GB2387902A - Spore trap - Google Patents

Spore trap Download PDF

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Publication number
GB2387902A
GB2387902A GB0209148A GB0209148A GB2387902A GB 2387902 A GB2387902 A GB 2387902A GB 0209148 A GB0209148 A GB 0209148A GB 0209148 A GB0209148 A GB 0209148A GB 2387902 A GB2387902 A GB 2387902A
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GB
United Kingdom
Prior art keywords
spore
trap
sampling chamber
rotatable
fan unit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0209148A
Other versions
GB0209148D0 (en
Inventor
Bryan Granville Wili
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BURKARD SCIENT
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BURKARD SCIENT
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BURKARD SCIENT filed Critical BURKARD SCIENT
Priority to GB0209148A priority Critical patent/GB2387902A/en
Publication of GB0209148D0 publication Critical patent/GB0209148D0/en
Publication of GB2387902A publication Critical patent/GB2387902A/en
Withdrawn legal-status Critical Current

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Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/22Devices for withdrawing samples in the gaseous state
    • G01N1/2202Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/22Devices for withdrawing samples in the gaseous state
    • G01N1/2202Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling
    • G01N2001/222Other features
    • G01N2001/2223Other features aerosol sampling devices
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/22Devices for withdrawing samples in the gaseous state
    • G01N1/24Suction devices
    • G01N2001/245Fans
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/2833Collecting samples on a sticky, tacky, adhesive surface

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

A spore trap 1 e.g. for fungal spores or pollen capture, has a rotatable sampling chamber 2 connected a to a wind vane 3 and includes a fan unit for intake of air samples through an orifice nozzle 5. Housed within the chamber is a motor driven spore sample collector to receive and collect spore intake via the nozzle over a selected time period. Typically the collector is in the form of an adhesive coated disc disposed in a plane orthogonal to the direction of incoming air and also the fan unit freely rotates with the chamber in response to the movement of the vane. A plurality of traps may be in an array and powered by a solar panel. The trap may be on a tripod 7.

Description

r SPORE TRAP
This invention relates to aero-biological field apparatus. In particular, it relates to
volumetric spore traps.
Spore haps may be known as spore samplers.
Spore traps are used widely in epidemiological studies for collecting airborne fungal spores, and in other agricultural and forestry applications where fungal spores or pollen 10 needs to be collected for species identification, count levels or other such testing programmes. ... A previously proposed spore trap, as shown in Figure 6, comprises a rotatable housing 100, to which a wind vane 110 is connected. Beneath the housing 100, and 15 connected to it via a throat section 105, is a vacuum pump motor unit 120 and support legs 121 to 123 (third leg 123 is hidden from view). Also shown in the figure is a power cable 124; this is conventionally connected to a DC battery supply (not shown).
During operation, a pump housed within the vacuum pump motor unit is activated 20 to extract air from within the housing 100, causing an inflow of external air through the orifice nozzle 101. The flow rate ofthe intake air is typically 10 litres per minute, and the orifice aperture is usually 14rnm x 2mm, although other sizes can be employed as necessary to trap particles typically in the range lam to loran in diameter. The nozzle 101 is protected from the elements by a visor 102, and access to the interior of the housing 100 is 25 achieved by removal of a lid assembly 103 via a locking handle 104.
The wind vane 110 operates in a known manner to orientate the orifice nozzle 101 to face upwind by rotation of both the housing 100 and throat section 105 about a central axis.
t r - 2 Located within the housing 100 (see Figures 7A and 7B) is a clockwork-driven drum 130. The drum 130 can either support an adhesivecoated transparent plastic tape device 131 (Figure 7A) or it can be used as a cam to drive a reciprocating plate assembly 132 (Figure 7B). In the case of Figure 7A, the drum rotates the tape device 131 through 5 360 over a period of 7 days (168 hours). During this time spores entering the housing lOO through the orifice nozzle 101 will adhere to the adhesive of the tape as the drum rotates, thereby giving a physical record of spore concentrations in the vicinity of the spore trap over the given period.
10 Figure 7B shows an arrangement for a 24 hour test period. The drum moves the plate assembly, to which a glass slide including adhesive tape is attached (not shown), past the orifice nozzle 101; the speed of the drum 100 and the length of the slide being such that it takes a full day for the slide to traverse past the orifice. Again, any spores entering the housing 100 during this time will become affixed to the adhesive tape and will provide a 15 physical record of spore concentration fluctuations etc. during the test period.
There are many disadvantages associated with conventional spore traps: they are bulky and heavy making transportation and set-up difficult; the wind vane cannot be adjusted to suit different wind conditions; preparation of the spore trapping surface using 20 adhesive and Melinex plastic tape is time consuming, as is the post-test procedure of dismounting the tape and segregating it into hour-sections on a cutting block; the large vacuum pump motor unit requires mains voltage or mains voltage level (240V) batteries to operate; the vertical height of the orifice nozzle from the ground cannot be adjusted to suit various test situations; and the drum is clockwork-driven and must therefore be wound prior 25 to use, which also limits the test periods to either 1 day or 7.
The present invention arose from attempt to solve some or all of the above mentioned disadvantages.
30 According to an aspect of the present invention, there is provided a tripod
l - 3 mountable, sport trap comprising: a rotatable sampling chamber connected to a wind vane, the sampling chamber including a fan unit for the intake of external air samples through an orifice nozzle; a rotatable, electric motor driven sample collector housed within the sampling chamber, adapted to receive and collect spore intake through the orifice nozzle 5 over a user selectable time period; wherein the sample collector is a rotatable disc disposed in a plane substantially orthogonal to the direction of incoming external air.
According to a further aspect of the present invention there is provided a tripod-
mountable, spore trap comprising: a rotatable sampling chamber connected to a wind vane, 10 the sampling chamber including a fan unit for the intake of external a* samples through an orifice nozzle; a rotatable, electric motor driven sample collector housed within the sampling chamber, adapted to receive and collect spore intake through the orifice nozzle over a user selectable time period; wherein the fan unit is integral to the sampling chamber and is disposed therein so as to freely rotate with the sampling chamber in response to the 15 operation of the wind vane.
Preferably, the sample collector is a rotatable disc disposed in a plane substantially orthogonal to the direction of incoming external air.
20 Preferably, the rotatable disc includes time period graduations.
Preferably, the interconnection between the sample chamber and the wind vane is telescopic. 25 Preferably, the fan unit and the electric motor require substantially no more than 12V (DC) to operate.
Preferably, the spore trap includes control means for controlling the fan unit and the electric motor. Preferably, the control means is an electronic control means.
-4 Preferably, the electronic control means includes an automatic shutdown function for halting further spore sampling by stopping the motor and the fan unit at a desired time position. 5 Preferably, the tripod for mounting the spore trap includes telescopic legs.
According to another aspect of the present invention there is provided an array of portable, tripod-mountable, spore traps each spore trap comprising: a rotatable sampling chamber connected to a wind vane, the sampling chamber including a fan unit for the intake 10 of external air samples through an orifice nozzle; a rotatable, electric motor driven sample collector housed within the sampling chamber, adapted to receive and collect spore intake through the orifice nozzle over a user selectable time period; wherein the array of spore traps are powered by a solar panel.
15 According to a further aspect of the present invention, there is provided a spore trap, comprising any one or more of the novel features described herein.
Embodiments of the present invention will now be described, by way of example only, with reference to the accompanying schematic drawings, in which: 20 Figure 1 shows an embodiment of the spore trap of the present invention mounted upon a tripod; Figure 2 is a perspective view of an embodiment of the spore trap; Figure 3 is a perspective view of an embodiment of the spore trap showing the sampling chamber door in an open position; 25 Figure 4A is a sampling disc for up to a 24 hour test period; Figure 4B is a sampling disc for up to a 7day test period; Figure 5 shows an array of the spore traps of Figure 1 in electrical connection with a solar panel; Figure 6 shows a previously proposed spore trap; 30 Figure 7A shows an internal sectional view of the housing of the spore trap of
- 5 Figure 6 including a drum-type trap assembly; and Figure 7B shows an internal sectional view of the housing of the spore trap of Figure 6 including a slide-type trap assembly.
5 Referring to Figures 1 and 2, a spore trap 1 comprises a sampling chamber 2 connected to a wind vane 3 via an interconnecting member 4. The interconnecting member 4 may be rigid and of a fixed length (as shown), or it may be telescopic to accommodate differing test situations.
10 On the opposing end of the sampling chamber 2 from the wind vane 3 is a sampling chamber door 12 and a protective visor 8. The sampling chamber door 12 conceals a sampling disc 13 (see Figure 3). The protective visor 8, which is removably secured to the sampling chamber 12 via a fastening mechanism 9, provides a degree of protection from the elements and the indigenous wildlife of the test site. The sampling chamber door 12 is 15 releasably locked in place via locking mechanism 11; this can be of any known suitable form of locking mechanism.
Below the protective visor 8, and disposed on the sampling chamber door 12 is an air intake orifice nozzle 5. The orifice nozzle includes an elongated orifice 10 that is 20 preferably 14mm x 2mm in dimension. However, other dimensions may be employed to suit the particular requirements of the test situation.
Situated below the sampling chamber 2, and attached to it, is a throat section 6.
This section is adapted to releasably connect with a tripod 7, that preferably includes 25 telescopic legs; this facilitates overall height adjustment of the spore trap 1. In addition, the throat section 6 incorporates a bearing housing which provides for 360 rotation about the apex of the tripod, thus allowing the spore trap to align itself into the wind during operation. 30 Referring to Figure 3, the sampling chamber door 12 is depicted in an open position
- 6 revealing the sampling disc 13 which is disposed directly behind the orifice 10 (when the door is closed) at a distance of 0.06cm from the orifice. The disc 13, which is preferably 12cm in diameter (the size of a standard optical disc such as a CD, DVD, etc), is fixed at its centre 14 and is rotatable about this point. Behind, and located within the sampling 5 chamber 2 is a DC stepper motor 15 (not shown) that drives the rotation of the disc 13.
Located behind the motor 15 is an impeller-type fan system 16 (also not shown). This provides an adjustable intake flow rate (typically around 0. 167 litres per second) and is powered by the same 12V supply that provides power for the motor 15. The typical power consumption is less than SW.
In a preferred embodiment, the spore trap 1 includes an electronic control unit (not shown) for controlling the motor 15 and the fan system 16. The control functions of the control unit may include, for example: an adjustable operating period for the disc, that is to say a user will be able to set the angular speed of the motor to determine the time period for 15 one complete revolution; an abort test function; an automatic shut-down at the end of the test period function etc. The electronic control unit is preferably located within the sampling chamber 2 and will include an indicator, such as an LED, for indicating, for example, that the spore trap is in operation, or that a test run is being undertaken.
20 The system may have 16 user-selectable time periods, for example.
In operation, and with reference to Figures 2 to 4B, a user will typically select disc 17 for a 24 hour (or less) test or disc 18 for a 7 day (or less) test. The discs include graduations 19, 20 for segregating the test period into separate time slots, and are 25 transparent which facilitates easy post-test inspection via optical transmission microscopy or the like.
The discs are generally pre-coated with an adhesive, such as 'Gelvatol', although in certain situations the user may apply an adhesive or coating of choice onto a blank disc.
Once this disc has been mounted inside the spore trap, and the sampling chamber door 12 closed, a user will set the required test period via the electronic control unit. Once the spore trap is activated, the fan system and motor will begin to operate; providing air intake through the orifice 10 and rotating the disc 13 respectively. At the end of the test S period, a user will unlock the sampling chamber door 12 via locking mechanism 11 and remove the sample disc 13 to a remote location for examination of spore impacts.
Automatic shutdown enables rotation of the disk and stopping of the fan, to enable an operator to visit the instrument at his convenience, without fear that the test will have overrun, and perhaps overwritten data on the disk by rotating the disc through more than 10 360 .
Figure 5 shows an array of three spore traps each connected to a respective battery supply 21. Each of the battery supplies 21 is connected in turn to a central solar panel 22.
The solar panel 22 provides an energy source for maintenance of correct voltage level IS within the respective battery supplies 21. More, or less, than three traps maybe powered by a single solar panel, or array of solar panels, in other embodiments.

Claims (19)

- 8 CLAIMS
1. A spore trap comprising: a rotatable sampling chamber connected to a wind vane, the sampling chamber including a fan unit for the intake of external air samples through an 5 orifice nozzle; a rotatable, electric motor driven sample collector housed within the sampling chamber, adapted to receive and collect spore intake through the orifice nozzle over a user selectable time period; wherein the sample collector is a rotatable disc disposed in a plane substantially orthogonal to the direction of incoming external air.
10
2. A spore trap comprising: a rotatable sampling chamber connected to a wind vane, the sampling chamber including a fan unit for the intake of external air samples through an orifice nozzle; a rotatable, electric motor driven sample collector housed within the sampling chamber, adapted to receive and collect spore intake through the orifice nozzle over a user selectable time period; wherein the fan unit is integral to the sampling chamber 15 and is disposed therein so as to freely rotate with the sampling chamber in response to the operation of the wind vane.
3. A spore trap as claimed in Claim 2, wherein the sample collector is a rotatable disc
disposed in a plane orthogonal to the direction of incoming external air.
4. A spore trap as claimed in Claim 1 or 3, wherein the rotatable disc is transparent.
5. A spore trap as claimed in Claim 1, 3 or 4, wherein the rotatable disc has a diameter of 12cm.
6. A spore trap as claimed in Claim 1, 3, 4 or 5, wherein the rotatable disc includes radial graduations.
7. A spore trap as claimed in any preceding claim, wherein the interconnection 30 between the sample chamber and the wind vane is telescopic.
t - 9 -
8. A spore trap as claimed in any preceding claim, wherein the fan unit and the electric motor require substantially no more than 12V (DC) to operate.
9. A spore trap as claimed in any preceding claim, wherein the spore trap includes 5 control means for controlling the fan unit and the electric motor.
10. A spore trap as claimed in Claim 9, wherein the control means is an electronic control means.
10
11. A spore trap as claimed in Claim 1O, wherein the electronic control means includes an automatic shut-down function for halting further spore sampling by stopping the motor and the fan unit at a desired time position.
12. A spore trap as claimed in any preceding Claim, wherein a tripod for mounting the 15 spore trap includes telescopic legs.
13. A spore trap as claimed in any preceding Claim, wherein the spore trap includes battery supply means.
20
14. A spore trap as claimed in any preceding claim, which is tripodmountable.
15. An array of spore traps, each spore trap comprising: a rotatable sampling chamber connected to a wind vane, the sampling chamber including a fan unit for the intake of external air samples through an orifice nozzle; a rotatable, electric motor driven sample 25 collector housed within the sampling chamber, adapted to receive and collect spore intake through the orifice nozzle over a user selectable time period; wherein the fan unit is integral to the sampling chamber and is disposed therein so as to freely rotate with the sampling chamber in response to the operation of the wind vane; and wherein the array of spore traps are powered by solar panel means.
- 10
16. The array of spore traps, as claimed in Claim 14, wherein a battery supply means is connected to each of the spore traps.
17. The array of spore traps, as claimed in Claim IS, wherein all the battery supply S means are connected to a single solar panel, or an array of solar panels.
18. A spore trap as hereinbefore described, with reference to, and as illustrated by the accompanying Figures 1 to 5.
10
19. An array of spore traps as hereinbefore described, with reference to, and as illustrated by the accompanying Figures 1 to 5.
GB0209148A 2002-04-23 2002-04-23 Spore trap Withdrawn GB2387902A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB0209148A GB2387902A (en) 2002-04-23 2002-04-23 Spore trap

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB0209148A GB2387902A (en) 2002-04-23 2002-04-23 Spore trap

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GB0209148D0 GB0209148D0 (en) 2002-05-29
GB2387902A true GB2387902A (en) 2003-10-29

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Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101806676A (en) * 2010-03-23 2010-08-18 中国科学院研究生院 Pollen collector
CN102183385A (en) * 2011-03-02 2011-09-14 常州浩讯信息科技有限公司 Environmental real-time monitoring and gas sampling method
CN102221488A (en) * 2011-05-24 2011-10-19 北京市劳动保护科学研究所 Constant flow gas sampling device with solid absorbent tubes and method
GB2496995A (en) * 2011-11-28 2013-05-29 Rothamsted Res Ltd Virtual impactor and apparatus for detecting airborne biological particles.
WO2014053236A1 (en) * 2012-10-05 2014-04-10 Sehlinger Torsten Air particle-detection arrangement and system and method for detecting air particles in order to determine the local particle load in the air
GB2510501A (en) * 2011-11-28 2014-08-06 Rothamsted Res Ltd Air sampling device
CN104880337A (en) * 2015-05-28 2015-09-02 国网山西省电力公司电力科学研究院 Vertical gun feeding support convenient and quick to mount and demount
CN107941577A (en) * 2017-11-09 2018-04-20 吉林省农业科学院 A kind of fungal diseases of plants trap setting
CN110692442A (en) * 2019-10-12 2020-01-17 杨桂连 Ganoderma lucidum spore production and collection device and method thereof
WO2020227807A1 (en) * 2019-05-15 2020-11-19 Spornado Inc. Systems and methods for collecting bioaerosols.
EP3754323A1 (en) * 2019-06-18 2020-12-23 Plair SA Automatic spore trap
WO2022000063A1 (en) * 2020-06-30 2022-01-06 UPL Corporation Limited An apparatus for collecting fungal spores from atmosphere, a method thereof and a disease warning system
WO2022232510A1 (en) * 2021-04-30 2022-11-03 Root Applied Sciences Inc. Device for collecting material from air
WO2023284084A1 (en) * 2021-07-14 2023-01-19 海南青峰生物科技有限公司 Intelligent spore capturing and recognizing system device and method based on image processing

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2144850A (en) * 1983-08-12 1985-03-13 Burkard Manufacturing Company Air samplers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2144850A (en) * 1983-08-12 1985-03-13 Burkard Manufacturing Company Air samplers

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WPI Accession No. 1989-014246 & SU 1406152 A *

Cited By (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101806676A (en) * 2010-03-23 2010-08-18 中国科学院研究生院 Pollen collector
CN101806676B (en) * 2010-03-23 2011-11-02 中国科学院研究生院 Pollen collector
CN102183385A (en) * 2011-03-02 2011-09-14 常州浩讯信息科技有限公司 Environmental real-time monitoring and gas sampling method
CN102183385B (en) * 2011-03-02 2012-12-19 常州浩讯信息科技有限公司 Environmental real-time monitoring and gas sampling method
CN102221488A (en) * 2011-05-24 2011-10-19 北京市劳动保护科学研究所 Constant flow gas sampling device with solid absorbent tubes and method
GB2510501B (en) * 2011-11-28 2015-09-09 Rothamsted Res Ltd Air Sampling Device
GB2496995B (en) * 2011-11-28 2014-04-02 Rothamsted Res Ltd Air sampling device
GB2510501A (en) * 2011-11-28 2014-08-06 Rothamsted Res Ltd Air sampling device
GB2496995A (en) * 2011-11-28 2013-05-29 Rothamsted Res Ltd Virtual impactor and apparatus for detecting airborne biological particles.
WO2014053236A1 (en) * 2012-10-05 2014-04-10 Sehlinger Torsten Air particle-detection arrangement and system and method for detecting air particles in order to determine the local particle load in the air
CN104880337A (en) * 2015-05-28 2015-09-02 国网山西省电力公司电力科学研究院 Vertical gun feeding support convenient and quick to mount and demount
CN107941577A (en) * 2017-11-09 2018-04-20 吉林省农业科学院 A kind of fungal diseases of plants trap setting
EP3969875A4 (en) * 2019-05-15 2023-01-25 Spornado Inc. Systems and methods for collecting bioaerosols
WO2020227807A1 (en) * 2019-05-15 2020-11-19 Spornado Inc. Systems and methods for collecting bioaerosols.
EP3754323A1 (en) * 2019-06-18 2020-12-23 Plair SA Automatic spore trap
WO2020254060A1 (en) * 2019-06-18 2020-12-24 Plair Sa Automatic spore trap
CN114026214A (en) * 2019-06-18 2022-02-08 普莱尔股份公司 Automatic spore catcher
CN110692442B (en) * 2019-10-12 2021-07-09 菲朗农业科技(湖北)有限公司 Ganoderma lucidum spore production and collection device
CN110692442A (en) * 2019-10-12 2020-01-17 杨桂连 Ganoderma lucidum spore production and collection device and method thereof
WO2022000063A1 (en) * 2020-06-30 2022-01-06 UPL Corporation Limited An apparatus for collecting fungal spores from atmosphere, a method thereof and a disease warning system
WO2022232510A1 (en) * 2021-04-30 2022-11-03 Root Applied Sciences Inc. Device for collecting material from air
WO2023284084A1 (en) * 2021-07-14 2023-01-19 海南青峰生物科技有限公司 Intelligent spore capturing and recognizing system device and method based on image processing

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