GB0808244D0 - Reprogramming and genetic modification of cells - Google Patents

Reprogramming and genetic modification of cells

Info

Publication number
GB0808244D0
GB0808244D0 GBGB0808244.8A GB0808244A GB0808244D0 GB 0808244 D0 GB0808244 D0 GB 0808244D0 GB 0808244 A GB0808244 A GB 0808244A GB 0808244 D0 GB0808244 D0 GB 0808244D0
Authority
GB
United Kingdom
Prior art keywords
cell
cells
reprogramming
genetic modification
fusing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GBGB0808244.8A
Other versions
GB2445706A (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Edinburgh
Original Assignee
University of Edinburgh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0523039A external-priority patent/GB0523039D0/en
Priority claimed from GB0605998A external-priority patent/GB0605998D0/en
Application filed by University of Edinburgh filed Critical University of Edinburgh
Publication of GB0808244D0 publication Critical patent/GB0808244D0/en
Publication of GB2445706A publication Critical patent/GB2445706A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/605—Nanog
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Abstract

Methods for reprogramming and optional genetic modification of cells are provided. A pluripotent genome is obtained from a differentiated genome by fusing a pluripotent cell with a differentiated cell in the presence of Nanog or a MEK inhibitor. A cell is genetically modified by providing first and second cells, each containing chromosomes, fusing the first cell and the second cell, and culturing the fused cell so as to obtain a diploid cell containing at least one chromosome from the first cell and at least one chromosome form the second cell. A method of cell fusion comprises fusing a first cell and a second cell in the presence of Nanog or a MEK inhibitor. Cells obtained thereby and their uses axe also described.
GB0808244A 2005-11-11 2006-11-13 Reprogramming and genetic modification of cells Withdrawn GB2445706A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0523039A GB0523039D0 (en) 2005-11-11 2005-11-11 Reprogramming and genetic modification of cells
GB0605998A GB0605998D0 (en) 2006-03-24 2006-03-24 Reprogramming And Genetic Modification Of Cells
PCT/GB2006/004218 WO2007054720A1 (en) 2005-11-11 2006-11-13 Reprogramming and genetic modification of cells

Publications (2)

Publication Number Publication Date
GB0808244D0 true GB0808244D0 (en) 2008-06-11
GB2445706A GB2445706A (en) 2008-07-16

Family

ID=37763882

Family Applications (2)

Application Number Title Priority Date Filing Date
GB0808244A Withdrawn GB2445706A (en) 2005-11-11 2006-11-13 Reprogramming and genetic modification of cells
GB0702261A Withdrawn GB2434155A (en) 2005-11-11 2007-02-06 Reprogramming and genetic modification of cells

Family Applications After (1)

Application Number Title Priority Date Filing Date
GB0702261A Withdrawn GB2434155A (en) 2005-11-11 2007-02-06 Reprogramming and genetic modification of cells

Country Status (6)

Country Link
EP (1) EP1957643A2 (en)
JP (1) JP2009515515A (en)
AU (1) AU2006313518A1 (en)
GB (2) GB2445706A (en)
TW (1) TW200730623A (en)
WO (1) WO2007054720A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4223769A3 (en) 2005-12-13 2023-11-01 Kyoto University Nuclear reprogramming factor
US8129187B2 (en) 2005-12-13 2012-03-06 Kyoto University Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
GB0701062D0 (en) * 2007-01-19 2007-02-28 Evocell Ltd Biological materials and uses thereof
US20100184051A1 (en) * 2007-05-30 2010-07-22 The General Hospital Corporation Methods of generating pluripotent cells from somatic cells
JP2008307007A (en) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag Human pluripotent stem cell induced from human tissue-originated undifferentiated stem cell after birth
US9213999B2 (en) 2007-06-15 2015-12-15 Kyoto University Providing iPSCs to a customer
EP2090649A1 (en) 2008-02-13 2009-08-19 Fondazione Telethon Method for reprogramming differentiated cells
AU2015201026B2 (en) * 2008-03-17 2017-03-16 The Scripps Research Institute Combined chemical and genetic approaches for generation of induced pluripotent stem cells
SG10201607710UA (en) * 2008-03-17 2016-11-29 Scripps Research Inst Combined chemical and genetic approaches for generation of induced pluripotent stem cells
EP2268809B1 (en) 2008-05-02 2019-02-06 Kyoto University Method of nuclear reprogramming
US10047346B2 (en) 2008-08-08 2018-08-14 Mayo Foundation For Medical Education And Research Method of treating heart tissue using induced pluripotent stem cells
JP5645197B2 (en) * 2009-06-23 2014-12-24 学校法人日本大学 A novel method for maintaining the undifferentiated state of stem cells
US20130029416A1 (en) 2011-07-22 2013-01-31 Tayaramma Thatava Differentiating induced pluripotent stem cells into glucose-responsive, insulin-secreting progeny
EP3283127B1 (en) 2015-04-15 2020-02-26 University of Massachusetts Compositions and methods for xi chromosome reactivation

Also Published As

Publication number Publication date
WO2007054720A8 (en) 2008-11-13
GB2434155A (en) 2007-07-18
GB0702261D0 (en) 2007-03-14
TW200730623A (en) 2007-08-16
AU2006313518A1 (en) 2007-05-18
EP1957643A2 (en) 2008-08-20
WO2007054720A1 (en) 2007-05-18
GB2445706A (en) 2008-07-16
JP2009515515A (en) 2009-04-16
AU2006313518A2 (en) 2008-06-26

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Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)