GB212238A - Improved manufacture of anti-diabetic products - Google Patents

Abstract

212,238. 'University of Toronto, Governors of the, (Assignees of Walden, G. B.). March 2, 1923, [Convention date]. Hypodermic injections.-A solution of the anti-diabetic hormone obtained in known manner from the pancreas is adjusted, by the addition of an alkali such as sodium hydroxide or an acid such as hydrochloric so as to have a hydrogen ion contentration within a Ph range of 4 to 7, preferably 4-5 to 5-5, so that a relatively pure precipitate of the active substance is obtained at the isoelectric point. The formation of the precipitate may be accelerated by diluting the solution whilst maintaining the hydrogen ion concentration, and by cooling the solution. The precipitate obtained is dissolved in water containing an acid or a base and adjusted to a Ph value less than 4 or more than 7. The original solution to be treated is preferably the known product obtained by extracting the fresh glands with a solvent such as alcohol which prevents enzyme action, increasing the concentration of the alcohol or other solvent so as to precipitate impurities, further increasing the concentration to precipitate the, active substance, and finally dissolving the active substance so obtained in water. Less pure intermediate products may, however, be employed. The Specification as open to inspection under Sect. 91 (3) (a) refers also to the use of the pancreas and related glands of fishes as a source of the hormone,. This subject-matter does not appear in the Specification as accepted.