GB1507810A - Measurement of alcohol levels in body fluids - Google Patents

Measurement of alcohol levels in body fluids

Info

Publication number
GB1507810A
GB1507810A GB1070/77A GB107077A GB1507810A GB 1507810 A GB1507810 A GB 1507810A GB 1070/77 A GB1070/77 A GB 1070/77A GB 107077 A GB107077 A GB 107077A GB 1507810 A GB1507810 A GB 1507810A
Authority
GB
United Kingdom
Prior art keywords
body fluid
ethanol
oxygen
jan
buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1070/77A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chembro Holdings Pty Ltd
Original Assignee
Chembro Holdings Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chembro Holdings Pty Ltd filed Critical Chembro Holdings Pty Ltd
Publication of GB1507810A publication Critical patent/GB1507810A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1507810 Determination of ethanol in body fluid CHEMBRO HOLDINGS (PTY) Ltd 12 Jan 1977 [15 Jan 1976] 01070/77 Heading G1B Ethanol in a body fluid is determined by a method comprising providing a predetermined volume of body fluid, oxidizing ethanol in the body fluid using an alcohol oxidase in buffer solution in the presence of excess molecular oxygen and measuring the rate of oxygen consumption using for example an oxygen electrode, the oxidation taking place in the presence of an agent operative to suppress the formation of free oxygen by decomosition of peroxide. The agent may be a peroxidase together with o-tolidine, o-dianisidine, aminoantipyrine, or alternatively a catalase inhibitor such as sodium azide. The alcohol oxidase may be obtained from Kloeckera yeast or from a Basidiomycete. The buffer may be a 0À01 to 2 m potassum phosphate buffer pH 7 to 9, and the reaction mixture may contain 10 microns to 10 mm of a metal ion complexing substance such as EDTA-Na.
GB1070/77A 1976-01-15 1977-01-12 Measurement of alcohol levels in body fluids Expired GB1507810A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ZA760233A ZA76233B (en) 1976-01-15 1976-01-15 Measurement of alcohol levels in body fluids

Publications (1)

Publication Number Publication Date
GB1507810A true GB1507810A (en) 1978-04-19

Family

ID=25569865

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1070/77A Expired GB1507810A (en) 1976-01-15 1977-01-12 Measurement of alcohol levels in body fluids

Country Status (11)

Country Link
JP (1) JPS5288394A (en)
AU (1) AU505647B2 (en)
BE (1) BE850383A (en)
CA (1) CA1085279A (en)
DE (1) DE2701168A1 (en)
FR (1) FR2338495A1 (en)
GB (1) GB1507810A (en)
IE (1) IE44476B1 (en)
NL (1) NL7700340A (en)
SE (1) SE7700261L (en)
ZA (1) ZA76233B (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0019937A1 (en) * 1979-06-05 1980-12-10 Phillips Petroleum Company A method for producing alcohol oxidase, alcohol oxidase, enzyme preparation and a method for determining the concentration of a compound in an alcohol-containing sample
EP0117032A1 (en) * 1983-01-12 1984-08-29 Alcoholism And Drug Addiction Research Foundation Rapid analysis of ethanol in body fluids
EP0133481A1 (en) * 1983-07-12 1985-02-27 Lifescan, Inc. Colorimetric ethanol analysis method and test device
US4540668A (en) * 1979-06-05 1985-09-10 Phillips Petroleum Company Alcohol oxidase from Pichia-type yeasts
US4556635A (en) * 1983-08-29 1985-12-03 Phillips Petroleum Company Determination of alcohol content in water imiscible organic systems
US5264106A (en) * 1988-10-07 1993-11-23 Medisense, Inc. Enhanced amperometric sensor

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4401122A (en) * 1979-08-02 1983-08-30 Children's Hospital Medical Center Cutaneous methods of measuring body substances
JPS59232097A (en) * 1983-05-16 1984-12-26 Shokuhin Sangyo Center Method and apparatus for determination of freshness
EP0214336A1 (en) * 1985-09-13 1987-03-18 Phillips Petroleum Company Determination of alcohol content in water immiscible organic systems
AT390803B (en) * 1988-08-24 1990-07-10 Avl Verbrennungskraft Messtech METHOD FOR DETERMINING THE CONCENTRATION OF AN ENZYME SUBSTRATE AND SENSOR FOR IMPLEMENTING THE METHOD
AU625439B2 (en) * 1988-10-07 1992-07-09 Medisense Inc. Enhanced amperometric sensor
DE3934299C1 (en) * 1989-10-13 1990-10-25 Gesellschaft Fuer Biotechnologische Forschung Mbh (Gbf), 3300 Braunschweig, De

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1351547A (en) * 1971-03-22 1974-05-01 Wothington Biochemical Corp Determination of the ethanol content of a material

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0019937A1 (en) * 1979-06-05 1980-12-10 Phillips Petroleum Company A method for producing alcohol oxidase, alcohol oxidase, enzyme preparation and a method for determining the concentration of a compound in an alcohol-containing sample
US4540668A (en) * 1979-06-05 1985-09-10 Phillips Petroleum Company Alcohol oxidase from Pichia-type yeasts
EP0117032A1 (en) * 1983-01-12 1984-08-29 Alcoholism And Drug Addiction Research Foundation Rapid analysis of ethanol in body fluids
EP0133481A1 (en) * 1983-07-12 1985-02-27 Lifescan, Inc. Colorimetric ethanol analysis method and test device
AU586150B2 (en) * 1983-07-12 1989-07-06 Lifescan, Inc. Colorimetric ethanol analysis method and test device
US4556635A (en) * 1983-08-29 1985-12-03 Phillips Petroleum Company Determination of alcohol content in water imiscible organic systems
US5264106A (en) * 1988-10-07 1993-11-23 Medisense, Inc. Enhanced amperometric sensor

Also Published As

Publication number Publication date
BE850383A (en) 1977-07-14
DE2701168A1 (en) 1977-07-21
IE44476B1 (en) 1981-12-16
IE44476L (en) 1977-07-15
CA1085279A (en) 1980-09-09
JPS5288394A (en) 1977-07-23
NL7700340A (en) 1977-07-19
AU505647B2 (en) 1979-11-29
ZA76233B (en) 1977-08-31
SE7700261L (en) 1977-07-16
AU2126177A (en) 1978-07-20
FR2338495A1 (en) 1977-08-12

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee