GB1479661A - Method and apparatus for detection and purification of proteins and antibodies - Google Patents

Method and apparatus for detection and purification of proteins and antibodies

Info

Publication number
GB1479661A
GB1479661A GB2468874A GB2468874A GB1479661A GB 1479661 A GB1479661 A GB 1479661A GB 2468874 A GB2468874 A GB 2468874A GB 2468874 A GB2468874 A GB 2468874A GB 1479661 A GB1479661 A GB 1479661A
Authority
GB
United Kingdom
Prior art keywords
protein
wafer
surface
solution
substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2468874A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Electric Co
Original Assignee
General Electric Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US26627872A priority Critical
Priority to US38411373A priority
Application filed by General Electric Co filed Critical General Electric Co
Publication of GB1479661A publication Critical patent/GB1479661A/en
Application status is Expired legal-status Critical

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B82NANOTECHNOLOGY
    • B82YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B82NANOTECHNOLOGY
    • B82YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00Nanotechnology for materials or surface science, e.g. nanocomposites
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B82NANOTECHNOLOGY
    • B82YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • G01N33/54373Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553Metal or metal coated

Abstract

1479661 Detection of protein; slide device GENERAL ELECTRIC CO 4 June 1974 [30 July 1973] 24688/74 Addition to 1443181 Heading G1B A method of testing a solution for the presence in it of a protein comprises depositing by evaporation on to a wafer of a light transmissive substrate material a metal having high atomic mobility and poor surface wetting characteristics, immersing the wafer in a solution of a protein specifically reactive with the protein to be tested for to coat the wafer with a monomolecular layer of the specifically reactive protein, immersing the coated wafer in the solution to be tested, removing the wafer from the solution, placing it with the protein-coated surface in contact with a surface of a light-reflecting liquid such as mercury and examining the wafer to determine whether it has on it a bimolecular or a monomolecular protein layer. A device used in detecting protein in a liquid comprises a substrate having a gold surface, a layer of another metal such as indium between the gold surface and the substrate and attached to the gold surface a monomolecular layer of a protein specifically reactive with the protein to be detected. The substrate may be a glass slide. The other metal may be indium as mentioned, or gold, silver, tin or lead. The method and device described may be used in direct or indirect testing. Diagnosis of hepatitis is described, and there is a reference to pregnancy testing.
GB2468874A 1972-06-26 1974-06-04 Method and apparatus for detection and purification of proteins and antibodies Expired GB1479661A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US26627872A true 1972-06-26 1972-06-26
US38411373A true 1973-07-30 1973-07-30

Publications (1)

Publication Number Publication Date
GB1479661A true GB1479661A (en) 1977-07-13

Family

ID=26951735

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2468874A Expired GB1479661A (en) 1972-06-26 1974-06-04 Method and apparatus for detection and purification of proteins and antibodies

Country Status (2)

Country Link
FR (1) FR2301824B2 (en)
GB (1) GB1479661A (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE431257B (en) * 1977-11-03 1984-01-23 Du Pont Seen and device for immunoassay magnetic metal or metal oxide merksubstans
EP0067921B1 (en) * 1981-06-22 1987-11-11 Prutec Limited A method for determining bioactive substances

Also Published As

Publication number Publication date
FR2301824A2 (en) 1976-09-17
FR2301824B2 (en) 1980-04-11

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Legal Events

Date Code Title Description
PS Patent sealed