GB1290694A - - Google Patents

Abstract

1290694 Dextranase TATE & LYLE Ltd 11 Nov 1970 [12 Nov 1969] 55403/69 Heading C3H [Also in Division C6] Dextranase to be used to degrade dextran present in a sucrose product during the prodution of raw sugar from sugar cane or during the refining of raw cane sugar is obtained by the growth of a dextranase-producing microorganism, e.g. Penicillium funiculosum or Penicillium lilacinum, in the presence of dextran as the principal or sole carbon source. This dextran may be obtained by growing Leuconostoc mesenteroides in the presence of sucrose, cane juice, molasses, &c. Several strains of Leuconostoc mesenteroides may be used to produce a mixture of dextrans to simulate the mixture to be found in the sucrose product to be treated. The Leuconostoc mesenteroides is grown for a period of 16 to 20 hours at a temperature of 25 to 30‹ C. under micro aerophilic conditions with an initial pH of 6À8 to 7À2. The diluted crude culture filtrate, after removal of the Leuconostoc mesenteroides and sugar, is used as a growth medium for Penicillium funiculosum &c. at a temperature of 25‹ to 35‹ C. and a pH of 5À5 to 7.0 for 72 to 120 hours, and the resulting dextranase is recovered as a solid or liquid concentrate. Example 6 describes the treatment of dextrancontaining raw sugar juice with a mixture of dextranase and an amylase, e.g. fungal α- amylase, bacterial α-amylase or gluco-amylase.