FR2785909A1 - Microalgal culture extract with superoxide dismutase-like activity useful for preparing cosmetic, dietetic, pharmaceutical and veterinary products for preventing or treating aging and degeneration - Google Patents

Microalgal culture extract with superoxide dismutase-like activity useful for preparing cosmetic, dietetic, pharmaceutical and veterinary products for preventing or treating aging and degeneration Download PDF

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FR2785909A1
FR2785909A1 FR9814510A FR9814510A FR2785909A1 FR 2785909 A1 FR2785909 A1 FR 2785909A1 FR 9814510 A FR9814510 A FR 9814510A FR 9814510 A FR9814510 A FR 9814510A FR 2785909 A1 FR2785909 A1 FR 2785909A1
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extract
activity
micro
culture medium
sod
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/02—Algae
    • A61K36/04—Rhodophycota or rhodophyta (red algae), e.g. Porphyra
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/03—Algae
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A23L17/60—Edible seaweed
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/02—Algae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9706—Algae
    • A61K8/9717—Rhodophycota or Rhodophyta [red algae], e.g. Porphyra
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/522—Antioxidants; Radical scavengers

Abstract

A microalgal culture extract (I) with a superoxide dismutase (SOD)-like activity of at least 30 U/ml, is new. An Independent claim is also included for a composition (II) comprising a microalgal culture extract that has a SOD-like activity of at least 30 U/ml and/or a sulfated polysaccharide content of at least 1 mg/ml.

Description

Domaine Technique :
L'invention concerne un extrait de micro-algues possédant une activité antiradicalaire due à la présence de SOD (superoxidismutase) like, couplé avec la présence de polysaccharides sulfatés. Ces deux activités sont utilisées seules ou ensemble pour entrer dans des compositions utiles dans les domaines de la pharmacie, de la cosmétique, de la nutrition diététique ou alimentaire et dans la lutte contre les parasites de culture.Technical area :
The invention relates to a microalgae extract having anti-free radical activity due to the presence of SOD (superoxidismutase) like, coupled with the presence of sulfated polysaccharides. These two activities are used alone or together to enter into compositions useful in the fields of pharmacy, cosmetics, dietetic or food nutrition and in the fight against culture pests.

Techniques antérieures :
De nos jours beaucoup de travaux sont relatifs à l'obtention et aux propriétés de substances antiradicalaires. Les chercheurs ont récemment trouvé des sources à partir du monde végétal à cause des substances infectieuses risquant d'tre rencontrées dans les extraits animaux ou humains. Des techniques de clonage sont en étude. Une source moderne est mise en oeuvre avec la culture de micro-algues. L'objectif habituel consiste à extraire la biomasse algale pour trouver des composants intéressants. Ces cultures coûtent cher et sont longues. Le milieu de culture est jeté, ce qui explique une part importante du coût. La présente invention provient des propriétés inattendues que la demanderesse a trouvé non pas dans la biomasse, mais dans son milieu de culture. La biomasse étant préservée et servant à l'obtention connue de composants tels que protéines, lipides, vitamines, sels minéraux. De ce fait, l'invention en valorisant un rejet industriel en plus des propriétés inattendues rend plus économique l'exploitation des micro-algues en question.Previous techniques:
Nowadays a lot of work is related to the obtaining and properties of anti-free radicals. Researchers recently found sources from the plant world because of infectious substances that may be encountered in animal or human extracts. Cloning techniques are being studied. A modern source is used with the culture of micro-algae. The usual goal is to extract algal biomass to find interesting components. These crops are expensive and take a long time. The culture medium is discarded, which explains a large part of the cost. The present invention stems from the unexpected properties that the Applicant has found not in the biomass, but in its culture medium. The biomass being preserved and used to obtain known components such as proteins, lipids, vitamins, mineral salts. Therefore, the invention by valuing an industrial waste in addition to the unexpected properties makes the exploitation of the micro-algae in question more economical.

Description sommaire de l'invention :
L'invention consiste en la préparation d'extrait contenu dans le milieu de culture de micro-algues. Ces cultures sont connues de l'homme de l'art. Mais dans le cas particulier de l'invention, des dispositions sont prises pour augmenter les défenses de l'algue afin d'augmenter la richesse des composants pouvant tre recherchés. La culture est réalisée en conditions contrôlées et de manière axénique, sans produit du type pesticide qui générerait la qualité de 1'extrait objectif de l'invention. De façon particulière après culture proprement dite et selon l'invention, l'algue subit un forçage métabolique. La réaction physiologique de l'algue à une sursaturation en 02 serait en fait la cause de la surproduction des composés antiradicalaires afin de se protéger contre l'oxygène afin d'éviter des dégradations structurales comme les péroxydations lipidiques. La demanderesse a mis au point son invention à partir de cultures choisies dans le groupe des Rhodophycae et notamment le
Porphyridium cruentum (PC). L'extrait obtenu présente deux activités biologiques dues aux superoxydismutases like SODL et aux polysaccharides (PS). L'intért de 1'extrait réside dans la présence simultané des deux activités. Elles interviennent en synergie, la composante
SODL agissant comme anti-inflammatoire facilitant de ce fait la reconstitution tissulaire amenée par les PS, notamment pour des applications cosmétiques. Ces deux effets sont distincts en effet, on peut préparer deux extraits, I'un contenant seulement l'acteur SODL, l'autre contenant les PS. Les mesures d'activités spécifiques montrent qu'elles sont présentes isolément, mais que leur réunion ou utilisation simultanée amplifie chacun des effets antiinflammatoires et régénérants. Brief description of the invention:
The invention consists in the preparation of extract contained in the culture medium of micro-algae. These cultures are known to those skilled in the art. However, in the particular case of the invention, arrangements are made to increase the defenses of the alga in order to increase the richness of the components that can be sought. The culture is carried out under controlled conditions and axenically, without a pesticide type product which would generate the quality of the objective extract of the invention. Particularly after proper culture and according to the invention, the alga undergoes a metabolic forcing. The physiological reaction of the alga to a supersaturation in O 2 would in fact be the cause of the overproduction of the anti-free radical compounds in order to protect themselves against oxygen in order to avoid structural degradations such as lipid peroxidation. The Applicant has developed its invention from cultures chosen from the Rhodophycae group and in particular the
Porphyridium cruentum (PC). The extract obtained has two biological activities due to superoxydismutases like SODL and to polysaccharides (PS). The interest of the extract lies in the simultaneous presence of the two activities. They act in synergy, the component
SODL acting as an anti-inflammatory thereby facilitating the tissue reconstruction brought by the PS, in particular for cosmetic applications. These two effects are distinct in fact, two extracts can be prepared, one containing only the SODL actor, the other containing the PS. The measures of specific activities show that they are present in isolation, but that their combination or simultaneous use amplifies each of the anti-inflammatory and regenerating effects.

Ainsi et selon l'invention on peut concevoir des compositions traitantes contenant en quantité efficace :
1) Un extrait du milieu de culture de micro-algues présentant une activité SOD like d'au moins 30 U/ml.Thus and according to the invention, it is possible to conceive treating compositions containing in an effective amount:
1) An extract from the microalgae culture medium having an SOD like activity of at least 30 U / ml.

2) Un extrait du milieu de culture de micro-algues présentant une teneur en polysaccharides sulfatés au moins égale à 1 mg par ml. 2) An extract from the microalgae culture medium having a content of sulfated polysaccharides at least equal to 1 mg per ml.

3) Un extrait de culture de micro-algues présentant une activité SOD like d'au moins 30 U/ml et une teneur en polysaccharides sulfatés au moins égale à 1 mg par ml. L'extrait peut donc valablement et en quantité efficace entrer dans des compositions à usage cosmétologique, diététique, pharmaceutique destinées à ! a prévention ou au traitement du vieillissement et de la dégénérescence et dans les pathologies liées au stress oxydatif pour l'homme ou l'animal. L'extrait peut intervenir en renforçant les activités anti-oxydantes d'autres extraits végétaux. Ainsi avec des extraits à activité péroxydasique, il a été constaté une augmentation nette de cette activité en leur rajoutant 10 % environ de l'extrait selon l'invention. Cette propriété est intéressante dans la lutte biologique contre les parasites des cultures. 3) A microalgae culture extract having an SOD like activity of at least 30 U / ml and a content of sulphated polysaccharides at least equal to 1 mg per ml. The extract can therefore validly and in effective amount enter into compositions for cosmetic, dietetic or pharmaceutical use intended for! a prevention or treatment of aging and degeneration and in pathologies linked to oxidative stress for humans or animals. The extract can intervene by strengthening the antioxidant activities of other plant extracts. Thus with extracts with peroxidase activity, a net increase in this activity has been observed by adding about 10% of the extract according to the invention to them. This property is interesting in the biological fight against crop pests.

Manières de réaliser l'invention :
L'invention bien que simple dans sa description sera bien comprise à l'aide des exemples qui illustrent ses multiples applications et ses :
Exemple 1. Obtention de 1'extrait à partir de PC
Dans un réacteur de 150 litres, l'algue est cultivée dans un milieu type Conway eau de mer artificielle. L'inoculation est faite par 5 1 de culture à 5 millions de cellules par millilitre.Ways to realize the invention:
The invention, although simple in its description, will be clearly understood with the aid of the examples which illustrate its multiple applications and its:
Example 1. Obtaining the extract from PC
In a 150 liter reactor, the algae is cultivated in a Conway type medium, artificial seawater. The inoculation is carried out by 5 l of culture at 5 million cells per milliliter.

L'agitation et la fourniture de C02 se fait par bullage d'air. L'éclairage est de 50 à 100 micromoles m-2 S-1. Au bout de 12 jours, lorsque la densité cellulaire est optimale, on rajoute 100 mg par litre de KH C03. La gaine en polyéthylène est fermée, et on laisse la culture se poursuivre pendant 2 jours afin de créer un forçage métabolique. Les algues sont séparées par centrifugation du milieu de culture. L'extrait s'obtient alors par filtration à partir du milieu de culture. Celui-ci contient par litre 30 unités de SOD like, mesuré au kit SOD-525 et environ 1 mg de PS par ml. Une moitié de l'extrait est déshydraté par lyophilisation pour préparer des compositions telles que comprimés. L'autre moitié est stérilisée à 120 C pendant 20 minutes et conditionnée dans des flacons stériles, sans que cette opération ne modifie l'activité SOD, ni la teneur en polysaccharides sulfatés.The agitation and supply of C02 is done by air bubbling. The lighting is from 50 to 100 micromoles m-2 S-1. After 12 days, when the cell density is optimal, 100 mg per liter of KH C03 are added. The polyethylene sheath is closed, and the culture is allowed to continue for 2 days in order to create a metabolic forcing. The algae are separated by centrifugation from the culture medium. The extract is then obtained by filtration from the culture medium. This contains 30 liters of SOD like per liter, measured in the SOD-525 kit and approximately 1 mg of PS per ml. Half of the extract is dried by lyophilization to prepare compositions such as tablets. The other half is sterilized at 120 ° C. for 20 minutes and packaged in sterile bottles, without this operation modifying the SOD activity or the content of sulfated polysaccharides.

Exemple 2 : Séparation des activités :
A partir de 10 litres d'extraits de l'exemple 1, on sépare par précipitation solvant (alcool éthylique) la partie SOD like. On obtient ainsi 1'extrait sous deux sous-variantes, 1'une contenant l'activité antiradicalaire non altérée par la séparation, l'autre la présence des polysaccharides. Comme à l'exemple 1 et par déshydratation, 1'extrait et ses variantes peut se conserver liquide ou sous forme de poudre soluble. Example 2: Separation of activities:
From 10 liters of extracts of Example 1, the SOD like part is separated by solvent precipitation (ethyl alcohol). The extract is thus obtained in two sub-variants, one containing the anti-free radical activity not altered by the separation, the other the presence of the polysaccharides. As in Example 1 and by dehydration, the extract and its variants can be kept liquid or in the form of soluble powder.

Exempt 3 ;
Crème anti-âge selon la formulation :
PEG-8 BEESWAX 8
OCTYDODECYL MYRISTATE 10
ISOSTEARYL ISOSTERATE 10
EXTRAIT SELON EX 1 10
SODIUM HYDROXIDE 0,4
ETHOXYDIGLYCOL 5
EAU PARFUM, CONSERVATEUR QS
Exemple 4 :
Objectivation in vitro de la crème anti-âge. On constate une prolifération de fibroplastes, mme en présence d'agents anti-prolifératifs qui indiquerait un effet trophique et donc pro-reconstituant.Free 3;
Anti-aging cream according to the formulation:
PEG-8 BEESWAX 8
OCTYDODECYL MYRISTATE 10
ISOSTEARYL ISOSTERATE 10
EXTRACT ACCORDING TO EX 1 10
SODIUM HYDROXIDE 0.4
ETHOXYDIGLYCOL 5
PERFUMED WATER, QS PRESERVATIVE
Example 4:
In vitro objectification of anti-aging cream. There is a proliferation of fibroplasts, even in the presence of anti-proliferative agents which would indicate a trophic effect and therefore pro-reconstituting.

Exemple 5 : Composition diététique anti-stress oxydati
On réalise des gélules de 250 mg de poudre selon l'exemple 1, destinées à la nutraceutique ou à la parapharmacie. Sur rat, on mesure dans le plasma l'augmentation des substances antiradicalaires induite par le gavage journalier de 5 mg de poudre.EXAMPLE 5 Dietetic Anti-Oxidative Stress Composition
250 mg powder capsules are produced according to Example 1, intended for nutraceuticals or for the drugstore. In rats, the increase in anti-free radicals induced by daily force-feeding of 5 mg of powder is measured in plasma.

Exemple 6 : Brumatiseur adoucissant pour peau fragilisée
Le liquide de 1'exemple 1 est placé dans un flacon propulseur à l'azote.EXAMPLE 6 Softening Brumatizer for Fragile Skin
The liquid of Example 1 is placed in a nitrogen propellant bottle.

Exemple 7 : Préparation d'un collyre anti-inflammatoire
Selon la formulation suivante :
Gluconate de chlorhexidine 0,005 g
Inosine phosphate disodique dihydrate 0,5 g
Extrait liquide selon exemple 1 q. s. q 100 ml
Exemple 8 : Extrait anti-oxydant dans un produit alimentaire
On rajoute à un concentré à 50 brix de tomates 0,1 % de la poudre obtenue à 1'exemple 1. EXAMPLE 7 Preparation of an Anti-Inflammatory Eye Drop
According to the following formulation:
Chlorhexidine gluconate 0.005 g
Inosine disodium phosphate dihydrate 0.5 g
Liquid extract according to example 1 qs q 100 ml
Example 8: Antioxidant Extract in a Food Product
0.1% of the powder obtained in Example 1 is added to a concentrate of 50 brix of tomatoes.

Exemple 9 : Extrait pour lutter contre les parasites des cultures
On rajoute 10 % de 1'extrait liquide de 1'exemple 1 à un extrait végétal ayant une activité péroxydasique de 460 nKat/ml. Après une journée de stockage à température ambiante l'activité est de 800nKat/ml au lieu de 300 nKat/ml sans rajout.EXAMPLE 9 Extract for Controlling Pests of Cultures
10% of the liquid extract of Example 1 is added to a plant extract having a peroxidase activity of 460 nKat / ml. After a day of storage at room temperature the activity is 800nKat / ml instead of 300 nKat / ml without addition.

Au bout de 4 jours on mesure les activités :
L'extrait péroxydasique seul : 207 nKat/ml
Avec addition de 10 % 501 Kat/ml After 4 days we measure the activities:
Peroxidase extract alone: 207 nKat / ml
With addition of 10% 501 Kat / ml

Claims (13)

1. Extrait de milieu de culture de micro-algues caractérisé en ce qu'il présente une activité SOD like d'au moins 30 unités par ml. 1. Extract of micro-algae culture medium, characterized in that it has an SOD like activity of at least 30 units per ml. REVENDICATIONS 2. Extrait de culture de micro-algues présentant une teneur en polysaccharides sulfatés au moins égale à 1 mg par millilitre. 2. Microalgae culture extract with a content of sulfated polysaccharides at least equal to 1 mg per milliliter. 3. Extrait selon les revendications 1 et 2 obtenu par forçage métabolique en fin de ladite culture dans des conditions induisant une surproduction de composés antiradicalaires. 3. Extract according to claims 1 and 2 obtained by metabolic forcing at the end of said culture under conditions inducing an overproduction of anti-free radical compounds. 4. Extrait selon les revendications 1 à 3 caractérisé en ce que la micro-algue est choisie parmi le groupe des Rhodophycae et notamment le Porphyridium cruentum. 4. Extract according to claims 1 to 3 characterized in that the micro-alga is chosen from the group of Rhodophycae and in particular Porphyridium cruentum. 5. Extrait selon les revendications 1 à 4 caractérisé en ce que les composés responsables de l'activité SOD like ont un poids moléculaire inférieur à 30 000. 5. Extract according to claims 1 to 4 characterized in that the compounds responsible for the SOD like activity have a molecular weight of less than 30,000. 6. Extrait selon l'une quelconque des revendications 1 à 5 caractérisé en ce qu'il est thermostable et supporte une température au moins égale à 120 C pendant au moins 20 minutes. 6. Extract according to any one of claims 1 to 5 characterized in that it is thermostable and withstands a temperature at least equal to 120 C for at least 20 minutes. 7. Composition traitante choisie parmi le groupe comprenant en quantité :  7. Treatment composition chosen from the group comprising in quantity: a) Un extrait du milieu de culture de micro-algues présentant une activité SOD like d'au moins 30 U/ml. a) An extract from the micro-algae culture medium having an SOD like activity of at least 30 U / ml. b) Un extrait du milieu de culture de micro-algues présentant une teneur en polysaccharides sulfatés au moins égale à lmg par ml. b) An extract from the microalgae culture medium having a content of sulfated polysaccharides at least equal to 1 mg per ml. c) Un extrait du milieu de micro-algues présentant une activité SOD like d'au moins 30 U/ml et une teneur en polysaccharides sulfatés au moins égale à 1 mg/ml. c) An extract from the micro-algae medium having an SOD like activity of at least 30 U / ml and a content of sulphated polysaccharides at least equal to 1 mg / ml. 8. Composition selon la revendication 7 caractérisée en ce que 1'extrait de milieu de culture subit un forçage métabolique en fin de culture dans des conditions induisant une surproduction des composés antiradicalaires. 8. Composition according to claim 7 characterized in that the culture medium extract undergoes a metabolic forcing at the end of culture under conditions inducing an overproduction of the anti-free radical compounds. 9. Composition contenant 1'extrait selon les revendications 7 et 8 caractérisée en ce que la micro-algue est le Porphyridium cruentum. 9. Composition containing the extract according to claims 7 and 8 characterized in that the micro-alga is Porphyridium cruentum. 10. Utilisation d'un extrait de milieu de culture de micro-algues présentant une activité SOD like d'au moins 30 U/ml et une teneur en polysaccharides sulfatés au moins égale à lmg/ml en quantité efficace pour la préparation de compositions à usage cosmétologique, diététique, pharmaceutique destinées à la prévention ou au traitement du vieillissement et de la dégénérescence. 10. Use of an extract of microalgae culture medium having an SOD like activity of at least 30 U / ml and a content of sulphated polysaccharides at least equal to 1 mg / ml in an amount effective for the preparation of compositions with cosmetic, dietetic, pharmaceutical use intended for the prevention or treatment of aging and degeneration. 11. Utilisation d'un extrait de milieu de culture de micro-algues selon la revendication 10 dans les pathologies liées au stress oxydatif.  11. Use of an extract of micro-algae culture medium according to claim 10 in pathologies linked to oxidative stress. 12. Utilisation d'un extrait de culture de micro-algues selon la revendication 10 dans la lutte contre les parasites de cultures, en l'associant à d'autres extraits végétaux à activité antioxydante. 12. Use of a microalgae culture extract according to claim 10 in the fight against crop pests, by combining it with other plant extracts with antioxidant activity. 13. Utilisation d'un extrait de milieu de culture de micro-algues selon les revendications 10 et 11 pour l'homme ou l'animal.  13. Use of an extract of micro-algae culture medium according to claims 10 and 11 for humans or animals.
FR9814510A 1998-11-16 1998-11-16 Microalgal culture extract with superoxide dismutase-like activity useful for preparing cosmetic, dietetic, pharmaceutical and veterinary products for preventing or treating aging and degeneration Pending FR2785909A1 (en)

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FR9814510A FR2785909A1 (en) 1998-11-16 1998-11-16 Microalgal culture extract with superoxide dismutase-like activity useful for preparing cosmetic, dietetic, pharmaceutical and veterinary products for preventing or treating aging and degeneration
FR9907372A FR2785910B1 (en) 1998-11-16 1999-06-08 PROCESS FOR OBTAINING FROM THE MICROALGAE CULTURE MEDIUM A THERMOSTABLE EXTRACT HAVING ANTI-OXIDANT AND PRO-HEALING ACTIVITY

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EP1307212A2 (en) * 2000-07-31 2003-05-07 World Resources Corporation Plant derived antioxidants
FR2899065A1 (en) * 2006-03-29 2007-10-05 Alain Dreyer Brevets Et Dev So Composition useful e.g. as veterinary medicament with antioxidant action in animals, comprises food medium for animal feed and microgranules adsorbed on and/or mixed with mineral support/vegetable base
FR2999386A1 (en) * 2012-12-17 2014-06-20 Bionov COMPOSITION FOR STIMULATING THE VITALITY OF PLANTS
US9119870B2 (en) * 2005-12-08 2015-09-01 Ben Gurion University Of The Negev Research And Development Authority Viscosupplementation with algal polysaccharides in the treatment of arthritis
EP3091069A1 (en) * 2015-05-06 2016-11-09 Fitoplancton Marino S.L. Method for obtaining a biomass of a microalga of the species tetraselmis chuii enriched in superoxide dismutase (sod)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1307212A2 (en) * 2000-07-31 2003-05-07 World Resources Corporation Plant derived antioxidants
EP1307212A4 (en) * 2000-07-31 2004-12-08 World Resources Corp Plant derived antioxidants
US9119870B2 (en) * 2005-12-08 2015-09-01 Ben Gurion University Of The Negev Research And Development Authority Viscosupplementation with algal polysaccharides in the treatment of arthritis
FR2899065A1 (en) * 2006-03-29 2007-10-05 Alain Dreyer Brevets Et Dev So Composition useful e.g. as veterinary medicament with antioxidant action in animals, comprises food medium for animal feed and microgranules adsorbed on and/or mixed with mineral support/vegetable base
FR2999386A1 (en) * 2012-12-17 2014-06-20 Bionov COMPOSITION FOR STIMULATING THE VITALITY OF PLANTS
WO2014095900A1 (en) * 2012-12-17 2014-06-26 Bionov Composition for stimulating plant vitality
US9854808B2 (en) 2012-12-17 2018-01-02 Bionov Composition for stimulating plant vitality
EP3091069A1 (en) * 2015-05-06 2016-11-09 Fitoplancton Marino S.L. Method for obtaining a biomass of a microalga of the species tetraselmis chuii enriched in superoxide dismutase (sod)
WO2016177853A1 (en) * 2015-05-06 2016-11-10 Fitoplancton Marino, S.L Method for obtaining a biomass of a microalga of the species tetraselmis chuii enriched in superoxide dismutase (sod)
US11473065B2 (en) 2015-05-06 2022-10-18 Fitoplancton Marino, S.L. Method for obtaining a biomass of a microalga of the species Tetraselmis chuii enriched in superoxide dismutase (SOD)

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