FR2431696A1 - Microfluorometre a circulation - Google Patents

Microfluorometre a circulation

Info

Publication number
FR2431696A1
FR2431696A1 FR7918793A FR7918793A FR2431696A1 FR 2431696 A1 FR2431696 A1 FR 2431696A1 FR 7918793 A FR7918793 A FR 7918793A FR 7918793 A FR7918793 A FR 7918793A FR 2431696 A1 FR2431696 A1 FR 2431696A1
Authority
FR
France
Prior art keywords
microfluorometer
wavelength
particle
variation
circulation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7918793A
Other languages
English (en)
Other versions
FR2431696B3 (fr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of FR2431696A1 publication Critical patent/FR2431696A1/fr
Application granted granted Critical
Publication of FR2431696B3 publication Critical patent/FR2431696B3/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Signal Processing (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)

Abstract

L'invention concerne un microfluoromètre à circulation. La variation de l'intensité de la fluorescence d'une particule en fonction de la longueur d'onde est transformée en variation électrique au cours du temps, et le temps de variation du signal est proportionnel à la longueur d'onde. A cet effet, un dispositif 10 transmet des particules sur une droite de focalisation d'un faisceau d'excitation, et l'image de la particule est formée sur un filtre en coin 28 dont la lumière est focalisée sur un tube photomultiplicateur 32. Application à l'analyse par fluorescence d'humeurs et de liquides biologiques.
FR7918793A 1978-07-21 1979-07-20 Microfluorometre a circulation Granted FR2431696A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US05/926,759 US4172227A (en) 1978-07-21 1978-07-21 Flow microfluorometer

Publications (2)

Publication Number Publication Date
FR2431696A1 true FR2431696A1 (fr) 1980-02-15
FR2431696B3 FR2431696B3 (fr) 1981-05-29

Family

ID=25453672

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7918793A Granted FR2431696A1 (fr) 1978-07-21 1979-07-20 Microfluorometre a circulation

Country Status (4)

Country Link
US (1) US4172227A (fr)
DE (1) DE2929170C2 (fr)
FR (1) FR2431696A1 (fr)
GB (1) GB2032097B (fr)

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FR2500164A1 (fr) * 1981-02-13 1982-08-20 Fella Christian Procede de mise en evidence des antigenes de groupe sanguin a, b, h sur des cellules humaines en milieu liquide
US4629687A (en) * 1982-07-29 1986-12-16 Board Of Trustees Of Michigan State University Positive selection sorting of cells
US4624915A (en) * 1982-07-29 1986-11-25 Board Of Trustees Of Michigan State University Positive selection sorting of cells
US4492752A (en) * 1982-09-03 1985-01-08 Ortho Diagnostics Systems Inc. Method for discriminating between unstained and absorbing dye stained cells
US4837446A (en) * 1988-03-31 1989-06-06 International Paper Company Apparatus and process for testing uniformity of pulp
JP3181609B2 (ja) * 1990-12-28 2001-07-03 株式会社島津製作所 キャピラリークロマトグラフィー用光検出器
US5203339A (en) * 1991-06-28 1993-04-20 The Government Of The United States Of America As Represented By The Secretary Of The Department Health And Human Services Method and apparatus for imaging a physical parameter in turbid media using diffuse waves
US5484571A (en) * 1991-10-08 1996-01-16 Beckman Instruments, Inc. Enhanced fluorescence detection of samples in capillary column
ES2123063T3 (es) 1992-09-14 1999-01-01 Stanford Res Inst Int Marcadores convertidores al alza para ensayos biologicos y otros mediante tecnicas de excitacion laser.
US5469255A (en) * 1993-01-28 1995-11-21 Kamada; Kenji Method and apparatus for spectrometric measurement of particulate surfaces
US5528045A (en) * 1995-04-06 1996-06-18 Becton Dickinson And Company Particle analyzer with spatially split wavelength filter
WO1998057152A1 (fr) 1997-06-09 1998-12-17 Guava Technologies, Inc. Procede et dispositif pour deceler des microparticules dans des echantillons de fluides
US6710871B1 (en) 1997-06-09 2004-03-23 Guava Technologies, Inc. Method and apparatus for detecting microparticles in fluid samples
US6372895B1 (en) 2000-07-07 2002-04-16 3M Innovative Properties Company Fluorogenic compounds
US6544396B1 (en) * 2000-07-20 2003-04-08 Symyx Technologies, Inc. Multiplexed capillary electrophoresis system
US6531041B1 (en) 2000-07-20 2003-03-11 Symyx Technologies, Inc. Multiplexed capillary electrophoresis system with rotatable photodetector
US6572750B1 (en) 2000-07-21 2003-06-03 Symyx Technologies, Inc. Hydrodynamic injector
US6462816B1 (en) 2000-07-21 2002-10-08 Symyx Technologies, Inc. Parallel capillary electrophoresis system having signal averaging and noise cancellation
US20030124592A1 (en) * 2001-10-24 2003-07-03 Bioprofile, Llc Methods for detecting genetic haplotypes by interaction with probes
US20090088982A1 (en) * 2003-07-31 2009-04-02 Fukushima Noelle H Co-detection of single polypeptide and polynucleotide molecules
US20080021674A1 (en) * 2003-09-30 2008-01-24 Robert Puskas Methods for Enhancing the Analysis of Particle Detection
EP1779088B1 (fr) * 2004-08-06 2016-03-09 Compucyte Corporation Absorption de lumiere monochromatique a couleurs multiples et quantification d'absorption de lumiere dans un echantillon colore
US7572640B2 (en) * 2004-09-28 2009-08-11 Singulex, Inc. Method for highly sensitive detection of single protein molecules labeled with fluorescent moieties
US8685711B2 (en) * 2004-09-28 2014-04-01 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
US20060078998A1 (en) * 2004-09-28 2006-04-13 Singulex, Inc. System and methods for sample analysis
US9040305B2 (en) * 2004-09-28 2015-05-26 Singulex, Inc. Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry
WO2006086382A2 (fr) 2005-02-08 2006-08-17 Northrop Grumman Corporation Systeme et procedes utilises dans la detection de particules aerosols nocives
US7838250B1 (en) 2006-04-04 2010-11-23 Singulex, Inc. Highly sensitive system and methods for analysis of troponin
JP5308328B2 (ja) 2006-04-04 2013-10-09 シングレックス，インコーポレイテッド トロポニンの分析のための高感度のシステムおよび方法
JP5315574B2 (ja) * 2007-03-22 2013-10-16 富士フイルム株式会社 撮像装置
US7914734B2 (en) 2007-12-19 2011-03-29 Singulex, Inc. Scanning analyzer for single molecule detection and methods of use
CN102016552A (zh) * 2008-03-05 2011-04-13 神谷来克斯公司 用于分子的高灵敏性检测的方法和组合物
DE102008029700A1 (de) * 2008-06-24 2010-01-14 Palas Gmbh Partikel- Und Lasermesstechnik Verfahren zum Bestimmen des Eindringens von Prüfpartikeln in einen Messbereich
GB2464183A (en) * 2008-09-19 2010-04-14 Singulex Inc Sandwich assay
JP5678045B2 (ja) 2009-06-08 2015-02-25 シンギュレックス・インコーポレイテッド 高感度バイオマーカーパネル
JP2013525820A (ja) 2010-05-06 2013-06-20 シングレクス、インコーポレイテッド 関節リウマチの発症に関するリスクを診断し、病期分類し、予測し、治療レスポンダーを同定するための方法
WO2013181362A1 (fr) * 2012-06-01 2013-12-05 Advanced Analytical Technologies, Inc. Système de détection multicolore pour électrophorèse capillaire multiplex
US9207066B2 (en) 2014-02-14 2015-12-08 Palo Alto Research Center Incorporated Spatial modulation of light to determine dimensional characteristics of objects in a flow path
US9528925B2 (en) 2014-02-14 2016-12-27 Palo Alto Research Center Incorporated Spatial modulation of light to determine object position
US9952033B2 (en) 2014-02-14 2018-04-24 Palo Alto Research Center Incorporated Spatial modulation of light to determine object length
US10451482B2 (en) 2014-02-14 2019-10-22 Palo Alto Research Center Incorporated Determination of color characteristics of objects using spatially modulated light
US9400174B2 (en) 2014-04-07 2016-07-26 Palo Alto Research Center Incorporated Monitor for particle injector
ES2684868B1 (es) * 2017-03-31 2019-07-16 Univ Burgos Dispositivo para determinacion fluorimetrica de aminas terciarias

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US3470373A (en) * 1966-10-18 1969-09-30 Litton Systems Inc Method for analysis and identification of biologic entities by phosphorescence
US3497690A (en) * 1967-09-21 1970-02-24 Bausch & Lomb Method and apparatus for classifying biological cells by measuring the size and fluorescent response thereof
US3586859A (en) * 1970-02-16 1971-06-22 Irwin J Katz Fluorescent cell viability counter
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DE2544575A1 (de) * 1975-10-04 1977-04-07 Fraunhofer Ges Forschung Verfahren zur identifizierung und groessenbestimmung einzelner fluessigkeits- oder gasgetragener partikel

Also Published As

Publication number Publication date
GB2032097B (en) 1982-10-13
US4172227A (en) 1979-10-23
GB2032097A (en) 1980-04-30
DE2929170A1 (de) 1980-06-12
DE2929170C2 (de) 1984-02-09
FR2431696B3 (fr) 1981-05-29

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