GB1088214A - Process for the preparation of the callicrein inactivator - Google Patents

Process for the preparation of the callicrein inactivator

Info

Publication number
GB1088214A
GB1088214A GB1105666A GB1105666A GB1088214A GB 1088214 A GB1088214 A GB 1088214A GB 1105666 A GB1105666 A GB 1105666A GB 1105666 A GB1105666 A GB 1105666A GB 1088214 A GB1088214 A GB 1088214A
Authority
GB
United Kingdom
Prior art keywords
inactivator
mgo
precipitate
acetone
callicrein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1105666A
Inventor
Gerhardt Schaefer
Klaus Piesche
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AWD Pharma GmbH and Co KG
Original Assignee
Arzneimittelwerk Dresden GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arzneimittelwerk Dresden GmbH filed Critical Arzneimittelwerk Dresden GmbH
Priority to GB1105666A priority Critical patent/GB1088214A/en
Publication of GB1088214A publication Critical patent/GB1088214A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/55—Protease inhibitors
    • A61K38/57—Protease inhibitors from animals; from humans

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Crude preparations of the callicrein inactivator from the lungs of cattle are purified by stirring an aqueous solution containing the inactivator (and having a degree of activity of 80-140 ATU/mg. of dry material) with 3-10% by weight of MgO at 10-50 DEG C. for 1-16 hours, the MgO charged with contaminating proteins and colouring matter being then separated and the solution containing the inactivator so obtained being mixed with 2-6 times its volume of a C1-C3 aliphatic alcohol or acetone to precipitate the inactivator. The inactivator solution obtained after removal of MgO may be treated with a cation exchange resin to remove any Mg ions present. The aliphatic alcohol or acetone used for precipitation may first be cooled to -5 DEG C. The precipitate finally obtained may be worked up to a dry powder by washing with ethanol or acetone and then drying. Alternatively, the precipitate may be dissolved in pyrogen-free water or in physiological NaCl solution and the solution obtained may then be sterilised by filtration.
GB1105666A 1966-03-14 1966-03-14 Process for the preparation of the callicrein inactivator Expired GB1088214A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB1105666A GB1088214A (en) 1966-03-14 1966-03-14 Process for the preparation of the callicrein inactivator

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1105666A GB1088214A (en) 1966-03-14 1966-03-14 Process for the preparation of the callicrein inactivator

Publications (1)

Publication Number Publication Date
GB1088214A true GB1088214A (en) 1967-10-25

Family

ID=9979193

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1105666A Expired GB1088214A (en) 1966-03-14 1966-03-14 Process for the preparation of the callicrein inactivator

Country Status (1)

Country Link
GB (1) GB1088214A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4190573A (en) * 1974-02-14 1980-02-26 Behringwerke Aktiengesellschaft Process for the isolation of the polyvalent proteinase inhibitor

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4190573A (en) * 1974-02-14 1980-02-26 Behringwerke Aktiengesellschaft Process for the isolation of the polyvalent proteinase inhibitor

Similar Documents

Publication Publication Date Title
Marrian The chemistry of oestrin: An improved method of preparation and the isolation of crystalline material
GB1121636A (en) Improved wound healing compositions
Schaefer Hypophysectomy and Thyroidectomy of Snakes.
Poole Spectrophotometric identification of eggshell pigments and timing of superficial pigment deposition in the Japanese quail
US2162863A (en) Stable products shortening the bleeding period
Saunders et al. Antibody synthesis initiated in vitro by paired explants of spleen and thymus
GB1088214A (en) Process for the preparation of the callicrein inactivator
GB1018528A (en) New physiologically active protein fractions from mistletoe and process for their preparation
ES358119A1 (en) Cosmetic therapeutic preparations containing animal organ extracts
GB1062434A (en) Process for isolating -antitrypsin
GB1135528A (en) Improvements in the treatment of senna pods
US2892754A (en) Process of preparing vitamin b12-intrinsic factor
DE2724918A1 (en) Antiviral and antitumour human interferon prodn. - by incubating virus-infected namalva cells on serum-free medium
GB1229570A (en)
US3308026A (en) Process for the production and recovery of the kallikrein-inacti-vator from proteinaceous hormone wastes
DE1084433B (en) Process for the production of pure solutions of the kallikrein inactivator
GB906819A (en) Parenteral solution for diagnostic test of cancer and a process of preparing same
GB532301A (en) Improvements in and relating to the preparation of sodium bicarbonate and mixtures containing sodium bicarbonate
Takeuchi Staining of sulfated mucopolysaccharides on filter paper by means of acriflavine
AT224807B (en) Method for obtaining a new anti-tumor polysaccharide
US2049134A (en) Method of making bilirubin
GB715821A (en) Improvements relating to the manufacture of sulphuric esters of dextran
DE947193C (en) Process for obtaining vitamin B.
DE950959C (en) Process for obtaining a kallikrein inactivator
GB479487A (en) Manufacture of stable therapeutic products from intestinal mucous membrane