GB1208572A - Improvements in or relating to the cultivation of micro-organisms - Google Patents

Improvements in or relating to the cultivation of micro-organisms

Info

Publication number
GB1208572A
GB1208572A GB1082767A GB1082767A GB1208572A GB 1208572 A GB1208572 A GB 1208572A GB 1082767 A GB1082767 A GB 1082767A GB 1082767 A GB1082767 A GB 1082767A GB 1208572 A GB1208572 A GB 1208572A
Authority
GB
United Kingdom
Prior art keywords
yeast
minutes
heat treatment
cells
proportion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1082767A
Inventor
Ky Charles Ninh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BP PLC
Original Assignee
BP PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BP PLC filed Critical BP PLC
Priority to GB1082767A priority Critical patent/GB1208572A/en
Priority to NL6802848A priority patent/NL6802848A/xx
Priority to DE19681642601 priority patent/DE1642601A1/en
Priority to FR1560717D priority patent/FR1560717A/fr
Priority to BE711916D priority patent/BE711916A/xx
Publication of GB1208572A publication Critical patent/GB1208572A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

1,208,572. Recovering yeast &c. BRITISH PETROLEUM CO. Ltd. Feb. 8, 1968 [March 8, 1967], No. 10827/67. Heading C6F. A material consisting of, or containing, a major proportion by weight of a micro-organism and a minor proportion of a hydrocarbon is heated under conditions such that the cell walls of the cells of the micro-organism undergo change but such that after the heat treatment a major proportion of the cells remain intact cells i.e. cells which retain their cytoplasm within their cell walls. Examples and comparative experiments demonstrate that the phosphorus and ash contents of the yeast product are lower when the heat treatment is included. According to an example, the product stream from the cultivation of Candida tropicalis on a gas oil in the presence of an aqueous nutrient medium and a free oxygen-containing gas, was decanted to remove aqueous phase. Fresh water was added to the yeast cream in order to reconstitute the original mixture, a small proportion of a lauric alcohol oxyethylenated and sulphated detergent was added to the mixture and stirred, and the emulsion produced was centrifuged to give a gas oil phase, an aqueous phase and a yeast paste. The yeast paste, diluted with water, was heat treated at a temperature of 100‹ C. for one hour in a vessel equipped with a propeller and then centrifuged hot. The yeast slurry thus obtained was dried to a cream in a drum drier, and the cream (containing 50% by weight of yeast) was twice extracted with a solvent mixture consisting of equal parts by weight of n-hexane and isopropanol firstly at 80‹ C. for 30 minutes and then at 50‹ C. for 30 minutes. After filtration of the solvent loaded with some yeast impurities, the remaining product was treated with isopropanol at 50‹ C. for 10 minutes. Finally, the yeast product was dried with superheated steam and the moisture, lipid, phosphorus, ash and nitrogen contents of the yeast were determined. The proportion of intact cells in the material after the heat treatment was 95%. In further examples the heat treatment consisted of: (a) injecting steam at 50 psig to hold the temperature of the yeast at 100‹ C. for 30 minutes; (b) washing for 2 hours with hot water to maintain the yeast at 90‹ C.; or (c) heating at 70-80‹ C. for 30 minutes.
GB1082767A 1967-03-08 1967-03-08 Improvements in or relating to the cultivation of micro-organisms Expired GB1208572A (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
GB1082767A GB1208572A (en) 1967-03-08 1967-03-08 Improvements in or relating to the cultivation of micro-organisms
NL6802848A NL6802848A (en) 1967-03-08 1968-02-29
DE19681642601 DE1642601A1 (en) 1967-03-08 1968-03-07 Process for obtaining a microorganism or a fraction containing a microorganism
FR1560717D FR1560717A (en) 1967-03-08 1968-03-07
BE711916D BE711916A (en) 1967-03-08 1968-03-08

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1082767A GB1208572A (en) 1967-03-08 1967-03-08 Improvements in or relating to the cultivation of micro-organisms

Publications (1)

Publication Number Publication Date
GB1208572A true GB1208572A (en) 1970-10-14

Family

ID=9975036

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1082767A Expired GB1208572A (en) 1967-03-08 1967-03-08 Improvements in or relating to the cultivation of micro-organisms

Country Status (5)

Country Link
BE (1) BE711916A (en)
DE (1) DE1642601A1 (en)
FR (1) FR1560717A (en)
GB (1) GB1208572A (en)
NL (1) NL6802848A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2154091A1 (en) * 1970-10-30 1972-05-04 Standard Oil Co , Chicago, 111 (V St A) Unicellular protein materials from ethanol

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0632603B2 (en) * 1983-12-26 1994-05-02 中外製薬株式会社 Removal of off-flavor components attached to bacterial cells

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2154091A1 (en) * 1970-10-30 1972-05-04 Standard Oil Co , Chicago, 111 (V St A) Unicellular protein materials from ethanol

Also Published As

Publication number Publication date
NL6802848A (en) 1968-09-09
DE1642601A1 (en) 1971-05-06
BE711916A (en) 1968-09-09
FR1560717A (en) 1969-03-21

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee