GB1443181A - Method and apparatus for detection and purification of proteins and antibodies - Google Patents

Method and apparatus for detection and purification of proteins and antibodies

Info

Publication number
GB1443181A
GB1443181A GB3018273A GB3018273A GB1443181A GB 1443181 A GB1443181 A GB 1443181A GB 3018273 A GB3018273 A GB 3018273A GB 3018273 A GB3018273 A GB 3018273A GB 1443181 A GB1443181 A GB 1443181A
Authority
GB
United Kingdom
Prior art keywords
substrate
protein
separated
metal
layer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3018273A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Electric Co
Original Assignee
General Electric Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Electric Co filed Critical General Electric Co
Priority to GB892276A priority Critical patent/GB1443182A/en
Publication of GB1443181A publication Critical patent/GB1443181A/en
Priority to GB3509776A priority patent/GB1571988A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/961—Chemistry: molecular biology and microbiology including a step of forming, releasing, or exposing the antigen or forming the hapten-immunogenic carrier complex or the antigen per se
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/806—Antigenic peptides or proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/826—Viruses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/26—Web or sheet containing structurally defined element or component, the element or component having a specified physical dimension
    • Y10T428/263—Coating layer not in excess of 5 mils thick or equivalent
    • Y10T428/264—Up to 3 mils
    • Y10T428/265—1 mil or less

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Nanotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Materials Engineering (AREA)
  • Composite Materials (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Inorganic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medical Informatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

1443181 Separating proteins GENERAL ELECTRIC CO 26 June 1973 [26 June 1973] 30182/73 Heading C3H [Also in Division G1 A protein is separated from a solution containing it by a method comprising immersing a substrate in a solution of a protein specifically reactive with the protein to be separated, so forming a monomolecular layer of the specifically reactive protein on the substrate, then immersing the substrate in the solution containing the protein to be separated removing the substrate therefrom and examining it for formation of a bimolecular layer. The substrate may be of metal, glass, mica, plastics material, fused silica or quartz, and is preferably a glass slide either coated with metal or metal oxide or having on its surface globules of a metal for example indium produced by evaporation. The examination of the slide to detect a bimolecular layer may be carried out by one of the methods shown in the drawing. The substrate may then be immersed in a weak acid to sever the bonds between the proteins, or alternatively the bimolecular layer on the substrate may be brought temporarily into contact with a drop of a weak acid on a second substrate, when the second substrate retains a monomolecular layer of the protein to be separated.
GB3018273A 1972-06-26 1973-06-26 Method and apparatus for detection and purification of proteins and antibodies Expired GB1443181A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GB892276A GB1443182A (en) 1972-06-26 1973-06-26 Method and apparatus for separating proteins and antibodies
GB3509776A GB1571988A (en) 1972-06-26 1976-08-24 Method and apparatus for concentration and purification of antigens and antibodies

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US26627872A 1972-06-26 1972-06-26

Publications (1)

Publication Number Publication Date
GB1443181A true GB1443181A (en) 1976-07-21

Family

ID=23013914

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3018273A Expired GB1443181A (en) 1972-06-26 1973-06-26 Method and apparatus for detection and purification of proteins and antibodies

Country Status (8)

Country Link
US (1) US4172827A (en)
JP (2) JPS5938543B2 (en)
CA (1) CA1025772A (en)
DE (2) DE2330702C2 (en)
FR (1) FR2191744A5 (en)
GB (1) GB1443181A (en)
IT (1) IT990685B (en)
SE (2) SE459455B (en)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1047918A (en) * 1973-07-30 1979-02-06 General Electric Company Method and apparatus for detection and purification of proteins and antibodies
US3904367A (en) * 1973-08-15 1975-09-09 Gen Electric Contrast enhancement for immunological film detection
SE387746B (en) * 1974-05-29 1976-09-13 Pharmacia Diagnostics Ab PROCEDURE FOR VISUAL DISPLAY OF ANTIBODIES IN AN AQUATIC SAMPLE
US3979509A (en) * 1974-09-03 1976-09-07 General Electric Company Opaque layer method for detecting biological particles
US4041146A (en) * 1975-05-01 1977-08-09 General Electric Company Method for detection of biological particles
US3979184A (en) * 1975-05-27 1976-09-07 General Electric Company Diagnostic device for visually detecting presence of biological particles
JPS5238789A (en) * 1975-08-14 1977-03-25 Sinai School Medicine Method of identifying hematocyte type and adaptability
GB1555142A (en) * 1975-08-14 1979-11-07 Sinai School Medicine Blood cell typing and compatibility test procedure
DE2638250C2 (en) * 1975-08-27 1985-11-28 General Electric Co., Schenectady, N.Y. Diagnostic method for determining the presence or absence of a specific protein in a biological sample, as well as device for carrying out the method
DE2638251A1 (en) * 1975-08-27 1977-06-08 Gen Electric Antibody purification - by dipping antigen-coated substrate into antibody solution then dipping into immunological complex splitting reagent
US4090849A (en) * 1976-12-20 1978-05-23 General Electric Company Diagnostic device and manufacture thereof
US4066646A (en) * 1976-12-23 1978-01-03 General Electric Company Diagnostic device and housing therefor
SE7811630L (en) * 1977-11-17 1979-05-18 Welsh Nat School Med METHOD OF DETECTION OR QUANTITATION OF SUBSTANCES USING LABELING TECHNIQUES
US4280815A (en) * 1979-06-18 1981-07-28 Technicon Instruments Corporation Electrochemiluminescent immunoassay and apparatus therefor
US4554101A (en) * 1981-01-09 1985-11-19 New York Blood Center, Inc. Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity
EP0067921B1 (en) * 1981-06-22 1987-11-11 Prutec Limited A method for determining bioactive substances
US4414197A (en) * 1982-03-19 1983-11-08 Ortho Diagnostic Systems Inc. Method for preparing permanent slides of rare sorted cells
US4489133A (en) * 1982-11-16 1984-12-18 The Board Of Trustees Of The Leland Stanford Jr. University Two-dimensional crystallization technique
US4568488A (en) * 1984-01-11 1986-02-04 Lee Huang Sylvia Reverse immunoaffinity chromatography purification method
DE3506703C1 (en) * 1985-02-26 1986-04-30 Sagax Instrument AB, Sundbyberg Process for sequence analysis of nucleic acids, in particular deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), as well as carriers for carrying out the method and process for producing the carrier
US6060237A (en) * 1985-02-26 2000-05-09 Biostar, Inc. Devices and methods for optical detection of nucleic acid hybridization
US4634599A (en) * 1985-05-02 1987-01-06 General Electric Company Method for making ordered monolayers of macromolecules
US4668584A (en) * 1985-12-23 1987-05-26 General Electric Company Method for forming 2-D crystals of proteins and macromolecules
US5468606A (en) * 1989-09-18 1995-11-21 Biostar, Inc. Devices for detection of an analyte based upon light interference
US5482830A (en) * 1986-02-25 1996-01-09 Biostar, Inc. Devices and methods for detection of an analyte based upon light interference
US5089387A (en) * 1988-07-07 1992-02-18 Adeza Biomedical Corporation Dna probe diffraction assay and reagents
CA1337173C (en) * 1989-04-28 1995-10-03 Westaim Biomedical Corp. Thin film diagnostic device
US5639671A (en) * 1989-09-18 1997-06-17 Biostar, Inc. Methods for optimizing of an optical assay device
US5541057A (en) * 1989-09-18 1996-07-30 Biostar, Inc. Methods for detection of an analyte
US5955377A (en) * 1991-02-11 1999-09-21 Biostar, Inc. Methods and kits for the amplification of thin film based assays
US5550063A (en) * 1991-02-11 1996-08-27 Biostar, Inc. Methods for production of an optical assay device
ATE158080T1 (en) * 1991-10-01 1997-09-15 Biostar Inc HIGHLY SENSITIVE OPTICAL IMMUNOASSAY BY USE OF ENZYM LABELED REAGENT
US5418136A (en) * 1991-10-01 1995-05-23 Biostar, Inc. Devices for detection of an analyte based upon light interference
US5494829A (en) * 1992-07-31 1996-02-27 Biostar, Inc. Devices and methods for detection of an analyte based upon light interference
US5552272A (en) * 1993-06-10 1996-09-03 Biostar, Inc. Detection of an analyte by fluorescence using a thin film optical device
US5413939A (en) * 1993-06-29 1995-05-09 First Medical, Inc. Solid-phase binding assay system for interferometrically measuring analytes bound to an active receptor
US5679579A (en) * 1996-01-29 1997-10-21 First Medical, Inc. Immunofluorescence measurement of analytes bound to a substrate and apparatus therefor
DE19629243A1 (en) * 1996-07-19 1998-01-29 Udo Dr Ris Method for the quantitative and / or qualitative determination of atoms or molecules
DE19631413A1 (en) * 1996-08-05 1998-02-12 Stefan Prof Dr Seeger Method and device for preparing molecules and particles
WO2019104438A1 (en) * 2017-12-01 2019-06-06 Valorisation-Recherche, Limited Partnership Coated glass slide for enhanced thin tissue section adhesion
CN109540829A (en) * 2018-12-12 2019-03-29 暨南大学 A kind of infrared spectroscopy detection technique is used for the method for making sample of Crohn disease antidiastole

Family Cites Families (10)

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Publication number Priority date Publication date Assignee Title
US2666355A (en) * 1951-10-12 1954-01-19 Hans J Trurnit Method of studying chemical reactions by measuring interfacial film thicknesses
US3236732A (en) * 1962-01-22 1966-02-22 Edward R Arquilla Pregnancy test method and immunological indicator therefor
US3171783A (en) * 1962-08-15 1965-03-02 Hyland Lab Diagnostic procedure
US3313706A (en) * 1965-06-08 1967-04-11 Johnson & Johnson Diagnostic antigen for viral hepatitis
GB1179435A (en) * 1966-06-17 1970-01-28 Ortho Pharma Corp Stable Reagent for the Detection of Rheumatoid Arthritis
US3492396A (en) * 1967-03-13 1970-01-27 Becton Dickinson Co Agglutinate separation method and apparatus
AT279804B (en) 1967-08-22 1970-03-25 Barbara Dr Polheim Procedure for the isolation of antigens and antibodies
US3639558A (en) * 1968-02-19 1972-02-01 Louis Csizmas Immunological reagent particles having proteinaceous materials covalently bonded thereto
US3646346A (en) * 1968-12-26 1972-02-29 Pharmacia Ab Antibody-coated tube system for radioimmunoassay
US3697645A (en) * 1971-04-28 1972-10-10 Us Army Purification of antibodies

Also Published As

Publication number Publication date
FR2191744A5 (en) 1974-02-01
DE2366615C2 (en) 1986-10-23
DE2330702A1 (en) 1974-01-10
JPS5798216A (en) 1982-06-18
SE7610478L (en) 1976-09-21
SE440657B (en) 1985-08-12
DE2330702C2 (en) 1985-11-28
JPS5938543B2 (en) 1984-09-18
SE459455B (en) 1989-07-03
IT990685B (en) 1975-07-10
US4172827A (en) 1979-10-30
CA1025772A (en) 1978-02-07
JPS628149B2 (en) 1987-02-20
JPS4948820A (en) 1974-05-11

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
746 Register noted 'licences of right' (sect. 46/1977)
PCNP Patent ceased through non-payment of renewal fee