumiraDx UK Ltd
Priority to GB1800109.9A priority Critical patent/GB2569965A/en
Publication of GB201800109D0 publication Critical patent/GB201800109D0/en
Priority to EP19700540.8A priority patent/EP3735474B1/en
Priority to BR112020013158-0A priority patent/BR112020013158A2/pt
Priority to CA3086320A priority patent/CA3086320C/en
Priority to CN201980007316.4A priority patent/CN111742057B/zh
Priority to PCT/GB2019/050005 priority patent/WO2019135074A1/en
Priority to ES19700540T priority patent/ES2908438T3/es
Priority to US16/956,552 priority patent/US11655496B2/en
Priority to JP2020537215A priority patent/JP7102527B2/ja
Priority to TW108100164A priority patent/TWI805672B/zh
Priority to ARP190100008A priority patent/AR114192A1/es
Publication of GB2569965A publication Critical patent/GB2569965A/en
Priority to ZA2020/03618A priority patent/ZA202003618B/en
Priority to US18/304,282 priority patent/US20240026433A1/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/307—Single strand endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/113—Modifications characterised by incorporating modified backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/131—Modifications characterised by incorporating a restriction site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/113—Time
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/137—Reactions characterised by the reaction format or use of a specific feature the purpose or use of a displacement step

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
GB1800109.9A 2018-01-04 2018-01-04 Improvements in or relating to amplification of nucleic acids Withdrawn GB2569965A (en)

Priority Applications (13)

Application Number Priority Date Filing Date Title
GB1800109.9A GB2569965A (en) 2018-01-04 2018-01-04 Improvements in or relating to amplification of nucleic acids
BR112020013158-0A BR112020013158A2 (pt) 2018-01-04 2019-01-02 Melhoras na ou em relação à amplificação de ácidos nucleicos
ES19700540T ES2908438T3 (es) 2018-01-04 2019-01-02 Mejoras en o relacionadas con la amplificación de ácidos nucleicos
JP2020537215A JP7102527B2 (ja) 2018-01-04 2019-01-02 核酸の増幅の改善又は核酸の増幅に関する改善
CA3086320A CA3086320C (en) 2018-01-04 2019-01-02 Improvements in or relating to amplification of nucleic acids
CN201980007316.4A CN111742057B (zh) 2018-01-04 2019-01-02 核酸扩增或与其相关的改进
PCT/GB2019/050005 WO2019135074A1 (en) 2018-01-04 2019-01-02 Improvements in or relating to amplification of nucleic acids
EP19700540.8A EP3735474B1 (en) 2018-01-04 2019-01-02 Improvements in or relating to amplification of nucleic acids
US16/956,552 US11655496B2 (en) 2018-01-04 2019-01-02 Amplification of nucleic acids
TW108100164A TWI805672B (zh) 2018-01-04 2019-01-03 核酸擴增中的改良或與核酸擴增相關的改良
ARP190100008A AR114192A1 (es) 2018-01-04 2019-01-03 Mejoras en, o relacionadas con, la amplificación de ácidos nucleicos
ZA2020/03618A ZA202003618B (en) 2018-01-04 2020-06-17 Improvements in or relating to amplification of nucleic acids
US18/304,282 US20240026433A1 (en) 2018-01-04 2023-04-20 Amplification of nucleic acids

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1800109.9A GB2569965A (en) 2018-01-04 2018-01-04 Improvements in or relating to amplification of nucleic acids

Publications (2)

Publication Number Publication Date
GB201800109D0 GB201800109D0 (en) 2018-02-21
GB2569965A true GB2569965A (en) 2019-07-10

Family

ID=61190347

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1800109.9A Withdrawn GB2569965A (en) 2018-01-04 2018-01-04 Improvements in or relating to amplification of nucleic acids

Country Status (11)

Country Link
US (2) US11655496B2 (https=)
EP (1) EP3735474B1 (https=)
JP (1) JP7102527B2 (https=)
CN (1) CN111742057B (https=)
AR (1) AR114192A1 (https=)
BR (1) BR112020013158A2 (https=)
ES (1) ES2908438T3 (https=)
GB (1) GB2569965A (https=)
TW (1) TWI805672B (https=)
WO (1) WO2019135074A1 (https=)
ZA (1) ZA202003618B (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
GB201915346D0 (en) * 2019-06-21 2019-12-04 Lumiradx Tech Ltd Improvements in or relating to nicking enzymes
WO2022248874A1 (en) 2021-05-27 2022-12-01 Lumiradx Uk Ltd Improvements in or relating to digestion of reaction products

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WO2011030145A1 (en) * 2009-09-08 2011-03-17 Enigma Diagnostics Limited Nucleic acid amplification employing temperature oscillation
WO2018002649A1 (en) * 2016-06-30 2018-01-04 Lumiradx Uk Ltd Improvements in or relating to nucleic acid amplification processes

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Publication number Priority date Publication date Assignee Title
US20090017453A1 (en) * 2007-07-14 2009-01-15 Maples Brian K Nicking and extension amplification reaction for the exponential amplification of nucleic acids
WO2011030145A1 (en) * 2009-09-08 2011-03-17 Enigma Diagnostics Limited Nucleic acid amplification employing temperature oscillation
WO2018002649A1 (en) * 2016-06-30 2018-01-04 Lumiradx Uk Ltd Improvements in or relating to nucleic acid amplification processes

Also Published As

Publication number Publication date
TW201930600A (zh) 2019-08-01
TWI805672B (zh) 2023-06-21
AR114192A1 (es) 2020-08-05
WO2019135074A8 (en) 2020-02-27
EP3735474A1 (en) 2020-11-11
GB201800109D0 (en) 2018-02-21
EP3735474B1 (en) 2022-01-26
CN111742057A (zh) 2020-10-02
ES2908438T3 (es) 2022-04-29
JP7102527B2 (ja) 2022-07-19
CN111742057B (zh) 2023-08-01
US11655496B2 (en) 2023-05-23
ZA202003618B (en) 2021-10-27
BR112020013158A2 (pt) 2020-12-08
US20240026433A1 (en) 2024-01-25
JP2021509814A (ja) 2021-04-08
WO2019135074A1 (en) 2019-07-11
CA3086320A1 (en) 2019-07-11
US20210292826A1 (en) 2021-09-23

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