GB1351547A - Determination of the ethanol content of a material - Google Patents

Determination of the ethanol content of a material

Info

Publication number
GB1351547A
GB1351547A GB1058972A GB1058972A GB1351547A GB 1351547 A GB1351547 A GB 1351547A GB 1058972 A GB1058972 A GB 1058972A GB 1058972 A GB1058972 A GB 1058972A GB 1351547 A GB1351547 A GB 1351547A
Authority
GB
United Kingdom
Prior art keywords
solution
ethanol
int
ethanol content
march
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1058972A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
WOTHINGTON BIOCHEMICAL CORP
Original Assignee
WOTHINGTON BIOCHEMICAL CORP
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by WOTHINGTON BIOCHEMICAL CORP filed Critical WOTHINGTON BIOCHEMICAL CORP
Publication of GB1351547A publication Critical patent/GB1351547A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1351547 Enzymatic determination of ethanol WORTHINGTON BIOCHEMICAL CORP 7 March 1972 [22 March 1971] 10589/72 Heading G1B In a method for the enzymatic determination of the ethanol content of an ethanol-containing material, a sample of ethanol-containing material is commingled with an aqueous solution of alcohol dehydrogenase that contains 2-piodophenyl-3-p-nitrophenyl-5-phenyl tetrazolium chloride ("INT") in an amount that in the reaction system is at least sufficient to be quantitatively responsive to the ethanol content of the sample as regards conversion to formazan, nictoinamide-adenine-nucliotide, diaphorase and a buffer which in said solution is substantially inert and which maintains the pH of the solution at a value from 7À5 to 9À5 under testing conditions, The solution may also contain albumin or gelatine as stabilizer and glutathione as antioxidant. A test composition may comprise a lyophilized mixture of the components other than INT, a solution of INT being separately lyophilized. The process is applicable to wine, vinegar or biological fluids e.g. serum.
GB1058972A 1971-03-22 1972-03-07 Determination of the ethanol content of a material Expired GB1351547A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US12705471A 1971-03-22 1971-03-22

Publications (1)

Publication Number Publication Date
GB1351547A true GB1351547A (en) 1974-05-01

Family

ID=22428091

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1058972A Expired GB1351547A (en) 1971-03-22 1972-03-07 Determination of the ethanol content of a material

Country Status (7)

Country Link
AU (1) AU461239B2 (en)
CA (1) CA980667A (en)
DE (1) DE2213729A1 (en)
ES (1) ES401014A1 (en)
FR (1) FR2131504A5 (en)
GB (1) GB1351547A (en)
IT (1) IT972326B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0117032A1 (en) * 1983-01-12 1984-08-29 Alcoholism And Drug Addiction Research Foundation Rapid analysis of ethanol in body fluids
US5916746A (en) * 1996-05-09 1999-06-29 Kirkegaard & Perry Laboratories, Inc. Formazan-based immunoassay

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2334955A1 (en) * 1975-10-15 1977-07-08 Calbiochem Enzymatic ethanol assay in body fluids - by using amino-propane-1,3-diol as buffer and trapping agent
ZA76233B (en) * 1976-01-15 1977-08-31 Chembro Holdings Pty Ltd Measurement of alcohol levels in body fluids
EP0154409A3 (en) * 1984-02-06 1988-03-02 Unitika Ltd. Testing material for detecting alcohols

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0117032A1 (en) * 1983-01-12 1984-08-29 Alcoholism And Drug Addiction Research Foundation Rapid analysis of ethanol in body fluids
US5916746A (en) * 1996-05-09 1999-06-29 Kirkegaard & Perry Laboratories, Inc. Formazan-based immunoassay

Also Published As

Publication number Publication date
ES401014A1 (en) 1975-10-16
FR2131504A5 (en) 1972-11-10
AU461239B2 (en) 1975-05-05
IT972326B (en) 1974-05-20
DE2213729A1 (en) 1972-09-28
CA980667A (en) 1975-12-30
AU3990072A (en) 1973-03-13

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee