FR2373805A1 - Procede et appareil pour monter des coupes de tissus avec un milieu de montage durcissable par la lumiere ultraviolette - Google Patents
Procede et appareil pour monter des coupes de tissus avec un milieu de montage durcissable par la lumiere ultravioletteInfo
- Publication number
- FR2373805A1 FR2373805A1 FR7731406A FR7731406A FR2373805A1 FR 2373805 A1 FR2373805 A1 FR 2373805A1 FR 7731406 A FR7731406 A FR 7731406A FR 7731406 A FR7731406 A FR 7731406A FR 2373805 A1 FR2373805 A1 FR 2373805A1
- Authority
- FR
- France
- Prior art keywords
- mounting
- ultraviolet light
- sample
- fabrics
- polymerizable material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title abstract 2
- 239000012120 mounting media Substances 0.000 title abstract 2
- 239000004744 fabric Substances 0.000 title 1
- 239000000463 material Substances 0.000 abstract 3
- 239000000523 sample Substances 0.000 abstract 3
- 239000002904 solvent Substances 0.000 abstract 2
- 239000012472 biological sample Substances 0.000 abstract 1
- 238000000386 microscopy Methods 0.000 abstract 1
- 238000006116 polymerization reaction Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Microscoopes, Condenser (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
L'invention concerne un procédé et un appareil pour monter des coupes de tissus avec un milieu de montage de ontage durcissable par la lumière ultraviolette. On plonge des coupes minces <<humides>> d'échantillons biologiques 5 sur des lames pour microscopie 3 dans un solvant peu volatil à faible concentration d'une première matière polymérisable; on laisse le solvant s'évaporer pour que la matière polymérisable imprègne l'échantillon et le protège; on dépose une seconde matiere polymérisable sur l'échantillon; et on effectue la polymérisation par exposition aux UV 11 pour obtenir un échantillon encapsulé sur lame. Montage rapide d'échantillons de tissus pour examen immédiat
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US05/749,557 US4120991A (en) | 1976-12-10 | 1976-12-10 | Process for mounting tissue sections with an U.V. light curable mounting medium |
Publications (2)
Publication Number | Publication Date |
---|---|
FR2373805A1 true FR2373805A1 (fr) | 1978-07-07 |
FR2373805B1 FR2373805B1 (fr) | 1984-02-24 |
Family
ID=25014236
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7731406A Granted FR2373805A1 (fr) | 1976-12-10 | 1977-10-19 | Procede et appareil pour monter des coupes de tissus avec un milieu de montage durcissable par la lumiere ultraviolette |
Country Status (12)
Country | Link |
---|---|
US (1) | US4120991A (fr) |
JP (1) | JPS5372639A (fr) |
AU (1) | AU501982B2 (fr) |
BE (1) | BE860067A (fr) |
CA (1) | CA1081591A (fr) |
CH (1) | CH626174A5 (fr) |
DE (1) | DE2754351A1 (fr) |
FR (1) | FR2373805A1 (fr) |
GB (1) | GB1586618A (fr) |
IT (1) | IT1091166B (fr) |
NL (1) | NL7710904A (fr) |
SE (1) | SE439696B (fr) |
Families Citing this family (41)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2939582A1 (de) * | 1979-09-29 | 1981-04-09 | Hoechst Ag, 6000 Frankfurt | Verfahren und einbettungssystem zur einbettung von gewebsproben |
US4284495A (en) * | 1979-12-10 | 1981-08-18 | Newton William A | Coating apparatus and method |
JPS56147038A (en) * | 1980-04-18 | 1981-11-14 | Hitachi Ltd | Pretreatment for supravital stained specimen and automatic classifying apparatus using said pretreating method |
US4523826A (en) * | 1980-12-15 | 1985-06-18 | University Of Pittsburgh | Autoradiography apparatus including a slide rack |
DE3313127A1 (de) * | 1982-04-28 | 1983-11-03 | Bio-Innovations, Camp Hill, Pa. | Vorrichtung zum faerben von biologischen proben |
US4545831A (en) * | 1982-09-13 | 1985-10-08 | The Mount Sinai School Of Medicine | Method for transferring a thin tissue section |
CA1338542C (fr) * | 1982-09-13 | 1996-08-20 | Leonard Ornstein | Composition adhesive pour le montage de specimens |
JPS59157535A (ja) * | 1983-02-28 | 1984-09-06 | Sankyo Co Ltd | 自動標本封入装置 |
US4742690A (en) * | 1983-08-23 | 1988-05-10 | Board Of Regents, The University Of Texas System | Apparatus and method for cryopreparing biological tissue for ultrastructural analysis |
US4745771A (en) * | 1983-08-23 | 1988-05-24 | Board Of Regents, The University Of Texas System | Apparatus and method for cryopreparing biological tissue for ultrastructural analysis |
US5024830A (en) * | 1983-08-23 | 1991-06-18 | The Board Of Regents, The University Of Texas | Method for cryopreparing biological tissue for ultrastructural analysis |
US4676070A (en) * | 1984-11-30 | 1987-06-30 | The Board Of Regents, The University Of Texas | Apparatus and method for cryopreparing biological tissue |
US4510169A (en) * | 1983-08-23 | 1985-04-09 | The Board Of Regents, The University Of Texas | Method and apparatus for cryopreparing biological tissue for ultrastructural analysis |
US4567847A (en) * | 1983-08-23 | 1986-02-04 | Board Of Regents, The University Of Texas System | Apparatus and method for cryopreparing biological tissue for ultrastructural analysis |
US4799361A (en) * | 1983-08-23 | 1989-01-24 | Board Of Regents, The University Of Texas System | Method for cryopreparing biological tissue for ultrastructural analysis |
US4607920A (en) * | 1985-02-06 | 1986-08-26 | Clegg John E | Canada balsam wafers |
US5044165A (en) * | 1986-12-03 | 1991-09-03 | Board Of Regents, The University Of Texas | Cryo-slammer |
DE3707398A1 (de) * | 1987-03-07 | 1988-09-15 | Kulzer & Co Gmbh | Bestrahlungsgeraet zum photopolymerisieren von kunststoff-einbettmassen fuer histologische praeparate |
US5095213A (en) * | 1988-11-03 | 1992-03-10 | Syntex (U.S.A.) Inc. | Method of using an opaque plastic microscope slide for epi-fluorescent microscopy |
JPH04120438A (ja) * | 1990-09-12 | 1992-04-21 | Japan Menburen Technol Kk | プレパラート用封入剤とその製法ならびにプレパラートの製法 |
GB9223588D0 (en) * | 1992-11-11 | 1992-12-23 | Pathway Lab Services Ltd | Staining system |
RU2148952C1 (ru) * | 1996-11-01 | 2000-05-20 | Извозчиков Илья Борисович | Устройство для заключения гистологических и биологических образцов |
US6214054B1 (en) | 1998-09-21 | 2001-04-10 | Edwards Lifesciences Corporation | Method for fixation of biological tissues having mitigated propensity for post-implantation calcification and thrombosis and bioprosthetic devices prepared thereby |
US7374907B1 (en) * | 2000-04-07 | 2008-05-20 | John Voneiff | System and method for automatically processing tissue samples |
US6878168B2 (en) | 2002-01-03 | 2005-04-12 | Edwards Lifesciences Corporation | Treatment of bioprosthetic tissues to mitigate post implantation calcification |
WO2007047131A1 (fr) * | 2005-10-11 | 2007-04-26 | Cytyc Corporation | Procédés et matériels destinés à fixer des échantillons cytologiques |
CN101626682B (zh) | 2006-10-27 | 2014-04-16 | 爱德华兹生命科学公司 | 用于外科植入的生物组织 |
JP5164003B2 (ja) * | 2007-02-19 | 2013-03-13 | 忠文 川本 | 薄切片標本の保存方法 |
US9101691B2 (en) | 2007-06-11 | 2015-08-11 | Edwards Lifesciences Corporation | Methods for pre-stressing and capping bioprosthetic tissue |
US8357387B2 (en) | 2007-12-21 | 2013-01-22 | Edwards Lifesciences Corporation | Capping bioprosthetic tissue to reduce calcification |
NZ602707A (en) | 2010-03-23 | 2014-05-30 | Edwards Lifesciences Corp | Methods of conditioning sheet bioprosthetic tissue |
US8906601B2 (en) | 2010-06-17 | 2014-12-09 | Edwardss Lifesciences Corporation | Methods for stabilizing a bioprosthetic tissue by chemical modification of antigenic carbohydrates |
US9351829B2 (en) | 2010-11-17 | 2016-05-31 | Edwards Lifesciences Corporation | Double cross-linkage process to enhance post-implantation bioprosthetic tissue durability |
US10018540B2 (en) | 2012-04-10 | 2018-07-10 | Rutgers, The State University Of New Jersey | Clearing agent and mounting media for microscopy |
US9464971B2 (en) | 2012-04-10 | 2016-10-11 | Rutgers, The State University Of New Jersey | Clearing agent and mounting medium for microscopy |
CN103364252B (zh) * | 2012-04-10 | 2017-01-18 | 深圳迈瑞生物医疗电子股份有限公司 | 推片染色机的涂片推片洁净方法及装置 |
US10238771B2 (en) | 2012-11-08 | 2019-03-26 | Edwards Lifesciences Corporation | Methods for treating bioprosthetic tissue using a nucleophile/electrophile in a catalytic system |
US9041922B1 (en) | 2013-12-17 | 2015-05-26 | Alessi Technologies, Inc. | System and method for biological specimen mounting |
US10119889B2 (en) | 2013-12-17 | 2018-11-06 | Aquaro Histology, Inc. | System and method for mounting a specimen on a slide |
US20150338164A1 (en) * | 2014-05-23 | 2015-11-26 | Pioneer Hi Bred International Inc | Genetic Profiling Flexible Microplate Drying Systems and Methods |
CN116990101B (zh) * | 2023-09-27 | 2023-12-15 | 四川大学华西医院 | 一种易掉片组织的预处理方法及其多重免疫荧光染色方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3466209A (en) * | 1964-07-15 | 1969-09-09 | Newton G Leveskis | Method of preparing microscope slides using terpolymers of vinyl benzene;methyl methacrylate,and acrylate ester |
DE2424583A1 (de) * | 1973-05-22 | 1974-12-12 | Tetronics Res & Dev Co Ltd | Verfahren und vorrichtungen zum herstellen von spuren aus einem physiologischen fluid auf einer oberflaeche und zur behandlung des fluids mit einem faerbmittel |
US3891327A (en) * | 1973-11-01 | 1975-06-24 | Grace W R & Co | Mounted slides and method of securing a cover glass to a glass slide having a specimen thereon |
GB1454365A (en) * | 1972-10-06 | 1976-11-03 | Mawhinney W H B | Apparatus for treating slide specimens |
FR2335838A1 (fr) * | 1975-12-19 | 1977-07-15 | Technicon Instr | Procede et appareil de transfert, notamment de frottis biologiques aux fins d'examen microscopique |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3546334A (en) * | 1965-05-21 | 1970-12-08 | Lerner Lab Inc | Composition for fixing and protecting a smear of body cells and method of applying same |
US3498860A (en) * | 1966-08-29 | 1970-03-03 | John E P Pickett | Process of mounting precoated cover glass for microscope slides |
US3467617A (en) * | 1966-10-27 | 1969-09-16 | Brunswick Corp | Mounting medium,manufacture and use thereof |
US3679450A (en) * | 1970-03-16 | 1972-07-25 | Roy Nutt | Biopsy method |
-
1976
- 1976-12-10 US US05/749,557 patent/US4120991A/en not_active Expired - Lifetime
-
1977
- 1977-07-08 SE SE7708010A patent/SE439696B/xx not_active IP Right Cessation
- 1977-07-14 CA CA282,736A patent/CA1081591A/fr not_active Expired
- 1977-08-15 AU AU27892/77A patent/AU501982B2/en not_active Expired
- 1977-09-22 IT IT69097/77A patent/IT1091166B/it active
- 1977-09-29 GB GB40598/77A patent/GB1586618A/en not_active Expired
- 1977-10-05 NL NL7710904A patent/NL7710904A/xx not_active Application Discontinuation
- 1977-10-13 JP JP12196277A patent/JPS5372639A/ja active Granted
- 1977-10-19 FR FR7731406A patent/FR2373805A1/fr active Granted
- 1977-10-25 BE BE182019A patent/BE860067A/fr not_active IP Right Cessation
- 1977-12-07 DE DE19772754351 patent/DE2754351A1/de active Granted
- 1977-12-08 CH CH1505777A patent/CH626174A5/de not_active IP Right Cessation
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3466209A (en) * | 1964-07-15 | 1969-09-09 | Newton G Leveskis | Method of preparing microscope slides using terpolymers of vinyl benzene;methyl methacrylate,and acrylate ester |
GB1454365A (en) * | 1972-10-06 | 1976-11-03 | Mawhinney W H B | Apparatus for treating slide specimens |
DE2424583A1 (de) * | 1973-05-22 | 1974-12-12 | Tetronics Res & Dev Co Ltd | Verfahren und vorrichtungen zum herstellen von spuren aus einem physiologischen fluid auf einer oberflaeche und zur behandlung des fluids mit einem faerbmittel |
US3891327A (en) * | 1973-11-01 | 1975-06-24 | Grace W R & Co | Mounted slides and method of securing a cover glass to a glass slide having a specimen thereon |
FR2335838A1 (fr) * | 1975-12-19 | 1977-07-15 | Technicon Instr | Procede et appareil de transfert, notamment de frottis biologiques aux fins d'examen microscopique |
Also Published As
Publication number | Publication date |
---|---|
BE860067A (fr) | 1978-04-25 |
AU501982B2 (en) | 1979-07-05 |
CA1081591A (fr) | 1980-07-15 |
SE439696B (sv) | 1985-06-24 |
SE7708010L (sv) | 1978-06-11 |
AU2789277A (en) | 1979-03-08 |
JPS626177B2 (fr) | 1987-02-09 |
US4120991A (en) | 1978-10-17 |
IT1091166B (it) | 1985-06-26 |
CH626174A5 (fr) | 1981-10-30 |
NL7710904A (nl) | 1978-06-13 |
DE2754351A1 (de) | 1978-06-15 |
GB1586618A (en) | 1981-03-25 |
DE2754351C2 (fr) | 1989-08-31 |
FR2373805B1 (fr) | 1984-02-24 |
JPS5372639A (en) | 1978-06-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
TP | Transmission of property | ||
ST | Notification of lapse |