GB0416023D0 - Method - Google Patents
MethodInfo
- Publication number
- GB0416023D0 GB0416023D0 GBGB0416023.0A GB0416023A GB0416023D0 GB 0416023 D0 GB0416023 D0 GB 0416023D0 GB 0416023 A GB0416023 A GB 0416023A GB 0416023 D0 GB0416023 D0 GB 0416023D0
- Authority
- GB
- United Kingdom
- Prior art keywords
- enzyme
- variant
- amino acid
- optionally
- acyltransferase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Fats And Perfumes (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
Abstract
The present invention relates to a method of producing a variant glycolipid acyltransferase enzyme comprising: (a) selecting a parent enzyme which is a glycolipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S; (b) modifying one or more amino acids to produce a variant glycolipid acyltransferase; (c) testing the variant glycolipid acyltransferase for transferase activity, an optionally hydrolytic activity, on a galactolipid substrate, and optionally a phospholipid substrate and/or optionally a triglyceride substrate; (d) selecting a variant enzyme with an enhanced activity towards galactolipids compared with the parent enzyme; and optionally (e) preparing a quantity of the variant enzyme. The present invention further relates to variant lipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S, and wherein the variant enzyme comprises one or more amino acid modifications compared with a parent sequence at any one or more of the amino acid residues defined in set 2, set 4, set 6 or set 7.
Priority Applications (20)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/898,775 US7718408B2 (en) | 2003-12-24 | 2004-07-26 | Method |
NZ547082A NZ547082A (en) | 2003-12-24 | 2004-12-23 | Enzymatic treatment of oils |
AT04806534T ATE376593T1 (en) | 2003-12-24 | 2004-12-23 | ENZYMATIC TREATMENT OF OILS |
EP07017066A EP1862554B1 (en) | 2003-12-24 | 2004-12-23 | Enzymatic removal of diglycerides from edible oils |
CA2550789A CA2550789C (en) | 2003-12-24 | 2004-12-23 | Enzymatic treatment of oils |
DE602004026229T DE602004026229D1 (en) | 2003-12-24 | 2004-12-23 | ENZYMATIC REMOVAL OF DIGLYCERIDES FROM EDIBLE OILS |
BRPI0418107-7A BRPI0418107A (en) | 2003-12-24 | 2004-12-23 | enzymatic treatment of oils |
AT07017066T ATE462011T1 (en) | 2003-12-24 | 2004-12-23 | ENZYMATIC REMOVAL OF DIGLYCERIDES FROM COOKING OILS |
PCT/IB2004/004374 WO2005066351A2 (en) | 2003-12-24 | 2004-12-23 | Enzymatic treatment of oils |
EP04806534A EP1704240B1 (en) | 2003-12-24 | 2004-12-23 | Enzymatic treatment of oils |
AU2004312213A AU2004312213B2 (en) | 2003-12-24 | 2004-12-23 | Enzymatic Treatment of Oils |
CNA2004800386995A CN1898386A (en) | 2003-12-24 | 2004-12-23 | Proteins |
JP2006546408A JP4949042B2 (en) | 2003-12-24 | 2004-12-23 | Enzymatic treatment of cooking oil |
DK04806534T DK1704240T3 (en) | 2003-12-24 | 2004-12-23 | Enzymatic treatment of oils |
ES04806534T ES2294575T3 (en) | 2003-12-24 | 2004-12-23 | ENZYMATIC TREATMENT OF OILS. |
CNA2004800389334A CN1898391A (en) | 2003-12-24 | 2004-12-23 | Enzymatic treatment of oils |
DE602004009713T DE602004009713T2 (en) | 2003-12-24 | 2004-12-23 | ENZYMATIC TREATMENT OF OILS |
HK06113532A HK1091868A1 (en) | 2003-12-24 | 2006-12-08 | Enzymatic treatment of oils |
HK08100607.7A HK1106953A1 (en) | 2003-12-24 | 2008-01-17 | Enzymatic removal of diglycerides from edible oils |
US12/492,042 US8440435B2 (en) | 2003-12-24 | 2009-06-25 | Method for reducing 1,2-diglyceride content of an edible oil |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0330016.7A GB0330016D0 (en) | 2003-12-24 | 2003-12-24 | Method |
PCT/IB2004/000655 WO2004064537A2 (en) | 2003-01-17 | 2004-01-15 | Method for the in situ production of an emulsifier in foodstuff |
Publications (1)
Publication Number | Publication Date |
---|---|
GB0416023D0 true GB0416023D0 (en) | 2004-08-18 |
Family
ID=30776521
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GBGB0330016.7A Ceased GB0330016D0 (en) | 2003-01-14 | 2003-12-24 | Method |
GBGB0415999.2A Ceased GB0415999D0 (en) | 2003-12-24 | 2004-07-16 | Proteins |
GBGB0416023.0A Ceased GB0416023D0 (en) | 2003-12-24 | 2004-07-16 | Method |
Family Applications Before (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GBGB0330016.7A Ceased GB0330016D0 (en) | 2003-01-14 | 2003-12-24 | Method |
GBGB0415999.2A Ceased GB0415999D0 (en) | 2003-12-24 | 2004-07-16 | Proteins |
Country Status (10)
Country | Link |
---|---|
JP (1) | JP2010142243A (en) |
CN (2) | CN103275944B (en) |
AT (1) | ATE462011T1 (en) |
DE (1) | DE602004026229D1 (en) |
DK (1) | DK1704236T3 (en) |
GB (3) | GB0330016D0 (en) |
HK (1) | HK1106953A1 (en) |
NZ (1) | NZ547085A (en) |
RU (2) | RU2377300C2 (en) |
ZA (1) | ZA200605158B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114958800B (en) * | 2022-06-24 | 2023-08-25 | 北京脉道生物药品制造有限公司 | Taq DNA polymerase mutant capable of tolerating blood or blood product inhibition and application thereof |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69835112T2 (en) * | 1997-04-09 | 2007-01-04 | Danisco A/S | Use of lipase to improve doughs and baked goods |
WO2000005396A1 (en) * | 1998-07-21 | 2000-02-03 | Danisco A/S | Foodstuff |
DE50115482D1 (en) * | 2001-07-11 | 2010-06-24 | Cognis Ip Man Gmbh | Lipase / acyltransferase from Candida parapsilosis |
JP2007502114A (en) * | 2003-08-11 | 2007-02-08 | コデクシス, インコーポレイテッド | Improved glucose dehydrogenase polypeptides and related polynucleotides |
-
2003
- 2003-12-24 GB GBGB0330016.7A patent/GB0330016D0/en not_active Ceased
-
2004
- 2004-07-16 GB GBGB0415999.2A patent/GB0415999D0/en not_active Ceased
- 2004-07-16 GB GBGB0416023.0A patent/GB0416023D0/en not_active Ceased
- 2004-12-23 CN CN201310150130.9A patent/CN103275944B/en not_active Expired - Fee Related
- 2004-12-23 NZ NZ547085A patent/NZ547085A/en not_active IP Right Cessation
- 2004-12-23 DE DE602004026229T patent/DE602004026229D1/en active Active
- 2004-12-23 DK DK04806537.9T patent/DK1704236T3/en active
- 2004-12-23 CN CN200910206577A patent/CN101676390A/en active Pending
- 2004-12-23 AT AT07017066T patent/ATE462011T1/en not_active IP Right Cessation
- 2004-12-23 RU RU2006126715/13A patent/RU2377300C2/en not_active IP Right Cessation
- 2004-12-23 RU RU2006126654/13A patent/RU2377307C2/en not_active IP Right Cessation
-
2006
- 2006-06-22 ZA ZA2006/05158A patent/ZA200605158B/en unknown
-
2008
- 2008-01-17 HK HK08100607.7A patent/HK1106953A1/en not_active IP Right Cessation
-
2010
- 2010-02-04 JP JP2010022832A patent/JP2010142243A/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CN103275944B (en) | 2016-12-07 |
RU2006126715A (en) | 2008-01-27 |
CN101676390A (en) | 2010-03-24 |
RU2377300C2 (en) | 2009-12-27 |
CN103275944A (en) | 2013-09-04 |
ATE462011T1 (en) | 2010-04-15 |
RU2377307C2 (en) | 2009-12-27 |
JP2010142243A (en) | 2010-07-01 |
RU2006126654A (en) | 2008-01-27 |
DE602004026229D1 (en) | 2010-05-06 |
GB0330016D0 (en) | 2004-01-28 |
HK1106953A1 (en) | 2008-03-20 |
NZ547085A (en) | 2009-11-27 |
GB0415999D0 (en) | 2004-08-18 |
DK1704236T3 (en) | 2018-01-08 |
ZA200605158B (en) | 2008-04-30 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
AT | Applications terminated before publication under section 16(1) |