FR2533583A1 - Nouvel adn recombinant utile dans la preparation d'a-amylase - Google Patents
Nouvel adn recombinant utile dans la preparation d'a-amylase Download PDFInfo
- Publication number
- FR2533583A1 FR2533583A1 FR8216099A FR8216099A FR2533583A1 FR 2533583 A1 FR2533583 A1 FR 2533583A1 FR 8216099 A FR8216099 A FR 8216099A FR 8216099 A FR8216099 A FR 8216099A FR 2533583 A1 FR2533583 A1 FR 2533583A1
- Authority
- FR
- France
- Prior art keywords
- amylase
- dna
- strain
- phage
- plasmid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000004382 Amylase Substances 0.000 title claims abstract description 28
- 108020004511 Recombinant DNA Proteins 0.000 title claims abstract description 11
- 238000002360 preparation method Methods 0.000 title description 9
- 108020004414 DNA Proteins 0.000 claims abstract description 35
- 239000013612 plasmid Substances 0.000 claims abstract description 24
- 239000012634 fragment Substances 0.000 claims abstract description 20
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 18
- 230000001580 bacterial effect Effects 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 17
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 9
- 230000008569 process Effects 0.000 claims abstract description 8
- 108090000637 alpha-Amylases Proteins 0.000 claims description 32
- 102000004139 alpha-Amylases Human genes 0.000 claims description 32
- 229940024171 alpha-amylase Drugs 0.000 claims description 28
- 102000013142 Amylases Human genes 0.000 claims description 21
- 108010065511 Amylases Proteins 0.000 claims description 21
- 235000019418 amylase Nutrition 0.000 claims description 21
- 241000588724 Escherichia coli Species 0.000 claims description 13
- 241000702208 Shigella phage SfX Species 0.000 claims description 8
- 230000003204 osmotic effect Effects 0.000 claims description 4
- 230000035939 shock Effects 0.000 claims description 4
- 108020000946 Bacterial DNA Proteins 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 229920002472 Starch Polymers 0.000 description 19
- 235000019698 starch Nutrition 0.000 description 19
- 239000008107 starch Substances 0.000 description 19
- 102000004190 Enzymes Human genes 0.000 description 16
- 108090000790 Enzymes Proteins 0.000 description 16
- 229940088598 enzyme Drugs 0.000 description 16
- 230000009471 action Effects 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 8
- 230000003625 amylolytic effect Effects 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 239000008103 glucose Substances 0.000 description 7
- 208000015181 infectious disease Diseases 0.000 description 7
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 6
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 6
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 230000009089 cytolysis Effects 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 229940025131 amylases Drugs 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 239000004098 Tetracycline Substances 0.000 description 3
- 229960000723 ampicillin Drugs 0.000 description 3
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 108010075550 termamyl Proteins 0.000 description 3
- 229960002180 tetracycline Drugs 0.000 description 3
- 229930101283 tetracycline Natural products 0.000 description 3
- 235000019364 tetracycline Nutrition 0.000 description 3
- 150000003522 tetracyclines Chemical class 0.000 description 3
- 241001515965 unidentified phage Species 0.000 description 3
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
- 229920000945 Amylopectin Polymers 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 102000012410 DNA Ligases Human genes 0.000 description 2
- 108010061982 DNA Ligases Proteins 0.000 description 2
- 241000193385 Geobacillus stearothermophilus Species 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 229910001424 calcium ion Inorganic materials 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 239000013599 cloning vector Substances 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 230000003301 hydrolyzing effect Effects 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 229910052740 iodine Inorganic materials 0.000 description 2
- 239000011630 iodine Substances 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000003362 replicative effect Effects 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000004753 textile Substances 0.000 description 2
- WDMUXYQIMRDWRC-UHFFFAOYSA-N 2-hydroxy-3,4-dinitrobenzoic acid Chemical compound OC(=O)C1=CC=C([N+]([O-])=O)C([N+]([O-])=O)=C1O WDMUXYQIMRDWRC-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 229920000856 Amylose Polymers 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 229920002245 Dextrose equivalent Polymers 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241001131785 Escherichia coli HB101 Species 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 241000702192 Escherichia virus P2 Species 0.000 description 1
- 241000701533 Escherichia virus T4 Species 0.000 description 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 208000035415 Reinfection Diseases 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000001166 ammonium sulphate Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229940097362 cyclodextrins Drugs 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000001738 isopycnic centrifugation Methods 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 230000001320 lysogenic effect Effects 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 210000001322 periplasm Anatomy 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8216099A FR2533583A1 (fr) | 1982-09-24 | 1982-09-24 | Nouvel adn recombinant utile dans la preparation d'a-amylase |
| FR8220904A FR2537602B2 (fr) | 1982-09-24 | 1982-12-13 | Nouvel adn recombinant perfectionne utile dans la preparation d'a-amylase par bacillus subtilis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8216099A FR2533583A1 (fr) | 1982-09-24 | 1982-09-24 | Nouvel adn recombinant utile dans la preparation d'a-amylase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2533583A1 true FR2533583A1 (fr) | 1984-03-30 |
| FR2533583B1 FR2533583B1 (enrdf_load_stackoverflow) | 1985-02-01 |
Family
ID=9277706
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR8216099A Granted FR2533583A1 (fr) | 1982-09-24 | 1982-09-24 | Nouvel adn recombinant utile dans la preparation d'a-amylase |
Country Status (1)
| Country | Link |
|---|---|
| FR (1) | FR2533583A1 (enrdf_load_stackoverflow) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0149915A3 (en) * | 1983-12-19 | 1986-12-30 | G.D. Searle & Co. | Starch utilization by gram negative microorganisms |
| FR2598431A1 (fr) * | 1986-05-09 | 1987-11-13 | Transgene Sa | Souches de saccharomyces produisant de l'alpha-amylase |
| FR2613727A2 (fr) * | 1986-06-10 | 1988-10-14 | Transgene Sa | Integration dans la souche polyploide saccharomyces uvarum 2285 tg2 amgc du bloc d'expression de l'alpha-amylase de b. licheniformis |
| EP2192371A2 (de) | 2008-11-12 | 2010-06-02 | Viktor Schäfer | Flüssigkeits-Wärmespeicher mit horizontaler Temperaturschichtung |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2035875A1 (enrdf_load_stackoverflow) * | 1969-03-25 | 1970-12-24 | Rolland Sa A | |
| EP0032238A2 (en) * | 1980-01-07 | 1981-07-22 | The University Of Rochester | Recombinant bacteriophage for heterologous cloning of bacillus microorganisms and method for its production |
| EP0034470A2 (en) * | 1980-02-15 | 1981-08-26 | Cpc International Inc. | Genetically engineered microorganisms for massive production of amylolytic enzymes and process for preparing same |
| EP0036259A2 (en) * | 1980-03-10 | 1981-09-23 | Cetus Corporation | Method,vectors and organisms for transported cloned heterologous gene products in bacillus subtilis and E.coli |
| EP0057976A2 (en) * | 1981-01-15 | 1982-08-18 | Cpc International Inc. | A process for cloning the gene coding for a thermostable alpha-amylase into escherichia coli and bacillus subtilis |
-
1982
- 1982-09-24 FR FR8216099A patent/FR2533583A1/fr active Granted
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2035875A1 (enrdf_load_stackoverflow) * | 1969-03-25 | 1970-12-24 | Rolland Sa A | |
| EP0032238A2 (en) * | 1980-01-07 | 1981-07-22 | The University Of Rochester | Recombinant bacteriophage for heterologous cloning of bacillus microorganisms and method for its production |
| EP0034470A2 (en) * | 1980-02-15 | 1981-08-26 | Cpc International Inc. | Genetically engineered microorganisms for massive production of amylolytic enzymes and process for preparing same |
| EP0036259A2 (en) * | 1980-03-10 | 1981-09-23 | Cetus Corporation | Method,vectors and organisms for transported cloned heterologous gene products in bacillus subtilis and E.coli |
| EP0057976A2 (en) * | 1981-01-15 | 1982-08-18 | Cpc International Inc. | A process for cloning the gene coding for a thermostable alpha-amylase into escherichia coli and bacillus subtilis |
Non-Patent Citations (2)
| Title |
|---|
| CHEMICAL ABSTRACTS, vol. 95, no. 17, 26 octobre 1981, page 333, no. 146979j, Columbus Ohio (USA); * |
| JOURNAL OF BACTERIOLOGY, vol. 145, no. 1, janvier 1981, pages 442-428; * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0149915A3 (en) * | 1983-12-19 | 1986-12-30 | G.D. Searle & Co. | Starch utilization by gram negative microorganisms |
| FR2598431A1 (fr) * | 1986-05-09 | 1987-11-13 | Transgene Sa | Souches de saccharomyces produisant de l'alpha-amylase |
| EP0252774A1 (fr) * | 1986-05-09 | 1988-01-13 | Transgene S.A. | Souches de saccharomyces produisant de l'alpha-amylase |
| FR2613727A2 (fr) * | 1986-06-10 | 1988-10-14 | Transgene Sa | Integration dans la souche polyploide saccharomyces uvarum 2285 tg2 amgc du bloc d'expression de l'alpha-amylase de b. licheniformis |
| EP2192371A2 (de) | 2008-11-12 | 2010-06-02 | Viktor Schäfer | Flüssigkeits-Wärmespeicher mit horizontaler Temperaturschichtung |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2533583B1 (enrdf_load_stackoverflow) | 1985-02-01 |
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