IE44476L - Measuring ethanol in a body fluid - Google Patents

Measuring ethanol in a body fluid

Info

Publication number
IE44476L
IE44476L IE770048A IE4877A IE44476L IE 44476 L IE44476 L IE 44476L IE 770048 A IE770048 A IE 770048A IE 4877 A IE4877 A IE 4877A IE 44476 L IE44476 L IE 44476L
Authority
IE
Ireland
Prior art keywords
body fluid
ethanol
oxygen
jan
buffer
Prior art date
Application number
IE770048A
Other versions
IE44476B1 (en
Original Assignee
Chembro Holdings Propietary Lt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chembro Holdings Propietary Lt filed Critical Chembro Holdings Propietary Lt
Publication of IE44476L publication Critical patent/IE44476L/en
Publication of IE44476B1 publication Critical patent/IE44476B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1507810 Determination of ethanol in body fluid CHEMBRO HOLDINGS (PTY) Ltd 12 Jan 1977 [15 Jan 1976] 01070/77 Heading G1B Ethanol in a body fluid is determined by a method comprising providing a predetermined volume of body fluid, oxidizing ethanol in the body fluid using an alcohol oxidase in buffer solution in the presence of excess molecular oxygen and measuring the rate of oxygen consumption using for example an oxygen electrode, the oxidation taking place in the presence of an agent operative to suppress the formation of free oxygen by decomosition of peroxide. The agent may be a peroxidase together with o-tolidine, o-dianisidine, aminoantipyrine, or alternatively a catalase inhibitor such as sodium azide. The alcohol oxidase may be obtained from Kloeckera yeast or from a Basidiomycete. The buffer may be a 0À01 to 2 m potassum phosphate buffer pH 7 to 9, and the reaction mixture may contain 10 microns to 10 mm of a metal ion complexing substance such as EDTA-Na. [GB1507810A]
IE48/77A 1976-01-15 1977-01-11 Measurement of alcohol levels in body fluids IE44476B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ZA760233A ZA76233B (en) 1976-01-15 1976-01-15 Measurement of alcohol levels in body fluids

Publications (2)

Publication Number Publication Date
IE44476L true IE44476L (en) 1977-07-15
IE44476B1 IE44476B1 (en) 1981-12-16

Family

ID=25569865

Family Applications (1)

Application Number Title Priority Date Filing Date
IE48/77A IE44476B1 (en) 1976-01-15 1977-01-11 Measurement of alcohol levels in body fluids

Country Status (11)

Country Link
JP (1) JPS5288394A (en)
AU (1) AU505647B2 (en)
BE (1) BE850383A (en)
CA (1) CA1085279A (en)
DE (1) DE2701168A1 (en)
FR (1) FR2338495A1 (en)
GB (1) GB1507810A (en)
IE (1) IE44476B1 (en)
NL (1) NL7700340A (en)
SE (1) SE7700261L (en)
ZA (1) ZA76233B (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1157399A (en) * 1979-06-05 1983-11-22 Thomas R. Hopkins Alcohol oxidase from pichia-type yeasts
US4540668A (en) * 1979-06-05 1985-09-10 Phillips Petroleum Company Alcohol oxidase from Pichia-type yeasts
US4401122A (en) * 1979-08-02 1983-08-30 Children's Hospital Medical Center Cutaneous methods of measuring body substances
DE3477812D1 (en) * 1983-01-12 1989-05-24 Alcoholism & Drug Addiction Rapid analysis of ethanol in body fluids
JPS59232097A (en) * 1983-05-16 1984-12-26 Shokuhin Sangyo Center Method and apparatus for determination of freshness
US4734360A (en) * 1983-07-12 1988-03-29 Lifescan, Inc. Colorimetric ethanol analysis method and test device
US4556635A (en) * 1983-08-29 1985-12-03 Phillips Petroleum Company Determination of alcohol content in water imiscible organic systems
EP0214336A1 (en) * 1985-09-13 1987-03-18 Phillips Petroleum Company Determination of alcohol content in water immiscible organic systems
AT390803B (en) * 1988-08-24 1990-07-10 Avl Verbrennungskraft Messtech METHOD FOR DETERMINING THE CONCENTRATION OF AN ENZYME SUBSTRATE AND SENSOR FOR IMPLEMENTING THE METHOD
US5264106A (en) * 1988-10-07 1993-11-23 Medisense, Inc. Enhanced amperometric sensor
DE68925104T2 (en) * 1988-10-07 1996-05-09 Medisense Inc Amperometric sensor
DE3934299C1 (en) * 1989-10-13 1990-10-25 Gesellschaft Fuer Biotechnologische Forschung Mbh (Gbf), 3300 Braunschweig, De

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1351547A (en) * 1971-03-22 1974-05-01 Wothington Biochemical Corp Determination of the ethanol content of a material

Also Published As

Publication number Publication date
GB1507810A (en) 1978-04-19
AU505647B2 (en) 1979-11-29
DE2701168A1 (en) 1977-07-21
NL7700340A (en) 1977-07-19
IE44476B1 (en) 1981-12-16
JPS5288394A (en) 1977-07-23
SE7700261L (en) 1977-07-16
AU2126177A (en) 1978-07-20
ZA76233B (en) 1977-08-31
FR2338495A1 (en) 1977-08-12
BE850383A (en) 1977-07-14
CA1085279A (en) 1980-09-09

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