GB2445706A - Reprogramming and genetic modification of cells - Google Patents

Reprogramming and genetic modification of cells Download PDF

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Publication number
GB2445706A
GB2445706A GB0808244A GB0808244A GB2445706A GB 2445706 A GB2445706 A GB 2445706A GB 0808244 A GB0808244 A GB 0808244A GB 0808244 A GB0808244 A GB 0808244A GB 2445706 A GB2445706 A GB 2445706A
Authority
GB
United Kingdom
Prior art keywords
cell
cells
reprogramming
genetic modification
fusing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0808244A
Other languages
English (en)
Other versions
GB0808244D0 (en
Inventor
Ian Chambers
Jose Rebero Da Silva
Austin Gerard Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Edinburgh
Original Assignee
University of Edinburgh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0523039A external-priority patent/GB0523039D0/en
Priority claimed from GB0605998A external-priority patent/GB0605998D0/en
Application filed by University of Edinburgh filed Critical University of Edinburgh
Publication of GB0808244D0 publication Critical patent/GB0808244D0/en
Publication of GB2445706A publication Critical patent/GB2445706A/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/605—Nanog
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Developmental Biology & Embryology (AREA)
  • Transplantation (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
GB0808244A 2005-11-11 2006-11-13 Reprogramming and genetic modification of cells Withdrawn GB2445706A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0523039A GB0523039D0 (en) 2005-11-11 2005-11-11 Reprogramming and genetic modification of cells
GB0605998A GB0605998D0 (en) 2006-03-24 2006-03-24 Reprogramming And Genetic Modification Of Cells
PCT/GB2006/004218 WO2007054720A1 (en) 2005-11-11 2006-11-13 Reprogramming and genetic modification of cells

Publications (2)

Publication Number Publication Date
GB0808244D0 GB0808244D0 (en) 2008-06-11
GB2445706A true GB2445706A (en) 2008-07-16

Family

ID=37763882

Family Applications (2)

Application Number Title Priority Date Filing Date
GB0808244A Withdrawn GB2445706A (en) 2005-11-11 2006-11-13 Reprogramming and genetic modification of cells
GB0702261A Withdrawn GB2434155A (en) 2005-11-11 2007-02-06 Reprogramming and genetic modification of cells

Family Applications After (1)

Application Number Title Priority Date Filing Date
GB0702261A Withdrawn GB2434155A (en) 2005-11-11 2007-02-06 Reprogramming and genetic modification of cells

Country Status (6)

Country Link
EP (1) EP1957643A2 (enExample)
JP (1) JP2009515515A (enExample)
AU (1) AU2006313518A1 (enExample)
GB (2) GB2445706A (enExample)
TW (1) TW200730623A (enExample)
WO (1) WO2007054720A1 (enExample)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
US8129187B2 (en) 2005-12-13 2012-03-06 Kyoto University Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2
CN101864392B (zh) 2005-12-13 2016-03-23 国立大学法人京都大学 核重新编程因子
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
GB0701062D0 (en) * 2007-01-19 2007-02-28 Evocell Ltd Biological materials and uses thereof
US20100184051A1 (en) * 2007-05-30 2010-07-22 The General Hospital Corporation Methods of generating pluripotent cells from somatic cells
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
US9213999B2 (en) 2007-06-15 2015-12-15 Kyoto University Providing iPSCs to a customer
EP2090649A1 (en) 2008-02-13 2009-08-19 Fondazione Telethon Method for reprogramming differentiated cells
JP5951254B2 (ja) * 2008-03-17 2016-07-13 ザ スクリプス リサーチ インスティテュート 人工多能性幹細胞を作製するための化学的手法と遺伝的手法の組み合わせ法
AU2015201026B2 (en) * 2008-03-17 2017-03-16 The Scripps Research Institute Combined chemical and genetic approaches for generation of induced pluripotent stem cells
CN101855350B (zh) 2008-05-02 2014-12-31 国立大学法人京都大学 核重编程序方法
WO2010017562A2 (en) 2008-08-08 2010-02-11 Mayo Foundation For Medical Education And Research Induced pluripotent stem cells
JP5645197B2 (ja) * 2009-06-23 2014-12-24 学校法人日本大学 幹細胞の未分化状態を維持する新規方法
US20130029416A1 (en) 2011-07-22 2013-01-31 Tayaramma Thatava Differentiating induced pluripotent stem cells into glucose-responsive, insulin-secreting progeny
WO2016168658A1 (en) * 2015-04-15 2016-10-20 University Of Massachusetts Compositions and methods for xi chromosome reactivation
CA3038721A1 (en) 2016-09-30 2018-04-05 Mayo Foundation For Medical Education And Research Viral vectors for nuclear reprogramming

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol. 313, 2004, U Yoshida-Koide et al, "Involvement of Ras in extraembryonic endoderm differentiation of embryonic stem cells.", pages 475-481 *
CELL, Vol. 113, 2003, I Chambers et al, "Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells" pages 643-655 *
CLONING AND STEM CELLS, vol. 6, 2004, I Chambers, "The molecular basis of pluripotency in mouse embryonic stem cells" pages 386-391 *
GASTROENTEROLOGY, Vol. 126, Suppl. 2, 2004, X-L Kuai et al, "Treatment of surgically induced acute liver failure by transplantation of HNF4-overexpression embryonic stem cells" page A753, *
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, Vol. 7, 2003, S Constantinescu, "Stemness, fusion and renewal of hematopoietic and embryonic stem cells." pages 103-112 *
MECHANISMS OF DEVELOPMENT, Vol. 122, 2005, S-Y Hatano et al, "Pluripotential competence of cells associated with Nanog activity" pages 67-79 *
NATURE, Vol. 441, 2006 J Silva et al, "Nanog promotes transfer of pluripotency after cell fusion", pages 997-1001 *
REPRODUCTION FERTILITY AND DEVELOPMENT, Vol. 17, 2005, T Do Jeong et al, "Comparison of neurosphere cells with cumulus cells after fusion with embryonic stem cells: reprogramming potential" , pages 143-149 *
SCIENCE, Vol. 309, 2005, C Cowan et al, "Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells.", pages 1369-1373 *

Also Published As

Publication number Publication date
JP2009515515A (ja) 2009-04-16
AU2006313518A1 (en) 2007-05-18
EP1957643A2 (en) 2008-08-20
GB2434155A (en) 2007-07-18
TW200730623A (en) 2007-08-16
GB0702261D0 (en) 2007-03-14
WO2007054720A8 (en) 2008-11-13
WO2007054720A1 (en) 2007-05-18
AU2006313518A2 (en) 2008-06-26
GB0808244D0 (en) 2008-06-11

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