FR3061208B1 - Adjuvants pour milieux de culture cellulaire, notamment pour fecondation in vitro, ou pour la culture de follicules, cellules germinales males ou embryons - Google Patents

Adjuvants pour milieux de culture cellulaire, notamment pour fecondation in vitro, ou pour la culture de follicules, cellules germinales males ou embryons Download PDF

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Publication number
FR3061208B1
FR3061208B1 FR1663354A FR1663354A FR3061208B1 FR 3061208 B1 FR3061208 B1 FR 3061208B1 FR 1663354 A FR1663354 A FR 1663354A FR 1663354 A FR1663354 A FR 1663354A FR 3061208 B1 FR3061208 B1 FR 3061208B1
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France
Prior art keywords
follicles
embryos
adjuvants
culture media
germ cells
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FR1663354A
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English (en)
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FR3061208A1 (fr
Inventor
Patrick Choay
Mohamed Bidri
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Patrick Choay Sas
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Patrick Choay Sas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Patrick Choay Sas filed Critical Patrick Choay Sas
Priority to FR1663354A priority Critical patent/FR3061208B1/fr
Priority to PCT/FR2017/053816 priority patent/WO2018115788A1/fr
Publication of FR3061208A1 publication Critical patent/FR3061208A1/fr
Application granted granted Critical
Publication of FR3061208B1 publication Critical patent/FR3061208B1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0604—Whole embryos; Culture medium therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C12N2500/33—Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/35—Polyols, e.g. glycerin, inositol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Reproductive Health (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention a pour objet des compositions pour la culture in vitro de cellules, caractérisées en ce qu'elles comprennent au moins un facteur de croissance en association avec au moins un composé choisi parmi le groupe constitué par un sel de zinc, l'inositol sous l'une quelconque de ses formes stéréoisomériques et la carnitine ou un dérivé de la carnitine. L'invention a également pour objet des procédés de fécondation in vitro et des procédés de maturation de follicules, de cellules germinales mâles, ou d'embryons utilisant de tels adjuvants de milieux de culture, ainsi que des kits pour la mise en œuvre de ces procédés et comprenant un lyophilisat susmentionné et une solution aqueuse, contenant les autres constituants desdits milieux de culture.
FR1663354A 2016-12-23 2016-12-23 Adjuvants pour milieux de culture cellulaire, notamment pour fecondation in vitro, ou pour la culture de follicules, cellules germinales males ou embryons Active FR3061208B1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FR1663354A FR3061208B1 (fr) 2016-12-23 2016-12-23 Adjuvants pour milieux de culture cellulaire, notamment pour fecondation in vitro, ou pour la culture de follicules, cellules germinales males ou embryons
PCT/FR2017/053816 WO2018115788A1 (fr) 2016-12-23 2017-12-22 Adjuvants pour milieux de culture cellulaire, notamment pour fecondation in vitro, ou pour la culture de follicules, cellules germinales males ou embryons

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1663354A FR3061208B1 (fr) 2016-12-23 2016-12-23 Adjuvants pour milieux de culture cellulaire, notamment pour fecondation in vitro, ou pour la culture de follicules, cellules germinales males ou embryons
FR1663354 2016-12-23

Publications (2)

Publication Number Publication Date
FR3061208A1 FR3061208A1 (fr) 2018-06-29
FR3061208B1 true FR3061208B1 (fr) 2024-04-05

Family

ID=58737662

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1663354A Active FR3061208B1 (fr) 2016-12-23 2016-12-23 Adjuvants pour milieux de culture cellulaire, notamment pour fecondation in vitro, ou pour la culture de follicules, cellules germinales males ou embryons

Country Status (2)

Country Link
FR (1) FR3061208B1 (fr)
WO (1) WO2018115788A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2019278918A1 (en) * 2018-05-29 2020-12-24 NuLife B.V. Method for enhancing embryo implantation
CN111280163B (zh) * 2020-02-12 2021-11-09 天津博裕力牧科技有限公司 牛活体采卵用的抽卵液及其制备方法和用途

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003517276A (ja) * 1998-11-30 2003-05-27 アイ・ブイ・エフ・サイエンシイズ・コロラド・インコーポレーテツド 体外受精のための系及び連続的培養液
FR2812004B1 (fr) * 2000-07-24 2002-12-27 Ccd Lab Milieux de culture pour fecondation in vitro, ou pour la culture de follicules, cellules germinales males ou embryons
JP2005518823A (ja) * 2002-03-08 2005-06-30 マクギル・ユニバーシテイ 未成熟ヒト卵母細胞の体外成熟

Also Published As

Publication number Publication date
FR3061208A1 (fr) 2018-06-29
WO2018115788A1 (fr) 2018-06-28

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