FR2281569A1 - Assay of thyroid hormone in blood - after complete liberation of protein-bound hormone by pepsin digestion then inactivation of enzyme - Google Patents

Assay of thyroid hormone in blood - after complete liberation of protein-bound hormone by pepsin digestion then inactivation of enzyme

Info

Publication number
FR2281569A1
FR2281569A1 FR7524521A FR7524521A FR2281569A1 FR 2281569 A1 FR2281569 A1 FR 2281569A1 FR 7524521 A FR7524521 A FR 7524521A FR 7524521 A FR7524521 A FR 7524521A FR 2281569 A1 FR2281569 A1 FR 2281569A1
Authority
FR
France
Prior art keywords
hormone
thyroid hormone
protein
blood
inactivation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7524521A
Other languages
French (fr)
Other versions
FR2281569B3 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CENTER LAB MEDICINE
Original Assignee
CENTER LAB MEDICINE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CENTER LAB MEDICINE filed Critical CENTER LAB MEDICINE
Publication of FR2281569A1 publication Critical patent/FR2281569A1/en
Application granted granted Critical
Publication of FR2281569B3 publication Critical patent/FR2281569B3/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Endocrinology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Method of assaying thyroid hormone in blood samples in which the sample is mixed with pepsin at pH 1, it is incubated at a suitable temp. for sufficient time to digest the protein attached to the thyroid hormone, the pH is adjusted to a value at which pepsin is inactive, then assaying the liberated thyroid hormone. Elimination of serum and plasma proteins, which bind the hormone, was only partially successful by earlier techniques. After pepsin digestion, the thyroid hormone is completely liberated.
FR7524521A 1974-08-08 1975-08-06 Assay of thyroid hormone in blood - after complete liberation of protein-bound hormone by pepsin digestion then inactivation of enzyme Granted FR2281569A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US49586974A 1974-08-08 1974-08-08

Publications (2)

Publication Number Publication Date
FR2281569A1 true FR2281569A1 (en) 1976-03-05
FR2281569B3 FR2281569B3 (en) 1978-03-24

Family

ID=23970314

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7524521A Granted FR2281569A1 (en) 1974-08-08 1975-08-06 Assay of thyroid hormone in blood - after complete liberation of protein-bound hormone by pepsin digestion then inactivation of enzyme

Country Status (4)

Country Link
JP (1) JPS5221891A (en)
BE (1) BE832234A (en)
FR (1) FR2281569A1 (en)
SE (1) SE7508819L (en)

Also Published As

Publication number Publication date
DE2535279A1 (en) 1976-02-26
DE2535279B2 (en) 1976-10-28
JPS5221891A (en) 1977-02-18
FR2281569B3 (en) 1978-03-24
BE832234A (en) 1975-12-01
SE7508819L (en) 1976-02-09

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Legal Events

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