FR2454464A1 - Continuous Clostridium species spore prodn. - using multi-fermenter system - Google Patents

Continuous Clostridium species spore prodn. - using multi-fermenter system

Info

Publication number
FR2454464A1
FR2454464A1 FR7922694A FR7922694A FR2454464A1 FR 2454464 A1 FR2454464 A1 FR 2454464A1 FR 7922694 A FR7922694 A FR 7922694A FR 7922694 A FR7922694 A FR 7922694A FR 2454464 A1 FR2454464 A1 FR 2454464A1
Authority
FR
France
Prior art keywords
fermenter
prodn
dilution rate
increased
fermenters
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7922694A
Other languages
French (fr)
Other versions
FR2454464B1 (en
Inventor
Koki Horikoshi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Miyarisan Pharmaceutical Co Ltd
Original Assignee
Miyarisan Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miyarisan Pharmaceutical Co Ltd filed Critical Miyarisan Pharmaceutical Co Ltd
Publication of FR2454464A1 publication Critical patent/FR2454464A1/en
Application granted granted Critical
Publication of FR2454464B1 publication Critical patent/FR2454464B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N3/00—Spore forming or isolating processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Prodn. Clostridium butyricum Miyairi (popular name; Miyairi-Kin) spores by culturing continuously using multi fermenter system comprises (a) setting the dilution rate of the first fermenter to a prescribed range. (b) setting the dilution rate of the other fermenters according to the first fermenter and (c) using several fermenters alternately as a first fermenter. The first to third fermenters are used for forming nutritive cells, endospores and matured spores respectively and in them the dilution rate is set at 0.75/hr. (0.3-0.6 hr) 0.06-0.07/hr and 0.04-0.05/hr respectively. Mixt. of sugar (e.g. corn starch), amino acid (e.g. amino acid mixt. obtd. by hydrolysing defatted soy bean) and inorganic salt (e.g. CaCO3), can be favorably used as the culture medium. Prodn. depends upon the nutritive conditions in the first fermenter and the form of the cells in the first fermenter is changed after 100 hours so that when culturing is continued, the prodn. of the spore in the third fermenter falls. By switching the first fermenter at proper intervals the prodn. can be continuous with high yield. The yield per vol. of culture liq. is increased 3 times that of prior art and the dilution rate is increased 5 times. Prodn. per hour is therefore increased 15 times.
FR7922694A 1979-04-21 1979-09-11 Continuous Clostridium species spore prodn. - using multi-fermenter system Granted FR2454464A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4922479A JPS55141191A (en) 1979-04-21 1979-04-21 Production of spore of clostridium butyricum miyairi

Publications (2)

Publication Number Publication Date
FR2454464A1 true FR2454464A1 (en) 1980-11-14
FR2454464B1 FR2454464B1 (en) 1984-02-10

Family

ID=12824950

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7922694A Granted FR2454464A1 (en) 1979-04-21 1979-09-11 Continuous Clostridium species spore prodn. - using multi-fermenter system

Country Status (3)

Country Link
JP (1) JPS55141191A (en)
FR (1) FR2454464A1 (en)
PH (1) PH15484A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110151795A (en) * 2019-05-14 2019-08-23 广东大泽农生物科技股份有限公司 A kind of clostridium butyricum active bacteria preparation and its production technology

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110408563B (en) * 2019-07-17 2021-05-25 天津市圣世莱科技有限公司 High-density fermentation of clostridium butyricum and preparation method of microecological microbial agent thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR61040E (en) * 1950-11-09 1955-03-23 Centre Nat Rech Scient High-yield method and device for the continuous cultivation of microorganisms

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR61040E (en) * 1950-11-09 1955-03-23 Centre Nat Rech Scient High-yield method and device for the continuous cultivation of microorganisms

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DW1968 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110151795A (en) * 2019-05-14 2019-08-23 广东大泽农生物科技股份有限公司 A kind of clostridium butyricum active bacteria preparation and its production technology
CN110151795B (en) * 2019-05-14 2020-03-17 广东大泽农生物科技股份有限公司 Clostridium butyricum viable bacteria preparation and production process thereof

Also Published As

Publication number Publication date
JPS55141191A (en) 1980-11-04
PH15484A (en) 1983-01-27
FR2454464B1 (en) 1984-02-10
JPS5653353B2 (en) 1981-12-18

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Legal Events

Date Code Title Description
ST Notification of lapse