FR2885296A1 - Oral or topical composition having antioxidant, anti oxygenated free radicals and anti rheumatic properties, useful in day or night formulations to fight against wrinkles and ridules, comprises oils and extracts of Astrocaryum vulgare fruit - Google Patents

Abstract

Oral or topical composition having antioxidant, anti oxygenated free radicals and anti rheumatic properties, comprising oils and extracts of Astrocaryum vulgare fruit (I), is new. ACTIVITY : Dermatological; Antirheumatic. No biological data given. MECHANISM OF ACTION : Antioxidant.

Description

ANTIOXIDANT PROPERTIES, ANTIRADICAL AND ANTI-RAESTURANTS RHEUMATISMALES

  OILS AND THE ASTROCARYUM VULGARE SWEAT.

  The present invention relates to the antioxidant, oxygenated free antiradical and rheumatic stiffness properties of Astrocaryum vulgare pulp and almond oils and the antioxidant properties of Astrocaryum vulgare almond cake.

  The applications of these properties concern the field of animal and human health, the pharmaceutical, nutraceutical, cosmetic and dermocosmetic fields, in oral or topical form.

  Oxidative stress is defined as the result of an imbalance in the body between the balance of prooxidants and antioxidant defense systems, resulting in the appearance of often irreversible damage to the cell. The antioxidant potential of each individual is variable according to his lifestyle, his state of stress, his diet, his environment. (Betteridge DJ: What is oxidative stress? Metabolism, 2000 Feb; 49 {2 Suppl 1): 3-8.) The reactive oxygen species are constantly produced by cellular metabolism at the level of the mitochondria.

  (Fariss MW, CB Chan, Patel M, Van Houten B, Orrenius S. Role of mitochondria in toxic oxidative stress.Mol Interv 2005 Apr; 5 (2): 94-111.) Sometimes some of this oxygen escapes complete transformation and gives oxygenated free species, toxic for cellular integrity. These reactive oxygen species, which include free radicals, for example the superoxide anion, are produced in a chain, are no longer removed by the biochemical and enzymatic defense systems, and will interact with biological substrates that may involve the development of various pathologies. (Harper ME, Bevilacqua L, Hagopian K, Weindruch R, Ramsey JJ.Ageing, oxidative stress, and mitochondrial uncoupling.Acta Physiol Scand 2004 Dec; 182 (4): 321-31.) Oxygen free radicals can cause damage at several levels of the body: - In lipid compounds, the membrane bilayer then loses its capacity to transport oxygen, nutrients, water to the cell; - Protein compounds: loss of cell regulation, growth and tissue regulation functions by attacking nucleic acids; -In cellular compounds, chromosome and nucleic acid damage can lead to uncontrolled cellular growth and apoptosis phenomena; - Lysosomes, vesicles contain degenerative enzymes that after breaking the membrane spread in the cell and destroy it, causing a chain reaction that weakens the immune system; -In the pathophysiology of aging, cataracts, arteriosclerosis, osteoporosis, premature aging, cardiovascular diseases, diabetes, psoriasis, skin disorders, chronic inflammatory diseases, Alzheimer's, tumors ..

  These oxygenated free species are also generated by the harmful effects of the environment (UV, pollution, ionizing radiation, tobacco, alcohol, drugs).

  To protect themselves, the body uses its own defense systems. When defense systems are overwhelmed by hyperproduction of free radicals, the body must be provided with antioxidant substances. These molecules of enzymatic nature, or not, are not synthesized by the body, and they must be drawn into the diet.

  (Ferrari CK, Torres EA.) Biochemical pharmacology of functional foods and prevention of chronic diseases of aging.

  Biomed Pharmacother. 2003 Jul-Aug; 57 (5-6): 251-60.) Different compounds are active on the oxidation phenomena: Vitamins (A, C, E), their precursors and coenzymes, flavonoids, minerals and trace elements (Copper, Zinc, Selenium), lycopene, limonem, glutathione, ferritin, ceruloplasmin, lipoic acid These antioxidants can act by different actions: protection, capture, defense. (Lingyun Wu, Hossein Noyan Ashraf, Marina Facci, Rui Wang, Phyllis G. Paterson, Alison Ferrie, and Bernhard HJ Juurlink Dietary Approach to Adverse Oxidative Stress, Hypertension, and Inflammation in the Cardiovascular System, PNAS 1 May 4, 2004 1 vol.101 no.18 (7094-7099) (Alan C. Logan, ND, Cathy Wong, ND (Cand.) Chronic Fatigue Syndrome: Oxidative Stress and Dietary Changes, Altern Med Rev, 2001; 6 (5): 450-459) The action of oxidative stress also applies to damage to the skin and to early skin aging problems that lead to the appearance of wrinkles.The current formulations applied to anti-aging and skin care products build on recent findings regarding the metabolic alteration of the balance between collagen synthesis and destruction (Reuther T, Kerscher M Active Ingredients In Dermatocosmetics Hautarzt 2004 Jul; 55 (7): 630-6.) Skin cells renew themselves more slowly with age and syn Collagen thesis is slowing down, while degradation enzymes are working at the same pace. The consequences are a very fine skin, hard and distended, antioxidants then play the role of anti-aging assets because by allowing the fight against free radicals, they improve the relief of the skin and repair the damage caused by pollution. These measures must always be accompanied by perfect hygiene of the skin.

  The recommended solution to prevent oxidative stress and the production of free radicals is the complementation of antioxidants.

  All antioxidants do not play the same role in our body and do not act in the same way. One should not take only an estimated active amount of a single antioxidant, as other deficiencies may exist and there would be no positive effect on health.

  It should be known that the body generates the production of 10 free radicals and is also sensitive to external aggressions, especially since we consume more oxygen.

  The term rheumatism includes all pathologies that affect the musculoskeletal system, both in the joints and bones, but also in the associated structures: muscles, tendons and ligaments. These pathologies cause pain, functional disorders including stiffness and decreased mobility associated. Several types of rheumatism can be distinguished: inflammatory rheumatism, type osteoarthritis, degenerative rheumatism of wear, osteoarthritis type, and soft rheumatism. Only inflammatory type rheumatism can be influenced by diet. The substances that have an effect on inflammation are antioxidants (vitamins E, C, beta carotene, minerals, coenzymes ...). These antioxidants capture and neutralize free radicals, neutralizing inflammatory attacks and thus reducing side effects like the gene in mobility and skeletal and muscular stiffness. Antioxidants also reduce glycosylation phenomena that create cross-links of protein tissues within tendons and ligaments, hence rheumatism and arthritis with aging.

  The Astrocaryum vulgare C. Martius belongs to the family of Palmae or Arecaceae, its vernacular names are Awara in Creole, and Tucumâ in Portuguese. Its presence is very common in the coastal plain of Guyana and in the Amazonian grove. It grows in groves, on the edge of savannas on sandy soil. It takes 5 years for soil preparation, planting and the first fruit harvest. (Govaerts, R. (1995) World Checklist of Seed Plants, 1 (1, 2): 1-483, 1529. MIM, Deurne.

  Govaerts, R. & Dransfield, J. (2003). World Checklist of Palms. The cultivation of this palm in French Guiana takes place in the Iracoubo plantations, under the label Bio ECOCERT.

  Knowing that this species is subject to many variations in the composition of assets, depending on the geographical location, it is useful to use only fruits of this palm, stable in their growth medium. ((Lima et al., 1986), David Arkcoll, New Crops from Brazil, 1990, pp. 367-371. In: J. Janick and JE Simon (eds.), Advances in new crops, Timber Press, Portland, OR. ) The characteristics of Astrocaryum vulgare are the presence of a solitary stipe of 10 to 15 (less than 25) m high and 20 to 30 cm in diameter, 20 cm long internodes, armed unequal spines, black from 10 to 25 cm long From the point of view economic aspect it is a palm tree with great potential. We are mainly interested in its fruits. The production of oil is used for the self-consumption of the population. Astrocaryum vulgare is classified as a food palm (Notice Cerfa N 50861 * 02L, Dossier alimentaire) the part used for the use in pharmaceutical, nutraceutical and cosmetic formulations, is the fruit of the Astrocaryum vulgare. The fruit is an ovoid to subglobose drupe, 3.5 to 5 cm long and 2.5 to 4 cm in diameter, short-acuminate, orange-yellow in maturity or orange; glabrous having a flat cup about 2 cm in diameter, with a mesocarp more or less thick (3 to 8 mm), fleshy, surrounding an ovoid nucleus, very hard narrowing towards the base 3 mm thick, l almond is white. (Texts and plates of Mr. Gazel, CIRAD, Jean-Jacques de Granville).

  The fruits are collected directly on the tree, sterilized in saturated steam at 4 bar for 2H30mn in order to stop the activity of lipases.

  The components of the pulp are then extracted either in industrial mode by kneading in order to obtain the maximum amount of juice available on the fibers, then steam rinsing and static settling; either in analytical mode by hydro-maceration, by organic extraction: alcoholic fraction, glyceric fraction, unsaponifiable fraction. The solvents employed are ethanol, hexane, cyclohexane and ethyl ethanoate. The components of the almond are extracted after crushing the hard core, using the same techniques as for the pulp, the almond cake is then recovered and kept in a cold room. The soap is made by saponification of pulp and almond oil from El is guineensis. The water used for saponification is passed through an activated carbon filter, filter made from Astrocaryum vulgare shells. Once the saponification is carried out, it is set to be dried permanently in the open air. Once the soap has lost its water, without sequestrant, it is remelt by adding the sludge of the oils of Astrocaryum vulgare, and it is put back to dry naturally without hardener, without preservative, without antioxidant.

  From the point of view of the physicochemical and extraction properties used at the industrial level, a very good quality of the pulp oils and to the point the methods characterize almonds.

  -The pulp oil is bright red, very pleasant smell, liquid at 25 C. Iodine 80.1 +/- 1.73 (gI2 / 100g) indicates the predominant presence of unsaturated compounds, the value of the peroxide value: 1, 95 +/- 0.03 (meq / 02 / Kg) demonstrates the oxidation stability of the double bonds in the method of preparation, acid number: 2.24 + / -0.02 (mgKOH / g), refractive index: 1.4507 +/- 0.0005, melting point: 28 +/- 1 (C). The protein content is 6.4% +/- 0.2, the fiber content is 7.05% +/- 0.2, the fat content is 60.04% +/- 1.86 , the carbohydrate content is 6.465% +/- 1.87 - The almond oil is creamy white, pleasant odor, solid at 25 C. Iodine 13.2 +/- 1.43 (gI2 / 100g) indicates the majority presence of saturated compounds, the value of the peroxide value: 2.05 +/- 0.02 (meq / 02 / Kg), acid number: 2.85 +/- 0.03 (mgKOH / g), refractive index: 1.4510 +/- 0.0009, melting point: 28 +/- 1 (C). The protein content is 8.7% +/- 0.2, the fiber content is 10.08% +/- 0, 2, the lipid content is 39.02% +/- 1.74 . The carbohydrate content is 4.973% +/- 2, 15.

  The fatty acids used in the composition of Astrocaryum vulgare pulp and almond oils were determined with a Focus 12550060 Thermo Finigan gas chromatograph equipped with a Valcobond VB-225 column (50% cyanopro pylphenyl). methyl polysiloxane: 30m x 0.25mm x 0.25 Saturated fatty acid content: C12: 0 (Pulp: 0.1%, almond: 43.5%), C14: 0 (Pulp: 0.2%, almond: 28.6%), C16: 0 (Pulp: 25, 3%, almond: 7.5%), C18: 0 (Pulp: 1.6%, almond: 3.0%), C20 : 0 (Pulp: 0.15%, almond: 0.1%) Unsaturated fatty acid content: C16: 1 (Pulp: 0.4%, almond: 13.6%), C18: 1 (Pulp : 67.1%, almond: 3.3%), C18: 2 (Pulp: 3.1%, almond: 0.15%), C18: 3 (Pulp: 2.5%, almond: 0, 15%).

  Unsaturated fatty acids have a role as manufacturers of membrane phospholipids, a role of cellular mediators and an energetic role. They allow the skin to maintain resistance and elasticity and thus prevent skin aging. Pulp oils contain about 70% oleic acid, and linoleic and linolenic acids. These essential fatty acids help to nourish and moisturize the skin, so to fight against skin dryness that promotes the appearance of wrinkles.

  Extraction of the unsaponifiable fraction after complete saponification of the oil according to the AFNOR NFT60205 standard, preparative TLC isolation and analysis by WATERS ALLIANCE HPLC, separation module 2690, diode array detector 996, MILLENNIUM software, Column C18 Supelcosil LC-18 120 Å, 5 cm cm 4,6 4.6 mm, Pre column Supelcosil LC-18 2 cm x 4.0 mm 25 .: very little cholesterol: traces, (3-sitosterol: 196-260 ppm, campesterol: 60-80 ppm, stigmasterol: 46-62 ppm, fucosterol: 196-260 ppm Analysis of tocopherols: 41-45 mg / Kg on total oil (pulp + almond), tocotrienols: 55-59 mg / Kg almond oil, Carotenoids: 28% pulp oil, 13% almond oil, Cycloarthenol: 61.4% and Lanosta-8.24-diene beta-ol + 24methylene cycloarthanol: 38.6%.

  These compounds have curative actions in dermocosmetics and are directly related to sun protection and the maintenance of tissue integrity.

  The amino acids constituting the almond meal of Astrocaryum vulgare were analyzed by thin-layer chromatography of cellulose on an aluminum support, the solvent used is butanol, acetic acid, water (70, 18, 12, v% ) developer: ninhydrin 1% in acetone. Glutamic acid is predominant (30%), followed by arginine (15%), aspartic acid (10%), leucine and glycine (5%), serine, phenylalanine, alanine, serine, proline (4%). The other amino acids are in smaller quantities. This balance makes it possible to use almond cake in human, animal and cosmetic health.

  Analysis of the bactericidal effect of certain isolated fractions of Astrocaryum vulgare: the study was carried out on four fractions by microbiological analysis: Unsaponifiable fraction of almond oil, Hydro distillate of essential oil of almond, Essential oil almond, aqueous extract of almond powder decoction. The extracts were spotted on disks to control the pharmacopoeial claim for furunculosis: ICH standard test on Staphylococcus aureus strains (2), Staphylococcus epidermis, Streptococcus A, Streptococcus B, Pseudomonas aeruginosa. Activity revealed: Aqueous extract of decoction of almond powder on Staphylococcus aureus, Staphylococcus epidermis, Streptococcus B. (Concentration of the extract: 66g / L, Concentration of the deposit: 33ng) The extract of almond powder incorporated into the Guyana soaps (the little Moucaya, horsehair glove from the Amazon, Mamaribo), combines the guarantees of antimicrobial hygiene with the antioxidant properties of oils.

  Oxidative stress analysis, in vitro by Eurochrom HPLC software on whole oil fractions: The oxidative stress test gave values of 187 000 nmol.l-1 of coenzyme Q9 and 62 000 nmol.L-1 for coenzyme 410 for the reduced forms, very low values for the oxidized forms, ie 20.47 nmol. L-1 for coenzyme Q9 and 2.2 nmol. L-1 for coenzyme Q10. Tocopherols (predominant form: atocopherol 95%) gave values of 109 pmoles. L 1 for the reduced form and 4.5 pmoles.L-1 for the oxidized form. The reduced R-carotene form gave 32 mg. L-1.

  Coenzymes Q9 and Q10 are energy generators and antioxidants. Q9 and Q10 coenzymes are immunostimulants, they promote circulation, have anti-aging effects, and bring benefits to the cardiovascular system. Coenzyme Q9 is provided by plants.

  They are both associated with the properties of tocopherols and their rates decrease with age, thus decreasing the vitality of cells and consequently anabolism. They are essential to promote an active synthesis of collagen and elastin. Vitamin A The anti-elastase test was carried out on pulp oil, almond oil and almond meal of Astrocaryum vulgare by the ELISA technique, in an elastase buffer (Tris HCl, elastase, N-succinyl ala-ala -alpha-nitroanilide), the inhibitor used being PMSF in isopropanol. The studies were carried out at 405 nm, the incubation time retained was 10 minutes, the assay was conducted over 15 minutes with measurements every 3 minutes, the analysis was performed relative to the positive buffers and buffers extracts. The pulp oils were extracted with hexane, preferred ratio 1: 2, the almond oils with hexane and acetonitrile, preferred ratio 1: 0.5: 0.5, the almond powder was extracted by 95% ethanol (lg / 10ml). The percentage inhibition was 100% for the pulp oil, 73% for the almond oil, 94% for the almond powder extract.

  Examples of uses of Astrocaryum vulgare oils and powders: 1. Use of an oral anti-wrinkle formulation: the protocol consists in taking soft gel capsules, morning and evening. Gelatin capsules (animal, vegetable origin) will comply with the legislation in force, containing no specified risk material, they may be colored (example yellow iron oxide or titanium, indigotine). The dosage will be 1 to 2 day capsules and 1 to 2 night capsules daily for 8 weeks minimum, the capacity of the capsules is 1 ml. The presentation would be in boxes containing 60 or 120 day / night capsules.

  = Daytime intake consists of a capsule of Astrocaryum vulgare pulp oil. The pulp oil has a percentage of 100% inhibition on the elastase activity.

  Pulp oil contains a very high dose of vitamin A. This vitamin is mainly used for the formation and maintenance of the skin and mucous membranes. It acts as 5-Carotene as an antioxidant active in apoptosis phenomena. The in vitro test of oxidative stress shows that the amount of 13-Carotene contributed corresponds to ER at 5333 ER / 100g, ie 2.6 times more than the dose provided by the carrot. Interest: restore collagen synthesis. Q coenzymes are substances whose role is to accelerate chemical reactions, provide energy to cells and maintain the integrity of the immune system. Their chemical structures, similar to vitamin E, give them the ability to be powerful antioxidants. They are able to inhibit the lipid peroxidation of mitochondrial membranes, a peroxidation that attacks cell membranes and severely limits energy-generating potential. Vitamin E will play a role in all diseases where oxidation occurs. Unsaturated fatty acids have a role of constructing membrane phospholipids, a role of cellular mediators and an energetic role. They allow the skin to maintain resistance and elasticity and thus prevent skin aging. The presence of a large amount of 13-sitosterol (196-260 ppm), stimulates the immune process, limit the proliferation of inflammatory processes and carcinogens, and associate its action with antioxidants in the drift pathologies of aging . It intervenes at the level of the anti-wrinkle treatment as associated in the maintenance of the dermal cellular integrity. Pulp oils contain about 70% oleic acid, and linoleic and linolenic acids. These essential fatty acids help to nourish and moisturize the skin, so to fight against skin dryness that promotes the appearance of wrinkles.

  Nighttime intake consists of an almond oil capsule of Astrocaryum vulgare. Almond oil has a 73% inhibition percentage on elastase activity.

  Almond oil brings tocopherols and tocotrienols. Vitamin E is the sum of 8 different forms, all essential to the body. It is a powerful antioxidant that fights against the spread of chain reactions initiated by RLO. In particular, it acts on the integrity and stability of cell membranes and lipoproteins. Tocotrienols are distinguished by rapid penetration into the skin and a fight against damage caused by the environment (photo aging). The in vitro oxidative stress test gives a tocopherol + tocotrienol value of 109 pmol / L, ie a dose of 16.8 mg, corresponding to a value close to the RDA.

  The nighttime dose may consist of a capsule, a hard or soft capsule, or a tablet of almond powder. The active ingredient may according to the dosage form envisaged be mixed with a vector (wheat starch, lactose, sucrose, sorbitol, talc, magnesium stearate, stearic acid, calcium diphosphate, gums, agaragar powder). This protein powder has an anti-elastase activity of 94%. This deoiled powder has the advantage of being devoid of fatty acids, of saturated origin, with respect to the oil, to confer a protein intake, in essential amino acids, which play a role in the pathology of aging of the integuments , mucous membranes and skin.

  2. Use of a food form of Astrocaryum vulgare oil. Astrocaryum vulgare pulp oil may be blended in percentages ranging from 5% to 95% to El is guineensis oil, for dietary purposes, as table oil or as a base for making biscuits and margarines. The combination of these two oils provides cellular regenerative properties, thanks to their unsaponifiable content (> 1%) and their composition in antioxidant molecules, and oleic acid.

  3. Topical use of astrocaryum vulgare oil to combat environmental damage and age-related damage to the skin of the face, neck and hands. Topical preparations may be in the form of creams, gels, milks, lotions, ointments, spray, liquids. The preparation concerning antioxidant and anti-free radical effects on the skin would preferentially be administered in the form of cream. Assets should be present in a concentration range of 0.1% to 60%. The whole oil or extracts containing the pulp oil antioxidants may be encapsulated after isolation of unsaponifiable fractions, in cyclodextrins or kleptose [alpha, beta, gamma, hydroxypropyl-β-cyclodextrin (HP) type]. This vectorization not only allows protection of the active ingredient, as well as a progressive diffusion within the skin, but also other essential characteristics: the cosmetic touch (by simple massage, the microparticles melt into the skin without leaving traces ), the biodegradability (the constituents of the microparticles are sensitive to the enzymes naturally present in the skin, which progressively degrade them), the safety (the constituents used and their degradation products are nontoxic and perfectly tolerated). The preferred method is to solubilize the HP cyclodextrins in distilled water, (solubilization: 50g / 100 CC: rocket), with stirring, at room temperature (in order to obtain a better viscosity), then add the active form oil whole (ratio 2: 1 oil / HP cyclodextrins) or in unsaponifiable form (ratio 1.5: 1), still at ambient temperature and with stirring, the heterogeneous mixture is left stirring for 4 to 5 hours, at room temperature, then it is precipitated at the end of this time by passing through an ice bucket, then filtered under vacuum, washed with distilled water or ethanol in order to remove the uncomplexed products and finally dried in an oven at 45 ° C. The encapsulated product obtained, in powder form, has a very pale yellow color, while the starting oil is orange-red, and the unsaponifiable product is yellow-orange. The cream formulation of the active ingredients may take the form of an emulsion in which the fatty phase comprises some of the following constituents, for example cetyl palmitate, stearic acid, polyglyceryl-2 dipolyhydroxystearate or glyceryl stearate or Polyglyceryl-3-Diisostearate, butylparaben, coconut glycerides, chitosan, beeswax, tocopherols, Astrocaryum vulgare pulp oil (if the cream consists of whole oil as active), and the aqueous phase would include some of the following constituents by for example: glycerin, distilled water, cyclodextrins (if the active ingredient is in encapsulated form), phenoxyethanol, sodium methyl paraben, isopropyl myristate, mica, titanium dioxide, oxides of zinc or iron. After homogenization of the aqueous phase in the oily phase, would be added the preservatives or perfumes, for example: methyl chloroisothiazolinone, triethanolamine, natural perfume. The presentation of the cream would be in the form of pots or tubes of 50 ml, allowing a hundred applications.

  4. Use in oral or topical form of whole oils of Astrocaryum vulgare in formulations relating to rheumatism-related problems, namely mainly, stiffness and difficulty of mobilization, problems related to an accumulation of oxygen free radicals. The preferential presentation in oral form will combine a mixture of pulp oil and almond oil in a soft gel soft gel capsule. The preferential ratio will be 90-95% of pulp oil and 5-10% of almond oil. This ratio is preferable to other combinations because it has the advantage of not overloading the saturated fatty acid composition, and to be able to mix the two oils, because the almond oil is solid at room temperature. Gelatin capsules (animal, vegetable origin) will comply with the legislation in force, containing no specified risk material, they may be colored (example yellow iron oxide or titanium, indigotine). The dosage will be 2 to 4 capsules daily for 4 weeks minimum, until improvement of the state the capacity of the capsules is 1 ml. The presentation would be in boxes containing 60 capsules. The preferred formulation for topical use would be in ointment form. This formulation comprises an aqueous phase consisting of glycerin and distilled water, an oily phase consisting of beeswax, cocoa butter, camphor, Astrocaryum vulgare oil (mixture of pulp oil and water). almond oil, in a ratio of 10:90 to 90:10), preferential ratio: 60-70% pulp oil and 30-40% almond oil. This ointment would be to spend the evening on the painful areas.

  5. Use of the hygiene formulations in the form of soaps made from Elis guineensis oil, palm kernel oil and Astrocaryum vulgare oil sludge. These Guyana soaps, in the traditional cauldron, by natural drying, are completely natural, without the addition of preservatives or dyes. Several formulations are carried out on the basic composition, with the addition of essential oils, clay, parboiled peel of cassava (manioc.) The preferred formulations are the little sweetie, which includes palm oil, palm kernel oil, natural carotene (Astrocaryum vulgare), clay, essential oils, water with two presentations: tea tree and chocolate clay, with the following actions: exfoliant, antioxidant, treatment of local irritations, nourishing, protective, healing, antifungal. Another preferred formulation is the horsehair glove of the Amazon whose composition is as follows: palm, palm kernel, clay, parboiled couak (cassava), water, essential oils, natural carotene (Astrocaryum) 6 types of basic soap: awara couak, lavender honey flower, cocoa, orange peel, coffee grounds cinnamon, gingembr e their actions are: scrub, antifungal, nourishing, anticellulite, antioxidant. The awara couak, in 200 gr breads, is specific to a pumice scrub for the feet, elbows and knees, the other 5 products are specifically designed for exfoliating and exfoliating worn skin, and to activate the decrease in orange peel and the damage caused by free radicals. 20 25

Claims (8)

- 18 - CLAIMS   1. Oral and topical compositions relating to the antioxidant properties, anti oxygen free radicals, and rheumatic stiffness, characterized in that they include oils and extracts of the fruit of Astrocaryum vulgare.   2. Compositions according to claim 1 characterized in that the pulp oils, almond oils, unsaponifiables, almond powder or cake, used will be derived from Astrocaryum vulgare.   3. Compositions according to claims 1 and 2 characterized in that the oils and protein extracts of the fruit of Astrocaryum vulgare used are used in oral formulations day / night to fight against wrinkles and fine lines due to the harmful effects of the environment and the aging thanks to the antioxidant and anti-free radical properties of these oils.   4. Compositions according to claims 1,2 and 3 characterized in that the oils and fruit extracts of Astrocaryum vulgare are used in a food use, alone or in combination with oil of Elmis guinensis, to give a daily intake of antioxidant compounds and anti free radicals oxygenated to the body.   5. Compositions according to claims 1, 2,3 and 4 characterized in that the oils and fruit extracts of Astrocaryum vulgare are used in topical form, preferably in the form of dermocosmetic creams to be applied to the face, neck and hands and containing the active (oil or unsaponifiable fraction) encapsulated and vectorized via an HP type cyclodextrin, to - 19 fight against the effects of oxygen free radicals by providing natural antioxidants.   6. Compositions according to claims 1, 2, 3,4 and 5, characterized in that the whole oils of the fruits of Astrocaryum vulgare are used in oral form, for example in the form of soft capsules containing fruit pulp oil. 90- 100% Astrocaryum vulgare and Astrocaryum vulgare fruit almond oil at a level of 0-10% or in topical form, for example in the form of 60-70% ointment Astrocaryum vulgare fruit pulp oil and 3040% Astrocaryum vulgare fruit almond oil for the purpose of preventing and treating the accumulation of oxygenated free radicals accumulating in rheumatic joints 7. Compositions according to claims 1, 2, 3, 4,5 and 6, characterized in that the oils and sludges of fruit oils of Astrocaryum vulgare can be used in combination with the oils of El is guineensis and palm kernel oils in topical form represented by hygienic soaps composed of palm oil, palm kernel, clay, manioc steamed barks, water, essential oils, natural carotene (Astrocaryum vulgare) used alone or in addition to the oral and topical formulations described above, to complement the effectiveness of the natural antioxidants provided by the oils and extracts of Astrocaryum vulgare fruits.   8. Compositions according to claims 1, 2, 3, 4, 5,6 and 7 characterized in that they comprise pulp oils and almond oils fruit of Astrocaryum vulgare, unsaponifiables of pulp oil and unsaponifiables of almond oil fruits of Astrocaryum vulgare, protein extracts J   of almond powder of the fruits of Astrocaryum vulgare, in oral form, topical to prevent and fight against the accumulation of free radicals oxygenated thanks to their natural antioxidant effect. IO 15 25 30