GB2562858B - Method for the cytometric analysis of cell samples - Google Patents

Method for the cytometric analysis of cell samples Download PDF

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Publication number
GB2562858B
GB2562858B GB1804709.2A GB201804709A GB2562858B GB 2562858 B GB2562858 B GB 2562858B GB 201804709 A GB201804709 A GB 201804709A GB 2562858 B GB2562858 B GB 2562858B
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United Kingdom
Prior art keywords
cytometric analysis
cell samples
samples
cytometric
cell
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GB1804709.2A
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GB201804709D0 (en
GB2562858A (en
Inventor
Woerdemann Mike
Diepenbrock Stefan
Höfling Sascha
Krüger Daniel
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Evident Technology Center Europe GmbH
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Olympus Soft Imaging Solutions GmbH
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Publication of GB2562858A publication Critical patent/GB2562858A/en
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    • G01—MEASURING; TESTING
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    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
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    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
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    • G01N15/1023—Microstructural devices for non-optical measurement
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    • G16H40/00—ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices
    • G16H40/60—ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices
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    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G—PHYSICS
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G—PHYSICS
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    • G—PHYSICS
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    • G01N2015/1024—Counting particles by non-optical means
    • G—PHYSICS
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    • G01N2015/144—Imaging characterised by its optical setup
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    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
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    • G—PHYSICS
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    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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  • Health & Medical Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
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  • General Physics & Mathematics (AREA)
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  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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  • General Business, Economics & Management (AREA)
  • Business, Economics & Management (AREA)
  • Theoretical Computer Science (AREA)
  • Multimedia (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
GB1804709.2A 2017-04-05 2018-03-23 Method for the cytometric analysis of cell samples Active GB2562858B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102017107348.5A DE102017107348B4 (de) 2017-04-05 2017-04-05 Verfahren zur zytometrischen Analyse von Zellproben

Publications (3)

Publication Number Publication Date
GB201804709D0 GB201804709D0 (en) 2018-05-09
GB2562858A GB2562858A (en) 2018-11-28
GB2562858B true GB2562858B (en) 2022-11-30

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GB1804709.2A Active GB2562858B (en) 2017-04-05 2018-03-23 Method for the cytometric analysis of cell samples

Country Status (5)

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US (2) US11023705B2 (https=)
JP (1) JP6790013B2 (https=)
CN (1) CN108693098B (https=)
DE (1) DE102017107348B4 (https=)
GB (1) GB2562858B (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102017107348B4 (de) * 2017-04-05 2019-03-14 Olympus Soft Imaging Solutions Gmbh Verfahren zur zytometrischen Analyse von Zellproben
CN110057729A (zh) * 2019-03-12 2019-07-26 天津大学 一种人体颗粒物被动沉降浓度测试方法
CN110132823A (zh) * 2019-05-15 2019-08-16 林伟阳 一种基于模板匹配与查找轮廓的细胞计数方法
JP7549647B2 (ja) * 2019-08-19 2024-09-11 ザルトリウス バイオアナリティカル インストゥルメンツ， インコーポレイテッド サンプル間の分離ガス検出のためのシステムおよび方法
CN112819795B (zh) * 2021-02-05 2023-03-31 复旦大学附属中山医院 基于多色荧光图片的细胞数量和面积的统计方法
CN113916751B (zh) * 2021-09-15 2024-02-09 中国人民解放军总医院第一医学中心 微视图像全细胞自动检测系统
FR3144286A1 (fr) 2022-12-27 2024-06-28 Commissariat A L'energie Atomique Et Aux Energies Alternatives Dispositif et procédé d’observation d’une fluorescence ou d’une luminescence d’une particule mobile
CN120953263B (zh) * 2025-10-13 2025-12-23 陕西翱港智能科技有限公司 一种器官芯片内皮屏障完整性动态评估方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6169816B1 (en) * 1997-05-14 2001-01-02 Applied Imaging, Inc. Identification of objects of interest using multiple illumination schemes and finding overlap of features in corresponding multiple images
US6615141B1 (en) * 1999-05-14 2003-09-02 Cytokinetics, Inc. Database system for predictive cellular bioinformatics
US20060068371A1 (en) * 1999-01-25 2006-03-30 Amnis Corporation Methods for analyzing inter-cellular phenomena
US20090197272A1 (en) * 2006-06-29 2009-08-06 Hendriks Bernardus Hendrikus W Vitro method of detecting and/or diagnosing cancer using uv light based dna image cytometry
US20140030729A1 (en) * 1999-01-25 2014-01-30 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
WO2017214023A1 (en) * 2016-06-10 2017-12-14 Amnis Corporation A method to combine brightfield and fluorescent channels for cell image segmentation and morphological analysis using images obtained from imaging flow cytometer (ifc)

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4661913A (en) * 1984-09-11 1987-04-28 Becton, Dickinson And Company Apparatus and method for the detection and classification of articles using flow cytometry techniques
JPH0325354A (ja) * 1989-06-22 1991-02-04 Toshiba Corp 自動蛍光分析装置
JP3121849B2 (ja) 1991-02-27 2001-01-09 シスメックス株式会社 フローイメージサイトメータ
US5548661A (en) * 1991-07-12 1996-08-20 Price; Jeffrey H. Operator independent image cytometer
US6259807B1 (en) * 1997-05-14 2001-07-10 Applied Imaging Corp. Identification of objects of interest using multiple illumination schemes and finding overlap of features in corresponding multiple images
DE19754909C2 (de) 1997-12-10 2001-06-28 Max Planck Gesellschaft Verfahren und Vorrichtung zur Erfassung und Bearbeitung von Abbildungen biologischen Gewebes
JP3113909B2 (ja) 1997-12-22 2000-12-04 株式会社日本水処理技研 菌類の即時判別装置
AU2896299A (en) 1998-03-04 1999-09-20 Board Of Regents, The University Of Texas System Optical stretcher
US6249341B1 (en) 1999-01-25 2001-06-19 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
US8406498B2 (en) * 1999-01-25 2013-03-26 Amnis Corporation Blood and cell analysis using an imaging flow cytometer
US20020146734A1 (en) * 2001-02-21 2002-10-10 Amnis Corporation Method and apparatus for labeling and analyzing cellular components
CN2530263Y (zh) * 2002-03-08 2003-01-08 中国科学院上海光学精密机械研究所 检测生物荧光的光纤传感器
JP4752208B2 (ja) 2004-07-23 2011-08-17 株式会社ニコン 光学顕微鏡システムおよびこれを用いた試料動画像生成方法
WO2008005426A2 (en) * 2006-06-30 2008-01-10 University Of South Florida Computer-aided pathological diagnosis system
JP4937850B2 (ja) 2007-07-03 2012-05-23 オリンパス株式会社 顕微鏡システム、そのｖｓ画像生成方法、プログラム
JP5407015B2 (ja) * 2008-07-10 2014-02-05 国立大学法人徳島大学 画像処理装置、画像処理方法、コンピュータ実行可能な画像処理プログラム、及び顕微鏡システム
DE102008059788B4 (de) * 2008-12-01 2018-03-08 Olympus Soft Imaging Solutions Gmbh Analyse und Klassifizierung insbesondere biologischer oder biochemischer Objekte auf Basis von Zeitreihen-Bildern, anwendbar bei der zytometrischen Time-Lapse-Zellanalyse in der bildbasierten Zytometrie
US8849006B2 (en) 2009-03-25 2014-09-30 Quorum Technologies Inc. Darkfield imaging system and methods for automated screening of cells
EP2601523B1 (en) * 2010-08-05 2018-06-06 Abbott Point Of Care, Inc. Method and apparatus for automated whole blood sample analyses from microscopy images
US8941062B2 (en) * 2010-11-16 2015-01-27 1087 Systems, Inc. System for identifying and sorting living cells
DE102011075369B4 (de) 2011-05-05 2022-02-17 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zur Objektabbildung
JP5734758B2 (ja) * 2011-06-16 2015-06-17 オリンパス株式会社 レーザー顕微鏡
US9632301B2 (en) 2011-09-21 2017-04-25 Huron Technologies International Inc. Slide scanner with a tilted image
WO2014152048A2 (en) * 2013-03-14 2014-09-25 Cytonome/St, Llc Assemblies and methods for reducing optical crosstalk in particle processing systems
US20140334696A1 (en) * 2013-05-13 2014-11-13 Optra Systems Inc. Cloud-based method and system for digital pathology
US9779500B2 (en) * 2013-07-03 2017-10-03 Konica Minolta, Inc. Image processing device, pathological diagnosis support system, image processing program, and pathological diagnosis support method
JP6454478B2 (ja) * 2013-09-12 2019-01-16 オリンパス株式会社 心筋細胞への分化をモニタリングする方法
US10041949B2 (en) * 2013-09-13 2018-08-07 The Board Of Trustees Of The Leland Stanford Junior University Multiplexed imaging of tissues using mass tags and secondary ion mass spectrometry
WO2015063613A2 (en) * 2013-11-01 2015-05-07 Spherium Biomed S.L. Inclusion bodies for transdermal delivery of therapeutic and cosmetic agents
JP6146265B2 (ja) 2013-11-07 2017-06-14 ソニー株式会社 顕微鏡システムおよびオートフォーカス方法
CA2932892C (en) * 2014-01-28 2020-09-22 Ventana Medical Systems, Inc. Adaptive classification for whole slide tissue segmentation
WO2016153819A1 (en) * 2015-03-25 2016-09-29 The Board Of Trustees Of The Leland Stanford Junior University Single cell analysis using secondary ion mass spectrometry
CN107635548B (zh) * 2015-05-16 2020-09-01 学校法人几德学园 利用突变型伴侣蛋白复合体的细胞内局部性药物递送系统用纳米胶囊
JP7146635B2 (ja) * 2015-12-16 2022-10-04 ヴェンタナ メディカル システムズ， インク． マルチスペクトル軌跡を使用するデジタル撮像のための自動合焦の方法およびシステム
JPWO2017169083A1 (ja) * 2016-03-31 2019-02-07 富士フイルム株式会社 画像撮像装置及び画像撮像方法
DE102017107348B4 (de) * 2017-04-05 2019-03-14 Olympus Soft Imaging Solutions Gmbh Verfahren zur zytometrischen Analyse von Zellproben

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6169816B1 (en) * 1997-05-14 2001-01-02 Applied Imaging, Inc. Identification of objects of interest using multiple illumination schemes and finding overlap of features in corresponding multiple images
US20060068371A1 (en) * 1999-01-25 2006-03-30 Amnis Corporation Methods for analyzing inter-cellular phenomena
US20140030729A1 (en) * 1999-01-25 2014-01-30 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US6615141B1 (en) * 1999-05-14 2003-09-02 Cytokinetics, Inc. Database system for predictive cellular bioinformatics
US20090197272A1 (en) * 2006-06-29 2009-08-06 Hendriks Bernardus Hendrikus W Vitro method of detecting and/or diagnosing cancer using uv light based dna image cytometry
WO2017214023A1 (en) * 2016-06-10 2017-12-14 Amnis Corporation A method to combine brightfield and fluorescent channels for cell image segmentation and morphological analysis using images obtained from imaging flow cytometer (ifc)

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CN108693098B (zh) 2021-08-10
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