GB0415999D0 - Proteins - Google Patents

Proteins

Info

Publication number
GB0415999D0
GB0415999D0 GBGB0415999.2A GB0415999A GB0415999D0 GB 0415999 D0 GB0415999 D0 GB 0415999D0 GB 0415999 A GB0415999 A GB 0415999A GB 0415999 D0 GB0415999 D0 GB 0415999D0
Authority
GB
United Kingdom
Prior art keywords
enzyme
variant
amino acid
optionally
acyltransferase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
GBGB0415999.2A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DuPont Nutrition Biosciences ApS
Danisco US Inc
Original Assignee
Danisco AS
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/IB2004/000575 external-priority patent/WO2004064987A2/en
Application filed by Danisco AS, Danisco US Inc filed Critical Danisco AS
Priority to US10/911,160 priority Critical patent/US20050196766A1/en
Publication of GB0415999D0 publication Critical patent/GB0415999D0/en
Priority to BRPI0417533A priority patent/BRPI0417533B1/en
Priority to NZ547085A priority patent/NZ547085A/en
Priority to JP2006546409A priority patent/JP5697294B2/en
Priority to CN200910206577A priority patent/CN101676390A/en
Priority to DK09011127.9T priority patent/DK2119771T3/en
Priority to AU2004312215A priority patent/AU2004312215B2/en
Priority to KR1020067008846A priority patent/KR101169265B1/en
Priority to PCT/IB2004/004378 priority patent/WO2005066347A1/en
Priority to EP04806537.9A priority patent/EP1704236B1/en
Priority to DK04806537.9T priority patent/DK1704236T3/en
Priority to EP09011127.9A priority patent/EP2119771B1/en
Priority to CA002550800A priority patent/CA2550800A1/en
Priority to CN201310150130.9A priority patent/CN103275944B/en
Priority to ZA2006/05158A priority patent/ZA200605158B/en
Priority to US11/852,274 priority patent/US7906307B2/en
Priority to US12/040,721 priority patent/US8030044B2/en
Priority to JP2010022832A priority patent/JP2010142243A/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Fats And Perfumes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a method of producing a variant glycolipid acyltransferase enzyme comprising: (a) selecting a parent enzyme which is a glycolipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S; (b) modifying one or more amino acids to produce a variant glycolipid acyltransferase; (c) testing the variant glycolipid acyltransferase for transferase activity, an optionally hydrolytic activity, on a galactolipid substrate, and optionally a phospholipid substrate and/or optionally a triglyceride substrate; (d) selecting a variant enzyme with an enhanced activity towards galactolipids compared with the parent enzyme; and optionally (e) preparing a quantity of the variant enzyme. The present invention further relates to variant lipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S, and wherein the variant enzyme comprises one or more amino acid modifications compared with a parent sequence at any one or more of the amino acid residues defined in set 2, set 4, set 6 or set 7.
GBGB0415999.2A 2003-12-24 2004-07-16 Proteins Ceased GB0415999D0 (en)

Priority Applications (18)

Application Number Priority Date Filing Date Title
US10/911,160 US20050196766A1 (en) 2003-12-24 2004-08-02 Proteins
CA002550800A CA2550800A1 (en) 2003-12-24 2004-12-23 Methods to produce variant glycolipid acyltransferases and variant glycolipid acyltransferases with enhanced activity towards glycolipids.
CN201310150130.9A CN103275944B (en) 2003-12-24 2004-12-23 Protein
KR1020067008846A KR101169265B1 (en) 2003-12-24 2004-12-23 Proteins
DK04806537.9T DK1704236T3 (en) 2003-12-24 2004-12-23 PROTEINS
JP2006546409A JP5697294B2 (en) 2003-12-24 2004-12-23 Glycolipid acyltransferase mutant and method for producing the same
CN200910206577A CN101676390A (en) 2003-12-24 2004-12-23 Variant glycolipid acyltransferase enzyme and use thereof
DK09011127.9T DK2119771T3 (en) 2003-12-24 2004-12-23 PROTEINS
AU2004312215A AU2004312215B2 (en) 2003-12-24 2004-12-23 Proteins
BRPI0417533A BRPI0417533B1 (en) 2003-12-24 2004-12-23 variant enzyme glycolipid acyltransferase, use and method for its production, method for preparing a food product and flour baked product, enzymatic refinement process
PCT/IB2004/004378 WO2005066347A1 (en) 2003-12-24 2004-12-23 Proteins
EP04806537.9A EP1704236B1 (en) 2003-12-24 2004-12-23 Proteins
NZ547085A NZ547085A (en) 2003-12-24 2004-12-23 Proteins
EP09011127.9A EP2119771B1 (en) 2003-12-24 2004-12-23 Proteins
ZA2006/05158A ZA200605158B (en) 2003-12-24 2006-06-22 Proteins
US11/852,274 US7906307B2 (en) 2003-12-24 2007-09-07 Variant lipid acyltransferases and methods of making
US12/040,721 US8030044B2 (en) 2003-12-24 2008-02-29 Lipid acyltransferases
JP2010022832A JP2010142243A (en) 2003-12-24 2010-02-04 Lipid acyl group transferase variant improved in phospholipid transferase activity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0330016.7A GB0330016D0 (en) 2003-12-24 2003-12-24 Method
PCT/IB2004/000575 WO2004064987A2 (en) 2003-01-17 2004-01-15 Method

Publications (1)

Publication Number Publication Date
GB0415999D0 true GB0415999D0 (en) 2004-08-18

Family

ID=30776521

Family Applications (3)

Application Number Title Priority Date Filing Date
GBGB0330016.7A Ceased GB0330016D0 (en) 2003-01-14 2003-12-24 Method
GBGB0415999.2A Ceased GB0415999D0 (en) 2003-12-24 2004-07-16 Proteins
GBGB0416023.0A Ceased GB0416023D0 (en) 2003-12-24 2004-07-16 Method

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GBGB0330016.7A Ceased GB0330016D0 (en) 2003-01-14 2003-12-24 Method

Family Applications After (1)

Application Number Title Priority Date Filing Date
GBGB0416023.0A Ceased GB0416023D0 (en) 2003-12-24 2004-07-16 Method

Country Status (10)

Country Link
JP (1) JP2010142243A (en)
CN (2) CN101676390A (en)
AT (1) ATE462011T1 (en)
DE (1) DE602004026229D1 (en)
DK (1) DK1704236T3 (en)
GB (3) GB0330016D0 (en)
HK (1) HK1106953A1 (en)
NZ (1) NZ547085A (en)
RU (2) RU2377300C2 (en)
ZA (1) ZA200605158B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114958800B (en) * 2022-06-24 2023-08-25 北京脉道生物药品制造有限公司 Taq DNA polymerase mutant capable of tolerating blood or blood product inhibition and application thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69825263T3 (en) * 1997-04-09 2009-03-12 Danisco A/S LIPASE AND USE THEREOF FOR IMPROVING PASTE AND BAKERY PRODUCTS
ATE231186T1 (en) * 1998-07-21 2003-02-15 Danisco GROCERIES
DE50115482D1 (en) * 2001-07-11 2010-06-24 Cognis Ip Man Gmbh Lipase / acyltransferase from Candida parapsilosis
EP1660648B1 (en) * 2003-08-11 2013-10-09 Codexis, Inc. Improved glucose dehydrogenase polypeptides and related polynucleotides

Also Published As

Publication number Publication date
ATE462011T1 (en) 2010-04-15
NZ547085A (en) 2009-11-27
HK1106953A1 (en) 2008-03-20
GB0330016D0 (en) 2004-01-28
RU2006126715A (en) 2008-01-27
ZA200605158B (en) 2008-04-30
DE602004026229D1 (en) 2010-05-06
CN103275944B (en) 2016-12-07
DK1704236T3 (en) 2018-01-08
RU2377300C2 (en) 2009-12-27
RU2377307C2 (en) 2009-12-27
GB0416023D0 (en) 2004-08-18
CN103275944A (en) 2013-09-04
JP2010142243A (en) 2010-07-01
CN101676390A (en) 2010-03-24
RU2006126654A (en) 2008-01-27

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Legal Events

Date Code Title Description
AT Applications terminated before publication under section 16(1)