GB1494742A - Process for recovering single cell micro-organisms from fermentation broth - Google Patents

Process for recovering single cell micro-organisms from fermentation broth

Info

Publication number
GB1494742A
GB1494742A GB1210174A GB1210174A GB1494742A GB 1494742 A GB1494742 A GB 1494742A GB 1210174 A GB1210174 A GB 1210174A GB 1210174 A GB1210174 A GB 1210174A GB 1494742 A GB1494742 A GB 1494742A
Authority
GB
United Kingdom
Prior art keywords
cream
range
yeast
broth
organisms
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1210174A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BP PLC
Original Assignee
BP PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BP PLC filed Critical BP PLC
Priority to GB1210174A priority Critical patent/GB1494742A/en
Priority to IT4852775A priority patent/IT1030000B/en
Priority to JP3102275A priority patent/JPS50132172A/ja
Priority to DE19752511436 priority patent/DE2511436C3/en
Priority to FR7508420A priority patent/FR2264870B1/fr
Priority to SU752117934A priority patent/SU611594A3/en
Publication of GB1494742A publication Critical patent/GB1494742A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/26—Processes using, or culture media containing, hydrocarbons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Fats And Perfumes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

1494742 Yeast &c BRITISH PETROLEUM CO Ltd 27 Feb 1975 [19 March 1974] 12101/74 Heading C6F An improved process for separating a cream containing single cell micro-organisms (yeast or bacteria) from a fermentation broth involves centrifuging the broth (or a fraction thereof) containing single cell micro-organisms to give a cream, heating the cream to increase its temperature by a value in the range 5‹ to 70‹C and centrifuging the heated cream to remove water and give a cream having an increased solids content. The temperature is increased typically by a value in the range 40‹ to 45‹C in the case of a cream obtained by centrifuging a broth produced by cultivating a hydrocarbon utilizing yeast using a gas oil hydrocarbon boiling in the range 300‹-380‹C as the carbon source, and by 15‹-50‹C in the case of a broth and cream from the cultivation of yeast on normal paraffins recovered from a petroleum fraction in the kerosine or gas oil boiling ranges. The cream may be heated by steam injection or in a plate heat exchanger for, say, 0À1-5 minutes after any necessary adjustment of pH to a value in the range 3À0-9À0. The cell temperature produced will be of the order 50‹- 80‹C. The heated cream is then centrifuged in a nozzle type or decanter type centrifuge. Examples are directed to the separation of a cream from broths obtained by cultivating Candida lipolytica on a petroleum fraction consisting of a mixture of C 11 to C 24 n-paraffins, and Candida tropicalis on a petroleum fraction consisting of a mixture of gas oil hydrocarbons with a normal paraffins content (C 14 to C 28 ) in the range 11 to 18% respectively. Creams having a substantially increased solids content of the order 22-26 wt per cent were obtained by the heating and centrifugation according to the invention.
GB1210174A 1974-03-19 1974-03-19 Process for recovering single cell micro-organisms from fermentation broth Expired GB1494742A (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
GB1210174A GB1494742A (en) 1974-03-19 1974-03-19 Process for recovering single cell micro-organisms from fermentation broth
IT4852775A IT1030000B (en) 1974-03-19 1975-03-10 PROCESS TO RECOVER MICROORGANISMS FROM A FERMENTATION BROTH
JP3102275A JPS50132172A (en) 1974-03-19 1975-03-14
DE19752511436 DE2511436C3 (en) 1974-03-19 1975-03-15 Obtaining a cream containing unicellular microorganisms from a fermentation broth
FR7508420A FR2264870B1 (en) 1974-03-19 1975-03-18
SU752117934A SU611594A3 (en) 1974-03-19 1975-03-19 Method of isolating unicellular microorganisms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1210174A GB1494742A (en) 1974-03-19 1974-03-19 Process for recovering single cell micro-organisms from fermentation broth

Publications (1)

Publication Number Publication Date
GB1494742A true GB1494742A (en) 1977-12-14

Family

ID=9998407

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1210174A Expired GB1494742A (en) 1974-03-19 1974-03-19 Process for recovering single cell micro-organisms from fermentation broth

Country Status (6)

Country Link
JP (1) JPS50132172A (en)
DE (1) DE2511436C3 (en)
FR (1) FR2264870B1 (en)
GB (1) GB1494742A (en)
IT (1) IT1030000B (en)
SU (1) SU611594A3 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD124534A1 (en) * 1976-03-01 1977-03-02
DE2805823C3 (en) * 1978-02-11 1980-08-28 Gesellschaft Fuer Biotechnologische Forschung Mbh (Gbf), 3300 Braunschweig Processes and systems for flooding petroleum deposits and oil sands

Also Published As

Publication number Publication date
JPS50132172A (en) 1975-10-20
IT1030000B (en) 1979-03-20
DE2511436A1 (en) 1975-09-25
SU611594A3 (en) 1978-06-15
DE2511436B2 (en) 1977-10-20
DE2511436C3 (en) 1978-06-08
FR2264870B1 (en) 1978-12-08
FR2264870A1 (en) 1975-10-17

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee