CN111742057B - 核酸扩增或与其相关的改进 - Google Patents
核酸扩增或与其相关的改进 Download PDFInfo
- Publication number
- CN111742057B CN111742057B CN201980007316.4A CN201980007316A CN111742057B CN 111742057 B CN111742057 B CN 111742057B CN 201980007316 A CN201980007316 A CN 201980007316A CN 111742057 B CN111742057 B CN 111742057B
- Authority
- CN
- China
- Prior art keywords
- temperature
- amplification
- polymerase
- target
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/101—DNA polymerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/307—Single strand endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/113—Modifications characterised by incorporating modified backbone
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/131—Modifications characterised by incorporating a restriction site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/301—Hairpin oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/113—Time
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/137—Reactions characterised by the reaction format or use of a specific feature the purpose or use of a displacement step
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1800109.9 | 2018-01-04 | ||
| GB1800109.9A GB2569965A (en) | 2018-01-04 | 2018-01-04 | Improvements in or relating to amplification of nucleic acids |
| PCT/GB2019/050005 WO2019135074A1 (en) | 2018-01-04 | 2019-01-02 | Improvements in or relating to amplification of nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111742057A CN111742057A (zh) | 2020-10-02 |
| CN111742057B true CN111742057B (zh) | 2023-08-01 |
Family
ID=61190347
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201980007316.4A Active CN111742057B (zh) | 2018-01-04 | 2019-01-02 | 核酸扩增或与其相关的改进 |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US11655496B2 (https=) |
| EP (1) | EP3735474B1 (https=) |
| JP (1) | JP7102527B2 (https=) |
| CN (1) | CN111742057B (https=) |
| AR (1) | AR114192A1 (https=) |
| BR (1) | BR112020013158A2 (https=) |
| ES (1) | ES2908438T3 (https=) |
| GB (1) | GB2569965A (https=) |
| TW (1) | TWI805672B (https=) |
| WO (1) | WO2019135074A1 (https=) |
| ZA (1) | ZA202003618B (https=) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201611469D0 (en) | 2016-06-30 | 2016-08-17 | Lumiradx Tech Ltd | Improvements in or relating to nucleic acid amplification processes |
| GB2569965A (en) | 2018-01-04 | 2019-07-10 | Lumiradx Uk Ltd | Improvements in or relating to amplification of nucleic acids |
| GB201915346D0 (en) * | 2019-06-21 | 2019-12-04 | Lumiradx Tech Ltd | Improvements in or relating to nicking enzymes |
| WO2022248874A1 (en) | 2021-05-27 | 2022-12-01 | Lumiradx Uk Ltd | Improvements in or relating to digestion of reaction products |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005118144A1 (en) * | 2004-06-04 | 2005-12-15 | Abacus Diagnostica Oy | Temperature control of reaction vessel, system with reaction vessel, software product for system and use of system |
| CN101952459A (zh) * | 2007-07-14 | 2011-01-19 | 爱奥尼安技术公司 | 用于核酸指数式扩增的切口和延长扩增反应 |
| WO2017027835A1 (en) * | 2015-08-12 | 2017-02-16 | Circulogene Theranostics, Llc | Method of preparing cell free nucleic acid molecules by in situ amplification |
Family Cites Families (42)
| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2017821C1 (ru) | 1990-10-10 | 1994-08-15 | Анатолий Михайлович Онищенко | Способ амплификации днк и устройство для его осуществления |
| US5432065A (en) | 1993-03-30 | 1995-07-11 | United States Biochemical Corporation | Cycle sequencing with non-thermostable DNA polymerases |
| US20020025555A1 (en) | 1997-03-06 | 2002-02-28 | Janice Au-Young | GPCR diagnostic for brain cancer |
| DE69902086T2 (de) | 1998-01-27 | 2003-03-13 | Cytocell Ltd., Banbury | Verfahren zum nachweis von nukleinsäure-zielsequenzen mittels in vitro transkription von einem rna-promoter |
| ATE286981T1 (de) | 1999-09-28 | 2005-01-15 | Roche Diagnostics Gmbh | Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation |
| US20010047200A1 (en) | 1999-10-13 | 2001-11-29 | Raymond Sun | Non-foreshortening intraluminal prosthesis |
| NO314091B1 (no) | 2000-01-12 | 2003-01-27 | Biotec Pharmacon Asa | Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet |
| US6191267B1 (en) | 2000-06-02 | 2001-02-20 | New England Biolabs, Inc. | Cloning and producing the N.BstNBI nicking endonuclease |
| DE60115811T2 (de) | 2000-10-25 | 2006-08-03 | Roche Diagnostics Gmbh | Amplifizierung unter Verwendung von modifizierten Primern |
| GB0101397D0 (en) | 2001-01-19 | 2001-03-07 | Amersham Pharm Biotech Uk Ltd | Suppression of non-specific nucleic acid amplication |
| US20030211506A1 (en) * | 2001-06-01 | 2003-11-13 | Huimin Kong | N. bstnbi nicking endonuclease and methods for using endonucleases in single-stranded displacement amplification |
| US20030138800A1 (en) | 2001-07-15 | 2003-07-24 | Keck Graduate Institute | Exponential amplification of nucleic acids using nicking agents |
| AU2002217428A1 (en) | 2001-12-04 | 2003-06-17 | Bio3 Research Srl | Mixture of two partially denaturating agents for the preparation of pcr buffer solutions |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| AU2003289651A1 (en) | 2002-12-18 | 2004-07-09 | Dingbang Xu | Pcr method and application by transnormal low thermo-denaturation temperature |
| AU2004227353B2 (en) | 2003-04-01 | 2010-02-25 | Luminex Corporation | Polymerase inhibitor and method of using same |
| EP3985125A1 (en) | 2004-06-01 | 2022-04-20 | Abbott Diagnostics Scarborough, Inc. | Recombinase polymerase amplification |
| FR2878254B1 (fr) | 2004-11-22 | 2010-08-20 | Bio Rad Pasteur | Composition pour l'amplification d'acides nucleiques |
| EP1762627A1 (de) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion |
| US7537917B2 (en) | 2006-03-31 | 2009-05-26 | Collins Michael J | Microwave assisted PCR amplification of DNA |
| US20090092967A1 (en) | 2006-06-26 | 2009-04-09 | Epoch Biosciences, Inc. | Method for generating target nucleic acid sequences |
| ES2582602T3 (es) | 2007-03-28 | 2016-09-14 | Signal Diagnostics | Sistema y método de análisis de alta resolución de ácidos nucleicos para detectar variaciones de secuencia |
| US8143006B2 (en) | 2007-08-03 | 2012-03-27 | Igor Kutyavin | Accelerated cascade amplification (ACA) of nucleic acids comprising strand and sequence specific DNA nicking |
| FI20085450A0 (fi) | 2008-05-14 | 2008-05-14 | Arto Orpana | Menetelmä DNA-juosteen syntetisoimiseksi |
| US20120156728A1 (en) | 2010-12-17 | 2012-06-21 | Life Technologies Corporation | Clonal amplification of nucleic acid on solid surface with template walking |
| GB0915664D0 (en) * | 2009-09-08 | 2009-10-07 | Enigma Diagnostics Ltd | Reaction method |
| CN107739750A (zh) | 2009-09-25 | 2018-02-27 | 艾利尔圣迭戈公司 | 粗基质中的核酸的检测 |
| US9845495B2 (en) | 2010-12-10 | 2017-12-19 | Abbott Laboratories | Method and kit for detecting target nucleic acid |
| CN103608467B (zh) | 2011-04-20 | 2017-07-21 | 美飒生物技术公司 | 用于核酸的振荡扩增反应 |
| US8343752B2 (en) | 2011-05-03 | 2013-01-01 | Verdezyne, Inc. | Biological methods for preparing adipic acid |
| RU2601129C2 (ru) | 2012-04-09 | 2016-10-27 | Инвайролоджикс Инк. | Композиции и способы для количественного определения последовательности нуклеиновой кислоты в образце |
| EP2859111B1 (en) | 2012-06-08 | 2020-07-08 | Ionian Technologies, LLC | Nucleic acid amplifications |
| JP6739339B2 (ja) | 2014-01-15 | 2020-08-12 | アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ | カバーされた配列変換ｄｎａおよび検出方法 |
| ES2759337T3 (es) | 2014-10-14 | 2020-05-08 | Abbott Lab | ADN de conversión de secuencia y ADN amplificador de señal que tiene secuencias espaciadoras de poli-ADN y métodos de detección que los utilizan |
| US10604790B2 (en) | 2014-12-24 | 2020-03-31 | Abbott Laboratories | Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same |
| GB201513128D0 (en) | 2015-07-24 | 2015-09-09 | Sense Biodetection Ltd | Nucleic acid detection method |
| US10329601B2 (en) * | 2015-12-28 | 2019-06-25 | Ionian Technologies, Inc. | Nicking and extension amplification reaction (NEAR) of Streptococcus species |
| GB201611469D0 (en) * | 2016-06-30 | 2016-08-17 | Lumiradx Tech Ltd | Improvements in or relating to nucleic acid amplification processes |
| GB201701262D0 (en) | 2017-01-25 | 2017-03-08 | Sense Biodetection Ltd | Nucleic acid detection method |
| KR20200083444A (ko) | 2017-08-31 | 2020-07-08 | 아이오니안 테크놀로지스, 엘엘씨 | 호흡기 세포융합 바이러스 종의 니킹 및 연장 증폭 반응(near) |
| GB2569965A (en) | 2018-01-04 | 2019-07-10 | Lumiradx Uk Ltd | Improvements in or relating to amplification of nucleic acids |
| GB201812149D0 (en) | 2018-07-25 | 2018-09-05 | Sense Biodetection Ltd | Nucleic acid detection method |
-
2018
- 2018-01-04 GB GB1800109.9A patent/GB2569965A/en not_active Withdrawn
-
2019
- 2019-01-02 JP JP2020537215A patent/JP7102527B2/ja active Active
- 2019-01-02 WO PCT/GB2019/050005 patent/WO2019135074A1/en not_active Ceased
- 2019-01-02 US US16/956,552 patent/US11655496B2/en active Active
- 2019-01-02 EP EP19700540.8A patent/EP3735474B1/en active Active
- 2019-01-02 ES ES19700540T patent/ES2908438T3/es active Active
- 2019-01-02 CN CN201980007316.4A patent/CN111742057B/zh active Active
- 2019-01-02 BR BR112020013158-0A patent/BR112020013158A2/pt unknown
- 2019-01-03 TW TW108100164A patent/TWI805672B/zh active
- 2019-01-03 AR ARP190100008A patent/AR114192A1/es unknown
-
2020
- 2020-06-17 ZA ZA2020/03618A patent/ZA202003618B/en unknown
-
2023
- 2023-04-20 US US18/304,282 patent/US20240026433A1/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005118144A1 (en) * | 2004-06-04 | 2005-12-15 | Abacus Diagnostica Oy | Temperature control of reaction vessel, system with reaction vessel, software product for system and use of system |
| CN101952459A (zh) * | 2007-07-14 | 2011-01-19 | 爱奥尼安技术公司 | 用于核酸指数式扩增的切口和延长扩增反应 |
| CN104357546A (zh) * | 2007-07-14 | 2015-02-18 | 爱奥尼安技术公司 | 用于核酸指数式扩增的切口和延长扩增反应 |
| WO2017027835A1 (en) * | 2015-08-12 | 2017-02-16 | Circulogene Theranostics, Llc | Method of preparing cell free nucleic acid molecules by in situ amplification |
Non-Patent Citations (1)
| Title |
|---|
| Scope and limitations of the nicking enzyme amplification reaction for the synthesis of base-modified oligonucleotides and primers for PCR;Petra Ménová;《Bioconjug Chem》;20130619;第24卷(第6期);1081-1093 * |
Also Published As
| Publication number | Publication date |
|---|---|
| TW201930600A (zh) | 2019-08-01 |
| TWI805672B (zh) | 2023-06-21 |
| AR114192A1 (es) | 2020-08-05 |
| WO2019135074A8 (en) | 2020-02-27 |
| EP3735474A1 (en) | 2020-11-11 |
| GB201800109D0 (en) | 2018-02-21 |
| EP3735474B1 (en) | 2022-01-26 |
| CN111742057A (zh) | 2020-10-02 |
| ES2908438T3 (es) | 2022-04-29 |
| JP7102527B2 (ja) | 2022-07-19 |
| US11655496B2 (en) | 2023-05-23 |
| ZA202003618B (en) | 2021-10-27 |
| BR112020013158A2 (pt) | 2020-12-08 |
| GB2569965A (en) | 2019-07-10 |
| US20240026433A1 (en) | 2024-01-25 |
| JP2021509814A (ja) | 2021-04-08 |
| WO2019135074A1 (en) | 2019-07-11 |
| CA3086320A1 (en) | 2019-07-11 |
| US20210292826A1 (en) | 2021-09-23 |
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