FR2899065A1 - Composition useful e.g. as veterinary medicament with antioxidant action in animals, comprises food medium for animal feed and microgranules adsorbed on and/or mixed with mineral support/vegetable base - Google Patents

Abstract

Composition comprises a food medium for animal feed and microgranules adsorbed on and/or mixed with a mineral support or a vegetable base, where the microgranule comprises an active vegetable extract containing super oxide dismutase and the extract is microencapsulated in a fat based liposoluble agent. Independent claims are included for: (1) the preparation of the composition comprising melting the liposoluble agent to obtain a liquid, depositing the liquid on the active vegetable extract containing the superoxide dismutase to obtain a microgranules, adsorbing and/or mixing the microgranules on and/or with a mineral support, adsorbing and/or mixing the adsorbed and/or mixed microgranules on and/or with a vegetable base, and mixing the adsorbed and/or mixed microgranules with the food medium for animal feed; (2) a raw material for animal feed and/or veterinary purpose comprising the composition; (3) a composition for veterinary purpose comprising the composition or the raw material and an excipient suitable for oral use as animal feed; (4) a premixing of a food additive for animals comprising the composition and a food additive such as growth promotors, bacterial floras, vitamins and other antioxidants and/or trace elements for animal; and (5) an animal feed comprising the composition, the premix or the raw material. ACTIVITY : Antiinflammatory; Tranquilizer; Gynecological; Immunosuppressive. MECHANISM OF ACTION : None given.

Description

The present invention therefore relates to a composition comprising a support

  feedstock and microgranules adsorbed onto and / or mixed with a mineral carrier and adsorbed onto and / or mixed with a plant carrier, said microgranules being an active plant extract containing superoxide dismutase, said extract being microencapsulated in a liposoluble fat-based agent.

  Under normal conditions, an animal's body continuously produces reactive oxygen derivatives, oxidizing molecules, which are rapidly destroyed or neutralized by anti-oxidant defenses. This occurs in general, during any biochemical reaction involving molecular oxygen.

  Initially oxygen free radicals are produced, also called primary free radicals (O 2, H 2 O 2, OH). The defense systems responsible for eliminating these primary free radicals consist of anti-oxidant enzymes (SOD, Catalan, peroxidases) called primary antioxidants, acting as shown in the diagram below: SOD 02 ù - 002 The primary free radicals not removed by these enzymes cause the formation of secondary free radicals (ROO), capable of chain oxidation reaction. Their toxicity is important for cell structures which can lead to many pathologies. To neutralize these secondary free radicals the organism brings into play a second complementary line of defense which are external molecules brought by the food (Vit E, C, A, polyphenols), which one also calls the scavengers or secondary antioxidants, acting as shown in Figure 1. Thus the primary antioxidants prevent the formation of oxygen free radicals while secondary antioxidants participate in slowing chain oxidation reactions thus limiting the cell damage. Oxidative stress is the result of an imbalance between the production and destruction of free radicals. Under normal physiological conditions the organism of an animal continuously produces free radicals. This basic production is necessary for the proper functioning of the organism. It is the intensity, duration and location of their production that will determine the effects of free radicals. Thus, a moderate and transient increase in their production corresponds to a defense mechanism of the cell, allowing it, for example, to destroy cancer cells or pathogenic microorganisms.

  On the other hand, a moderate but recurrent or chronic increase in free radicals disturbs the balance between their production and their destruction on a continuous basis. This causes cellular and organic functional imbalances. This is called oxidative stress. This is for example what happens during chronic inflammations: osteoarthritis, tendonitis, mastitis causing the presence of cells in the milk, nesting during reproduction or during periods of stress: competitions for sports animals: horses or dog, before, during, and after competitions; start-up periods in breeding: chicks, nurseries, alvelinage, transitions: farrowing, spawning, weaning, change of basin, transport; vaccinations; intensive feeding; successful productions; intense and prolonged efforts: running, working or walking; or aging. In addition, an increase large enough to irreversibly alter vital cellular processes triggers apoptosis (cell death by implosion, without spilling the cellular content): it is a major mechanism of the organization of the immune system, particularly at the level of the cells. T and B lymphocytes Excessive activation of apoptosis triggers neurodegenerative, neurological, and cardiac diseases, whereas its insufficiency leads to the development of malignant tumors. Finally, a massive production of free radicals leads to cell necrosis (cell death by explosion with spilling of the entire cellular content). It is very quickly what happens in acute inflammations, whether microbial, viral, physical, chemical or ionic. Oxidative stress causes a weakening of the antioxidant defenses by: - Decrease of the tissue contents in compounds scavengers of free radicals (ubiquinol, glutathione, vitamin C, carotenoids, vitamins A, vitamins E) - decrease in the activity of the antioxidant enzymes SOD, catalase, peroxidases). This translates directly into the animals' vitality, performance and disease resistance.

  To contain this phenomenon of oxidative stress and thus help the cell and the body to eliminate free radicals, it is therefore advantageous to reinforce the pool of primary antioxidants such as SOD by external input as shown in the diagram below:

  L O 2 O 2 • 1 H 2 O 2 • ter ol-r. ___ ROO

  Thus, the search for antioxidant substances, in particular containing SOD, has been the subject of constant interest both in the field of cosmetology and in the food industry. or medicine, especially for administration in humans. Fe 'Fe "T H2O + 02 Catalase Peroxidase The patent FR 2 287 899 for example describes the application in cosmetology of superoxide dismutase enzymes and in particular the use of these enzymes in the preparation of cosmetic compositions for the care of the skin and SOD has therefore been used in pathologies induced by free radicals, especially in the case of chronic inflammations, for example in the treatment of Crohn's disease (Emerit et al., 1991, Free Rad Res. Comms., 12-13, 563-569) or in the case of radiation-induced fibrosis (Delanian et al., 1994, Radiotherapy and Oncology, 32, 12-20) These superoxide dismutases are notably extracted from beef erythrocytes. (Markovitz, J. Biol. (Hem., 234: 40, 1959), Escherichia coli (Keele and Fridovitch, J. Biol., 245, pp. 6176, 1970) and marine bacterial strains (FR Patents 2 225 443 and FR 2 240 277), but for reasons related in particular to infectious substances such as Although they may contain these materials, there is a tendency nowadays to replace them with substances from the plant kingdom considered to be healthier. These antioxidant enzymes are indeed present in varying amounts in certain plants. Thus, the patent application FR 2,716,884 describes a protein extract of Cucumis melo having an improved enzymatic activity superoxide dismutase, their method of preparation and their use in pharmaceutical or cosmetic compositions for external topical use. However, such a protein extract presents difficulties of use, the enzymes it contains (superoxide dismutase in particular) being inactivated in the presence of external aggressions (UV in particular) or during absorption in oral formulations (Gastric acid). It is then necessary to develop specific formulations that provide this protection to optimize the action of antioxidant actives. To increase the plasma half-life of free SOD (Cu / Zn, Fe or Mn) weasels, various modified forms for parenteral administration have been proposed; mention may be made of SOD conjugated with polyethylene glycol (SOD-PEG), heparin-conjugated SOD (SOD-Heparin), SOD-conjugated SOD (SOD-albumin), SOD polymers or copolymers and SODs liposomal.

  However, these different SOD have the major disadvantage of being very little absorbed when they are administered orally. The patent application FR 2 729 296 also describes a particular pharmaceutical composition well suited to the oral administration of superoxide dismutase comprising essentially in combination a superoxide dismutase and a compound selected from the group consisting of ceramides, prolamines and polymeric films based on said prolamines. This coating is insufficient to meet the physiological specifications of certain animal species and in particular herbivores including ruminants and coecotrophs in particular. In fact, these animals digest cereals rapidly, including wheat gluten, thus depriving this type of coating of part of its effectiveness. The patent application FR 2 822 381 describes active plant extracts, in particular containing superoxide dismutase and especially a protein extract of cucumis melo, coated and / or microencapsulated in a fat-soluble agent based on fat. However, such a composition is not adapted to the specific constraints of certain technologies applied in animal feed industries, in particular is not sufficiently resistant to heating during granulation or extrusion, nor to pressure constraints. in the granulation systems.

  The inventors have surprisingly discovered that when an active plant extract containing superoxide dismutase (SOD) was microencapsulated in a liposoluble fat-based agent and then adsorbed on a mineral support and then on a vegetable support and finally mixed with a support for animal feed, this composition could be used orally for the feeding of monogastric animals as well as polygastrics and coecotrophs, and its active ingredients, in particular SOD, were protected from the constraints experienced during the processes used. The present invention thus relates to a composition comprising a feed carrier for animal feed and microgranules adsorbed onto and / or mixed with a mineral carrier and adsorbed onto and / or mixed with a carrier v getal, said microgranules being constituted by an active plant extract containing superoxide dismutase, said extract being microencapsulated in a lipid soluble agent based on fat.

  Thus, the composition according to the present invention comprises a food carrier for food and microgranules. These microgranules consist of an active plant extract containing superoxide dismutase, this extract being encapsulated in a liposoluble fat-based agent. These microgranules have a particle size of about 200 m. Moreover, these microgranules are adsorbed onto and / or mixed with a mineral support, these adsorbed and / or mixed microgranules also being adsorbed onto and / or mixed with a vegetable support. Advantageously, these microgranules are adsorbed on a mineral support and advantageously the adsorbed microgranules are also adsorbed on a plant support.

  For the purposes of the present invention, the term "active plant extract containing superoxide dismutase" means any extract obtained from plants having enzymatic superoxide dismutase activity. Advantageously it is a protein extract of pure cucumis melo, in particular powder, preferably lyophilized. Advantageously, this extract is water-soluble. Even more advantageously, cucumis melo is a descendant of the 95LS444 cell line (the seeds of which have been deposited according to the Budapest Treaty in the NCIMB Collection (National Collection of Industrial and Marine Bacteria ABERDEEN AB2 IRY (Scotland - UK) 23 St Machar Drive) on July 19, 1990 under number 40310) or one of the hybrid lines from 95LS444, and more particularly commercial varieties such as Vauclusien Clipper and Supporter.

  In a particular embodiment, the superoxide dismutase enzymatic activity of the plant extract is at least equal to 5 enzymatic units per mg of extract, advantageously at least 50 enzymatic units per mg of extract (measured by the method). OBERLEY AND SPITZ - 1985).

  The plant extract can be obtained by methods that are well known to those skilled in the art, in particular by the method described in patent application FR 2 716 884. Advantageously, the plant extract according to the present invention may furthermore have a Catalase enzymatic activity. It may also include coenzyme Q10, vitamins, lipoic acid, glutathione, mineral elements present in the plant, such as potassium, magnesium, calcium and selenium etc.

  For the purposes of the present invention, liposoluble fat-based agent is understood to mean any agent containing at least one fat and having an equilibrium index between the hydrophilic part and the lipophilic part of the molecule (HLB) of less than 10%. Advantageously, the liposoluble agent according to the present invention has a melting point of from about 40 to about 80 C, even more preferably from about 55 to about 60 C. In a particular embodiment, the fat-soluble agent according to the present invention is of plant origin. Advantageously, it is chosen from the group consisting of hydrogenated oils; palm oil or palm fruit heart; hydrogenated seeds; stearates, in particular chosen from stearins, stearic acid and its derivatives; waxes; monodiglycerides of fatty acid; C14-C20 saturated fatty acid triglycerides and mixtures thereof. Advantageously, the liposoluble agent according to the present invention is hydrogenated oil based on vegetable oil, in particular chosen from the group consisting of hydrogenated coconut oil, hydrogenated palm oil, oil Hydrogenated soybean or hydrogenated rapeseed oil and their mixture. Even more advantageously, it is hydrogenated palm oil.

  It is also possible for the fat-soluble agent to contain water-soluble substances such as sugars or polyols. In a particular embodiment, the plant extract according to the invention is present at a concentration of between 1 and 90% by weight relative to the total weight of the microgranule (excluding plant and mineral supports), advantageously between 20 and 40%. by weight relative to the total weight of the microgranule. In another embodiment according to the present invention, the microgranules (excluding plant and mineral supports) according to the present invention are present at a concentration of between 10 and 30% by weight relative to the total weight of the composition according to the present invention.

  For the purposes of the present invention, the term mineral carrier is understood to mean any raw material of mineral origin authorized for animal feed (according to Decree No. 86-1037 of September 15, 1986).

  Advantageously, it is chosen from the group consisting of: calcium carbonate, advantageously obtained by milling sources of calcium carbonate, such as limestone, oyster shell or mold or by precipitation from an acidic solution ; calcium carbonate and magnesium, advantageously it is a natural mixture of calcium carbonate and magnesium; marine calcareous algae (maerl), advantageously obtained from crushed limed seaweed or transformed into granules; magnesium oxide; magnesium sulfate - dicalcium phosphate, advantageously it is calcium hydrogen phosphate precipitated from bone or inorganic materials (CaHPO4.xH2O); monobicalcium phosphate, advantageously it is a product composed of equal amounts of dicalcium phosphate and monocalcium phosphate; the defluorinated natural phosphate, advantageously obtained by grinding purified and suitably defluorinated natural phosphates; - degelatinized bone meal, advantageously obtained from degreased bone, degelatinized, sterilized or ground; - monocalcium phosphate; - calcium and magnesium phosphate; monoammonium phosphate; sodium chloride, advantageously obtained by grinding natural sources of sodium chloride, such as rock salt and marine sediments; magnesium propionate; magnesium phosphate; sodium phosphate, calcium phosphate and magnesium phosphate; - monosodium phosphate; - sodium bicarbonate; and their mixtures. Advantageously, it is a source of calcium and / or carbonate.

  Advantageously, the inorganic support is in solid form, advantageously in powder form, even more advantageously in micronized form. Advantageously, the inorganic support represents between 0.1 and 50% by weight of the total weight of the composition, advantageously between 20 and 40% by weight of the total weight of the composition.

  By plant support is meant in the sense of the present invention any raw material of plant origin authorized for animal feed (according to Decree No. 86-1037 of September 15, 1986). Advantageously, the plant carrier according to the present invention is chosen from the group consisting of: cereal grains, their authorized products and by-products in animal feed; - oil seeds or oleaginous fruits, their products and by-products authorized for animal feed; 30 - Legume seeds, their products and by-products authorized for animal feed; - tubers and roots, their products and by-products authorized for animal feed; - other seeds and fruits, their authorized products and by-products in animal feed; - fodder, including roughage, authorized for animal feed; - other plants, their products and by-products authorized for animal feed; and their mixture.

  In particular, cereal grains, their products and by-products authorized in animal feed are chosen from: oats; oatmeal; oat shelled oats (a by-product obtained from the processing of oats previously cleaned and dehulled into groats and flours, consisting mainly of oat bran and some of the endosperm); Oatmeal (a by-product obtained from the processing of oats previously cleaned into oatmeal, consisting mainly of outer shells and oat bran); barley; barley products (a by-product obtained from the processing of barley previously cleaned and peeled into pearled barley, semolina or flour); barley protein (a dried by-product of barley starch, consisting mainly of protein obtained during the separation of starch); broken rice; rice bran (brown rice feed flour: a by-product obtained during the first polishing of the cargo rice, consisting of silver film, particles of the aleurone layer, endosperm and germs); rice bran (white rice feed flour: a by-product obtained during the second polishing of the cargo rice, consisting mainly of endosperm particles, the aleurone layer and germs); fodder meal from parboiled rice (a by-product obtained by polishing parboiled rice rice) consisting mainly of silver film, particles of the aleurone layer, endosperm, seeds and varying amounts of calcium carbonate from the manufacturing process); ground fed rice (the product obtained by the milling of fodder rice consisting either of unripe or chalky green grains, obtained by sieving, during the processing of the husked rice, or by grains of rice normally constituted, shelled, spotted or yellow); rice sprout pressure cake (a by-product of pressure-oil milling from rice sprouts to which parts of the endosperm and testa still adhere); rice seed extract (an oil-processing by-product obtained from extraction of rice seeds to which parts of the endosperm and testa still adhere); rice starch; millet; the Rye ; low rye flour (a by-product of the manufacture of flour from cleaned rye consisting mainly of endosperm particles and also fine fragments of husks and some grain debris); remolding of rye (a by-product of the manufacture of flour obtained from cleaned rye consists mainly of fragments of husks and also of particles of grain free from endosperm to a lesser extent than rye bran ); rye bran (a by-product of the manufacture of flour from cleaned rye, consisting mainly of fragments of husks and also of grain particles freed of most of the endosperm); sorghum; wheat ; wheat flour (a by-product obtained from the manufacture of low wheat flour from previously separated wheat or shelled spelled wheat grains consisting mainly of endosperm particles and also fine fragments of husks and some grain debris); the remolding of wheat (a by-product obtained from the manufacture of flour from previously cleaned wheat grains or spelled spelled wheat, consisting mainly of fragments of husks and also of grain particles which have been removed less endosperm only in wheat bran); wheat bran (a by-product obtained from the manufacture of flour from previously cleaned wheat grains or spelled spelled wheat, consisting mainly of fragments of husks and also of grain particles, the bulk of which endosperm has been removed); wheat germ (a by-product obtained in the manufacture of flour consisting essentially of wheat germ, whether flattened or not, to which endosperm and envelope fragments may still adhere); wheat gluten (a dried by-product of wheat starch, consisting mainly of gluten obtained during the separation of starch); Wheat gluten feed (a by-product obtained in the manufacture of starch and wheat gluten, consisting of bran, of which the germ has been partially removed or not, and gluten, to which very small quantities broken wheat resulting from grain screening and very small amounts of starch hydrolysis residues can be added); wheat starch; pregelatinized wheat starch (product composed of wheat starch, largely pregelatinized by heat treatment); spelled; triticale; the corn ; corn meal (a by-product obtained from the manufacture of flour or maize meal) consisting mainly of fragments of husks and grain particles which have been removed less endosperm than in the bran But) ; corn bran (a by-product obtained in the manufacture of flour or maize meal consisting mainly of husks and some fragments of maize sprouts and endosperm fragments); corn sprouts (a by-product of oil mills obtained by the pressure of dry or wet maize sprouts to which parts of the endosperm and testa can still adhere); corn seed extract (oil by-product obtained by extraction of dry or wet maize sprouts to which parts of the endosperm or testa may still adhere); the advantageously micronised corn gluten feed (a by-product of corn starch obtained by the wet process) consisting of bran, gluten and the addition of maize screening residues in a proportion not exceeding 15% by weight and / or residues from maize quenching water used for the production of alcohol or other derivatives of maize starch The product may further contain residues from the extraction of corn germ oil also obtained by the wet process); corn gluten (a dried by-product of corn starch, consisting mainly of gluten resulting from the separation of starch); corn starch; pregelatinized maize starch (or extruded corn starch: product consisting of corn starch, largely pregelatinized by heat treatment); rootlets of malt (malting by-product consisting essentially of dried rootlets of sprouted cereals); dried brewer's dough (brewery by-product obtained by drying malted or unmalted grain residues and other starch products); dried distillery dried fruit (a by-product of the distillation of alcohol obtained by drying fermented grain residues); the distillers' distillers' dark (or distillers') droughts and distillery by-products obtained by drying the fermented grain residues to which a part of the syrup or evaporated residues of the quenching waters have been added) In particular, the seeds or oleaginous fruits, their products and by-products authorized in animal feed are chosen among: the pressure cake of partially shelled peanuts (by-product of oil mill obtained by pressure of partially shelled peanut seeds); partially husked peanut cake (a by-product of an oil mill obtained by extraction of partially shelled peanut seeds (maximum crude fiber content: 16% of the dry matter)); shelled peanut pressure cake (a by-product of oil milling obtained by pressing shelled peanut seeds); shelled peanut cake (a by-product of oil mills obtained by extraction of shelled peanut seeds); rapeseeds; rapeseed oil cake (by-product of rapeseed oil mill); rapeseed meal (a by-product of oil mills obtained by rapeseed extraction); rapeseed (a by-product obtained from the dehulling of rapeseeds); Soybean seed extract cake (a by-product of an oil mill obtained by extraction of partially-shelled safflower seeds); coconut pressure cake (a by-product of oil manufacture obtained by pressing the dried almond (endosperm) and the shell (integument) of the coconut); coconut oil cake (a by-product of oil manufacture obtained by extraction of dried almond (endosperm) and shell (integument) from coconut nuts); palm kernel pressure cake (a by-product of oil mills obtained by pressing palm nuts as far as possible from their woody shells); palm kernel oilcake (a by-product of oil mills obtained by extracting palm nuts as much as possible from their woody husks); cooked soybeans; cooked soybean meal (a by-product of oil mills obtained by extraction of soybeans that have undergone appropriate heat treatment); cooked dehulled soybean meal (a by-product of oil mills obtained by extraction of soya beans, dehulled and heat-treated); soy protein concentrate (the product obtained by extraction of re-extracted soybean seeds that have been re-extracted to reduce the level of non-nitrogenous extractives); vegetable oil; dandruff (soya beans); cotton seeds; the meal for the extraction of partially decorticated cottonseed (a by-product of an oil mill obtained by extraction of cottonseeded and partially peeled cottonseed); cotton cottonseed cake (by-product of oil mills obtained by pressing cottonseed removed from their fibers); press cakes from niger seeds (a by-product of oil manufacture obtained by pressing niger seeds); sunflower seeds; sunflower seed cake (a by-product of oil manufacture obtained by sunflower seed extraction); partially decorticated sunflower seed cake (a by-product of oil mills obtained by extracting sunflower seeds partially freed of their shells); flax seeds ; flax seed pressure cake (by-product of oil mill obtained by flax seed pressure); flaxseed meal (a by-product of oil mills obtained by extraction of flaxseed); olive cake (olives) (an oil-mill by-product obtained by extracting pressed olives as much as possible free of kernel debris); sesame seed pressure cake (sesame seed oil by-product); partially decorticated cocoa seed cake (a by-product of oil mills obtained by extracting dried and roasted cocoa beans partially freed from their hulls); cocoa hulls (coats of dried and roasted cocoa beans) and their mixture. In particular legume seeds, their products and by-products authorized in animal feed are chosen from: chickpeas; guar extraction flour; the ers; the gese; lenses; sweet lupine; cooked beans; the peas ; pea issues (pea meal: a by-product of pea flour production consisting mainly of endosperm particles and, to a lesser extent, dandruff); pea bran (a by-product obtained when grinding peas, consisting mainly of pellicles from peanut removal and pea cleaning); beans and fababeans; the jarosse; vetch and their mixture. In particular, tubers and roots, their products and by-products authorized in animal feed are chosen among: beet pulp (sugar); beet molasses (sugar) (by-product consisting of the syrupy residue collected during the manufacture or refining of sugar beet sugar); sugared (sugar) beet pulp (a by-product of the manufacture of sugar consisting of dried sugar beet pulp to which molasses has been added); beet vinasse (sugar) (by-product obtained after fermentation of sugar beet molasses during the production of alcohol, yeasts, citric acid or other organic substances); sugar (beet); sweet potato ; the manioc ; pregelatinized cassava starch (starch obtained from cassava roots, highly pregelatinized by the application of a suitable heat treatment); potato pulp; potato starch; potato protein (a starch by-product consisting essentially of protein substances resulting from the separation of starch); potato flakes (a product obtained by rotary drying potatoes that have been washed, peeled or unpeeled and parboiled); concentrated potato juice (potato starch residue from which some of the protein and water have been extracted); swollen potato starch (a product consisting of largely pregelatinized potato starch) and their mixture. In particular the other seeds and fruits, their products and by-products authorized in animal feed are chosen among: the pods of locust beans; citrus pulp; fruit pomace (a by-product obtained by pressing in the manufacture of pome or stone fruit juice); tomato pulp; grape seed cake (a by-product obtained when extracting oil from grape seeds); grape pulp (grape marc, dried quickly after the extraction of alcohol and as much as possible freeing of grape seeds and grape seeds); grape seeds (pips extracted from grape marc, not deoiled) and their mixture.

  In particular fodder, including roughage, authorized in animal feed are selected among: alfalfa meal; alfalfa marc (a dried by-product obtained after separation of alfalfa juice by mechanical extraction); alfalfa protein concentrate (product obtained by artificial drying of alfalfa press juice fractions, centrifuged and heat-treated to precipitate the proteins); clover flour; grass meal (product obtained by drying and milling young fodder plants); cereal straw; processed cereal straw and their mixture. In particular the other plants, their products and by-products authorized in animal feed are chosen from: sugar cane molasses (by-product consisting of the syrupy residue collected during the manufacture or refining of sugar from the cane. sugar); sugar carmine vinasse (by-product obtained after fermentation of cane sugar molasses during the production of alcohols, yeasts, citric acid or other organic substances); sugar (cane); seaweed meal and their mixture.

  Advantageously, the plant carrier according to the present invention is intended for animal feed and comprises cellulose. Advantageously, the plant carrier according to the present invention is chosen from cereal grains, their authorized products and by-products in animal feed, advantageously from corn, its products and by-products authorized in animal feed. Advantageously, the plant carrier is in solid form, advantageously in powder form, even more advantageously in micronized form. It can also be an oil. Advantageously, the vegetable support represents between 0.1 and 50% by weight of the total weight of the composition, advantageously between 20 and 50% by weight of the total weight of the composition.

  For the purpose of the present invention, food support is understood to mean any raw material authorized for animal feed (according to Decree No. 86-1037 of 15 September 1986). Advantageously, it is selected from the group consisting of the plant carrier as defined above; the mineral support as defined above; authorized dairy products in animal feed; authorized terrestrial animal products for animal feed; fish, other marine animals, their products and by-products authorized for animal feed; and their mixtures. In particular, the authorized dairy products in animal feed are chosen from: skimmed milk powder; buttermilk powder; whey powder; partially de-lactosed powdered whey; whey protein powder; casein (milk) powder; lactose powder and their mixture. In particular, the terrestrial animal products authorized for animal feed are chosen in par- ticular: the meat meal; bone meal; bone meal; greaves (the waste product of tallow, lard or other animal fats extracted or physically separated); poultry meal; flour from hydrolysed feathers; blood meal; animal fats and their mixture.

  In particular fish, other marine animals, their products and by-products authorized in animal feed are chosen among: fish meal; concentrated fish soluble (product obtained in the manufacture of fishmeal and which has been separated and stabilized by acidification or drying); fish oil; the refined hydrogenated fish oil and their mixture. Advantageously, the food carrier according to the present invention is a vegetable carrier according to the present invention, advantageously chosen from cereal grains, their products and by-products authorized in animal feed, advantageously from maize, its authorized products and by-products. in animal feed. Advantageously, the food carrier is in solid form, advantageously in powder form, even more advantageously in micronized form. In a particular embodiment of the present invention, the food carrier according to the present invention is identical to the vegetable carrier according to the present invention, that is to say that the composition according to the present invention comprises a food carrier and the microgranules adsorbed on a carrier. mineral carrier and a vegetable carrier, the vegetable carrier and the food carrier being identical. Advantageously, the food carrier represents between 0.1 and 99.9% by weight of the total weight of the composition, advantageously between 1 and 50% by weight of the total weight of the composition, advantageously between 1 and 10% by weight of the composition. total weight of the composition.

  In a particular embodiment, the active plant extract is present in an amount by weight of between 0.0000 and 99.5% by weight relative to the total weight of the composition, advantageously between 0.2 and 18%. . by weight relative to the total weight of the composition, advantageously between 1 and 10% by weight relative to the total weight of the composition. Advantageously, the composition according to the present invention comprises 9.5% by weight of protein, 16.6% by weight of lipids and 4.1% by weight of crude cellulose. In addition, the SOD content is 2,600,000 UUkg (measured by the OBERLEY ET SPITZ - 198.5 method).

  Advantageously, the composition according to the present invention is in solid form, advantageously in powder form, advantageously having a particle size of between 10 and 250 m.

  Advantageously, the composition according to the present invention is intended for oral administration and advantageously it contains an excipient suitable for oral use in animal feed.

  The present invention furthermore relates to a process for producing a composition according to the present invention comprising the following successive steps: a) melting of the fat-soluble agent, b) deposition of the liquid obtained in step a) on the extract active plant containing the superoxide dismutase, c) adsorption and / or mixing of the microgranules obtained in step b) on and / or with a mineral support, d) adsorption and / or mixing of the adsorbed microgranules and / or the mixture obtained at step c) on and / or with a vegetable support, e) mixing the adsorbed microgranules and / or the mixture obtained in step d) with the food carrier intended for animal feed.

  Advantageously, the active plant extract is in the form of a powder and step b) is carried out in a fluidized air bed, even more advantageously the fluidized bed is at low temperature and low moisture content. In a particular embodiment, the temperature of the fluidized bed of step b) is lower than the melting point of the fat-soluble agent and advantageously is about 40 C. Advantageously, step c) is carried out under a controlled atmosphere. advantageously to obtain a low water content of the inorganic support, advantageously less than 10% by weight relative to the total weight of the inorganic support, and / or step d) is carried out under a controlled atmosphere, advantageously to obtain a low water of the vegetable support, advantageously a water content of less than 12% by weight relative to the total weight of the vegetable support. Advantageously, the microgranules obtained in step (c) are in powder form. Advantageously, the microgranules obtained in step (d) are in the form of powder.

  The present invention further relates to a raw material for animal feed comprising a composition according to the present invention, and advantageously a raw material used in animal feed and / or a raw material for veterinary use suitable for oral use for animal species.

  Advantageously, the raw material used in animal feed is a food carrier according to the present invention.

  The present invention further relates to a composition for veterinary use comprising a composition according to the present invention or a raw material according to the present invention and an excipient suitable for oral use and acceptable from a veterinary point of view for an animal feed. Advantageously, this composition also comprises another antioxidant such as vitamin E or vitamin C.

  Suitable unit dosage forms for the veterinary composition according to the present invention include such forms as tablets, capsules, powders, granules, oral solutions or suspensions, gels, or any other galenic forms used in animals. .

  The present invention also relates to a feed additive premix comprising a composition according to the present invention and a feed additive, advantageously chosen from growth promoters, vitamins (in particular vitamin E and / or C), trace elements, other antioxidants and / or mixtures thereof (mention may be made of acidifiers, plant extracts, aromatic substances, growth factors, alone or in mixtures) or bacterial flora. This premix can therefore be: (1) - partially specific: composition according to the present invention + 1 or 2 other additives with effects comparable to those of the present invention (antioxidant) or having a growth promoter effect such as for example the acidifying agents, plant extracts, aromatic substances, growth factors, alone or in mixtures, incorporated at doses lower than their effective doses, on a support such as, for example, co-products of cereals, calcium carbonate, corn cobs other clays, alone or mixed; (2) non-specific composition according to the present invention incorporated into a complete premix containing at least vitamins and trace elements. In all cases, the premix is incorporated in the final feed distributed to the animals advantageously at rates generally varying from 500 g to 5 kg of premix per ton of feed.

  The present invention further relates to an animal feed comprising a composition according to the present invention or a premix according to the present invention or a raw material according to the present invention. Advantageously, the dose of composition according to the present invention in the animal feed is between 5 and 500 g of composition according to the present invention per ton of feed, advantageously between 20 and 40 g of composition according to the present invention per ton of feed. Advantageously, the food according to the present invention is a dry and liquid compound food, a complete food, a complementary food, a breastfeeding food, a mineral food, a dietary food or a nutritional supplement.

  Thus, the composition according to the present invention may be administered either directly to the animals or in the form of a pharmaceutical composition further comprising an excipient intended for oral administration, or in the form of a premix comprising the composition according to the present invention or the pharmaceutical composition according to the present invention, either by mixing the composition according to the present invention, or the pharmaceutical composition according to the present invention or the premix according to the present invention with the animal feed, advantageously in dietetic food for animals.

  By selecting the individual forms of administration, the specific characteristics of each species as well as the age of the animals must be taken into account. In addition, it must be certain in practice that each animal receives the necessary dose of the composition according to the present invention and that no avoidable loss takes place. The foods containing the composition according to the present invention can be indifferently presented in all the usual forms known in breeding. Food can thus be simple or compound, complete or complementary foods (trace elements, enzymes, acidifiers, aromatic substances and aperitifs, vitamins, ...). In the case where the young animals are always fed by their mother, the composition according to the present invention is preferably injected directly into the throat in the form of a suspension or solute. For calves, the composition according to the present invention can be administered in the form of a milk suspension. Incorporation into drinking water is also possible. For animals that already consume solid food the composition according to the present invention may be mixed with the food. Depending on the animal species, this food can be chosen from grains of cereals, products and by-products; oil seeds and oleaginous fruits, their products and by-products; legume seeds, their products and by-products; tubers and roots, their products and by-products; other 20 seeds and fruits, their products and by-products; fodder, including roughage; other plants, their products and by-products; dairy products ; terrestrial animal products; fish, other marine animals, their products and by-products; the minerals ; and vitamins, alone or as a mixture. A preferred form of administration is in the form of granules which contain, apart from the composition according to the present invention, particularly preferred feed components of the animal in question such as fat, vegetables, animal proteins and the like. vegetable (soybean sprouts, corn sprouts, yeast powder, casein, whey, seaweed powder, fish powder, etc.) with vitamins, salts and trace elements.

  The composition can be administered to fish in the form of capsules having a diameter of 1 to 7 mm which are insoluble in water at room temperature. Another possibility is the administration of fat-containing food granules in which the composition according to the present invention is insoluble or poorly soluble. The incorporation doses of the composition according to the present invention may vary according to the species, the age, the level of ingestion of the animals and, to a certain extent, according to the desired effect. It will be up to the specialist, using systematic tests, to determine for each use the optimal dose.

  The present invention furthermore relates to a composition according to the present invention for its use as a veterinary medicament, advantageously having an antioxidant action in animals, intended to prevent or to treat pathological imbalances in animals caused by free radicals and and / or to prevent or treat oxidative stress in animals and its effects, advantageously to reduce the effects of chronic inflammations in animals (such as arthrosis, tendinitis, laminitis, autoimmune diseases, mastitis causing the presence of cells in the milk, improvement of the implantation of the fertilized ovum during the reproduction of the animals, reduction of the embryonic mortality during the reproduction of the animals ...), and / or to reduce the effects of the stress caused in an animal by a change of temperature, of feeding, density of breeding, of habitat, during competitions (for sports animals for example such as horses, camels, camels or dogs. The composition may be administered before, during, and / or after the competitions), intense and / or prolonged exercise (such as running, working, walking), vaccinations, intensive feeding or during transition periods such as farrowing, spawning, weaning, changing of the pelvis, starting in breeding (such as chickens, nurseries, alvelinage), allotment or transport, and / or slowing down the aging of an animal and to reduce its deleterious effects, and / or to prevent or treat animal reproductive disorders, for example by allowing better maturation of follicles and ova, and / or to prevent or treat specific and non-specific immune disorders in animals, and / or to prevent or treat the aggressions suffered by the animals during the microbial, viral, parasitic attacks and / or to prevent or treat the lack of resistance of their organism to the diseases, and / or to prevent or treat oxidative overloads due to free radicals occurring during the respiration of any living cell, and / or to enhance the action of other antioxidants used in animals.

  Advantageously, the composition according to the present invention is administered to an animal chosen from among livestock, companions or laboratories, advantageously chosen from pigs, cattle, horses, sheep, goats, deer, poultry, poultry, sheep and goats. rabbits, aquaculture animals, fish, crustaceans, (preferably shrimps and crabs), reptiles (turtles, crocodiles), birds, felines, rodents (mice, rats, guinea pigs) and canids, so .beneficial among ruminants and monogastrics.

  The present invention also relates to the use of a composition according to the present invention, a raw material according to the present invention, a premix according to the present invention or a food according to the present invention as an energy supply. , boosting or helping the recovery and fitness of animals. It further relates to the use of a composition according to the present invention, a raw material according to the present invention, a premix according to the present invention or a food according to the present invention to improve the technical quality (advantageously color) and organoleptic meat and fats of the animals who consumed this composition, this premix or this food and increase their shelf life.

  Thus, the consumption by the animals of the composition according to the present invention makes it possible to improve the antioxidant properties of animal feeds, thus enabling a better valorisation of these foods for their health, their vitality, and their aging, as well as an improvement. of meat-derived human nutrition by the very quality of meat and animal fat as well as their suitability for processing. This improvement in the nutritional quality of the animals has a direct impact on the health of the animals and on the health of the consumers.

  Other objects of the invention become apparent to those skilled in the art by reference to the following illustrative drawings: Figure 1 shows the oxidative stress phenomenon and the lines of defense used by the body. FIG. 2 represents the activity of SOD in the plasma in IU / ml in control piglets or whose diet was supplemented with 5 g per ton of food of composition according to example 1 or per 20 g per ton of Composition feeds according to Example 1. Figure 3 shows the amount of HSP 27 protein in the stomach, HSP 90 in the stomach and nNOS in the colon in unit per gram of tissue in control piglets or piglets of which the feed was supplemented with 5 g per ton of composition feed according to example 1 and 20 g per ton of composition feed according to example 1. Figure 4 shows the number of cells per ml of milk in cows with chronic inflammation of the udder before supplementation of their diet with the composition according to Example 1 and during the supplementation of their diet with the composition according to Example 1 over several months over three years. The following examples are given for information only, and are not limiting.

  Example 1 Preparation of a Composition According to the Present Invention

  -Preparation of an active plant extract (described in the patent application FR2716884): 26 5g of pulp of a hybrid cucumis melo derived from the 95LS444 cell line (described in the international application WO 92/02622) are ground in a cold mortar. A 50 mM phosphate buffer volume (pH 7.5, 1 mM EDTA, 5% glycerol) equivalent to 3 times the plant mass is added. After homogenization, the suspension is centrifuged at 5000 g at 4 ° C. for 30 minutes. The supernatant is then recovered and filtered. This crude extract is used to determine superoxide dismutase activity.

  Preparation of microgranules (coated plant active extract: described in patent application FR 2 822 381): The active plant extract prepared above is used. The liposoluble agent used is hydrogenated palm oil. The fusion of this agent is carried out by heating. The liquid agent thus obtained is deposited by spraying with the aid of a bifluid Schlick nozzle onto the powder of the active plant extract suspended in a fluidized air bed at a low temperature (skin temperature below 40 ° C.). C) and low moisture content. The low melting point of this coating agent (58 to 61 ° C) allows instant freezing of the material without burning the active plant extract. The microgranule thus obtained contains 20% by weight of the active plant extract and 80% by weight of the fat-soluble agent.

  Preparation of the Composition According to the Present Invention The microgranules prepared above in the form of a powder are used. The adsorption on the inorganic support is carried out under dry atmosphere (10% moisture content of the support). The mineral support used is dicalcium phosphate. The adsorption on the plant support is carried out under dry atmosphere (moisture content of the support of 12%). The vegetable support used is corn bran. The food support is identical to the vegetable support. The proportions are as follows: 25% microgranules, 30% mineral support, 40% vegetable support, 5% food support Example 2: Resistance of the composition according to the present invention to temperature The resistance of the composition obtained according to the example 1, in particular with regard to its SOD activity, is tested in a water bath at different temperatures for 30 minutes. The activity of the SOD is measured by electrophoretic gel as follows: Sample taking: 3 g of the composition according to Example 1; Dilution in 10 ml of ultra pure water + 0.3% triton; Stirring at 20 ° C. for 30 minutes; Centrifugation at 6-8 C at 12000g for 15 minutes; Recovery of the supernatant; Filtration on cellulose filter at 1.6 m; Volume adjustment at l0m1 with ultra pure water; Electrophoresis gel assay (6% // 10%). Results expressed in units (NBT) of SOD / mg 100% = 2.6 IU of SOD / mg The results are summarized in Table 1 below: Table 1: SOD activity of the composition according to Example 1 after 30 minutes in a bain-marie at different temperatures. Temperature (C) 25 35 45 55 65 75 Activity SOD (%) 100 98.7 96.3 91.6 87.9 40.6 The composition according to the present invention is thus stable up to a temperature of 65 C, advantageously less than 55 C. It is therefore adapted to the specific constraints of certain technologies applied in animal feed industries, in particular because of its resistance to heating during granulation or extrusion. Example 3: Stability of the composition according to the present invention on storage The stability of the composition according to Example 1 is measured by assaying the SOD activity after several months of storage at 14 ° C. The activity of the SOD is measured as indicated in Example 2 The results are summarized in Table 2 below: Table 2: SOD activity of the composition according to Example 1 after storage at 10 14 C as a function of the storage duration The composition according to the present invention is thus stable for at least 22 months at a temperature of 14 ° C., advantageously for at least 9 months at a temperature of about 10%. 14 C.

  Example 4 Bioavailability of the Composition According to the Present Invention: Measurement of the Plasma SOD Increase

  Experimental Protocol 48 piglets were weaned at 21 days of age. They did not have access to solid foods before weaning. In addition, a 2-day fast (with access to water) was imposed immediately post-weaning, so as to exacerbate the alterations of the digestive tract. Piglets of different litters were mixed for 24 hours to exacerbate the stress. Finally, they were raised in individual cages, in rooms whose hygiene was deliberately kept at a mediocre level, so as to increase the risk of health problems. 29 A second-age food based on cereals and vegetable proteins (wheat, barley, soybean meal) and containing no food additive antibiotics was formulated and delivered in granules (Table 3). It was used to prepare three experimental foods, different by the level of incorporation of the composition according to example 1. T: control food containing no composition according to example 1. EV1: food containing 5 g of composition according to Example 1 per ton. EV2: food containing 20 g of composition according to Example 1 per ton.

  Table 3. Composition of Experimental Feed Piglets 2nd "Senior Ingredient (g / kg) EV1 EV2 Wheat 232.07 232.07 232.07 Maize 279.87 279.87 279.87 Barley 172.48 172.48 172.48 Soybean meal 48 267.23 267.23 267.23 Plant oil 4.48 4.48 4.48 Calcium carbonate 6.35 6.35 6.35 Calcium phosphate 16.14 16.14 16.14 Salt 3.98 3.98 3.98 CMV 2nd age 4.98 4.98 4.98 Lysine 4.36 4.36 4.36 Methionine 1.04 1.04 1.04 Threonine 2.16 2.16 2 , 16 Tryptophan 0.87 0.87 0.87 Composition according to Example 1 0.00 1.00 4.00 (diluted to 1 / 200th) Dilution support of 4.00 3.00 0.00 composition according to Example 1 EXAMPLE 1 Total 1000 1000 1000 The composition according to Example 1 is introduced in a form diluted 1 / 200th in a dilution medium: dried co-product powder of the wet millet corn. dilution of the composition according to Example 1, this dilution medium was incorporated into the control foods and EV1 at a rate of 4 and 3 g per kg of feed (T. The results of chemical analyzes of experimental foods (T, EV1, EV2) are presented in Table 4 below:

  Table 4: Analytical composition of experimental feeds EV EV21 EV21 Mean (SD) Mean (SD) Avg. (SD) Dry matter 89.53 (0.13) 89.44 (0.12) 89.55 (0.16) (% DM) Mineral matter 6.00 (002) 5.88 (0.01) 6 , 01 (0.01) (% MS) Nitrogen material 22.6 7 (0.18) 22.75 (0, 24) 22.74 (0.18) total (% MS) Average of 4 determinations (2 for the first repetition and 2 for the second repetition) SD = standard deviation

  The second-age food was degranulated, the composition according to Example 1 and the dilution support were incorporated, then each experimental food was regranulated. A preliminary test showed the good resistance of the product to granulation under experimental conditions. Piglets were fed under the following conditions: distributed quantities in triplets aligned daily on the lowest individual triplet-feeding. Two replicates were conducted on 48 piglets, ie 24 piglets per repetition.

  Piglets were divided into intra-lit quadruplets of the same live weight at weaning (1 or 2 quadruplets per litter depending on the number and homogeneity of the piglets). Each quadruplet is therefore composed of: - a piglet shot at 2 days (thus having fasted 2 days). - three piglets all slaughtered at 7 days or 14 days. The weight balance between groups D + 2, D + 7 and D + 14 has been checked globally. The results were analyzed using GLM (Generali Linear Models) procedures of the SAS software (Statistical Analysis System). The effect of slaughter age was tested using the residual variation between quadruplets as an error. The effect of the treatment and the interaction treatment x age of slaughter were tested by taking the residual intra-quadruplet variation as an error. In the treatment effect, the food treatments T, EV 1 and EV 2 were considered, but also the J + 2 treatment, a piglet of each quadruplet `D + 7 'or` D + 14' having been shot at D + 2.

  Results Granulation test and resistance of the composition according to Example 1 A granulation test (by steam press and compression through a die) after incorporation of the composition according to Example 1 was carried out at using a non-experimental commercial age feed. The test covered 5 kg of food and the incorporation rates of the composition according to Example 1 were 50 and 250 g / kg of food. These incorporation rates are very high compared to the experimental doses. They have been set to better detect the biological activity of SOD. Two kilograms of each food were collected in the manufacturing environment and SOD activity was analyzed (measured by OBERLEY AND SPITZ - 1985). The press outlet temperature was close to 41 C. The granulation conditions of this test had no effect on the SOD activity of the two prepared foods. Thus, the SOD content of the composition according to the present invention is identical to that of the control, ie 2600000 IU SOD / g.

  Conduct of the experiment Overall, the in vivo test was conducted without any particular problems. No health problems were recorded, which resulted in the total absence of drug treatments.

  Growth and consumption Piglets weighed an average of 6 kg at batching (D-1 before weaning) and a little less after post-weaning fast (D + 2). They weighed an average of 10 6.25 and 7 kg at slaughter on days D + 7 and D + 14 post-weaning, respectively.

  Plasma SOD Concentration Results The results are summarized in Table 5 below and in FIG.

  Table 5: Influence of treatment and day of slaughter on plasma superoxide dismutase (SOD) and intestinal mucosal contents of weaned pigs (Lsmeans and SE) Slaughter (Ab) D + 2 D + 7 D + 2 D + 14 Probability of effects Variable / J2 EV1 EV2 T4 Ev1 EV2 EV2 SE Tr2 Ab Tr * Ab SOD Plasma 381 366 370 341 385 269 335 379 26 0.1116 0.40 0.0825 (IU / ml) Tr (J2, Tem, EV1, EV2) (treatment effect tested against intra-quadruplets error), Ab slaughter (J7 vs J14), Tr * Ab

  These results show that the concentration of plasma SOD tends to be higher, at the end of the test, with the highest composition dose according to Example 1, relative to the control. In reality, this effect was proportional to the composition dose according to Example 1. These results show the bioavailability of the SOD of the composition according to the present invention since the composition according to Example 1 makes it possible to increase by 25%. at 40% activity SOI) of plasma. Example 3: Protective effect against stress

  At the biochemical level, stress (environmental change, transport, bacterial or viral infections, etc.) is accompanied by variations of a certain number of markers that can be demonstrated by analysis. An in vivo study was conducted on piglets under stress: the weaning period. The experimental protocol is identical to that of Example 2 above. Markers of oxidative stress, heat shock proteins (HSP) and NOS (NO synthase), are measured in gastrointestinal tissues (measurement method Gerlach et al (1995) 6: 1141-1145). These molecules are produced by the cells in response to stress conditions. The results are collated in FIG. 3. Thus, the highest composition dose according to example 1 seems to have more effects than the lowest dose. At 20 g per ton of feed the composition according to Example 1 almost invariably reduces the tissue concentrations of cytoprotective proteins, either moderately (but significantly, for HSP 27 to J14 and HSP 70 to J + 7 + J + 14 in the jejunum, nNOS at D + 14 in the stomach) or very markedly (HSP 27 in the stomach and J + 7 in the jejunum, HSP 70 in the colon at D + 7, HSP 90 in the stomach, nNOS in jejunum and colon). In addition, it seems that several effects are proportional to the dose of composition according to Example 1, for example HSP 27 in the stomach (J + 14) and in the jejunum (J + 7), HSP 70 in the colon (J + 7) and nNOS in the colon (D + 14). Overall, these observations could mean less stress with the supplemented composition diet according to Example 1, particularly at the concentration of 20 g per ton of feed. Thus, the composition of the present invention significantly reduces (about 50 %) Tissue concentrations of NOS and HSP in piglets at weaning. It has a protective effect against some deleterious effects of stress. EXAMPLE 4 Effects of the Composition According to Example 1 on Mastitis by Counting the Cells of the Mixing Milk of Dairy Cows

  10 One of the criteria for assessing the quality of the milk of a cattle farm is the measurement of the level of cells contained in it which reflects the state of health of the cow's udder and the presence or absence of mastitis. When the level of cells contained in the milk is less than 250 000 cells / ml, the udder is in good health. During inflammation of the udder (mastitis), the milk cell level increases. From 400 000 cells / ml, the milk is unfit for human consumption. The effect of the composition according to Example 1 on inflammation of the udder was tested for 18 months in a breeding of 35 dairy cows suffering from chronic inflammation. The feed of this herd of 35 cows was supplemented with 50 mg of composition according to Example 1 per day and per cow for 18 months, ie a dose of 160 mg / kg. The dairy cow herd was fed before and during the experiment with a daily winter ration comprising on average 44 kg of corn silage, 5 kg of soybean cake 48, 1 kg of English fattening hay and 300 g of mineral. trade type 4/21/5. From May to September they benefited from grazing English and Italian fats with about 5 kg of corn silage. The URCILL, the official control body in France, carries out the cellular counting of the mixture milks each month. The results are shown in FIG. 4.5. The URCILL count shows that there is a decrease in inflammatory cells of the milk by about 60% from the start of the distribution of the composition according to Example 1 in FIG. feeding of dairy cows.

  The consumption of the composition according to the present invention by dairy cows thus significantly reduces the inflammatory cells of the milk.

Claims (23)

  A composition comprising a feed carrier for animal feed and microgranules adsorbed on and / or mixed with a mineral carrier and adsorbed onto and / or mixed with a plant carrier, said microgranules being an active plant extract containing superoxide dismutase, said extract being microencapsulated in a liposoluble agent based on fat.   2. Composition according to claim 1, characterized in that the plant extract is a protein extract of Cucumis melo, preferably a water-soluble extract.   3. Composition according to claim 2, characterized in that the Cucumis melo 15 is a descendant of the 95LS444 cell line or one of the hybrid lines derived from 95LS444.   4. A composition according to any one of the preceding claims characterized in that the fat-soluble agent has a melting point of from about 40 to about 80 ° C.   5. Composition according to claim 4, characterized in that the fat-soluble agent is hydrogenated oil based on vegetable oil, in particular chosen from the group consisting of hydrogenated coconut oil, hydrogenated palm oil, Hydrogenated soybean oil or hydrogenated rapeseed oil, preferably hydrogenated palm oil.   6. Composition according to any one of the preceding claims, characterized in that the mineral carrier is chosen from the calcium sources 30 authorized in animal feed, advantageously in solid form.   7. Composition according to any one of the preceding claims, characterized in that the plant carrier is intended for animal feed and comprises cellulose, advantageously it is selected from corn, its products and by-products authorized in animal feed .   8. Composition according to any one of the preceding claims, characterized in that the food carrier for animal feed is identical to the vegetable carrier. 10   9. Composition according to any one of the preceding claims, characterized in that the active plant extract is present in a concentration by weight of between 0.0001 and 99.5% relative to the total weight of the composition. 15   10. Composition according to any one of the preceding claims, characterized in that it is intended for oral administration and advantageously in that it contains an excipient suitable for oral use in animal feed. 20   11. A method of manufacturing a composition according to any one of claims 1 to 10, characterized in that it comprises the following successive stages: a) melting of the fat-soluble agent, b) deposition of the liquid obtained in the step a) on the active plant extract containing the superoxycle dismutase, c) adsorption and / or mixing of the microgranules obtained in step b) on and / or with a mineral support, d) adsorption and / or mixing of the adsorbed microgranules and / or the mixture obtained in step c) on and / or with a vegetable carrier, e) mixing the adsorbed microgranules and / or the mixture obtained in step d) with the feed carrier intended for animal feed .5   12. The method of claim 11, characterized in that step c) is performed under controlled atmosphere and step d) is carried out under controlled atmosphere.   13. Raw material for animal feed comprising a composition according to any one of claims 1 to 12, and advantageously a raw material used in animal feed and / or a raw material for veterinary use suitable for oral use for animal species.   14. Composition for veterinary use comprising a composition according to any one of claims 1 to 10 or a raw material according to claim 13 and an excipient suitable for veterinally acceptable oral use for animal feed.   15. Premix for an animal feed additive comprising a composition according to any one of claims 1 to 10 and a feed additive for animals, advantageously chosen from growth promoters, bacterial flora, vitamins, other antioxidants, trace elements and / or their mixtures.   An animal feed comprising a composition according to any one of claims 1 to 10 or a premix according to claim 15 or a raw material according to claim 13.   17. Food according to claim 16 characterized in that it is a dry and liquid compound food, a complete food, a complementary food, a breastfeeding food, a mineral food , a dietary food or a nutritional supplement.   18. A composition according to any one of claims 1 to 10 or 14 for use as a veterinary medicament.   19. Composition according to claim 18 for its use as a veterinary medicinal product having an antioxidant action in animals, intended to prevent or treat the pathological imbalances in animals caused by free radicals and / or to prevent or treat stress in the animal and its effects, advantageously to reduce the effects of chronic inflammations, and / or to reduce the effects of stress caused in an animal by a change in temperature, diet, stocking density, d habitat, during competitions, intense and / or prolonged exercise, vaccination, intensive feeding, or during transitional periods such as birthing, spawning, weaning, pelvis switching, start-up in breeding, allotment or transport, and / or to slow the aging of the animal and to reduce its deleterious effects and / or to prevent or treat the animal reproductive disorders and / or to prevent or treat specific and non-specific immune disorders of the animals, and / or to prevent or treat the aggressions suffered by the animals during the microbial, viral, parasitic, and / or to prevent or treat the lack of resistance of their animals. organism to diseases, and / or to prevent or treat oxidative overloads and / or to enhance the action of other antioxidants used in animals ...   20. Composition according to claim 19, characterized in that the animal is chosen from livestock, companion animals or laboratories, advantageously chosen from pigs, cattle, horses, sheep, goats, deer, poultry, rabbits, aquaculture animals, fish, crustaceans, reptiles birds, felines, rodents and canids, advantageously among ruminants and monogastrics.   21. Use of a composition according to any one of claims 1 to 10, a raw material according to claim 13, a premix according to claim 15 or a foodstuff according to any one of claims 16. or 40 17 as an energy supply, a boosting factor or aiding the recovery and fitness of animals.   22. Use of a composition according to any one of claims 1 to 10, a raw material according to claim 13, a premix according to claim 15 or a food according to any one of claims 16 or 17 to improve the technical and organoleptic quality of the meat and fats of the animals having consumed this composition, this premix or this food, and to increase their shelf life.   23. Use according to any one of claims 21 or 22, characterized in that the animal is selected from livestock, companion or laboratory, preferably selected from pigs, cattle, horses, sheep, goats, deer, poultry, rabbits, aquaculture animals, fish, crustaceans, reptiles birds, felines, rodents and canids, advantageously among ruminants and monogastrics.